检索结果-南通市图书馆

BMPRⅡ^(+)neural precursor cells isolated and characterized from organotypic neurospheres:an in vitro model of human fetal spinal cord development

引用

Neural Regeneration Research 2024年第2期19卷 447-457页

作者： Michael W.Weible II Michael D.Lovelace Hamish D.Mundell Tsz Wai Rosita Pang Tailoi Chan-Ling Bosch Institute Discipline of Anatomy and Histology(F13)University of SydneySydneyNSWAustralia School of Environment and Science Griffith UniversityNathanQLDAustralia discipline of Medicine Nepean Clinical SchoolFaculty of Medicine and HealthUniversity of SydneyKingswoodNSWAustralia New South Wales Brain Tissue Resource Centre School of Medical SciencesFaculty of Medicine and HealthUniversity of SydneyCharles Perkins Centre(D17)SydneyNSWAustralia

Roof plate secretion of bone morphogenetic proteins(BMPs)directs the cellular fate of sensory neurons during spinal cord development,including the formation of the ascending sensory columns,though their biology is not well ***-ⅡBMP receptor(BMPRⅡ),the cognate receptor,is expressed by neural precursor cells during embryogenesis;however,an in vitro method of enriching BMPRⅡ^(+)human neural precursor cells(hNPCs)from the fetal spinal cord is *** was undertaken on intact second-trimester human fetal spinal cord using antibodies to BMPRⅡand leukemia inhibitory factor(LIF).Regions of highest BMPRⅡ^(+)immunofluorescence localized to sensory *** and meningeal-associated BMPRⅡ^(+)vascular cells were identified in both intact fetal spinal cord and cortex by co-positivity with vascular lineage markers,CD34/*** immunostaining identified a population of somas concentrated in dorsal and ventral horn interneurons,mirroring the expression of LIF receptor/CD118.A combination of LIF supplementation and high-density culture maintained culture growth beyond 10 passages,while synergistically increasing the proportion of neurospheres with a stratified,*** neurospheres were characterized by BMPRⅡ^(+)/MAP2ab^(+/–)/βⅢ-tubulin^(+)/nestin^(–)/vimentin^(–)/GFAP^(–)/NeuN^(–)surface hNPCs surrounding a heterogeneous core ofβⅢ-tubulin^(+)/nestin^(+)/vimentin^(+)/GFAP^(+)/MAP2ab^(–)/NeuN^(–)multipotent *** cultures from tripotential neurospheres contained neuronal(βⅢ-tubulin^(+)),astrocytic(GFAP+),and oligodendrocytic(O4+)lineage ***-activated cell sorting-sorted BMPRⅡ^(+)hNPCs were MAP2ab^(+/–)/βⅢ-tubulin^(+)/GFAP^(–)/O4^(–)in *** is the first isolation of BMPRⅡ^(+)hNPCs identified and characterized in human fetal spinal *** data show that LIF combines synergistically with high-density reaggregate cultures to support the organotypic reorganization of neurospheres,characterize

关键词： BMPRⅡ bone morphogenetic protein histotypic human spinal cord development leukemia inhibitory factor neurosphere organotypic reaggregate sensory columns

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Intracranial pressure is elevated at 24h post-stroke inmice

引用

Neuroprotection 2024年第1期2卷 60-64页

作者： Kirby E.Warren Rebecca J.Hood Fredrick R.Walker Neil J.Spratt School of Biomedical Sciences and Pharmacy University of NewcastleCallaghanAustralia Hunter Medical Research Institute NewLambton HeightsAustralia School of Biomedicine Discipline of Anatomy and PathologyFaculty of Health and Medical SciencesThe University of AdelaideAdelaideAustralia Department of Neurology John Hunter HospitalNewLambton HeightsAustralia

Background:It has long been assumed that post-stroke intracranial pressure(ICP)elevation occurs because of large infarct and edema ***,we have repeatedly shown IcP elevation at 24 h post-stroke in the presence of little to no edema in *** processes are often conserved across species and types of ***,we aimed to determine if an ICP rise occurs at 24 h post-stroke in the presence of small infarct and edema volumes in ***:Mice were randomized by random number table to either photothrombotic stroke or sham surgery(n=15).Epidural ICP was recorded using a fiber optic catheter at 1 h post-stroke(baseline)and between 23 and ***:ICP was significantly higher at 24 h compared to baseline in stroke animals(n=6;10.71±6.45mmHg vs.3.74±2.20mmHg,respec-tively;p=0.03).ICP at 24 h was also significantly higher in stroke mice compared to sham(n=6;3.45±1.43mmHg;p=0.02).There was no change in ICP in sham mice(p=0.9).Edema volumes in stroke animals were small(0.04±0.04 mm3)and unlikely to have caused significant ICP ***:This study provides evidence of an edema-independent ICP elevation following small ischemic stroke in *** occurrence of this rise supports our findings in other species and suggests it is caused by a previously undescribed mechanism.

