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World Journal of Gastroenterology 2024年第18期30卷 2391-2396页

作者： Michele Barone Section of Gastroenterology Department of Precision and Regenerative Medicine-Jonian Area-University of BariBari 70124Italy

This editorial contains comments on the article by Zhao et al in print in the World Journal of *** mechanisms responsible for hepatic fibrosis are also involved in ***,we recapitulated the complexity of the renin-angiotensin system,discussed the role of hepatic stellate cell(HSC)autophagy in liver fibrogenesis,and analyzed the possible implications in the development of hepatocarcinoma(HCC).Angiotensin-converting enzyme inhibitors and angiotensin receptor blockers definitively contribute to reducing hepatic fibrogenesis,whereas their involvement in HCC is more evident in experimental conditions than in human ***-converting enzyme 2(ACE2),and its product Angiotensin(Ang)1-7,not only regulate HSC autophagy and liver fibrosis,but they also represent potential targets for unexplored applications in the field of ***,ACE2 overexpression inhibits HSC autophagy through the AMP-activated protein kinase(AMPK)/mammalian target of rapamycin(mTOR)*** this case,Ang 1-7 acts binding to the MasR,and its agonists could modulate this ***,since AMPK utilizes different targets to suppress the mTOR downstream complex mTOR complex 1 effectively,we still need to unravel the entire pathway to identify other potential targets for the therapy of fibrosis and liver cancer.

关键词： Renin-angiotensin system Liver fibrosis Hepatic stellate cells Autophagy Hepatocellular carcinoma

RhoGTPases in stem cells

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Chinese Science Bulletin 2007年第22期52卷 3025-3028页

作者： YAO HaiLei XIE XiaoYan WANG DongMei PEI XueTao department of Stem Cells and regenerative Medicine Beijing Institute of Transfusion MedicineBeijing 100850 China

RhoGTPases are small molecules that control a wide variety of signal transduction pathways. Their profound function in regulating the actin cytoskeleton is well recognized. Stem cells are unique in their ability to self-renew and produce progenitor cells that can differentiate into specialized cells. RhoGTPases influence stem cell morphology and cell migration as well as stem cell self-renewal,proliferation,transplantation,homing and differentiation. In this review,the multiple roles of the RhoGTPases in stem cells are discussed.

关键词：干细胞自我更新能力转换方式调节作用

Complete reprogramming to all-iPS mice

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Protein & Cell 2010年第1期1卷 2-3页

作者： Steven Y Cheng department of Developmental Genetics and Center for regenerative Medicine Nanjing Medical UniversityNanjing 210029China

The landmark discoveries by Shinya Yamanaka in mice and later separately by James Thompson in humans that adult animal cells can be reprogrammed to a pluripotent state of embryonic stem(ES)cells by forced expression of just a few key transcription factors have permanently altered a long-held view about the stability of differentiated cells in developmental biology,and suggested a way of generating personalized replacement tissues from patients’own cells for the regenerative medical treatment.

关键词： programming generating permanent

Effects of clenbuterol on the expression of adiponectin in 3T3-L1 adipocytes

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Journal of Reproduction and Contraception 2015年第4期26卷 197-203页

作者： Hai-ting YANG Wei-jie ZHU department of Developmental and regenerative biology College of Life Science and Technology Jinan University

Objective adiponectin Methods To investigate the effects of clenbuterol （CLB） on the expression of （ADP） in adipocytes. 3T3-L1 pre-adipocytes were induced into mature adipocytes, and then randomly divided into four groups based on doses of CLB： 0 12mol/L （group A, as the control）, 0.5 12mol/L （group B, low-dose group）, 5 μmol/L （group C, mid-dose group）, and 50 μmol/L （group D, high-dose group）, respectively. These four groups were cultured for both 12 h and 24 h. After CLB exposure, the effects of CLB on the expression of ADP in adipocytes were detected by qPCR and Western blotting analysis, and cell viability was quantified by the methyl thiazolyl tetrazolium assay. Results At 12 h, there was no difference in the expression of ADP between group A and group B or C （P〉0.05）. With the increase of CLB doses, the expression level of ADP in group D was lower than that in group A （P〈0.01）. After 24 h of incubation, compared with group A, there was a greater decrease in the expression of ADP in group B （P〈0.05）, and this suppression was more remarkable in group C or D （P〈O.O1）. At 12 h, viability of the cells in group B had no difference compared with group A （P〉 0.05）. A significant decrease of cell viability was counted in group C or D （P〈0.05）. At 24 h, with the increasing doses of CLB, viability of the cells showed more severe decrease in three experimental groups compared with the control （P〈0.01）. Conclusion After CLB exposure, the expression of ADP could be down-regulated and the decrease was more severe with the increasing doses of CLB.

