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Characterization of transgenic wheat lines expressing maize ABP7 involved in kernel development

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Journal of Integrative Agriculture 2023年第2期22卷 389-399页

作者： Zaid CHACHAR Siffat Ullah KHAN ZHANG Xu-huan LENG Peng-fei ZONG Na ZHAO Jun faculty of maize functional genomics National Key Facility for Crop Gene Resources and Genetic Improvement/Biotechnology Research InstituteChinese Academy of Agricultural SciencesBeijing 100080P.R.China

Wheat is one of the major food crops in the *** validation of the genes in increasing the grain yield of wheat by genetic engineering is essential for feeding the ever-growing global *** study investigated the role of ABP7,a bHLH transcription factor from maize involved in kernel development,in regulating grain yield-related traits in transgenic *** characterization showed that transgenic lines HB123 and HB287 contained multicopy integration of ABP7 in the genome with higher transgene *** the same time,QB205 was a transgenic event of single copy insertion with no significant difference in ABP7 expression compared to wild-type(WT) *** under field conditions showed that ABP7 over-expressing transgenic lines HB123 and HB287 exhibited improved grain yield-related traits(e.g.,grain number per spike,grain weight per spike,thousand-grain weight,grain length,and grain width) and increased grain yield per plot,compared to WT plants,whereas line QB205 did *** addition,total chlorophyll,chlorophyll a,chlorophyll b,and total soluble sugars were largely increased in the flag leaves of both HB123and HB287 transgenic lines compared to the *** results strongly suggest that ABP7 positively regulates yieldrelated traits and plot grain yield in transgenic ***,ABP7 can be utilized in wheat breeding for grain yield improvement.

关键词： transgenic wheat ABP7 kernel development grain weight grain width

Comparative transcriptome profiling of two maize near-isogenic lines differing in the allelic state for bacterial brown spot disease resistance

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Journal of Integrative Agriculture 2015年第4期14卷 610-621页

作者： WU Xiao-jun Xu Li ZHAO Pan-feng LI Na WU Lei HE Yan WANG Shou-cai National maize Improvement Center of China/Key Laboratory of Crop genomics and Genetic of maize China Agricultural University

The bacterial brown spot disease（BBS）, caused primarily by Pseudomonas syringae pv. syringae van Hall（Pss）, reduces plant vigor, yield and quality in maize. To reveal the nature of the defense mechanisms and identify genes involved in the effective host resistance, the dynamic changes of defense transcriptome triggered by the infection of Pss were investigated and compared between two maize near-isogenic lines（NILs）. We found that Pss infection resulted in a sophisticated transcriptional reprogramming of several biological processes and the resistant NIL employed much faster defense responses than the susceptible NIL. Numerous genes encoding essential components of plant basal resistance would be able to be activated in the susceptible NIL, such as PEN1, PEN2, PEN3, and EDR1, however, in a basic manner, such resistance might not be sufficient for suppressing Pss pathogenesis. In addition, the expressions of a large number of PTI-, ETI-, PR-, and WRKY-related genes were pronouncedly activated in the resistant NIL, suggesting that maize employ a multitude of defense pathways to defend Pss infection. Six R-gene homologs were identified to have significantly higher expression levels in the resistant NIL at early time point, indicating that a robust surveillance system（gene-to-gene model） might operate in maize during Pss attacks, and these homolog genes are likely to be potential candidate resistance genes involved in BBS disease resistance. Furthermore, a holistic group of novel pathogen-responsive genes were defined, providing the repertoire of candidate genes for further functional characterization and identification of their regulation patterns during pathogen infection.

关键词： maize(Zea mays L.) bacterial brown spot disease RNA-Seq analysis disease resistance

maize ABP2 enhances tolerance to drought and salt stress in transgenic Arabidopsis

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Journal of Integrative Agriculture 2018年第11期17卷 2379-2393页

作者： ZONG Na LI Xing-juan WANG Lei WANG Ying WEN Hong-tao LI Ling ZHANG Xia FAN Yun-liu ZHAO Jun National Key Facility for Crop Gene Resources and Genetic Improvement/faculty of maize functional genomics Biotechnology Research Institute Chinese Academy of Agricultural Sciences Guangdong Key Laboratory of Biotechnology for Plant Development/College of Life Science South China Normal University

