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Reproductive toxicity of enrofloxacin in Caenorhabditis elegans involves oxidative stress-induced cell apoptosis

Journal of Environmental Sciences 2023年第5期127卷 726-737页

作者： Jiahao Huang Lizi Liao Guowei Wang Zhongkun Du Zhengxing Wu Key laboratory of Molecular Biophysics Ministry of EducationCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China School of Environmental Ecology and Biological Engineering Wuhan Institute of TechnologyWuhan 430205China Key laboratory of Green Chemical Engineering Process of Ministry of Education Wuhan 430205China College of Resources and Environment Shandong Agricultural UniversityTaian 271018China

Fluoroquinolone antibiotics(FQs)that persist and bioaccumulate in the environment have aroused people’s great ***,we studied the adverse effects of FQs in soil animals of Caenorhabditis elegans via food-chronically *** result shows *** exposed to FQs exhibited reproductive toxicity with small-brood size and low-egg *** study the underlying mechanism,we conduct a deep investigation of enrofloxacin(ENR),one of the most frequently detected FQs,on nematodes which is one of commonly used animal indicator of soil *** concentration-effect curves simulated by the Hill model showed that the half effect concentrations(EC50)of ENR were(494.3±272.9)μmol/kg and(107.4±30.9)μmol/kg for the brood size and the hatchability,*** gene expression between the control and the ENR-exposure group enriched with the oxidative stress and cell apoptosis *** results together with the enzyme activity in oxidative stress and the cell corpses suggested that ENR-induced reproductive toxicity was related to germ cell apoptosis under oxidative *** risk quotients of some soil and livestock samples were calculated based on the threshold value of EC10 for the egg hatchability(2.65μmol/kg).The results indicated that there was possible reproductive toxicity on the nematodes in certain agricultural soils for the *** study suggested that chronic exposure to FQs at certain levels in environment would induce reproductive toxicity to the nematodes and might reduce the soil sustainability,alarming the environment risks of antibiotics abuse.

关键词： Environmental toxicity Nematodes Chronic exposure Oxidative stress response Germ cell apoptosis

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CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma

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Signal Transduction and Targeted Therapy 2021年第10期6卷 3142-3154页

作者： Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen National Translational Science Center for Molecular Medicine&Department of Cell Biology Fourth Military Medical UniversityXi'an 710032China School of Medicine Shanghai UniversityShanghai 200444China Zhongnan Hospital of Wuhan University Wuhan 430071China Division of HIV/AIDS and Sex-transmitted Virus Vaccines Institute for Biological Product ControlNational Institutes for Food and Drug Control(NIFDC)and WHO Collaborating Center for Standardization and Evaluation of BiologicalsBeijing 102629China Institute of laboratory Animals Science Chinese Academy of Medical SciencesBeijing 100071China NHC Key laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 100871China Beijing Institute of Biotechnology Beijing 100871China Department of Clinical Immunology Xijing HospitalFourth Military Medical UniversityXi'an 710032China Beijing Institute of Microbiology and Epidemiology Beijing 100071China School of Basic Medicine Fourth Military Medical UniversityXi'an 710032China Jiangsu Pacific Meinuoke Biopharmceutical Co.Ltd Changzhou 213022China

SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing *** in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 ***,we identified CD147 as a universal receptor for SARS-CoV-2 and its ***,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,***,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and *** infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling ***,we proposed that severe COVID-19-related cytokine storm is induced by a"spike protein-CD147-CyPA signaling axis":Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK ***,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine ***,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its ***,our findings provided a new perspective for severe COVID-19-related ***,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment.

关键词： CD147 cytokine storm

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磷素积累导致马铃薯疮痂病加重及高效拮抗菌筛选

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Science Bulletin 2023年第20期68卷 2316-2320,M0004页

作者：曹晶晶汪志琴吴家和赵盼李成晨李小波刘璐赵永龙仲乃琴 State Key laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Guangdong Provincial Key laboratory of Crops Genetics and Improvement Crop Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China Engineering laboratory for Advanced Microbial Technology of Agriculture Chinese Academy of SciencesBeijing 100101China The Enterprise Key laboratory of Advanced Technology for Potato Fertilizer and Pesticide Hulunbuir 021000China

