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Oral administration of Sauce llorón extract to growing lambs to control gastrointestinal nematodes and Moniezia spp.

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Asian Pacific Journal of Tropical Medicine 2015年第7期8卷 518-523页

作者： Cedillo J Kholif AE Salem AZM Elghandour MMY Vázquez JF Alonso MU Barbabosa A Chagoyán JCV Reyna AG university center of UAEM-Temascaltepec Autonomous University of the Mexico State Dairy science Department National Research Centre faculty of veterinary Medicine and Animal science Autonomous University of the Mexico State Autonomous university of the Tamaulipas Faculty of Engineering and Science

Objective: To explore anthelmintic effects of oral administration of aqueous extract of Sauce llorón(Salix babylonica; SB) against gastrointestinal nematodes and Moniezia spp. Methods: Sixteen Pelibuey male lambs of 3-4 months of age and(23.7±3.3) kg body weight were used in a completely randomized design to be fed a total mixed ration(Control; SB0), or Control plus SB extract using 20(SB20), 40(SB40) and 60(SB60) mL/lamb/day for 45 days. Lambs had a natural gastrointestinal nematodes and Moniezia spp. infection and had never been treated with chemical anthelmintic drugs. Individual faecal samples were collected for ova counting using Mc Master procedure after 0, 7, 14, 21, 30 and 45 days post extract administration. Results: No extract dose×day interactions for both gastrointestinal nematodes and Monieziaspp. egg count were found. Administration of SB extract had a higher effect(quadratic effect, P=0.0064) at dose of 20 mL SB/lamb/day for gastrointestinal nematode eggs during the first 21 days; however, the dose of SB40 tended(linear effect, P=0.0897) to be more effective than the others for Moniezia spp. egg during the first 7 days. Sampling day had a linear(P=0.0436) effect on Moniezia spp. egg count. Conclusions: The aqueous extract of SB could be more effective against nematodes at 20 and at 40 mL/lamb/day for Moniezia spp. The use of the SB extract could represent a promising alternative to synthetic anthelmintics for the treatment of gastrointestinal nematodes and Moniezia spp. in small ruminants from organic and conventional production systems.

关键词： Anthelmintic Extract Moniezia spp. Nematodes

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The influence of meat-and-bone meal and exogenous phytase on growth performance, bone mineralisation and digestibility coefficients of protein(N),amino acids and starch in broiler chickens

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Animal Nutrition 2016年第2期2卷 86-92页

作者： Sonia Y.Liu Aaron J.Cowieson Peter H.Selle Poultry Research Foundation within the faculty of veterinary science The University of Sydney

The objective of this study was to examine the influence of meat-and-bone meal(MBM) and phytase inclusion on growth performance, bone mineralisation and apparent digestibility coefficients of nutrients in broiler chickens offered wheat-based diets. The feeding study comprised 7 dietary treatments: positive control(PC, 9.0% Ca and 4.5% available phosphorous [AvP] in starter, 7.0% Ca and 3.5% AvP in finisher); negative control(NC, 7.2% Ca and 3.0% AvP in starter, 5.2% Ca and 2.0% AvP in finisher) diets incorporating a 3 × 2 factorial array of 3 MBM inclusions(0, 60, 120 g/kg) and 2 levels of phytase supplementation(0 and 1,000 FYT/kg). Each treatment was allocated to 6 replicated pens with 30 birds per pen in an environmentally-controlled deep litter facility. A total of 1,260 one-day-old male Ross 308 chicks were offered starter diets from 1 to 14 days post-hatch and finisher diets from 15 to 36 days posthatch. There were significant(P < 0.05) interactions between MBM inclusions and phytase supplementation on weight gain and feed intake in starter diets. Phytase significantly increased weight gain in diets without MBM and did not influence weight gain in diets with 60 and 120 g/kg MBM. Collectively,increasing MBM inclusion significantly reduced weight gain in starter diets(P < 0.0001). There were dietary interactions(P < 0.01) on toe ash where phytase significantly improved toe ash in diet without MBM and did not influence toe ash in the other two groups of negative control diets. There were no dietary treatment interactions on apparent ileal digestibility coefficients of starch and protein except that diets without MBM had significantly(P < 0.01) lower ileal starch digestibility and diets with 120 g/kg MBM had significantly(P < 0.0001) lower ileal protein digestibility. No dietary influence on ileal fat digestibility was observed. There were dietary interactions on ileal digestibilities of isoleucine, valine and glycine. Phytase significantly increased glycine diges

关键词： Bone mineralisation Digestibility Meat-and-bone meal Protein Starch

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Comment on: Cloning and characterization of porcine aquaporin 1 water channel expressed extensively in the gastrointestinal system

