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用萤光抗体试验筛选能表达鸡马立克病病毒pp38基因的重组昆虫杆状病毒

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江苏农学院学报 1992年第1期13卷 1-5页

作者：崔治中 Lee L F dept.of anim.sci.and vet.med. Jiangsu Agric.Coll.Yangzhou 225001 美国农业部禽病及肿瘤实验室

用抗鸡马立克病病毒(MDV)的38Kd磷蛋白的单克隆抗体作萤光抗体试验,直接从用可转染性野生型杆状病毒AcMNPV株DNA与含MDVpp38基因的重组转基因质粒载体DNA共同转染的Sf 9单层细胞培养中筛选到能表达pp38基因产物的重组杆状病毒感染斑,并... 详细信息

用抗鸡马立克病病毒(MDV)的38Kd磷蛋白的单克隆抗体作萤光抗体试验,直接从用可转染性野生型杆状病毒AcMNPV株DNA与含MDVpp38基因的重组转基因质粒载体DNA共同转染的Sf 9单层细胞培养中筛选到能表达pp38基因产物的重组杆状病毒感染斑,并从保存于琼脂醣凝胶上的复印斑中收复并进一步克隆了该基因重组病毒.该法易于实施,即使未能熟练掌握根据杆状病毒多角体来区别野生型杆状病毒斑与基因重组病毒斑的技术,也可顺利地筛选到能表达目的基因的重组合病毒。

关键词：萤光抗体马立克氏病病毒鸡

Expression of interleukin 6 in brain and colon of rats with TNBS-induced colitis

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World Journal of Gastroenterology 2010年第18期16卷 2252-2259页

作者： Wang, Kai Yuan, Chuan-Ping Wang, Wei Yang, Zhan-Qing Cui, Wei Mu, Lian-Zhi Yue, Zhan-Peng Yin, Xiu-Ling Hu, Zhong-Ming Liu, Ju-Xiong Jilin Univ Coll Anim Sci & Vet Med Jilin Prov Key Lab Anim Embryo Engn Changchun 130062 Jilin Province Peoples R China Univ Hebei No Dept Vet Med Zhangjiakou 075029 Hebei Province Peoples R China Acad Mil med sci Beijing 100071 Peoples R China

AIM:To characterise expression of interleukin 6(IL-6),a potent proinflammatory cytokine,in the occurrence and development of inflammatory bowel disease(IBD) and investigate its effect on neuroimmunomodulation and immune homeostasis ***:In this study,rats with colitis induced by trinitrobenzene sulfonic acid(TNBS) were sacrificed on days 3,7,14,21 and 28 after *** the controls,the TNBS was just replaced by equivalent amount of phosphate buffered solution(PBS,0.01 mol/L).IL-6 mRNA expression in brain and colon tissues in each phase was evaluated by real-time reverse transcriptionpolymerase chain reaction,and cellular localisation and protein level of IL-6 was determined by ***:At day 7,mRNA expression of IL-6 was significantly higher in the colon and brain of IBD rats than that of the *** protein level was also significantly higher in colon,hypothalamus and cerebral cortex of IBD rats compared with the *** there are similar temporal trends in IL-6 mRNA expression and protein levels in all positions with a persistent increase to a peak at day 7,followed by a decline and gradual return to normal ***:These results revealed that changes in IL-6 expression in brain and colon tissues occur in different phases of ***,we propose that the nerve centre regulates and controls the occurrence and development of IBD via IL-6.

关键词： Inflammatory bowel disease Interleukin 6 Neuroimmunomodulation Real-time transcription-polymerase chain reaction Immunohistochemistry

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动物行为学——畜牧学的新分支学科

草食家畜 1984年第S1期 30-40页

作者： S.E.Curtis K.A.Houpt 刘敏雄 Cornell Univ. dept.of anim.Sct. dept.of Physiol. NY State College of vet.med. Uniy.of Illinois.

在过去的25年中,研究动物行为的科学—动物行为学(Ethology),业已发展成为畜牧学中一门成熟的分支学科。这一领域中的研究活动和教学素材已显示出不断增长的迹象。但是,总体说来还是不完整的,在动物行为学原理的认识方面以及畜牧科学工... 详细信息

在过去的25年中,研究动物行为的科学—动物行为学(Ethology),业已发展成为畜牧学中一门成熟的分支学科。这一领域中的研究活动和教学素材已显示出不断增长的迹象。但是,总体说来还是不完整的,在动物行为学原理的认识方面以及畜牧科学工作者和生产者应用这些原理方面,还留下很多空白。本文重点放在近代和当前在动物行为学研究中被选择的若干领域,试图在研究的深度和在动物管理中最终应用这些资料的潜在可能两方面做出说明。有关今后关注的问题也做讨论。

关键词：动物行为学分支学科分支科学动物安宁动物福利农业生产畜牧生产牧场家畜农畜仔猪母畜仔畜母猪公畜畜牧学

Studying the effect of formic acid and potassium diformate on performance,immunity and gut health of broiler chickens

