MP3RNA-seq:Massively parallel 3’end RNA sequencing for high-throughput gene expression profiling and genotyping

作     者：Jian Chen Xiangbo Zhang Fei Yi Xiang Gao Weibin Song Haiming Zhao Jinsheng Lai Jian Chen;Xiangbo Zhang;Fei Yi;Xiang Gao;Weibin Song;Haiming Zhao;Jinsheng Lai

作者机构：State Key Laboratory of Plant Physiology and BiochemistryNational Maize Improvement CenterDepartment of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China Center for Crop Functional Genomics and Molecular BreedingChina Agricultural UniversityBeijing 100193China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2021年第63卷第7期

页      面：1227-1239页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by grants from National NaturalScience Foundation of China(31421005) National Key Re-search and Development Program(2016YFD0100404,2016YFD0101804) National Postdoctoral Program for Innovative Talents(BX201600149) China PostdoctoralScience Foundation(2017M611049) 

主　　题：gene expression profiling genotyping maize MP3RNA‐seq quantitative trait loci 

摘      要：Transcriptome deep sequencing(RNA‐seq)hasbecome a routine method for global geneexpression ***,its application tolarge‐scale experiments remains limited by costand labor *** we describe amassively parallel 3′end RNA‐seq(MP3RNA‐seq)method that introduces unique samplebarcodes during reverse transcription to permitsample pooling immediately following this ***3RNA‐seq allows for handling of hun-dreds of samples in a single experiment,at acost of about$6 per sample for libraryconstruction and ***3RNA‐seq iseffective for not only high‐throughput geneexpression profiling,but also *** its utility,we applied MP3RNA‐seqto 477 double haploid lines of *** iden-tified 19,429 genes expressed in at least 50%ofthe lines and 35,836 high‐quality singlenucleotide polymorphisms for *** with these data,we performedexpression and agronomic trait quantitativetrait locus(QTL)mapping and identified 25,797expression QTLs for 15,335 genes and 21 QTLsfor plant height,ear height,and relative *** conclude that MP3RNA‐seq is highlyreproducible,accurate,and sensitive forhigh‐throughput gene expression profiling andgenotyping,and should be generally applicableto most eukaryotic species.