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World Journal of Gastroenterology 2006年第27期12卷 4437-4439页

作者： Ali Mobasheri Molecular Pathogenesis and Connective Tissue Research Groups Department of veterinary Preclinical sciences Faculty of Veterinary ScienceUniversity of LiverpoolLiverpoolUnited Kingdom

TO THE EDITOR Sir, I read with great interest the recently published article in the World Journal of Gastroenterology by Jin and co-workers on the cloning and characterization of porcine aquaporin 1 water channel from the pig liver and studies on its expression in the porcine gastrointestinal system. The authors should be congratulated for making this important and valuable contribution to the field of aquaporin biology and porcine gastrointestinal physiology. However, there are a number of unresolved issues and controversies concerning the expression of aquaporins （especially aquaporin 1） in the gastrointestinal system that are worthy of additional comment and discussion by Jin and co-workers.

关键词： Aquaporin Water Channel Aquaporin 1 Gastro-intestinal system Water transport Glycerol transport Digestion Absorption Secretion

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Beneficial effects of Foeniculum vulgare on ethanol-induced acute gastric mucosal injury in rats

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World Journal of Gastroenterology 2007年第4期13卷 607-611页

作者： Fatih Mehmet Birdane Mustafa Cemek Yavuz Osman Birdane lhami Gülin Mehmet Emin Büyükokuro■lu Department of Internal Medicine Faculty of Veterinary Medicine Afyon Kocatepe University Afyon Turkey Department of Chemistry (Biochemistry Division) Faculty of Science and Arts Afyon Kocatepe University Afyon Turkey Department of Pharmacology and Toxicology Faculty of Veterinary Medicine Afyon Kocatepe University Afyon Turkey Department of Chemistry Faculty of Science and Arts Atatürk University Erzurum Turkey Department of Pharmacology Faculty of Medicine Afyon Kocatepe University Afyon Turkey

AIM： To examine the anti-ulcerogenic and antioxidant effects of aqueous extracts of Foeniculum vu/gare （FVE） on ethanol-induced gastric lesions in rats.METHODS： FVE was administered by gavage at doses of 75, i50 and 300 mg/kg, and famotidine was used at the dose of20mg/*** a 60 min period, all the rats were given 1 mL of ethanol （80%） by gavage. One hour after the administration of ethanol, all groups were sacrificed, and the gastric ulcer index was calculated; whole blood malondialdehyde （MDA） and reduced glutathione （GSH）, serum nitrate, nitrite, ascorbic acid, retinol and β-carotene levels were measured in all the groups.RESULTS： It was found that pretreatment with FVE significantly reduced ethanol-induced gastric damage. This effect of FVE was highest and statistically significant in 300 mg/kg group compared with the control （4.18 ± 2.81 vs 13.15 ± 4.08, P 〈 0.001）. Also, pretreatment with FVE significantly reduced the MDA levels, while significantly increased GSH, nitrite, nitrate, ascorbic acid, retinol and β-carotene levels. CONCLUSION： FVE has clearly a protective effect against ethanol-induced gastric mucosal lesion, and this effect, at least in part, depends upon the reduction in lipid peroxidation and augmentation in the antioxidant activity.

关键词： Foeniculum vulgare Ethanol Rat Ulcer Antioxidant

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Prevalence of Bovine Tuberculosis in Sheep at Sadar and Parbatipur Upazila under Dinajpur District in Bangladesh

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Open Journal of veterinary Medicine 2021年第11期11卷 315-326页

作者： Ahmed Ali Omar S. M. Harun-ur-Rashid Md. Haydar Ali Binti Abubakar Mohamed Head of Lab at Someat Company Mogadishu Somalia faculty of veterinary science in benadir university Mogadishu Somalia faculty of veterinary and Animal science Department of Pathology and Parasitology in Hajee Mohammad Danesh science and Technology university Dinajpur Bangladesh faculty of veterinary Medicine and Animal Husbandry at Horsed International university Mogadishu Somalia

Tuberculosis is Zoonotic disease and infected animals continue to spread the disease to other animals and humans by excreting the germs in milk, meat, feces, and respiratory droplets, this study used bPPD (Bovine purified protein derivative) to observe TST (Tuberculin skin test) reactivity in the Sheep population in the Sadar and Parbatipur Upazilas of Dinajpur District. The research conducted from January to June of 2018. There is no history for sheep tuberculosis in the study area. Overall, 5 percent (7/140) of 140 sheep were positive, 15% (21/140) were doubtful, and 80% (112/140) were negative based on sex, age, breed, and body condition score, while lactation stage and reproduction status prevalence was 6.4 percent, 14.9 percent, and 78.7% positive, doubtful, and negative, respectively. Our findings suggest the presence of a skin test reaction response when employing the bPPD reagent, but this needs to be validated by pathological and serological techniques. Nevertheless, my preliminary investigation suggests that abattoirs must implement effective control systems and raise public awareness.