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animal Nutrition 2016年第4期2卷 296-302页

作者： Naela M.Ragaa Reda M.S.Korany Nutrition and Clinical Nutrition dept. Fac.Vet.Med. Cairo University Pathology dept. Fac.Vet.Med. Cairo University

Our trial was conducted to study the effects of formic acid(FA) and potassium di-formate(KDF) in broiler ration on performance, carcass traits, blood biochemical, intestinal microbial load, histological picture of intestine and immune parameters of broilers. In this study 360 one-day-old broiler chicks were divided to 3 groups with 3 replicates of 40 chicks each. The trial continued for 35 days. The control group was fed only basal diet(G1). Group 2(G2) were fed basal diet supplemented with FA(5 g/kg diet), and group 3(G3) received basal diet supplemented with KDF(5 g/kg diet). The results showed that both FA and KDF significantly increased body weight gain(BWG), dressing percentage of broilers and significantly decreased feed conversion ratio(FCR)(P < 0.05). The highest percent of breast and thigh was observed in G3. The improvement in villus height was observed in G2 and G3 compared with the control one, and the highest was in G3. The results evidence that the using of FA or KDF in broiler feeds have significant effects on performance, immune parameters, and gut health without having any significant effects on blood biochemical. However, KDF is more effective than FA as little amount of FA reaches the small intestine due to metabolism and absorption, whereas KDF permits a proportion of FA to pass through the fore-gut intact and enter the small intestinal tract. In addition, FA has a strong odor and corrosiveness to gastrointestinal tract which limits its use.

关键词： Acidifiers Broiler Growth parameters Gut health

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发情周期生理

草食家畜 1984年第S1期 99-113页

作者： William Hansel,Edward M.Convey,董伟 dept.of Physiol. NY State College of vet.med. Cornell Univ. Aerck Sharp and Dohme Research.Laboratories Rahway NJ.

文中对牛、羊、猪的卵泡生长和排卵,丘脑下部—垂体—卵巢的互作,黄体功能和发情周期的调节等问题的现有观点和新的提法进行了综述。在整个发情周期中,卵泡持续不断地生长、退化和被其他的大卵泡代替。在这个过程中,卵巢内部因素似乎起... 详细信息

文中对牛、羊、猪的卵泡生长和排卵,丘脑下部—垂体—卵巢的互作,黄体功能和发情周期的调节等问题的现有观点和新的提法进行了综述。在整个发情周期中,卵泡持续不断地生长、退化和被其他的大卵泡代替。在这个过程中,卵巢内部因素似乎起重要作用。排卵前卵泡内雌二醇的浓度在LH的排卵前高峰到来之前一直增长,然后急速下降。人们提出了一个所谓控制卵泡类固醇合成的二细胞—二促性腺激素的模式,这个概念还提出,卵泡的雄激素是通过A5通路合成的,细胞内高浓度的雌二醇在LH峰出现之前抑制着孕酮的生产。有人认为卵巢组织能生产非类固醇的FSH和丘脑下部激素的抑制剂。我们的现代概念大概只是对黄体退化时发生的情况的部分解释。有例证说明前列环素（prostacyclin）和PGE2在黄体生长期分别对母牛和绵羊具有促黄体的作用。最近的一些研究认为催产素对牛、羊的黄体退化可能具有生理功能。磷酯的甲基化,看来对LH刺激黄体细胞腺甙酸环化酶（adeny-late cyclase）和后来孕酮生产的机制,是一个重要的调节步骤。促性腺激素的分泌有明显的间歇,依周期阶段和生殖状态而变化。引用的文献与下述观点是相吻合的,即在GnRH的脉冲式释放的连续影响和雌激素的控制下,LH的脉冲式释放量也依次逐渐增多,从而产生了排卵前的LH峰。已

关键词：卵泡闭锁 FSH LH 卵巢疾病孕酮孕激素雌二醇雌激素发情周期促性腺激素激素类药物卵巢性腺排卵前黄体细胞

Action mechanisms of a new erythrocyte-derived depressing factor

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Chinese science Bulletin 2000年第23期45卷 2113-2118页

作者： Yunyi Wen Xiaochun Zhang Xiaorong Xu Guiqing Zhao Fang Wan Peking Union med Coll Inst Basic Med Sci Dept Physiol Sch Basic MedChinese Acad Med Sci Beijing 100005 Peoples R China