关键词： cerebraledema intracranial pressure ischemic stroke

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mechanical sensor PDLIM5 promotes the osteogenesis of human adipose-derived stem cells through microfilament alterations

引用

Genes & Diseases 2024年第3期11卷 136-139页

作者： Yuchao Yang Shutong Wu Rongmei Qu Congrong Wang Jinyang Wang Asmat Ullah Khan You Pan Wenqing Liu Jinhui Zhu Muhammad Akram Khan Chujiang Xu Jingxing Dai Jun Ouyang Guangdong Provincial Key Laboratory of Medical Biomechanics&National Key discipline of Human anatomy Schoolof BasicMedical SciencesSouthern Medical UniversityGuangzhouGuangdong 510515China Department of Laboratory Medicine Nanfang Hospital of Southern Medical UniversityGuangzhouGuangdong 510515China Department of Pathology Guangdong Provincial People's HospitalGuangdong Academy of Medical SciencesGuangzhouGuangdong 510080China Department of Breast Surgery Guangxi Medical University Cancer HospitalNanningGuangxi 530000China Department of Veterinary Pathology Faculty of Veterinary and Animal SciencesPMAS Arid Agriculture UniversityRawalpindi 46300Pakistan Department of Orthopedics TCM-Integrated HospitalSouthern Medical UniversityGuangzhouGuangdong 510000China

Bone regeneration is a multifaceted,abstract,and wellcoordinated physiological progression of bone formation that participates in continuous regeneration and remodeling throughout ***,when it comes to complex clinical situations requiring extensive bone regeneration,such as massive bone defects caused by injuries,infection,or tumor removal,traditional methods do not often yield good treatment strategies or protocols due to their limitations.

关键词： alterations injuries massive

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Reproductive biology of an old endemic murid rodent of Australia, the Spinifex hopping mouse, Notomys alexis:adaptations for life in the arid zone

引用

Integrative Zoology 2011年第4期6卷 321-333页

作者： William G.BREED Chris M.LEIGH discipline of anatomy and Pathology University of AdelaideAdelaideAustralia

The Spinifex hopping mouse(Notomys alexis Thomas,1922)is an arid adapted Australo–Papuan old endemic rodent that undergoes boom and bust population *** this communication,we review our findings on the timing of reproduction and the potential reproductive rate of this *** investigate the first question,the reproductive condition of adult females,and occurrence of immatures,in a population on a cattle station in central Australia was determined and,for the second,data from a laboratory colony compared to those previously published on closely related species in other *** findings show that,at least in the population at the times that monitoring was performed,reproductive activity was only taking place in spring and early summer,whereas the laboratory study indicates that females have a similar gestation length and litter size to those of most close relatives occurring in other *** have extremely small testes and store relatively few *** findings suggest that Notomys alexis might show some seasonality of reproduction at least in this region of central Australia and that this species does not have a higher reproductive rate than that of related species in other,more predictable,environments.

关键词： arid environment hopping mouse reproduction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Analysis of spontaneous adverse drug reactions to echinacea, valerian,black cohosh and ginkgo in Australia from 2000 to 2015

引用

Journal of Integrative Medicine 2019年第5期17卷 338-343页

作者： Claire L.Hoban Roger W.Byard Ian F.Musgrave discipline of Pharmacology The University of AdelaideAdelaideSA 5005Australia discipline of anatomy and Pathology The University of AdelaideAdelaideSA 5005Australia

Objective: Assessing adverse drug reactions(ADRs) is a proven method to estimate the safety of medicines. The ADRs to herbal medicines in Australia(and by inference, the safety of herbal medicines in Australia) remain unknown. This study examines spontaneous ADR cases to four of the most popular herbs in Australia from 2000 to 2015: echinacea(Echinacea purpurea), valerian(Valeriana officinalis), black cohosh(Actaea racemosa) and ginkgo(Ginkgo biloba).Methods: ADRs of echinacea, valerian, black cohosh and ginkgo reported to the Australian Therapeutic Goods Administration(TGA) between 2000 and 2015 were obtained from the TGA database. Data were collated and analysed according to age, sex, severity, type of ADR and body system affected. Statistics were calculated using Graph Pad Prism ***: Most ADRs were mild or moderate. However, every herbal medicine was associated with lifethreatening ADRs. In each life-threatening case, the herbal medicine was taken concomitantly with prescription medications. Black cohosh was associated with a significant number of severe ADRs(30.3% of the total), with 39.4% of these ADRs being associated with abnormal hepatic function, hepatitis or ***: This study highlights the lack of public awareness with regard to herb–drug interactions,since most of the severe ADRs involved a herb–drug interaction.

