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北京大学学报(医学版) 1989年第5期 437-445页

作者：陈慰峰李萌李晨张惠华樊景禹方伟岗 dept. of Immunology dept. of Biophysics Depl. of pathology Beijing Medical University

A murine C2 early T cell line derived from spleen cell cultures of Balb/C mice has been established. C2 cells express the phenotype of Thy-1+CD4-CD8-Slg-, and their growth is exogenous factor-independent. Under electron-microscope examination, virus particles are clearly visualized present in the vacuoles of the cytoplasm near cell membrane and released outside the cells. A high activity of reverse transcriptase encoded by retrovirus RNA has been measured in the virus-enriched compartment isolated from C2 cells. C2 cells are tumourigenic as shown by their ability to cause diffused tumours in nude mice. After stimulation with phorbol ester and calcium ionophore, C2 cells produced a variety of lymphokines, including 1L-2, IL-4, IFNr and MAP with high or moderate activity.

关键词： Tumourigenic early T Cell line Retrovirus Reverse transcriptase Factor-independent growth lymphokines

Cloning of Mouse Enamel Matrix Serine Proteinase Encoding Mature Protein

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吉林大学学报（医学版） 2003年第6期29卷 705-709页

作者： MU Ya bing, SUN Hong chen, ZHANG Ze bing, OUYANG Jie (dept. of pathology, School of Stomatology, Jilin University,Changchun 130041,China) dept. of pathology School of Stomatology Jilin University Changchun 130041

目的 :克隆小鼠牙胚组织中釉基质丝氨酸蛋白酶 ( EMSP1 )成熟肽编码区基因。方法 :提取出生后 7d昆明种小白鼠切牙、磨牙牙胚总 RNA,逆转录为 c DNA,设计两对特异性引物 ,采用 Touchdown PCR和嵌套 PCR方法 ,扩增出小鼠 EMSP1起始密码... 详细信息

目的 :克隆小鼠牙胚组织中釉基质丝氨酸蛋白酶 ( EMSP1 )成熟肽编码区基因。方法 :提取出生后 7d昆明种小白鼠切牙、磨牙牙胚总 RNA,逆转录为 c DNA,设计两对特异性引物 ,采用 Touchdown PCR和嵌套 PCR方法 ,扩增出小鼠 EMSP1起始密码子至终止密码子基因片段。将目的基因连入载体 p MD- 1 8T,转化入大肠杆菌 JM1 0 9,通过蓝白筛选 ,挑选阳性克隆培养扩增 ,纯化重组质粒进行限制性酶切和核苷酸序列分析鉴定。结果 :限制性酶切图谱和核苷酸序列分析均表明所克隆 c DNA为小鼠 70 0 bp的 EMSP1成熟肽基因编码。结论

关键词：牙胚小鼠丝氨酸内肽酰类逆转录聚合酶链反应聚合酶链反应克隆分子

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Studying the effect of formic acid and potassium diformate on performance,immunity and gut health of broiler chickens

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Animal Nutrition 2016年第4期2卷 296-302页

作者： Naela M.Ragaa Reda M.S.Korany Nutrition and Clinical Nutrition dept. Fac.Vet.Med. Cairo University pathology dept. Fac.Vet.Med. Cairo University

Our trial was conducted to study the effects of formic acid(FA) and potassium di-formate(KDF) in broiler ration on performance, carcass traits, blood biochemical, intestinal microbial load, histological picture of intestine and immune parameters of broilers. In this study 360 one-day-old broiler chicks were divided to 3 groups with 3 replicates of 40 chicks each. The trial continued for 35 days. The control group was fed only basal diet(G1). Group 2(G2) were fed basal diet supplemented with FA(5 g/kg diet), and group 3(G3) received basal diet supplemented with KDF(5 g/kg diet). The results showed that both FA and KDF significantly increased body weight gain(BWG), dressing percentage of broilers and significantly decreased feed conversion ratio(FCR)(P < 0.05). The highest percent of breast and thigh was observed in G3. The improvement in villus height was observed in G2 and G3 compared with the control one, and the highest was in G3. The results evidence that the using of FA or KDF in broiler feeds have significant effects on performance, immune parameters, and gut health without having any significant effects on blood biochemical. However, KDF is more effective than FA as little amount of FA reaches the small intestine due to metabolism and absorption, whereas KDF permits a proportion of FA to pass through the fore-gut intact and enter the small intestinal tract. In addition, FA has a strong odor and corrosiveness to gastrointestinal tract which limits its use.

关键词： Acidifiers Broiler Growth parameters Gut health

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Nodular lymphoid hyperplasia: A marker of low-grade inflammation in irritable bowel syndrome?