关键词： clenbuterol (CLB) adipocyte adiponectin (ADP) lipid metabolism reproductive disease

Inducing human induced pluripotent stem cell differentiation through embryoid bodies:A practical and stable approach

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World Journal of Stem Cells 2020年第1期12卷 25-34页

作者： Ning-Ning Guo Li-Ping Liu Yun-Wen Zheng Yu-Mei Li Institute of regenerative Medicine Affiliated Hospital of Jiangsu UniversityJiangsu UniversityZhenjiang 212001Jiangsu ProvinceChina department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery University of Tsukuba Faculty of MedicineTsukubaIbaraki 305-8575Japan Yokohama City University School of Medicine YokohamaKanagawa 234-0006Japan Division of regenerative Medicine Center for Stem Cell Biology and Regenerative MedicineThe Institute of Medical Sciencethe University of TokyoTokyo 108-8639Japan

Human induced pluripotent stem cells(hiPSCs)are invaluable resources for producing high-quality differentiated cells in unlimited quantities for both basic research and clinical *** are particularly useful for studying human disease mechanisms in vitro by making it possible to circumvent the ethical issues of human embryonic stem cell ***,significant limitations exist when using conventional flat culturing methods especially concerning cell expansion,differentiation efficiency,stability maintenance and multicellular 3D structure establishment,differentiation *** bodies(EBs),the multicellular aggregates spontaneously generated from iPSCs in the suspension system,might help to address these *** to the unique microenvironment and cell communication in EB structure that a 2D culture system cannot achieve,EBs have been widely applied in hiPSC-derived differentiation and show significant advantages especially in scaling up culturing,differentiation efficiency enhancement,ex vivo simulation,and organoid *** can potentially also be used in early prediction of iPSC differentiation *** improve the stability and feasibility of EB-mediated differentiation and generate high quality EBs,critical factors including iPSC pluripotency maintenance,generation of uniform morphology using micro-pattern 3D culture systems,proper cellular density inoculation,and EB size control are discussed on the basis of both published data and our own laboratory ***,the production of a large quantity of homogeneous EBs with high quality is important for the stability and feasibility of many PSCs related studies.

关键词： Induced pluripotent stem cells Suspension culture Embryoid body Early prediction Committed differentiation Heterogeneity Three-dimensional culture Scaling-up Quality control

Fabrication of 3D-printed molds for polydimethylsiloxane-based microfluidic devices using a liquid crystal display-based vat photopolymerization process:printing quality,drug response and 3D invasion cell culture assays

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Microsystems & Nanoengineering 2023年第6期9卷 205-219页

作者： Matthew D.Poskus Tuo Wang Yuxuan Deng Sydney Borcherding Jake Atkinson Ioannis K.Zervantonakis department of Bioengineering UPMC Hillman Cancer CenterUniversity of PittsburghPittsburghPAUSA McGowan Institute of regenerative Medicine PittsburghPAUSA

Microfluidic platforms enable more precise control of biological stimuli and environment dimensionality than conventional macroscale cell-based assays;however,long fabrication times and high-cost specialized equipment limit the widespread adoption of microfluidic *** improvements in vat photopolymerization three-dimensional(3D)printing technologies such as liquid crystal display(LCD)printing offer rapid prototyping and a cost-effective solution to microfluidic *** information is available about how 3D printing parameters and resin cytocompatibility impact the performance of 3D-printed molds for the fabrication of polydimethylsiloxane(PDMS)-based microfluidic platforms for cellular *** a low-cost,commercially available LCD-based 3D printer,we assessed the cytocompatibility of several resins,optimized fabrication parameters,and characterized the minimum feature *** evaluated the response to both cytotoxic chemotherapy and targeted kinase therapies in microfluidic devices fabricated using our 3D-printed molds and demonstrated the establishment of flow-based concentration ***,we monitored real-time cancer cell and fibroblast migration in a 3D matrix environment that was dependent on environmental *** results demonstrate how vat photopolymerization LCD-based fabrication can accelerate the prototyping of microfluidic platforms with increased accessibility and resolution for PDMS-based cell culture assays.

关键词： invasion printing culture

Multifunctional glycolipids as multi-targeting therapeutics for neural regeneration

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Neural Regeneration Research 2024年第4期19卷 707-708页

作者： Yutaka Itokazu department of Neuroscience and regenerative Medicine Medical College of GeorgiaAugusta UniversityAugustaGAUSA

Many patients with neurodegenerative diseases,such as Alzheimer’s(AD)and Parkinson’s(PD)diseases suffer from disease progression without any satisfying clinical intervention,likely due to our lack of knowledge on how normal aging impacts the pathogenic mechanisms of these debilitating diseases.A growing body of literature has emerged in recent years that clearly demonstrates the involvement of glycolipids in the protein-oligomerization of neurodegenerative *** hypothesize that changes in glycolipids composition are a common mechanism underlying the shift from healthy brain aging to the neuropathological processes of neurodegenerative diseases.