关键词： Herbal medicine Adverse drug reaction Actaea racemosa Echinacea purpurea Ginkgo biloba Valeriana officinalis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Matrix stiffening promotes chondrocyte senescence and the osteoarthritis development through downregulating HDAC3

引用

Bone Research 2024年第2期12卷 438-452页

作者： Bowen Fu Jianlin Shen Xuenong Zou Nian Sun Ze Zhang Zengping Liu Canjun Zeng Huan Liu Wenhua Huang Guangdong Medical Innovation Platform for Translation of 3D Printing Application The Third Affiliated HospitalSouthern Medical UniversityGuangzhou 510630 GuangdongChina Guangdong Engineering Research Center for Translation of Medical 3D Printing Application Guangdong Provincial Key Laboratory of Digital Medicine and BiomechanicsNational Key Discipline of Human AnatomySchool of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510145 GuangdongChina Department of Foot and Ankle Surgery Center for Orthopedic SurgeryThe Third Affiliated HospitalSouthern Medical UniversityGuangzhou 510630 GuangdongChina Department of Orthopedics Affiliated Hospital of Putian UniversityPutian 351100 FujianChina Central Laboratory Affiliated Hospital of Putian UniversityPutian 351100 FujianChina Guangdong Provincial Key Laboratory of Orthopedics and Traumatology Department of Spinal SurgeryThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080 GuangdongChina Nanfang Hospital Southern Medical UniversityGuangzhou 510515 GuangdongChina Guangzhou Blood Center Guangzhou 510095 GuangdongChina Department of Orthopedics The Affiliated Traditional Chinese Medicine HospitalSouthwest Medical UniversityLuzhou 646000 SichuanChina

Extracellular matrix(ECM)stiffening is a typical characteristic of cartilage aging,which is a quintessential feature of knee osteoarthritis(KOA).However,little is known about how ECM stiffening affects chondrocytes and other molecules *** study mimicked the physiological and pathological stiffness of human cartilage using polydimethylsiloxane(PDMS)*** demonstrated that epigenetic Parkin regulation by histone deacetylase 3(HDAC3)represents a new mechanosensitive mechanism by which the stiffness matrix affected chondrocyte *** found that ECM stiffening accelerated cultured chondrocyte senescence in vitro,while the stiffness ECM downregulated HDAC3,prompting Parkin acetylation to activate excessive mitophagy and accelerating chondrocyte senescence and osteoarthritis(OA)in ***,intra-articular injection with an HDAC3-expressing adeno-associated virus restored the young phenotype of the aged chondrocytes stimulated by ECM stiffening and alleviated OA in *** findings indicated that changes in the mechanical ECM properties initiated pathogenic mechanotransduction signals,promoted the Parkin acetylation and hyperactivated mitophagy,and damaged chondrocyte *** results may provide new insights into chondrocyte regulation by the mechanical properties of ECM,suggesting that the modification of the physical ECM properties may be a potential OA treatment strategy.

关键词： chondrocyte osteoarthritis stiffness

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Zika virus infection and its emerging trends in Southeast Asia

引用

Asian Pacific Journal of Tropical Medicine 2017年第3期10卷 201-208页

作者： Ahmad Ruzain Salehuddin Haszianaliza Haslan Norshalizah Mamikutty Nurul Hannim Zaidun Mohamad Fairuz Azmi Mohamad Mu’izuddin Senin Syed Baharom Syed Ahmad Fuad Zar Chi Thent discipline of anatomy Faculty of MedicineUniversiti Teknologi MARASungai BulohSelangorMalaysia Klinik Dr.Muiz SelangorMalaysia

Zika virus is a mosquito-borne flavivirus that represents a public health emergency at the ongoing ***,this rare virus was limited to sporadic cases in Africa and Asia until its emergence in Brazil,South America in 2015,where it rapidly spread throughout the ***,a high number of cases were reported in Singapore and other Southeast Asia countries.A combination of factors explains the current Zika virus outbreak although it is highly likely that the changes in the climate and high frequency of travelling contribute to the spread of Aedes vector carrying the Zika virus mainly to the tropical climate countries such as the Southeast *** Zika virus is known to cause mild clinical symptoms similar to those of dengue and chikungunya and transmitted by different species of Aedes ***,neurological complications such as Guillain-Barrésyndrome in adults,and congenital anomalies,including microcephaly in babies born to infected mothers,raised a serious ***,there is no specific antiviral treatment or vaccine available for Zika virus ***,international public health response is primarily focused on preventing infection,particularly in pregnant women,and on providing up-to-date recommendations to reduce the risk of non-vector transmission of Zika virus.