Abiotic stresses, especially drought and salt, severely affect maize production, which is one of the most important cereal crops in the world. Breeding stress-tolerant maize through biotechnology is urgently needed to maintain maize production. Therefore, it is important to identify new genes that can enhance both drought and salt stress tolerance for molecular breeding. In this study, we identified a maize ABA （abscisic acid）-responsive element （ABRE） binding protein from a 17-day post-pollination （dpp） maize embryo cDNA library by yeast one-hybrid screen using the ABRE2 sequence of the maize Cat1 gene as bait. This protein, designated, ABRE binding protein 2 （ABP2）, belongs to the bZIP transcription factor family. Endogenous expression of ABP2 in maize can be detected in different tissues at various development stages, and can be induced by drought, salt, reactive oxygen species （ROS）-generating agents, and ABA treatment. Constitutive expression of ABP2 in transgenic Arabidopsis plants enhanced tolerance to drought and salt stress, and increased sensitivity to ABA. In exploring the mechanism by which ABP2 can stimulate abiotic stress tolerance, we found that ROS levels were reduced and expression of stress-responsive and carbon metabolism-related genes was enhanced by constitutive ABP2 expression in transgenic plants. In Short, we identified a maize bZIP transcription factor which can enhance both drought and salt tolerance of plants.

关键词： ABP2 maize transcription factor drought salt transgenic Arabidopsis

Combined genome-wide association study and transcriptome analysis reveal candidate genes for resistance to Fusarium ear rot in maize

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Journal of Integrative Plant Biology 2020年第10期62卷 1535-1551页

作者： Lishan Yao Yanmei Li Chuanyu Ma Lixiu Tong Feili Du Mingliang Xu State Key Laboratory of Plant Physiology and Biochemistry College of Agronomy and BiotechnologyNational Maize Improvement CenterCenter for Crop Functional Genomics and Molecular BreedingChina Agricultural UniversityBeijing 100193China

Fusarium ear rot,caused by Fusarium verti-cillioides,is a devastating fungal disease in maize that re-duces yield and quality;moreover,*** pro-duces fumonisin mycotoxins,which pose serious threats to human and animal ***,we performed a genome-wide association study(GWAS)under three en-vironmental conditions and identified 34 single-nucleotide polymorphisms(SNPs)that were significantly associated with Fusarium ear rot *** reference to the maize B73 genome,69 genes that overlapped with or were adjacent to the significant SNPs were identified as potential resistance genes to Fusarium ear *** transcriptomes of the most resistant and most susceptible lines during the very early response to Fusarium ear rot,we detected many differentially expressed genes enriched for pathways related to plant immune responses,such as plant hormone signal transduction,phenylpropanoid bio-synthesis,and cytochrome P450 *** than one-fourth of the potential resistance genes detected in the GWAS were differentially expressed in the tran-scriptome analysis,which allowed us to predict numbers of candidate genes for maize resistance to ear rot,in-cluding genes related to plant hormones,a MAP kinase,a PR5-like receptor kinase,and heat shock *** propose that maize plants initiate early immune responses to Fusarium ear rot mainly by regulating the growth-defense balance and promoting biosynthesis of defense compounds.

关键词： resistance analysis Fusarium

ZmDRR206 functions in maintaining cell wall integrity during maize seedling growth and defense response to external stresses

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The Crop Journal 2023年第6期11卷 1649-1664页

作者： Tao Zhong Suining Deng Mang Zhu Xingming Fan Mingliang Xu Jianrong Ye National maize Improvement Center/Center for Crop functional genomics and Molecular Breeding China Agricultural UniversityBeijing 100193China Institute Food Crops Yunnan Academy of Agricultural SciencesKunming 650205YunnanChina