To investigate the action mechanisms of a new erythrocyte-derived depressing factor(EDDF), the focus is placed on the effect of EDDF on both cytosolic and nuclear free calcium (Ca2+) transportation in vascular smooth muscle cell (VSMC), as well as the apoptosis and cell cycle of VSMC of rats. EDDF has been extracted from human erythrocytes. The changes of Ca2+ levels in cytoplasm ([Ca2+],) and nucleus ([Ca2+]n) have been observed using a laser scanning confocal microscope together with fluo-3/AM as a calcium indicator. Flow cytometric technique was used to study the effect of EDDF on cell cycle and apoptosis of VSMC. [Ca2+]j and [Ca2+]n were significantly decreased through several different pathways: ( i) it reduced the Ca2+ influx by blocking L-type voltage-dependent calcium channel (L-VDC) and R-type voltage-dependent calcium channel (R-VDC); (ii) it inhibited the Ca2+ release from inositol 1, 4, 5-trisphosphate (IP3) sensitive calcium store; and (iii) activated Ca2+-ATPase of sarcoplasmic reticulum (

关键词： erythrocyte-derived depressing factor (EDDF) calcium transportation cell cycle apoptosis vascular smooth muscle ceil.

Study on 3D Quantitative Structure-Activity Relationships of Taxoid

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Chinese Chemical Letters 1997年第10期8卷 893-896页

作者： Zhu, QQ Guo, ZR Huang, N Wang, MM Chu, FM CHINESE ACAD med sci INST MAT MEDDEPT SYNTH MED CHEMBEIJING 100050PEOPLES R CHINA

A series of 94 taxol analogues exhibiting antitumor activity were investigated using comparative molecular field analysis (CoMFA) method. The most optimal CoMFA from 80 compounds selected randomly yielded a two-components model, with significant cross-validation r(cv)(2) of 0.640 and conventional r(2) of 0.868. The robustness of the CoMFA model was further validated by prediction of 14 test set compounds. The CoMFA model explained present SAR and particularly the importance of the hydroxyl group at C-2' position and the stereochemistry at C-2' and C-3' positions.

关键词： Activity Study on 3D Quantitative Structure-Activity Relationships of Taxoid

Adhesion and immunomodulatory effects of Bifidobacterium lactis HN019 on intestinal epithelial cells INT-407

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World Journal of Gastroenterology 2010年第18期16卷 2283-2290页

作者： Liu, Chang Zhang, Zhuo-Yang Dong, Ke Guo, Xiao-Kui Shanghai Jiao Tong Univ Sch Med Inst Med Sci Dept Med Microbiol & Parasitol Shanghai 200025 Peoples R China

AIM:To elucidate the adherence and immunomodulatory properties of a probiotic strain Bifidobacterium lactis(***) ***:Adhesion assays of *** HN019 and Salmonella typhimurium(***) ATCC 14028 to INT-407 cells were carried out by detecting copies of species-specific genes with real-time polymerase chain *** study was further conducted by transmission electron ***-1β(IL-1β),interleukin-8,and tumor necrosis factor-α(TNF-α) gene expression were assessed while enzyme linked immunosorbent assay was used to detect IL-8 protein ***:The attachment of *** ATCC 14028 to INT407 intestinal epithelial cells was inhibited significantly by *** *** HN019 could be internalized into the INT-407 cells and attenuated IL-8 mRNA level at both baseline and *** pro-inflammatory ***-8 secretion was reduced while IL-1β and TNF-α mRNA expression level remained unchanged at baseline after treated with *** ***:*** HN019 does not up-regulate the intestinal epithelium expressed pro-inflammatory cytokine,it showed the potential to protect enterocytes from an acute inflammatory response induced by enteropathogen.

关键词： Probiotics Intestinal epithelium Enteropathogen Immunomodulation Adhesion

Protective role of a novel human erythrocyte-derived depressing factor on blood vessels in rats

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Chinese science Bulletin 2002年第9期47卷 717-721页

作者： Huan Pang Yunyi Wen Lei Shi Ning Ma Yutang Wang Chinese Acad med sci Inst Basic Med Sci Dept Physiol Sch Basic MedPeking Union Med Coll Beijing 100005 Peoples R China

The protective role of a human erythro-cyte-derived depressing factor (EDDF) on blood vessels was evaluated. The experiments were carried out on 25 male Wistar rats aged 6-8 weeks, which were divided into control (n=8), calcium overload (n=8) and NG-L-nitro-arginine hypertensive model groups (L-NNA, n = 9), respectively. The isolated vascular ring perfusion assay, two-photon laser scanning fluorescence microscopy (TPM) and transmitted electron microscope were used to examine the effect of EDDF on vascular function and ultrastructure. Results showed that the contractile response of calcium overload rats and L-NNA rats to phenylephrine (PE) was significantly enhanced compared with that of the control (P < 0.05), and EDDF (10-3g·mL-1) remarkably decreased the vascular contractile response of control’s and calcium overload rats (P < 0.05), while EDDF had no effect on that of L-NNA rats. EDDF also alleviated the ultrastructural lesion of aorta VSMC in calcium overload rats by easing the abnormal in the

关键词： erythrocyte-derived depressing factor calcium over-load NG-L-nitro-arginine vascular ring two-photon laser scan-ning fluorescence microscope ultrostructure.