近年来,由致病性链霉菌(Streptomyces)引起的疮痂病在全世界各马铃薯产区普遍发生,危害程度逐年加重;病原菌土传和种传,难以防控,已成为威胁马铃薯产业可持续发展的重要瓶颈.本研究发现,磷肥施用量与疮痂病危害程度高度相关.磷素在土壤... 详细信息

近年来,由致病性链霉菌(Streptomyces)引起的疮痂病在全世界各马铃薯产区普遍发生,危害程度逐年加重;病原菌土传和种传,难以防控,已成为威胁马铃薯产业可持续发展的重要瓶颈.本研究发现,磷肥施用量与疮痂病危害程度高度相关.磷素在土壤中大量沉积不仅诱发致病性链霉菌的快速繁殖,也显著降低了拮抗菌的丰度和拮抗功能.平板和盆栽实验结果表明,疮痂链霉菌(***)对Ca_(3)(PO_(4))2吸收具有喜好性,基质中Ca_(3)(PO_(4))_(2)含量越高,病原菌繁殖速度越快,马铃薯块茎发病率和病情指数亦越高.代谢组学和X射线光电子能谱分析发现,疮痂链霉菌可通过分泌有机酸,将难溶性的Ca_(3)(PO_(4))_(2)转化成可溶性的HPO_(4)^(2-)供其生长繁殖所需,过量的H_(2)PO_(4)^(-)不利于其生存.实验证明,具有解磷功能的拮抗菌防控效果更好.我们筛选到一株具有溶磷功能的拮抗菌DX-9,可将Ca_(3)(PO_(4))_(2)转化成H2PO4-,有效降低土壤中Ca_(3)(PO_(4))_(2)的含量,对马铃薯疮痂病的田间防效可达70%.DX-9的规模化应用,将有望实现马铃薯疮痂病的有效防控.

关键词：马铃薯疮痂病繁殖速度病情指数磷肥施用量马铃薯块茎拮抗菌田间防效 X射线光电子能谱分析

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花生壳多酚提取物抗糖尿病活性的评价（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2018年第10期19卷 764-775页

作者： Xiao-meng SUN Hai-qing YE Jing-bo LIU Lei WU Ding-bo LIN Ya-li YU Feng GAO Department of food Science and Engineering College of Food Science and Engineering Jilin University Department of food Quality and Safety College of Food Science and Engineering Jilin University Department of Nutritional Sciences Oklahoma State University

目的:评估花生壳多酚提取物的抗糖尿病活性。创新点:明确了花生壳多酚的抗糖尿病活性。方法:通过高脂饮食/链脲霉素(HFD/STZ)诱导产生糖尿病小鼠。将糖尿病小鼠随机分为四组:二甲双胍组、花生壳多酚高、中、低剂量组(分别为200、100和50... 详细信息

目的:评估花生壳多酚提取物的抗糖尿病活性。创新点:明确了花生壳多酚的抗糖尿病活性。方法:通过高脂饮食/链脲霉素(HFD/STZ)诱导产生糖尿病小鼠。将糖尿病小鼠随机分为四组:二甲双胍组、花生壳多酚高、中、低剂量组(分别为200、100和50mg/kg);同时设置正常对照组及糖尿病对照组。二甲双胍及花生壳多酚每日口服一次,剂量为每天10 ml/kg体重,持续4周;考察不同剂量的花生壳多酚对糖尿病小鼠的体重、饮食、饮水,空腹血糖、血清总胆固醇、血脂、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇,及血液中超氧化物歧化酶、谷胱甘肽、丙二醛、肝糖原水平的影响;并对小鼠的肝脏、肾脏和胰岛组织进行组织学分析。结论:花生壳多酚具有一定的降血糖作用,可作为潜在的食物补充剂应用于糖尿病患者的饮食管理中。

关键词：花生壳多酚提取物抗糖尿病活性链脲佐菌素

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Effect of yellow rice wine on anti-aging ability in aged mice induced by D-galactose

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food Science and Human Wellness 2020年第2期9卷 184-191页

作者： Rong Liu Zukang Fu Fengjie Zhang Qingzhong Mao Chunguang Luan Xinglin Han Jie Xue Deliang Wang Si Qin Feike Hao College of food Science and Technology Hunan Agricultural UniversityChangsha410128China Kuaijishan Shaoxing Rice Wine CO.Ltd. Shaoxing312030China China National Research Institute of food and Fermentation Industries Beijing100015China

