Action mechanisms of a new erythrocyte-derived depressing factor

作     者：Yunyi Wen Xiaochun Zhang Xiaorong Xu Guiqing Zhao Fang Wan 

作者机构：Peking Union Med Coll Inst Basic Med Sci Dept Physiol Sch Basic MedChinese Acad Med Sci Beijing 100005 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2000年第45卷第23期

页      面：2113-2118页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：97 1002 39370268 

主　　题：erythrocyte-derived depressing factor (EDDF) calcium transportation cell cycle apoptosis vascular smooth muscle ceil. 

摘      要：To investigate the action mechanisms of a new erythrocyte-derived depressing factor(EDDF), the focus is placed on the effect of EDDF on both cytosolic and nuclear free calcium (Ca2+) transportation in vascular smooth muscle cell (VSMC), as well as the apoptosis and cell cycle of VSMC of rats. EDDF has been extracted from human erythrocytes. The changes of Ca2+ levels in cytoplasm ([Ca2+],) and nucleus ([Ca2+]n) have been observed using a laser scanning confocal microscope together with fluo-3/AM as a calcium indicator. Flow cytometric technique was used to study the effect of EDDF on cell cycle and apoptosis of VSMC. [Ca2+]j and [Ca2+]n were significantly decreased through several different pathways: ( i) it reduced the Ca2+ influx by blocking L-type voltage-dependent calcium channel (L-VDC) and R-type voltage-dependent calcium channel (R-VDC); (ii) it inhibited the Ca2+ release from inositol 1, 4, 5-trisphosphate (IP3) sensitive calcium store; and (iii) activated Ca2+-ATPase of sarcoplasmic reticulum (