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Biotinylated polyurethane-urea nanoparticles for targeted theranostics in human hepatocellular carcinoma

Nano Research 2015年第5期8卷 1729-1745页

作者： Genoveva MorraI-Ruiz Pedro Melgar-Lesmes Andrea Lopez-Vicente Conxita Solans Maria Jose Garcia-Celma Department of Pharmacy and Pharmaceutical Technology Faculty of Pharmacy University of Barcelona Av Joan XXIII s/n 08028 Barcelona Spain Institute of Advanced Chemistry of Catalonia (IQAC) CS/C Jordi Girona 18-26 08034 Barcelona Spain Networking Research Center on bioengineering Biomaterials and Nanomedicine CIBER-BBN Spain

Over the past years, significant efforts have been devoted to explore novel drug delivery and detection strategies for simultaneous therapy and diagnostics. The development of biotinylated polyurethane-urea nanoparticles as theranostic nanocarriers for targeted drug and plasmid delivery, for fluorescence detection of human hepatocellular carcinoma cells, is described herein. These targeted nanoparticles are specifically designed to incorporate biotin into the polymeric matrix, since many tumor types overexpress receptors for biotin as a mechanism to boost uncontrolled cell growth. The obtained nanoparticles were spherical, exhibited an average diameter ranging 110-145 nm, and showed no cytotoxicity in healthy endothelial cells. Biotinylated nanoparticles are selectively incorporated into the perinuclear and nuclear area of the human hepatocellular carcinoma cell line, HepG2, in division, but not into growing, healthy, human endothelial cells. Indeed, the simultaneous incorporation of the anticancer drugs, phenoxodiol or sunitinib, together with plasmid DNA encoding green fluorescent protein, into these nanoparticles allows a targeted pharmacological antitumor effect and furthermore, selective transfection of a reporter gene, to detect these cancer cells. The combined targeted therapy and detection strategy described here could be exploited for liver cancer therapy and diagnostics, with a moderate safety profile, and may also be a potential tool for other types of cancer.

关键词： cancer therapy DNA nanoparticles polyurethane theranostics

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Boric Acid-Based Dual Modulation Photoluminescent Glucose Sensor Using Thioglycolic Acid-Capped CdTe Quantum Dots

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Journal of Analysis and Testing 2017年第4期1卷 291-297页

作者： Saadat Majeed WenyueGao Jianping Lai Chao Wang JianpingLi Zhongyuan Liu Guobao Xu State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of Sciences5625 Renmin StreetChangchun 130022JilinPeople’s Republic of China University of Chinese Academy of Sciences Chinese Academy of SciencesNo.19A YuquanluBeijing 100049China division of Analytical Chemistry Institute of Chemical SciencesBahauddin Zakariya UniversityMultan 60800Pakistan College of Chemistry and bioengineering Guilin University of TechnologyGuilin 541004People’s Republic of China

Most luminescent glucose sensors based on the interaction of glucose with organic boric acids or ***,a new luminescent glucose sensor is designed using thioglycolic acid-capped CdTe quantum dots in the presence of cheap inorganic boric *** peak position and intensities change upon the addition of glucose because of the interaction of boric acid with glucose and thioglycolic acid-capped CdTe quantum dots,which enables glucose detection by either color change or intensity *** luminescent intensities change linearly with glucose concentrations in the ranges from 0.03 to 1 mM and 1–25 mM with a detection limit of 10μM(S/N=3).Moreover,glucose concentrations can be conveniently detected by color change in the range from 1 mM–25 *** displays a highly selective response to glucose over other interfering but biologically important saccharides,amino acids,and common *** Abstract A thioglycolic acid-capped CdTe QD-based sensor can detect glucose with wide linear range by change in intensity or color in the presence of cheap inorganic boric acid.

关键词： CdTe quantum dots Glucose sensor Thioglycolic acid

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Wide-field ophthalmic space-division multiplexing optical coherence tomography

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Photonics Research 2020年第4期8卷 539-547页

作者： JASON JERWICK YONGYANG HUANG ZHAO DONG ADRIENNE SLAUDADES ALEXANDER J.BRUCKER CHAO ZHOU Department of Electrical and Computer Engineering Lehigh UniversityBethlehemPennsylvania 18015USA Department of Biomedical Engineering Washington University in St.LouisSt.LouisMissouri 63130USA Scheie Eye Institute Penn Presbyterian Medical CenterUniversity of PennsylvaniaPhiladelphiaPennsylvania 19104USA Department of bioengineering Lehigh UniversityBethlehemPennsylvania 18015USA

High-speed ophthalmic optical coherence tomography(OCT)systems are of interest because they allow rapid,motion-free,and wide-field retinal ***-division multiplexing optical coherence tomography(SDMOCT)is a high-speed imaging technology that takes advantage of the long coherence length of micro electro mechanical vertical cavity surface emitting laser sources to multiplex multiple images along a single imaging *** demonstrate wide-field retinal OCT imaging,acquired at an effective A-scan rate of 800,000 A-scans/s with volumetric images covering up to 12.5 mm×7.4 mm on the retina and captured in less than 1 s.A clinical feasibility study was conducted to compare the ophthalmic SDM-OCT with commercial OCT systems,illustrating the high-speed capability of SDM-OCT in a clinical setting.