Plants adaptively change their cell wall composition and structure during their growth,development,and interactions with environmental *** proteins(DIRs)contribute to environmental adaptations by dynamically reorganizing the cell wall and/or by generating defense compounds.A maize DIR,ZmDRR206,was previously reported to play a dominant role in regulation of storage nutrient accumulation in endosperm during maize kernel *** we show that ZmDRR206 mediates maize seedling growth and disease resistance by coordinately regulating biosynthesis of cell wall components for cell-wall integrity(CWI)*** of ZmDRR206 was induced in maize seedlings upon pathogen ***206 overexpression in maize resulted in reduced seedling growth and photosynthetic activity but increased disease resistance and drought tolerance,revealing a tradeoff between growth and ***,ZmDRR206 overexpression reduced the contents of primary metabolites and down-regulated genes involved in photosynthesis,while increasing the contents of major cell wall components,defense phytohormones,and defense metabolites,and up-regulated genes involved in defense and cell-wall biosynthesis in ***206-overexpressing seedlings were resistant to cell-wall stress imposed by isoxaben,and ZmDRR206 physically interacted with ZmCesA10,which is a cellulose synthase *** findings suggest a mechanism by which ZmDRR206 coordinately regulates biosynthesis of cell-wall components for CWI maintenance during maize seedling growth,and might be exploited for breeding strong disease resistance in maize.

关键词： Cell-wall integrity Cell-wall biosynthesis Dirigent protein ZmDRR206 Defense response Seedling growth

Genome assembly of the maize inbred line A188 provides a new reference genome for functional genomics

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The Crop Journal 2022年第1期10卷 47-55页

作者： Fei Ge Jingtao Qu Peng Liu Lang Pan Chaoying Zou Guangsheng Yuan Cong Yang Guangtang Pan Jianwei Huang Langlang Ma Yaou Shen Key Laboratory of Biology and Genetic Improvement of maize in Southwest Region Maize Research InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina Berry genomics Corporation Beijing 100015China

The current assembled maize genomes cannot represent the broad genetic diversity of maize *** more genome sequences is critical for constructing a pan-genome and elucidating the linkage between genotype and phenotype in *** we describe the genome sequence and annotation of A188,a maize inbred line with high phenotypic variation relative to other lines,acquired by single-molecule sequencing and optical genome *** assembled a 2210-Mb genome with a scaffold N50 size of 11.61 million bases(Mb),compared to 9.73 Mb for B73 and 10.2 Mb for *** on the B73_Ref Gen_V4 genome,295 scaffolds(2084.35 Mb,94.30%of the final genome assembly)were anchored and oriented on ten *** analysis revealed that~30%of the predicted A188 genes showed large structural divergence from B73,Mo17,and W22 genomes,which causes high protein divergence and may lead to phenotypic variation among the four inbred *** a line with high embryonic callus(EC)induction capacity,A188 provides a convenient tool for elucidating the molecular mechanism underlying the formation of EC in *** our new A188 genome with previously reported QTL and RNA sequencing data revealed eight genes with large structural variation and two differentially expressed genes playing potential roles in maize EC induction.

关键词： maize Embryonic callus A188 Genome assembly Single-molecule sequencing

The Auxin-Regulated Protein ZmAuxRPI Coordinates the Balance between Root Growth and Stalk Rot Disease Resistance in maize

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Molecular Plant 2019年第3期12卷 360-373页

作者： Jianrong Ye Tao Zhong Dongfeng Zhang Chuanyu Ma Lina Wang Lishan Yao Qianqian Zhang Mang Zhu Mingliang Xu State Key Laboratory of Plant Physiology and Biochemistry/National maize Improvement Center/College of Agronomy and Biotechnology/Center for Crop functional genomics and Molecular Breeding China Agricultural University2 West Yuanmingyuan RoadBeijing 100193P.R.China

To optimize fitness, plants must efficiently allocate their resources between growth and defense. Although phytohormone crosstalk has emerged as a major player in balancing growth and defense, the genetic basis by which plants man age this balance remai ns elusive. We previously ide ntified a quantitative disease . resistance locus, qRfg2, in maize (Zea mays) that protects against the fungal disease Gibberella stalk rot. Here, through map-based cloning, we demonstrate that the causal gene at qRfg2 is ZmAuxRPI, which encodes a plastid stroma-localized auxin-regulated protein. ZmAuxRPI responded quickly to pathogen challenge with a rapid yet transient reduction in expression that led to arrested root growth but enhanced resista nee to Gibberella stalk rot and Fusarium ear rot. ZmAuxRPI was show n to promote the biosynthesis of indole-3-acetic acid (IAA), while suppressing the formation of benzoxazinoid defense compounds. ZmAuxRPI presumably acts as a resource regulator modulating indole-3-glycerol phosphate and/or indole flux at the branch point between the IAA and benzoxazinoid biosynthetic pathways. The concerted interplay between IAA and benzoxazinoids can regulate the growth-defense balance in a timely and efficient manner to optimize plant fitness.

