Combined genome-wide association study and transcriptome analysis reveal candidate genes for resistance to Fusarium ear rot in maize

作     者：Lishan Yao Yanmei Li Chuanyu Ma Lixiu Tong Feili Du Mingliang Xu Lishan Yao;Yanmei Li;Chuanyu Ma;Lixiu Tong;Feili Du;Mingliang Xu

作者机构：State Key Laboratory of Plant Physiology and BiochemistryCollege of Agronomy and BiotechnologyNational Maize Improvement CenterCenter for Crop Functional Genomics and Molecular BreedingChina Agricultural UniversityBeijing 100193China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2020年第62卷第10期

页      面：1535-1551页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 0904[农学-植物保护] 071007[理学-遗传学] 0901[农学-作物学] 0703[理学-化学] 090401[农学-植物病理学] 0902[农学-园艺学] 090402[农学-农业昆虫与害虫防治] 090102[农学-作物遗传育种] 

基　　金：We thank Professor Jianyu Wu(Henan Agricultural University)for providing Fusarium verticillioides wethank Professor Xiaohong Yang(China Agricultural University)for providing GwAS population and SNP data This work was funded by the Ministry of Agriculture of China(2018ZX08009-04B) 

主　　题：resistance analysis Fusarium 

摘      要：Fusarium ear rot,caused by Fusarium verti-cillioides,is a devastating fungal disease in maize that re-duces yield and quality;moreover,*** pro-duces fumonisin mycotoxins,which pose serious threats to human and animal ***,we performed a genome-wide association study(GWAS)under three en-vironmental conditions and identified 34 single-nucleotide polymorphisms(SNPs)that were significantly associated with Fusarium ear rot *** reference to the maize B73 genome,69 genes that overlapped with or were adjacent to the significant SNPs were identified as potential resistance genes to Fusarium ear *** transcriptomes of the most resistant and most susceptible lines during the very early response to Fusarium ear rot,we detected many differentially expressed genes enriched for pathways related to plant immune responses,such as plant hormone signal transduction,phenylpropanoid bio-synthesis,and cytochrome P450 *** than one-fourth of the potential resistance genes detected in the GWAS were differentially expressed in the tran-scriptome analysis,which allowed us to predict numbers of candidate genes for maize resistance to ear rot,in-cluding genes related to plant hormones,a MAP kinase,a PR5-like receptor kinase,and heat shock *** propose that maize plants initiate early immune responses to Fusarium ear rot mainly by regulating the growth-defense balance and promoting biosynthesis of defense compounds.