Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry

作     者：Yorick Janssens Frederick Verbeke Nathan Debunne Evelien Wynendaele Kathelijne Peremans Bart De Spiegeleer 

作者机构：Drug Quality and Registration (DruQuaR) Group Faculty of Pharmaceutical Sciences Ghent University Department of Veterinary Medical Imaging and Small Animal Orthopaedics Faculty of Veterinary Medicine Ghent University 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2018年第8卷第1期

页      面：69-74页

核心收录：

学科分类：10[医学] 

基　　金：the‘Research Foundation–Flanders(FWO)’(Grant no.1S21017N to Nathan Debunne) the‘Institute for the Promotion of Innovation through Science and Technology in Flanders(IWT-Vlaanderen)’(Grant no.131356 to Frederick Verbeke) 

主　　题：Peptide iodination LC–UV/MS Quorum sensing Bolton-Hunter 

摘      要：Five different quorum sensing peptides(QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1%(m/v) formic acid as mobile phase. Electrospray ionization(ESI)ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current(TIC) spectra. Non-iodinated peptides and mono-and diiodinated peptides(NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low(2%) to high(57%).