关键词： Wide-field ophthalmic space-division multiplexing optical coherence tomography

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GIGANTEA and EARLY FLOWERING 4 in Arabidopsis Exhibit Differential Phase-Specific Genetic Influences over a Diurnal Cycle

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Molecular Plant 2012年第3期5卷 678-687页

作者： Yumi Kim Miji Yeom Hyunmin Kim Junhyun Lim Hee Jung Koo Daehee Hwang David Somers Hong Gil Nam division of Molecular and Life Sciences POSTECH Hyojadong Pohang Kyungbuk 790-784 Republic of Korea School of Interdisciplinary Bioscience and bioengineering POSTECH Hyojadong Pohang Kyungbuk 790-784 Republic of Korea Department of Chemical Engineering POSTECH Hyojadong Pohang Kyungbuk 790-784 Republic of Korea Integrative Biosciences and Biotechnology POSTECH Hyojadong Pohang Kyungbuk 790-784 Republic of Korea Department of Molecular Genetics The Ohio State University 244B Rightmire Hall 1060 Carmack Road Columbus OH 43210 USA National Core Research Center for Systems Bio-Dynamics POSTECH Hyojadong Pohang Kyungbuk 790-784 Republic of Korea

The endogenous circadian clock regulates many physiological processes related to plant survival and adapt- ability. GIGANTEA （GI）, a clock-associated protein, contributes to the maintenance of circadian period length and ampli- tude, and also regulates flowering time and hypocotyl growth in response to day length. Similarly, EARLY FLOWERING 4 （ELF4）, another clock regulator, also contributes to these processes. However, little is known about either the genetic or molecular interactions between GI and ELF4 in Arabidopsis. In this study, we investigated the genetic interactions between GI and ELF4 in the regulation of circadian clock-controlled outputs. Our mutant analysis shows that GI is epistatic to ELF4 in flowering time determination, while ELF4 is epistatic to GI in hypocotyl growth regulation. Moreover, GI and ELF4 have a synergistic or additive effect on endogenous clock regulation. Gene expression profiling of gi, elf4, and gi elf4 mutants further established that GI and ELF4 have differentially dominant influences on circadian physiological outputs at dusk and dawn, respectively. This phasing of GI and ELF4 influences provides a potential means to achieve diversity in the regulation of circadian physiological outputs, including flowering time and hypocotyl growth.

关键词： microarray LHY endogenous clock GI ELF4

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Critical factors to inhibit water-splitting side reaction in carbon-based electrode materials for zinc metal anodes

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Carbon Energy 2022年第6期4卷 1080-1092页

作者： Dong Hyuk Kang Eunji Lee Beom Sik Youn Son Ha Jong Chan Hyun Juhee Yoon Dawon Jang Kyoung Sun Kim Hyungsub Kim Sang Moon Lee Sungho Lee Hyoung-Joon Jin Hyung-Kyu Lim Young Soo Yun KU-KIST Graduate School of Converging Science and Technology Korea UniversitySeoulSeongbuk-guSouth Korea division of Chemical Engineering and bioengineering Kangwon National UniversityChuncheonGangwon-doSouth Korea Department of Eco-Polymer Science and Engineering Inha UniversityIncheonMichuhol-guSouth Korea Carbon Composite Materials Research Center Institute of Advanced Composite MaterialsKorea Institute of Science and Technology(KIST)Jeollabuk-doSouth Korea Department of Chemical and Biological Engineering Korea UniversitySeoulSeongbuk-guSouth Korea Neutron Science division Korea Atomic Energy Research Institute(KAERI)DaejeonYuseong-guSouth Korea Research Center for Materials Analysis Korea Basic Science Institute(KBSI)DaejeonYuseong-guSouth Korea Department of Integrative Energy Engineering Korea UniversitySeoulSeongbuk-guSouth Korea