The present study is focused on investigation of the health protective effect of yellow rice wine(YRW)in mice and the potential underlying *** of aging were induced in mice by using Dgalactose(D-gal),and different doses of YRW(4,8,and 12 mL/kg BW)were orally administrated to D-galtreated mice for a period of six *** in vivo data obtained in the present study preliminary indicated that YRW markedly increased the activity of antioxidant enzymes and reduced the concentration of MDA both in the brain and ***,a forced swim test showed that moderate intake of YRW(8 mL/kg BW)significantly decreased the duration of immobility,reduced the blood content of urea nitrogen and lactic acid,and increased hepatic glycogen ***,results from the Morris water maze test suggested that YRW significantly reversed cognitive impairment and also alleviated neuroapoptosis in the experimental mice by regulating the gene expressions of Bax/Bcl-2 and *** above results indicated YRW has a potential anti-aging effect in mice and provided us certain molecular evidence for this action.

关键词： Yellow rice wine Aging Antioxidant Antifatigue Cognitive impairment Apoptosis

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Therapeutic potential of alkaloid extract from Codonopsis Radix in alleviating hepatic lipid accumulation: insights into mitochondrial energy metabolism and endoplasmic reticulum stress regulation in NAFLD mice

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Chinese Journal of Natural Medicines 2023年第6期21卷 411-422页

作者： FAN Cailian WANG Guan CHEN Miao LI Yao TANG Xiyang DAI Yi College of Medicine Henan Engineering Research Center of Funiu Mountain’s Medicinal Resources Utilization and Molecular MedicinePingdingshan UniversityPingdingshan 467000China Innovation Center of Nursing Research Nursing Key Laboratory of Sichuan ProvinceWest China HospitalSichuan University/West China School of NursingSichuan UniversityChengdu 610041China Guangdong Province Key laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research College of PharmacyJinan UniversityGuangzhou 510632China

Alkaloids are a class of naturally occurring bioactive compounds that are widely distributed in various food sources and Traditional Chinese *** study aimed to investigate the therapeutic effects and underlying mechanisms of alkaloid extract from Codonopsis Radix(ACR)in ameliorating hepatic lipid accumulation in a mouse model of non-alcoholic fatty liver disease(NAFLD)induced by a high-fat diet(HFD).The results revealed that ACR treatment effectively mitigated the abnormal weight gain and hepatic injury associated with ***,ACR ameliorated the dysregulated lipid metabolism in NAFLD mice,as evidenced by reductions in serum triglyceride,total cholesterol,and low-density lipoprotein levels,accompanied by a concomitant increase in the high-density lipoprotein *** treatment also demonstrated a profound anti-oxidative effect,effectively alleviating HFD-induced oxidative stress and promoting ATP *** effects were achieved through the up-regulation of the activities of mitochondrial electron transfer chain complexes Ⅰ,Ⅱ,Ⅳ,and Ⅴ,in addition to the activation of the AMPK/PGC-1α pathway,suggesting that ACR exhibits therapeutic potential in alleviating the HFD-induced dysregulation of mitochondrial energy ***,ACR administration mitigated HFD-induced endoplasmic reticulum(ER)stress and suppressed the overexpression of ubiquitin-specific protease 14(USP14)in NAFLD *** summary,the present study provides compelling evidence supporting the hepatoprotective role of ACR in alleviating lipid deposition in NAFLD by improving energy metabolism and reducing oxidative stress and ER *** findings warrant further investigation and merit the development of ACR as a potential therapeutic agent for NAFLD.

关键词： Codonopsis Radix Alkaloids Mitochondrial energy metabolism Endoplasmic Reticulum stress NAFLD

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Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomato

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Journal of Integrative Agriculture 2018年第5期17卷 1128-1136页

作者： Srinivasan Balamurugan Jayan Susan Ann Inchakalody P Varghese Shanmugaraj Bala Murugan Mani ChANDra Harish Sarma Rajeev Kumar Ramalingam Sathishkumar Plant Genetic Engineering laboratory Department of BiotechnologyBharathiar University