关键词： Zika virus Emerging infection Microcephaly Neurological complication

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Targeting the body to protect the brain:inducing neuroprotection with remotely-applied near infrared light

引用

Neural Regeneration Research 2015年第3期10卷 349-351页

作者： Daniel M.Johnstone John Mitrofanis Jonathan Stone Bosch Institute University of Sydney discipline of Physiology University of Sydney discipline of anatomy & Histology University of Sydney

The incidence of intractable age-related neurodegenerative conditions such as Alzheimer＇s and Parkinson＇s diseases and age-related macular degeneration is projected to increase substantially over the coming decades with the ageing of the global population. While the burden of disease associ- ated with other chronic conditions has decreased in recent times due to improved diagnosis and treatment, current therapies for neurodegenerative diseases still fall short in that they are only effective in treating signs and symptoms - they do little to slow or prevent disease progress. Thus, there is an urgent need for treatments that address disease progression.

关键词： body MPTP Targeting the body to protect the brain MSCs

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Variations in wood anatomical properties and specific gravity of half sib progenies of Populus deltoides

引用

Journal of Forestry Research 2012年第3期23卷 491-496页

作者： P.K.Pande R.C.Dhiman Wood anatomy discipline Botany Division Institution:Forest Research InstituteDehradun India248006 WIMCO Seedling Ltd BagwalaRudrapur(Udhamsingh Nagar)India

We studied radial and inter-progeny variations in the dimen- sions of the wood elements and specific gravity of 21 half sib progenies of Populus deltoides Bartr. ex Marsh. The female parents of half sib progenies were G48 and $7C13 clones. Variance ratio （F） test indicated that inter-progeny variations in the dimensions of wood elements and specific gravity were significant for all wood traits while variations were significant for radial location for specific gravity only. Hierarchical clus- ter analysis was done by Squared Euclidean Distance for all of 21 proge- nies considering six wood traits. 21 progenies were grouped into 4 clus- ters. Cluster 1 was the largest cluster with 11 progenies, whereas cluster 3 had only one progeny. Selected progenies in clusters 3 （progeny 155, male） and 4 （progeny 108, 196, both female） were highly divergent from the other progenies so they were used in combinations as parents of hybrids to develop new clones with desired characters. Progeny 155 showed higher growth, fiber dimensions, and specific gravity, thus, should be used for the development of new clones.

关键词： Fiber length specific gravity vessel element length wall thickness wood variations

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Performance and variability patterns in wood properties and growth traits in the parents, F1 and F2 generation hybrid clones of Populus deltoides

引用

Journal of Forestry Research 2011年第3期22卷 379-385页

作者： P. K. Pande R. C. Dhiman Wood anatomy discipline Botany Division Forest Research Institute Dehradun (India)-248006 WIMCO Seedlings Ltd. Rudrapur Udhamsingh Nagar Uttarakhand India

The performance and variability patterns in the wood element’s dimensions, specific gravity and growth parameters namely ramet height and GBH were evaluated in 16 clones of parents, F1 and F2 hybrids of Populus deltoides Bartr. ex Marsh. Ramet radial variations were nonsignificant, while inter-clonal variations due to interaction of clone/replication were significant for all the wood traits except vessel element length. Inter-clonal variations were significant only for fiber length and fiber wall thickness. Fiber length and specific gravity were significantly higher in female, while wall thickness and vessel element length were higher in male clones. Female parents （G48 and S7C8） showed higher fiber length and specific gravity than of the male parent （G3）, while vessel diameter and wall thickness were higher in male par- ent （G3）. There is not much difference in fiber length and vessel element’s dimensions among the parents, F1 and F2 generation hybrid clones. Specific gravity did not showed any trend for parents, F1 and F2 generations. Generally female clones showed higher growth rate. Broad sense heritability for wood traits ranged from 0.143 （fiber length） to 0.505 （fiber wall thickness）, while for growth traits it was 0.374 （GBH） and 0.418 （height）. Genetic gain for all the wood and growth traits was positive for most of the wood traits. The highly divergent male clone （78） and female clones （S7C8, G48, W/A 49） in number of combinations could be used for developing new hybrids of desired wood traits to develop new clones.

关键词： Fiber length specific gravity vessel element length and wall thickness wood variations

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：