Zinc metal anodes(ZMA)have high theoretical capacities(820 mAh g−1 and 5855 mAh cm−3)and redox potential(−0.76 V *** hydrogen electrode),similar to the electrochemical voltage window of the hydrogen evolution reaction(HER)in a mild acidic electrolyte system,facilitating aqueous zinc batteries competitive in next-generation energy storage ***,the HER and byproduct formation effectuated by water-splitting deteriorate the electrochemical performance of ZMA,limiting their *** this study,a key factor in promoting the HER in carbon-based electrode materials(CEMs),which can provide a larger active surface area and guide uniform zinc metal deposition,was investigated using a series of threedimensional structured templating carbon electrodes(3D-TCEs)with different local graphitic orderings,pore structures,and surface *** ultramicropores of CEMs are the determining critical factors in initiating HER and clogging active surfaces by Zn(OH)2 byproduct formation,through a systematic comparative study based on the 3D-TCE series *** the 3D-TCEs had a proper graphitic structure with few ultramicropores,they showed highly stable cycling performances over 2000 cycles with average Coulombic efficiencies of≥99%.These results suggest that a well-designed CEM can lead to high-performance ZMA in aqueous zinc batteries.

关键词： aqueous batteries carbon electrode hydrogen evolution reaction multivalent ion zinc metal anode

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Opportunities with NHC Organocatalysis: Toward New Activation Modes

Opportunities with NHC Organocatalysis: Toward New Activatio...

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中国化学会第九届全国有机化学学术会议

作者：池永贵 International Joint Laboratory for Chiral Synthesis and Green Pesticides Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of Education Guizhou University division of Chemistry and Biological Chemistry School of Physical and Mathematical Sciences Nanyang Technological University

＜正＞The Chi group works on the discovery and development of sustainable organic catalysis and new synthetic strategies for organic synthesis,pharmaceutical manufacturing,and materials assemblies or *** focus is to develop basic new action modes for the functionalization of relatively inert C-H,C-C,N-S,and other non-reactive chemical bonds by using N-heterocyclic carbene（NHC）organic catalysts or through the combination of multiple catalysts.

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Investigating the consequences of asymmetric endoplasmic reticulum inheritance in Saccharomyces cerevisiae under stress using a combination of single cell measurements and mathematical modelling

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Synthetic and Systems Biotechnology 2018年第1期3卷 64-75页

作者： Felix R.H.Jonas Kate E.Royle Rochelle Aw Guy-Bart V.Stan Karen M.Polizzi Department of bioengineering Imperial College LondonLondonSW72AZUnited Kingdom Imperial College Centre for Synthetic Biology LondonSW72AZUnited Kingdom Department of Life Sciences Imperial College LondonLondonSW72AZUnited Kingdom

Adaptation allows organisms to maintain a constant internal environment,which is optimised for *** unfolded protein response(UPR)is an example of a feedback loop that maintains endoplasmic reticulum(ER)homeostasis,and is characteristic of how adaptation is often mediated by transcriptional *** more recent discovery of asymmetric division in maintaining ER homeostasis,however,is an example of how alternative non-transcriptional pathways can exist,but are overlooked by gold standard transcriptomic or proteomic population-based *** this study,we have used a combination of fluorescent reporters,flow cytometry and mathematical modelling to explore the relative roles of asymmetric cell division and the UPR in maintaining ER *** low ER stress,asymmetric division leaves daughter cells with an ER deficiency,necessitating activation of the UPR and prolonged cell cycle during which they can recover ER functionality before *** analysis of and simulation results from our mathematical model reinforce the experimental observations that low ER stress primarily impacts the growth rate of the daughter *** results demonstrate the interplay between homeostatic pathways and the importance of exploring sub-population dynamics to understand population adaptation to quantitatively different stresses.

关键词： Adaptation Asymmetric cell division Endoplasmic reticulum stress Unfolded protein response Saccharomyces cerevisiae

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Measurement of copy number variation in single cancer cells using rapid-emulsification digital droplet MDA

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Microsystems & Nanoengineering 2017年第1期3卷 260-266页

作者： Samuel C.Kim Gayatri Premasekharan Iain C.Clark Hawi B.Gemeda Pamela L.Paris Adam R.Abate Department of bioengineering and Therapeutic Sciences University of CaliforniaSan Francisco(UCSF)California Institute for Quantitative Biosciences(QB3)San FranciscoSan FranciscoCA 94158USA Department of Urology Division of Hematology&OncologyUniversity of CaliforniaSan Francisco(UCSF)San FranciscoCA 94158USA

Uniform amplification of low-input DNA is important for applications across biology,including single-cell genomics,forensic science,and microbial and viral ***,the requisite biochemical amplification methods are prone to bias,skewing sequence proportions and obscuring signals relating to copy *** droplet multiple displacement amplification enables uniform amplification but requires expert knowledge of microfluidics to generate monodisperse *** addition,existing microfluidic methods are tedious and labor intensive for preparing many ***,we introduce rapid-emulsification multiple displacement amplification,a method to generate monodisperse droplets with a hand-held syringe and hierarchical droplet *** conventional microfluidic devices require >10 min to emulsify a sample,our system requires tens of seconds and yields data of equivalent *** demonstrate the approach by using it to accurately measure copy number variation(CNV)in single cancer cells.