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World Journal of Gastroenterology 2016年第46期22卷 10198-10209页

作者： Anna Chiara Piscaglia Lucrezia Laterza Valentina Cesario Viviana Gerardi Rosario Landi Loris Riccardo Lopetuso Giovanni Calò Giovanna Fabbretti Massimo Brisigotti Maria Loredana Stefanelli Antonio Gasbarrini Endoscopy and Gastroenterology Unit Depatment of Internal Medicine State Hospital School of Gastroenterology Dept. of Internal Medicine and Gastroenterology "Gemelli" Hospital Catholic University Depatment of pathology "Infermi" Hospital

AIM To evaluate the prevalence of nodular lymphoid hyperplasia(NLH) in adult patients undergoing colonoscopy and its association with known diseases. METHODS We selected all cases showing NLH at colonoscopy in a three-year timeframe, and stratified them into symptomatic patients with irritable bowel syndrome(IBS)-type symptoms or suspected inflammatory bowel disease(IBD), and asymptomatic individuals undergoing endoscopy for colorectal cancer *** collection included medical history and final diagnosis. As controls, we considered all colonoscopies performed for the aforementioned indications during the same *** One thousand and one hundred fifty colonoscopies were selected. NLH was rare in asymptomatic individuals(only 3%), while it was significantly more prevalent in symptomatic cases(32%). Among organic conditions associated with NLH, the most frequent was IBD, followed by infections and diverticular disease. Interestingly, 31% of IBS patients presented diffuse colonic NLH. NLH cases shared some distinctive clinical features among IBS patients: they were younger, more often female, and had a higher frequency of abdominal pain, bloating, diarrhoea, unspecific inflammation, self-reported lactose intolerance and metal contact dermatitis. CONCLUSION About 1/3 of patients with IBS-type symptoms or suspected IBD presented diffuse colonic NLH, which could be a marker of low-grade inflammation in a conspicuous subset of IBS patients.

关键词： Inflammatory bowel diseases Functional gastrointestinal diseases Irritable bowel syndrome Colonoscopy Inflammation

Monoclonal antibodies that target the immunogenic proteins expressed in colorectal cancer

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World Journal of Gastrointestinal Oncology 2014年第6期6卷 170-176页

作者： Myron Arlen Philip Arlen Gene Coppa Jim Crawford XuePing Wang Olga Saric Alex Dubeykovskiy Ernesto Molmenti Department of Surgery Division of Surgical Oncology and the Dept. of Pathology North Shore University HospitalManhasset NY 11030 United States Manhasset NY and Precision Biologics Great Neck NY 11021 United States

In an attempt to improve upon the end results obtained in treating colorectal cancer it was apparent that the earlier the diagnosis that could be obtained, the better the chance for obtaining desired results. In the case of more advanced tumors typified by later stage colorectal cancer, surgical debulking is an important part of the treatment strategy. Here the use of additional therapeu-tic modalities including chemotherapy and present day immunotherapy has failed to accomplish the desired im-provements that have been sought after. Adjuvant ther-apy, has offered little to the overall survival. The concept of early detection is now recognized as the initial step in reaching proper end results and can readily be demon-strated from colorectal cancer studies. Here survival has been found to be a reflection of the stage at which the tumor is first identified and treated. When specific mono-clonals targeting colorectal cancer are employed diagnos-tically, we have been able to demonstrate detection of colorectal cancer at its inception as a premalignant lesion, such that genotypic features can be identified before the phenotypic appearance of cancer can be noted.

关键词： Colorectal cancer Monoclonal antibodies Immunohistochemistry Antibody dependent cell cytotoxicity Tumor associated antigens

Comparative Pertussis Antibody Response of Nigerian Children to Diphtheria, Pertussis, Tetanus (DPT) and Pentavalent Vaccines

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World Journal of Vaccines 2020年第3期10卷 43-53页

作者： Emmanuel Eyo Ekanem Glory Ekpo Bassey Henry Chima Okpara Eyong Komomo Ibor dept. of Paediatrics University of Calabar and University of Calabar Teaching Hospital Calabar Nigeria dept. of Chemical pathology University of Calabar and University of Calabar Teaching Hospital Calabar Nigeria

Background: In Nigeria Pentavalent vaccine had replaced Diphtheria-Pertussis- Tetanus [DPT] vaccine in the prevention of pertussis since 2012. Aims and Objectives: The aim of this study was to compare the anti-pertussis immunoglobin G (IgG) response of children who received DPT with those who received the pentavalent vaccine. Subjects and Methods: This study was carried out in Akpabuyo LGA of Cross River State from April to June 2016. It was a cross-sectional survey of anti-pertussis IgG levels in children aged 6 months to 5 years who received DPT and those who received pentavalent vaccine. IgG antibody levels were determined using enzyme-linked immunosorbent assay. The protective level was set at >11 DU according to manufacturer’s cut off point. Results: Seventy eight out of 230 children [33.9%] who had received DPT had protective levels of anti-pertussis IgG compared to 74 out of 192 children [38.5%] who had received pentavalent vaccine. The difference was not statistically significant [p = 0.61]. The median IgG antibody level in those who received DPT was 8.0 DU (interquartile range (IQR) 4.0 - 13.0) compared with 9.0 DU (IQR) 4.0 - 15.0 in those who received pentavalent vaccine [p = 0.18]. No single factor investigated predicted the development of protective levels of antibody in the multivariate analysis. Conclusion/Recommendation: There was no difference in the antipertussis antibody response between DPT and pentavalent vaccines recipients. Further study is needed to elucidate factors that could be responsible for low anti-pertussis antibody response in this population.