Antifreeze proteins（AFP） are produced by certain plants, animals, fungi and bacteria that enable them to survive upon extremely low temperature. Perennial rye grass, Lolium perenne, was reported to possess AFP which protects them from cold environments. In the present investigation, we isolated AFP gene from L. perenne and expressed it in tomato plants to elucidate its role upon chilling stress. The T1 transgenic tomato lines were selected and subjected to molecular, biochemical and physiological analyses. Stable integration and transcription of Lp AFP in transgenic tomato plants was confirmed by Southern blot hybridization and RT-PCR, respectively. Physiological analyses under chilling conditions showed that the chilling stress induced physiological damage in wild type（WT） plants, while the transgenic plants remained healthy. Total sugar content increased gradually in both WT and transgenic plants throughout the chilling treatment. Interestingly, transgenic plants exhibited remarkable alterations in terms of relative water content（RWC） and electrolyte leakage index（ELI） than those of WT. RWC increased significantly by 3-fold and the electrolyte leakage was reduced by 2.6-fold in transgenic plants comparing with WT. Overall, this report proved that Lp AFP gene confers chilling tolerance in transgenic tomato plants and it could be a potential candidate to extrapolate the chilling tolerance on other chilling-sensitive food crops.

关键词： Lolium perenne antifreeze protein chilling tolerance genetic transformation transgenic tomato

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Structure tailorable triple-phase and pure double-polar-phase flexible IF-WS_(2)@poly(vinylidene fluoride)nanocomposites with enhanced electrical and mechanical properties

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Journal of Materiomics 2020年第3期6卷 563-572页

作者： Dong Guo Kai Cai Pingye Deng Guoning Si Liangying Sun Fahui Chen Honglong Ning Li Jin Jingtao Ma School of Medical Instrument&food Engineering University of Shanghai for Science&TechnologyShanghai200093China School of Materials Science&Engineering Beihang UniversityBeijing100191China Beijing Center for Physical and Chemical Analysis Beijing100089China Institute of Polymer Optoelectronic Materials and Devices State Key Laboratory of Luminescent Materials and DevicesSouth China University of TechnologyGuangzhou510640China Electronic Materials Research laboratory School of Electronic and Information EngineeringXi’an Jiaotong UniversityXi’an710049China State Key laboratory of New Ceramics and Fine Processing Tsinghua UniversityBeijing100084China

A new low-cost high-permittivity flexible nanocomposite consisting of a polyvinylidene fluoride(PVDF)matrix and fullerene-like tungsten disulfide nanoparticle(IF-WS2 NP)filler was fabricated via a simple solution route.A comprehensive investigation by X-ray diffraction,attenuated total reflection-infrared spectroscopy,and differential scanning calorimetry(DSC)showed that 0.5e1 vol% of IF-WS_(2) induced a nonpolar α-phase to coexisting triple-phase transition in PVDF,whereas a slightly higher loading of 2 vol% induced pure double-polar β- and γ-*** results indicate that the structure and properties of the fabricated nanocomposite are easily *** during heating and cooling cycles and morphology observations further indicated that a polar phase was induced by the nucleation effect of the IF-WS2 NPs and electrostatic *** a consequence of the multiphase coexistence and structure homogeneity,nanocomposites with approximately 0.5-1 vol% of IF-WS_(2) NPs showed enhanced dielectric and energy-storage performance as well as enhanced tensile strength and *** new phase-tailorable nanocomposites with balanced properties are promising for applications as energystorage capacitors,piezoelectric sensors,and other flexible multifunctional *** results of this study will serve to deepen the understanding of the polymer polymorph and provide directions on new routes for fabrication of smart materials.

关键词： PVDF Polymorph Permittivity Piezoelectricity Flexibility

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Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis

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Chinese Herbal Medicines 2022年第4期14卷 554-562,F0002页

作者： Xingyan Zhang Xiaoyan Lian Huling Li Wenjing Zhao Xin Li Fujun Zhou Yutong Zhou Tao Cui Yuli Wang Changxiao Liu Key laboratory of Quality-marker of Traditional Chinese Medicines Tianjin Institute of Pharmaceutical Research Co.Ltd.Tianjin 300462China Center for Drug Evaluation National Medical Products AdministrationBeijing 100022China Department of Pharmacology Tianjin Medical UniversityTianjin 300070China State Key laboratory of Drug Delivery Technology and Pharmacokinetics Tianjin Institute of Pharmaceutical Research Co.Ltd.Tianjin 300462China Key laboratory of Systems Bioengineering(Ministry of Education) School of Chemistry Engineering and TechnologyTianjin UniversityTianjin 300072China Research Unit for Drug Metabolism Chinese Academy of Medical SciencesBeijing 100730China Pharmaceuticai Armaceuticai DA REN TANG Group Corporation Limited Traditional Chinese Pharmacy Researchinstitute Tianjin 300457China Tianjin Key laboratory of Quality Control in Chinese Medicine Tianjin 300457China