关键词： Sheep Tuberculosis Tuberculin Skin Test bPPD Reagent

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Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry

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Journal of Pharmaceutical Analysis 2018年第1期8卷 69-74页

作者： Yorick Janssens Frederick Verbeke Nathan Debunne Evelien Wynendaele Kathelijne Peremans Bart De Spiegeleer Drug Quality and Registration (DruQuaR) Group Faculty of Pharmaceutical Sciences Ghent University Department of veterinary Medical Imaging and Small Animal Orthopaedics Faculty of Veterinary Medicine Ghent University

Five different quorum sensing peptides(QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1%(m/v) formic acid as mobile phase. Electrospray ionization(ESI)ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current(TIC) spectra. Non-iodinated peptides and mono-and diiodinated peptides(NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low(2%) to high(57%).

关键词： Peptide iodination LC–UV/MS Quorum sensing Bolton-Hunter

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Oyster is an effective transmission vehicle for Cryptosporidium infection in human

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Asian Pacific Journal of Tropical Medicine 2016年第6期9卷 544-547页

作者： Chantira Sutthikornchai Supaluk Popruk Kamlang Chumpolbanchorn Woraporn Sukhumavasi Yaowalark Sukthana Department of Protozoology Faculty of Tropical Medicine Mahidol University Mahidol university International College Parasitology Unit Department of Pathology Faculty of Veterinary Science Chulalongkorn University

Objective: To determine the ability of oysters to trap and maintain viable Cryptosporidium oocysts, and the feasibility of Cryptosporidium multiplication in oysters’ organs. Methods: Seventy oysters were raised in experimentally seeded natural seawater for up to 3 months, with weekly oocysts inoculations. Cryptosporidium oocysts, viable and non-viable, as well as other stages were detected using two immunofluorescence vital staining techniques(SporoGlo and Merifluor~) with confocal microscopy. Viability rate at various times after inoculations were calculated. Results: Cryptosporidium oocysts were found most concentrated in oysters’ digestive organs than in gill and water inside the oysters. Oocysts numbers were 857.33 at 24 h after inoculation and strikingly decreased to 243.00 and 126.67 oocysts at 72 h and 7 days, respectively. The oocysts in oyster were also less viable over time; 70%, 60% and 30% viable at 24 hrs, 72 hrs and 7days after inoculation, respectively. At 77 days, the number of oocysts was very low and none was found at 84 days onwards. Although some oocysts were ruptured with released sporozoites, there was no evidence throughout the study of sporozoites multiplication to indicate that oyster is a biological host. Despite the significant reduction in oocysts number after 7 days of inoculation, the remained viable oocysts can still cause cryptosporidiosis. Conclusion: The findings confirm that Cryptosporidium parvum does not multiply in oyster, and is therefore not a biological host. Nevertheless, the results suggest that oyster can be an effective transmission vehicle for Cryptosporidium oocysts, especially within 24-72 h of contamination, with viable oocysts present at up to 7 days post infection. Unless consuming well-cooked oyster dishes, eating raw oyster remains a public health concern and at least 3 days of depuration in clean sea water prior to consumption is recommended.

关键词： Cryptosporidium Oysters IFAT Crassostrea belcheri

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Determining the IgG concentrations in bovine colostrum and calf sera with a novel enzymatic assay

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Journal of Animal science and Biotechnology 2019年第1期10卷 239-247页

作者： M.Drikic C.Windeyer S.Olsen Y.Fu L.Doepel J.De Buck Department Production Animal Health Faculty of Veterinary MedicineUniversity of Calgary

Background: Immune protection in newborn calves relies on a combination of the timing,volume and quality of colostrum consumed by the calf after *** quality colostrum with inadequate immunoglobulin concentration contributes to failed transfer of passive immunity in calves,leading to higher calf morbidity and ***,estimating colostrum quality and ensuring the transfer of passive immunity on farm is of critical ***,there are no on-farm tools that directly measure immunoglobulin content in colostrum or *** aim of this study was to apply a novel molecular assay,split trehalase immunoglobulin G assay(STIGA),to directly estimate immunoglobulin content in dairy and beef colostrum and calf sera,and to examine its potential to be developed as on-farm *** STIGA is based on a split version of trehalase TreA,an enzyme that converts trehalose into glucose,enabling the use of a common glucometer for signal *** a first study,60 dairy and64 beef colostrum and 83 dairy and 84 beef calf sera samples were tested with STIGA,and the resulting glucose production was measured and compared with radial immunodiffusion,the standard method for measuring immunoglobulin ***: Pearson correlation coefficients between the methods were determined and the sensitivity,specificity,and accuracy of the test were calculated for different colostrum quality and failed transfer of passive immunity cut-off *** correlations of the STIGA measured by colorimetric enzymatic reaction compared to radial immunodiffusion for dairy and beef colostrum were 0.72 and 0.73,respectively,whereas the correlations for dairy and beef sera were 0.9 and 0.85,***,STIGA was tested in a blinded study with fresh colostrum and serum samples where the correlation coefficient was 0.93 and 0.94,***,the performance of STIGA followed by glucometer readings resulted in correlations with radial immunodiffusion of 0.7 and

