Investigating the consequences of asymmetric endoplasmic reticulum inheritance in Saccharomyces cerevisiae under stress using a combination of single cell measurements and mathematical modelling
作者机构：Department of BioengineeringImperial College LondonLondonSW72AZUnited Kingdom Imperial College Centre for Synthetic BiologyLondonSW72AZUnited Kingdom Department of Life SciencesImperial College LondonLondonSW72AZUnited Kingdom
出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)
年 卷 期：2018年第3卷第1期
页 面：64-75页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 081702[工学-化学工艺] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 0703[理学-化学] 0836[工学-生物工程]
基 金：ESPRC, (GBS EP/K020617/1) Engineering and Physical Sciences Research Council, EPSRC, (EP/K020617/1, EP/K038648/1, EP/M002187/1)
主 题：Adaptation Asymmetric cell division Endoplasmic reticulum stress Unfolded protein response Saccharomyces cerevisiae
摘 要：Adaptation allows organisms to maintain a constant internal environment,which is optimised for *** unfolded protein response(UPR)is an example of a feedback loop that maintains endoplasmic reticulum(ER)homeostasis,and is characteristic of how adaptation is often mediated by transcriptional *** more recent discovery of asymmetric division in maintaining ER homeostasis,however,is an example of how alternative non-transcriptional pathways can exist,but are overlooked by gold standard transcriptomic or proteomic population-based *** this study,we have used a combination of fluorescent reporters,flow cytometry and mathematical modelling to explore the relative roles of asymmetric cell division and the UPR in maintaining ER *** low ER stress,asymmetric division leaves daughter cells with an ER deficiency,necessitating activation of the UPR and prolonged cell cycle during which they can recover ER functionality before *** analysis of and simulation results from our mathematical model reinforce the experimental observations that low ER stress primarily impacts the growth rate of the daughter *** results demonstrate the interplay between homeostatic pathways and the importance of exploring sub-population dynamics to understand population adaptation to quantitatively different stresses.
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