关键词： amplification bias copy number variation ddMDA droplet microfluidics multiple displacement amplification

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Relative bioavailability of tribasic zinc sulfate for broilers fed a conventional corn-soybean meal diet

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Journal of Integrative Agriculture 2015年第10期14卷 2042-2049页

作者： LI Wen-xiang MA Xin-yan LU Lin ZHANG Li-yang LUO Xu-gang Mineral Nutrition Research division Institute of Animal Sciences Chinese Academy of Agricultural Sciences Institute of Animal Science Guangdong Academy of Agricultural Sciences

An experiment was carried out to investigate the relative bioavailability of tribasic zinc（Zn） sulfate relative to Zn sulfate for broilers fed a conventional corn-soybean meal diet. A total of 504 1-d-old Arbor Acres commercial male chicks were randomly assigned by body weight of birds to one of seven treatments involving a 2×3 factorial arrangement with three levels of added Zn（30, 60, or 90 mg of Zn kg–1） and two Zn sources（tribasic Zn sulfate and Zn sulfate） plus a control with no added Zn for an experimental phase of 14 d. Plasma Zn, tibia ash Zn, pancreas Zn, and pancreas metallothionein（MT） messenger RNA（m RNA） were analyzed at 6 or 14 d of age post-hatching. The results showed that plasma Zn, tibia ash Zn, pancreas Zn, and pancreas MT m RNA increased linearly（P〈0.002） as dietary Zn concentration increased at 6 or 14 d of age. The R2 for a linear model was greater on d 6 than on d 14 for the above 4 responsive criteria, and among these indices, the fitting of the tibia ash Zn concentration was the best（R2=0.99）. Therefore, based on slope ratios from the multiple linear regressions of the above 4 indices of the birds at 6 d of age on daily intake of dietary Zn, the bioavailabilities of tribasic Zn sulfate relative to Zn sulfate（100%） were 95.6%（P=0.18）, 83.5%（P=0.01）, 87.9%（P=0.53）, and 75.9%（P=0.38） for the tibia ash Zn, pancreas Zn, plasma Zn, and pancreas MT m RNA, respectively. The results indicated that generally, Zn from tribasic Zn sulfate was as available as Zn from Zn sulfate for broilers.

关键词： tribasic zinc sulfate bioavailability Zn concentration MT gene expression broilers

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Synthetic aperture-based on-chip microscopy

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Light(Science & Applications) 2015年第1期4卷 438-446页

作者： Wei Luo Alon Greenbaum Yibo Zhang Aydogan Ozcan Electrical Engineering Department University of CaliforniaLos AngelesCA 90095USA bioengineering Department University of CaliforniaLos AngelesCA 90095USA California NanoSystems Institute(CNSI) University of CaliforniaLos AngelesCA 90095USA Department of Surgery David Geffen School of MedicineUniversity of CaliforniaLos AngelesCA 90095USA

Wide field-of-view(FOV)and high-resolution imaging requires microscopy modalities to have large space-bandwidth *** on-chip microscopy decouples resolution from FOV and can achieve a space-bandwidth product greater than one billion under unit magnification using state-of-the-art opto-electronic sensor chips and pixel super-resolution ***,using vertical illumination,the effective numerical aperture(NA)that can be achieved with an on-chip microscope is limited by a poor signal-to-noise ratio(SNR)at high spatial frequencies and imaging artifacts that arise as a result of the relatively narrow acceptance angles of the sensor’s ***,we report,for the first time,a synthetic aperture-based on-chip microscope in which the illumination angle is scanned across the surface of a dome to increase the effective NA of the reconstructed lensfree image to 1.4,achieving e.g.,,250-nm resolution at 700-nm wavelength under unit *** synthetic aperture approach not only represents the largest NA achieved to date using an on-chip microscope but also enables color imaging of connected tissue samples,such as pathology slides,by achieving robust phase recovery without the need for multi-height scanning or any prior information about the *** validate the effectiveness of this synthetic aperture-based,partially coherent,holographic on-chip microscope,we have successfully imaged color-stained cancer tissue slides as well as unstained Papanicolaou smears across a very large FOV of 20.5 mm^(2).This compact on-chip microscope based on a synthetic aperture approach could be useful for various applications in medicine,physical sciences and engineering that demand high-resolution wide-field imaging.

关键词： computational imaging lensfree microscopy on-chip microscopy synthetic aperture

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