关键词： Anti-Pertussis Immunoglobulin G Diphtheria-Pertussis-Tetanus Pentavalent Vaccine Comparative

Speciation studies of arsenic in the environment and in human

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Chinese Journal Of Geochemistry 2006年第B8期25卷 77-78页

作者： X. Chris Le Nicole Orr Department of Public Health Sciences and dept. of Laboratory Medicine and pathology University of Alberta Edmonton Canada

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Cytokine network and tuberculosis

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Cytokine network and tuberculosis

Controlling of Fusarium Wilt of Cucumber by Antagonistic Bacteria

中华医学会第六届全国结核病学术大会

作者： Lsamu Sugawara MD PhD Head of Molecular Pathology Dept.The Research Institute of TuberculosisTokyoJapan

Tuberculosis is a form of chronic specific inflammation induced by M. tuberculosis. Many lines of evidence show that various cytokines are closely involved in M. tuberculosis- induced granulation formation. In order t...

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Journal of Life Sciences 2010年第7期4卷 16-21页

作者： Gamal Ashour Ahmed Plant pathology Branch Agric. Botany Dept. Fac. Agric. at Moshtohor Benha University Benha 13736 Egypt

Studying about the effect of four pseudomonas and two serratia isolates on growth of Fusarium oxysporum showed that, Pseudomonasfluorescens No.2 ＆ No.3 and Serratia marcescens No.2 gave highest inhibition zones which were 37.33, 35,00 and 31.33 mm, respectively. Evaluating about the effect ofpseudomonas and serratia culture filtrates at three concentrations （10, 25, 50%） on the linear growth and spore germination ofFusarium oxysporum revealed that, all filtrates of the tested isolates reduced the mycelial growth and spore germination of F. oxysporum. All filtrates of the tested isolates at 50% concentration completely inhabited spore germination ofF. oxysporurn. Culture filtrates of Pseudomonasputida and Serratia marcensens No.2 at 50% concentration reduced the mycelial growth of F. oxysporurn by 80.74 and 80.37% respectively. In this respect, all tested isolates made lysis to mycelial of F. oxysporum except Pseudornonasfluorescens No.3. Effect of treating cucumber seeds with cell suspension of pseudomonas and serratia isolates on incidence of Fusarium wilt disease revealed that all isolates were effective in reducing disease incidence and disease severity and increasing the percentage of healthy plants compared to the control. Pseudornonasfluorescens No.3 and Serratia marcensens No.2 were the best isolates and completely prevented the disease incidence.

关键词： Cucumber fusarium wilt pseudomonas serratia and greenhouses.

Pleural Effusion in a Dog with Mesothelioma

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Journal of Life Sciences 2015年第8期9卷 362-365页

作者： Sinem Ulgen Utku Bakirel Kutay Yildiz Funda Yildirim Ibrahim Firat Istanbul University Faculty of Veterinary Medicine Dept. of lnternal Medicine Avctlar lstanbu134320 Turkey Istanbul University Faculty of Veterinary Medicine Dept. of Pathology Avcdar Istanbu134320 Turkey

In this case; mesothelioma was likely diagnosed in 7 years old male mix breed dog which was brought to Veterinary Research and Teaching Hospital of Istanbul University with complaints of general weakness and severe dyspnea. The dog had been misdiagnosed with faryngitis and hernia diaphragmatica before it was brought to our faculty clinic. Apparent abdominal respiration was inspected and supressed heart sounds, abnormal respiratory sounds and pleural rub were detected by auscultation of thorax. Radiographic examination and blood analysis were performed. Chest X-ray examination demonstrates both sided pleural effusion. Leukocytosis （WBC = 36.2 × 10^3μL） was detected on blood count. By thoracocentesis, 40 mL fluid was drained and cytologic examination was performed. Numerous pleomorphic, atypical, multinuclear or multinucleolar mesothelial cells were observed and cytologic findings were pretty compatible with mesothelioma. The dog died before medication and the owner of the dog didn＇t give the confirmation for necropsy. In our clinical case we did not confirm any contact of the dog with asbestos or larger amount of pesticides, which are described as etiological factors of mesothelioma. As mesothelioma is a very rare health condition for dogs, it might be lead up to misdiagnosis.

关键词： Mesothelioma pleuraleffusion dog veterinarymedicine