关键词： Cattle Colostrum Immunoglobulins Passive immunity Radial immunodiffusion Split trehalase

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C-glycosyl flavonoid orientin alleviates learning and memory impairment by radiofrequency electromagnetic radiation in mice via improving antioxidant defence mechanism

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Asian Pacific Journal of Tropical Biomedicine 2019年第12期9卷 518-523页

作者： Isaac O.Akefe Ibrahim L.Yusuf Victoria A.Adegoke Department of Physiology Biochemistry and PharmacologyFaculty of Veterinary MedicineUniversity of JosNigeria Department of veterinary Pharmacology and Toxicology University of MaiduguriNigeria Department of science and Laboratory Technology Ekiti State UniversityNigeria

Objective: To investigate the antioxidant efficacy of oral orientin on 900 MHz radiofrequencyelectromagnetic radiation-induced oxidative stress in ***: The mice were randomly allotted into 5 groups consisting of 7 mice each. The deionised water and radiofrequency electromagnetic radiations(RF-EMR) groups were administered with deionised water while orientin was administered to the RF-EMR + low dose of orientin group(10 mg/kg), RF-EMR + high dose of orientin group(20 mg/kg) and high dose of orientin group(20 mg/kg). All the groups except deionised water and high dose groups were exposed to 900 MHz radiofrequency-electromagnetic radiation for 28 consecutive days(1 h/day). Learning and memory was assessed via the step-down inhibitory avoidance task. Activities of lipid prexidation and antioxidant enzymes were measured using ***: Radiofrequency electromagnetic radiation caused impairment in learning and memory and reduced activities of brain antioxidant enzymes, increased lipoperoxidation and corticosterone concentration as well as histopathological aberrations in the hippocampal tissues. Conversely, orientin alleviated learning and memory deficit, improved the activities of endogenous antioxidant enzymes and mitigated brain lipoperoxidation and neuronal degeneration in mice exposed to radiofrequency electromagnetic radiation. Conclusions: Orientin alleviates learning and memory impairment due to radiofrequency electromagnetic radiation in mice by improving antioxidant defence mechanism and may be considered as a promising therapeutic agent for improving the antioxidant system of people living in radiofrequency electromagnetic radiation-prone environment.

关键词： Orientin Antioxidants Radiofrequency electromagnetic radiation Mice Oxidative stress

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Molecular detection of Anaplasma marginale and Anaplasma ovis in sheep and goat in west highland pasture of Iran

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Asian Pacific Journal of Tropical Biomedicine 2017年第5期7卷 455-459页

作者： Ali Yousefi Sadegh Rahbari Parviz Shayan Zainab Sadeghi-dehkordi Alireza Bahonar Department of Microbiology Science and Research BranchIslamic Azad University Department of Parasitology Faculty of Veterinary MedicineUniversity of Tehran Department of Parasitology Faculty of Paraveterinary MedicineBu-Ali Sina University Department of Food Hygiene and Quality Control Faculty of Veterinary MedicineUniversity of Tehran

Objective:To determine the prevalence of Anaplasma marginale(***) and Anaplasma ovis from sheep and goat in different highland pasture in west of ***:From July 2015 to October 2015,370 blood samples of sheep and goat were collected from different regions in Hamedan province,*** DNA extracted from blood and subsequently,16 S r RNA and MSP4 genes were analyzed by nested-PCR,semi nested-PCR and RFLP ***:In the PCR assessment,overall 27.5%(102/370) of sheep and goat were positive for Anaplasma ovis and *** infection,which was lower than reports from tropical and subtropical regions of *** analysis(the Chi-square test) did not show any significant relation between infection and variables such as location,tick infestation age and sex(P > 0.05).No significant correlation between the altitude and the Anaplasma species infection was found(Mann–Whitney test:P > 0.05).However,Anaplasma infection in goat significantly is more than the sheep(P = 0.008).Conclusions:The ecological changes affect the frequency and distribution of Anaplasma ***,our results indicate that sheep as potential reservoirs of ***.

关键词： Anaplasma PCR Sheep Goat Iran

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