关键词： maize quantitative disease resistance Gibberella stalk rot Fusarium ear rot indole-3-acetic acid benzoxazinoids

Kernel metabolites depict the diversity of relationship between maize hybrids and their parental lines

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The Crop Journal 2021年第1期9卷 181-191页

作者： Yang Xu Ying Ma Xin Wang Cheng Li Xuecai Zhang Pengcheng Li Zefeng Yang Chenwu Xu Key Laboratory of Plant functional genomics of the Ministry of Education Jiangsu Key Laboratory of Crop Genomics and Molecular BreedingJiangsu Co-Innovation Center for Modern Production Technology of Grain CropsCollege of AgricultureYangzhou UniversityYangzhou 225009JiangsuChina International maize and Wheat Improvement Center(CIMMYT) 06600 MexicoD.F.Mexico

As the end products of cellular regulatory processes,metabolites provide the link between genotypes and *** have been widely applied for functional gene detection and phenotype prediction in maize,there is little research focusing on the genetic information of metabolites per ***,we performed genetic analyses for the kernel metabolites of 11 parental inbred lines of six representative maize varieties,including Zhongdan 2,Danyu 13,Yedan 13,Zhengdan 958,Xianyu 355,and Suyu 16,as well as their 26 reciprocal *** identified a total of 208 metabolites in maize kernels using untargeted metabolite profiling *** cluster analysis and principal component analysis indicated that kernel metabolites could distinguish hybrids from their *** of variance further revealed that 163 metabolites exhibited significant differences between parents and hybrids,and 40 metabolites showed significant differences between reciprocal *** also investigated the genetic effects and heterosis for each *** taking all hybrids into consideration,about two-thirds of all metabolites displayed overdominant with 36.8%and 31%of them displaying positive overdominant and negative overdominant,***,27.5%and 20.4%of all hybrid combinations showed significant mid-parent heterosis and over-parent heterosis,*** findings revealed that kernel metabolites exhibited the diversity of relationship between maize hybrids and their parental ***,we identified 25 significant metabolicmarkers related to 11 agronomic traits using the LASSO *** metabolic markers were associated with more than one trait *** results provide a genetic basis for further utilization of metabolites in the genetic improvement of maize.

关键词： maize Kernel Metabolite Reciprocals Heterosis

MP3RNA-seq:Massively parallel 3’end RNA sequencing for high-throughput gene expression profiling and genotyping

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Journal of Integrative Plant Biology 2021年第7期63卷 1227-1239页

作者： Jian Chen Xiangbo Zhang Fei Yi Xiang Gao Weibin Song Haiming Zhao Jinsheng Lai State Key Laboratory of Plant Physiology and Biochemistry National Maize Improvement CenterDepartment of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China Center for Crop functional genomics and Molecular Breeding China Agricultural UniversityBeijing 100193China

Transcriptome deep sequencing(RNA‐seq)hasbecome a routine method for global geneexpression ***,its application tolarge‐scale experiments remains limited by costand labor *** we describe amassively parallel 3′end RNA‐seq(MP3RNA‐seq)method that introduces unique samplebarcodes during reverse transcription to permitsample pooling immediately following this ***3RNA‐seq allows for handling of hun-dreds of samples in a single experiment,at acost of about$6 per sample for libraryconstruction and ***3RNA‐seq iseffective for not only high‐throughput geneexpression profiling,but also *** its utility,we applied MP3RNA‐seqto 477 double haploid lines of *** iden-tified 19,429 genes expressed in at least 50%ofthe lines and 35,836 high‐quality singlenucleotide polymorphisms for *** with these data,we performedexpression and agronomic trait quantitativetrait locus(QTL)mapping and identified 25,797expression QTLs for 15,335 genes and 21 QTLsfor plant height,ear height,and relative *** conclude that MP3RNA‐seq is highlyreproducible,accurate,and sensitive forhigh‐throughput gene expression profiling andgenotyping,and should be generally applicableto most eukaryotic species.

关键词： gene expression profiling genotyping maize MP3RNA‐seq quantitative trait loci