Molecular detection of Anaplasma marginale and Anaplasma ovis in sheep and goat in west highland pasture of Iran

作     者：Ali Yousefi Sadegh Rahbari Parviz Shayan Zainab Sadeghi-dehkordi Alireza Bahonar 

作者机构：Department of MicrobiologyScience and Research BranchIslamic Azad University Department of ParasitologyFaculty of Veterinary MedicineUniversity of Tehran Department of ParasitologyFaculty of Paraveterinary MedicineBu-Ali Sina University Department of Food Hygiene and Quality ControlFaculty of Veterinary MedicineUniversity of Tehran 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2017年第7卷第5期

页      面：455-459页

核心收录：

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：financially supported by Science and Research Branch of Islamic Azad University Tehran Iran 

主　　题：Anaplasma PCR Sheep Goat Iran 

摘      要：Objective:To determine the prevalence of Anaplasma marginale(***) and Anaplasma ovis from sheep and goat in different highland pasture in west of ***:From July 2015 to October 2015,370 blood samples of sheep and goat were collected from different regions in Hamedan province,*** DNA extracted from blood and subsequently,16 S r RNA and MSP4 genes were analyzed by nested-PCR,semi nested-PCR and RFLP ***:In the PCR assessment,overall 27.5%(102/370) of sheep and goat were positive for Anaplasma ovis and *** infection,which was lower than reports from tropical and subtropical regions of *** analysis(the Chi-square test) did not show any significant relation between infection and variables such as location,tick infestation age and sex(P 0.05).No significant correlation between the altitude and the Anaplasma species infection was found(Mann–Whitney test:P 0.05).However,Anaplasma infection in goat significantly is more than the sheep(P = 0.008).Conclusions:The ecological changes affect the frequency and distribution of Anaplasma ***,our results indicate that sheep as potential reservoirs of ***.