Objective:Taxifolin is a natural flavonoid compound that can be isolated from onions,grapes,oranges and *** also acts as a medicine food homology with extraordinary antioxidant and antiinflammatory *** study aims to explain the protective effects and potential mechanisms of taxifolin against inflammatory ***:Levels of interleukin(IL)-6,IL-1βand intracellular reactive oxygen species(ROS)were assessed in different time after the treatment of taxifolin in RAW264.7 cells induced by lipopolysaccharide(LPS).Subsequently,the m RNA and protein levels of inducible nitric oxide synthase(i NOS),vascular endothelial growth factor(VEGF),cyclooxygenase(COX)-2,tumor necrosis factor(TNF)-a and the phosphorylation expression levels of the MAPK signal pathway were also evaluated.A silico analysis was used to explain the binding situation for the investigation of taxifolin and MAPK signal *** then MAPK inhibitors were used to reveal the expression level of i NOS,VEGF,COX-2 and TNF-a in RAW264.7 ***:It was demonstrated that cell inflammatory damage induced by LPS was significantly alleviated after the treatment of ***,the m RNA and protein levels of i NOS,VEGF,COX-2 and TNF-a were reduced and the phosphorylation expression levels of the MAPK signal pathway were down-regulated remarkably as *** silico analysis,taxifolin could form a relatively stable combination with MAPK signal *** inhibitors showed increasing or decreasing effect in the m RNA levels of i NOS,VEGF,COX-2 and TNF-a,which suggesting that taxifolin down-regulated i NOS,VEGF,COX-2 and TNF-a expressions were not entirely through the MAPK ***:This finding demonstrated that taxifolin improved the inflammatory responses that partly involved in the phosphorylation expression level of MAPK signal pathway in RAW264.7 cells exposed to acute stress.

关键词： MAPK signal pathway molecular docking pro-inflammatory cytokines taxifolin

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Derivation and applications of human hepatocyte-like cells

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World Journal of Stem Cells 2019年第8期11卷 535-547页

作者： Shuang Li Shi-Qian Huang Yong-Xu Zhao Yu-Jie Ding Dan-Jun Ma Qiu-Rong Ding CAS Key laboratory of Nutrition Metabolism and Food Safety Shanghai Institute of Nutrition and Health Shanghai Institutes for Biological Sciences University of Chinese Academy of Sciences Chinese Academy of Sciences Shanghai 200031 China College of Mechanical Engineering Dongguan University of Technology Dongguan 523808 Guangdong Province China Department of Pharmacy Mudanjiang Kang’an Hospital Mudanjiang 157011 Heilongjiang Province China Institute for Stem Cell and Regeneration Chinese Academy of Sciences Beijing 100101 China

Human hepatocyte-like cells (HLCs) derived from human pluripotent stem cells (hPSCs) promise a valuable source of cells with human genetic background, physiologically relevant liver functions, and unlimited supply. With over 10 years’ efforts in this field, great achievements have been made. HLCs have been successfully derived and applied in disease modeling, toxicity testing and drug discovery. Large cohorts of induced pluripotent stem cells-derived HLCs have been recently applied in studying population genetics and functional outputs of common genetic variants in vitro. This has offered a new paradigm for genomewide association studies and possibly in vitro pharmacogenomics in the nearly future. However, HLCs have not yet been successfully applied in bioartificial liver devices and have only displayed limited success in cell transplantation. HLCs still have an immature hepatocyte phenotype and exist as a population with great heterogeneity, and HLCs derived from different hPSC lines display variable differentiation efficiency. Therefore, continuous improvement to the quality of HLCs, deeper investigation of relevant biological processes, and proper adaptation of recent advances in cell culture platforms, genome editing technology, and bioengineering systems are required before HLCs can fulfill the needs in basic and translational research. In this review, we summarize the discoveries, achievements, and challenges in the derivation and applications of HLCs.

关键词： Hepatocyte-like cells Human pluripotent stem cells Hepatic differentiation Biomedical application

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