关键词： lipids involvement diseases

Mesoporous bioactive glass composition effects on degradation and bioactivity

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Bioactive Materials 2021年第7期6卷 1921-1931页

作者： M.Schumacher P.Habibovic S.van Rijt department of Instructive Biomaterials Engineering MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht Universitythe Netherlands

Mesoporous bioactive glasses(MBGs)are promising materials for regenerative medicine,due to their favorable properties including bioactivity and *** key properties,but also their surface area,pore structure and pore volume are strongly dependent on synthesis parameters and glass ***,to date no systematic study on MBG properties covering a broad range of possible compositions ***,24 MBG compositions in the SiO_(2)-CaO-P_(2)O_(5) system were synthesized by varying SiO_(2)(60-90 mol%),CaO and P_(2)O_(5) content(both 0 to 40 mol-%),while other synthesis parameters were kept *** characteristics,degradability and bioactivity were *** results showed that,within the tested range of compositions,mesopore formation required a molar SiO_(2) content above 60%but was independent of CaO and P_(2)O_(5) *** mesopore size did not depend on glass stoichiometry,mesopore arrangement was influenced by the SiO_(2) *** surface area and pore volume were slightly altered by the SiO_(2) *** materials were degradable;however,degradation as well as bioactivity,*** ability to form a CaP mineral on the surface,depended on *** differences were found in early surface reactions in simulated body fluid:where some MBGs induced direct hydroxyapatite crystallization,high release of calcium in others resulted in calcite *** summary,degradation and bioactivity,both key parameters of MBGs,can be controlled by glass stoichiometry over a broad range while leaving the unique structural parameters of MBGs relatively *** allows targeted selection of material compositions for specific regenerative medicine applications.

关键词： bioactivity bioactive arrangement

Optimizing bone marrow harvesting sites for enhanced mesenchymal stem cell yield and efficacy in knee osteoarthritis treatment

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World Journal of Methodology 2025年第2期15卷 92-107页

作者： Arulkumar Nallakumarasamy Sandeep Shrivastava Ravi Velamoor Rangarajan Naveen Jeyaraman Avinash Gandi Devadas Swaminathan Ramasubramanian Madhan Jeyaraman department of Orthopaedics Datta Meghe Institute of Higher Education and ResearchWardha 442004MaharashtraIndia department of regenerative Medicine Mother Cell Regenerative CentreTiruchirappalli 620017Tamil NaduIndia department of Orthopaedics Government Medical CollegeOmandurar Government EstateChennai 600002Tamil NaduIndia department of Orthopaedics ACS Medical College and HospitalDr MGR Educational and Research InstituteChennai 600077Tamil NaduIndia

Knee osteoarthritis(OA)is a debilitating condition with limited long-term treatment *** therapeutic potential of mesenchymal stem cells(MSCs),particularly those derived from bone marrow aspirate concentrate,has garnered attention for cartilage repair in *** the iliac crest is the traditional site for bone marrow harvesting(BMH),associated morbidity has prompted the exploration of alternative sites such as the proximal tibia,distal femur,and proximal *** paper reviews the impact of different harvesting sites on mesenchymal stem cell(MSC)yield,viability,and regenerative potential,emphasizing their relevance in knee OA *** iliac crest consistently offers the highest MSC yield,but alternative sites within the surgical field of knee procedures offer comparable MSC characteristics with reduced *** integration of harvesting techniques into existing knee surgeries,such as total knee arthroplasty,provides a less invasive approach while maintaining thera-peutic ***,variability in MSC yield from these alternative sites underscores the need for further research to standardize techniques and optimize clinical *** directions include large-scale comparative studies,advanced characterization of MSCs,and the development of personalized harvesting ***,the findings suggest that optimizing the site of BMH can significantly influence the quality of MSC-based therapies for knee OA,enhancing their clinical utility and patient outcomes.

关键词： Knee Osteoarthritis Mesenchymal stem cells Bone marrow harvest regenerative medicine

A novel approach in preventing vascular leakage and angiogenesis in wet age-related macular degeneration

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Neural Regeneration Research 2022年第8期17卷 1751-1752页

作者： Kaori H.Yamada department of Pharmacology and regenerative Medicine Department of Ophthalmology and Visual SciencesUniversity of Illinois College of MedicineChicagoILUSA

Vascular leakage and angiogenesis in diseases:The vascular system carries blood throughout the body to supply oxygen and nutrients to tissues and remove waste from *** of the vascular system worsens many *** this perspective,vascular function in blinding eye diseases,current therapies,and our developing approach will be *** blood vessels are composed of the monolayer of the endothelial cells,and the outer layer made by pericytes and smooth muscle cells provide structural support and shape to the *** monolayers function as a *** barrier function of endothelial monolayers controls plasma protein uptake and the infiltration of immune cells by regulating adherence of cell-cell *** leakage causes unwanted plasma leak,accumulation of immune cells,and tissue inflammation.

关键词： degeneration approach remove