Reproductive toxicity of enrofloxacin in Caenorhabditis elegans involves oxidative stress-induced cell apoptosis

作     者：Jiahao Huang Lizi Liao Guowei Wang Zhongkun Du Zhengxing Wu Jiahao Huang;Lizi Liao;Guowei Wang;Zhongkun Du;Zhengxing Wu

作者机构：Key Laboratory of Molecular BiophysicsMinistry of EducationCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China School of Environmental Ecology and Biological EngineeringWuhan Institute of TechnologyWuhan 430205China Key Laboratory of Green Chemical Engineering Process of Ministry of EducationWuhan 430205China College of Resources and EnvironmentShandong Agricultural UniversityTaian 271018China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2023年第127卷第5期

页      面：726-737页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China(No.21806047) the National Postdoctoral Program for Innovative Talents of China(No.BX201700310) the Postdoctoral Science Foundation of China(No.2018M632869) the 16th University President Fund of Wuhan University of Technology(No.XZJJ2021105) 

主　　题：Environmental toxicity Nematodes Chronic exposure Oxidative stress response Germ cell apoptosis 

摘      要：Fluoroquinolone antibiotics(FQs)that persist and bioaccumulate in the environment have aroused people’s great ***,we studied the adverse effects of FQs in soil animals of Caenorhabditis elegans via food-chronically *** result shows *** exposed to FQs exhibited reproductive toxicity with small-brood size and low-egg *** study the underlying mechanism,we conduct a deep investigation of enrofloxacin(ENR),one of the most frequently detected FQs,on nematodes which is one of commonly used animal indicator of soil *** concentration-effect curves simulated by the Hill model showed that the half effect concentrations(EC50)of ENR were(494.3±272.9)μmol/kg and(107.4±30.9)μmol/kg for the brood size and the hatchability,*** gene expression between the control and the ENR-exposure group enriched with the oxidative stress and cell apoptosis *** results together with the enzyme activity in oxidative stress and the cell corpses suggested that ENR-induced reproductive toxicity was related to germ cell apoptosis under oxidative *** risk quotients of some soil and livestock samples were calculated based on the threshold value of EC10 for the egg hatchability(2.65μmol/kg).The results indicated that there was possible reproductive toxicity on the nematodes in certain agricultural soils for the *** study suggested that chronic exposure to FQs at certain levels in environment would induce reproductive toxicity to the nematodes and might reduce the soil sustainability,alarming the environment risks of antibiotics abuse.