检索结果-南通市图书馆

Chinese Journal of cancer research 1992年第3期4卷 13-17页

作者：周筱梅顾健人陈渊卿蒋惠秋钱连芳徐国威 David Shafritz department of biochemistry and Molecular Biology Shanghai Cancer Institute Liver Cancer Research Center Albert Einstein University New York USA

This is first report about the simultaneous over-expression of both Insulin-like growth factor (IGF- I ) and its receptor (IGF- I R) at mRNA level in human primary hepatic cancer (PHC). In 10 PHC samples from China, IGF-I and IGF- I R were both over-expressed, whereas only a background signal was detected in normal liver. In 5 pairs of PHC and its non- tumorous adjacent liver tissues from South Africa, IGF- I and IGF- I R were also over-expressed in PHC. mRNA expression of IGF- I in all 5 cases and IGF- I R in 4 of 5 cases were higher in cancer than non- tumorous adjacent liver tissues. These results strongly implicate that an autocrine and/ or paracrine mechanism might be Involved in formation and progression of PHC.

关键词： AND IGF SIMULTANEOUS OVER-EXPRESSION OF INSULIN-LIKE GROWTH FACTOR RECEPTOR GENES IN HUMAN PRIMARY cancer-IMPLICATION OF AUTOCRINE AND PARACRINE MECHANISM IN AUTONOMOUS GROWTH OF HEPATIC cancer

ENZYMO-ROCKET ELECTROPHORETIC ASSAY AND CROSSED AFFINITY ENZYMOIMMUNOELECTROPHORESIS AND ITS APLICATION IN DIAGNOSIS OF PLIMARY LIVER cancer

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of cancer research 1995年第2期7卷 108-114页

作者：黄铧廖兆全黄济群黄炜 department of biochemistry Guangzhou Medical College Guangzhou cancer Institute Guangzhou Medical College China

A senitive and accurate quantitative method of alphafetoprotein(AFP), enzymo-rocket electrophoretic assay (EREA), was developed by introducing horseradish peroxidase-labeled anti-human AFP antibody into rocket *** lower limit of quantitation ty this method is about 10 ng/ml of *** dose-response curve covers a broader range of concentrations of AFP (10-4000ng/ml)than RIA(0-400 ng/ml) .The accuracy and precision of comparable to that of RIA(r=0.986).Serum AFP measured in 100 patients with primary liver cancer by this method,88% had levels over 25ng/*** crossed affinity enzymoimmunoelectrphoresis is a combination of lentil lectin(LCA)affinity electrophoresis and enzymo-rocket electrophoresis,it has been possible to separate the AFP into two variants,LCA-reactive(LCA-R)and LCA-nonreactive (LCA-N)*** advantages of this method are high sensitivity,rapid(6-7h),and can be effectively used to differentiate the primary liver cancer and benign liver disease.

关键词： Euzymo-rocket electrophoretic assay Crossed affinity enzymoimmunoelectrophoresis Plimary liver cancer Alpha-fetoprotein.

A new technique helps to uncover unknown peptides and disinfection by-products in water

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2016年第4期28卷 6-8页

作者： Susan D.Richardson Cristina Postigo department of Chemistry and biochemistry University of South Carolina department of Environmental Chemistry Institute for Environmental Assessment and Water Research-Spanish National Research Council(IDAEA-CSIC)

Environmental water samples can be extremely complex,with potentially thousands of molecules that can derive from natural organic matter（NOM）and thousands that derive from anthropogenic *** complex as these samples are,drinking water can be even more *** to disinfectants that are used to treat drinking water（e.g.,chlorine,chloramines,

关键词： Disinfection by-products(DBPs) Drinking water Halogenated peptides Liquid chromatography Natural organic matter(NOM) Non-targeted analysis Tandem mass spectrometry Water contaminants

Bioactive polyphenolic compounds, water-soluble vitamins, in vitro anti-inflammatory, anti-diabetic and free radical scavenging properties of underutilized alternate crop Amaranthus spinosus L. from Gangetic plain of West Bengal

在线全文

学校读者我要写书评

暂无评论

Food Bioscience 2022年第6期50卷 799-813页

作者： Agnideepa Kar Soumen Bhattacharjee Plant Physiology&biochemistry research laboratory UGC Centre for Advanced StudyDepartment of BotanyThe University of BurdwanBurdwanWest BengalIndia UGC centre for Advanced Study Department of BotanyThe University of BurdwanBurdwanWest BengalIndia

Studies associated with validation of folklore and ethnomedicinal claim of utilization of different plant parts of ‘ spiny amaranth’ (Amaranthus spinosus L.) against degenerative diseases with chemical data, particularly the bioactive polyphenolic compounds and associated anti-degenerative properties are scanty and is the purpose of the present investigations. RP-HPLC coupled photodiode assay was employed for the quantification of twenty-one health promoting bioactive polyphenolic antioxidants derived from phenylpropanoid pathway from different plant parts of Amaranthus spinosus L. for validating organoleptic traditional claims as well as current trends in practice of this underutilized medicinal herb. Further, important water-soluble vitamins [vitamin B1, B2, B3, B6, B9] present in different plant parts were extracted and estimated through RP-HPLC for validating the claim of their functional food properties. The antioxidant-based functional food property of different plant parts of Amaranthus spinosus were corroborated from the data of in vitro free radical scavenging, metal-chelating, reducing, superoxide radical scavenging, hydroxyl radical scavenging, anti-protein coagulation, anti-lipid peroxidation and antidiabetic properties. When compared, a positive correlation in accumulation of polyphenolic compounds and water-soluble vitamins tested with antioxidant attributes were noticed for different plant parts, strongly substantiating their phytonutrient properties. Taken as a whole, the present work provides data on organ-specific bioavailability of bioactive polyphenolic antioxidant compounds, water soluble B- vitamins and their functional attributes for validating traditional claims as well as existing trends in consumption of this underutilized medicinal vegetable as low-cost natural antioxidant supplement and functional food, which may encourage their effective exploration and utilization.

关键词： Spiny amaranth Bioactive polyphenolic compounds B-Vitamins Antioxidant and anti-inflammatory properties Ethnomedicinal use Functional food

Micropropagation protocol for Salvadora oleoides

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Forestry research 2019年第1期30卷 87-93页

作者： Ravindra Kumar Raoof Ahmad Najar Kunj Bihari Gupta Ram Gopal Saini department of biochemistry and Microbial Sciences School of Basic and Applied Sciences Central University of Punjab department of Plant Sciences School of Basic and Applied Sciences Central University of Punjab centre for Interdisciplinary Biomedical research Adesh University

Salvadora oleoides Decne. is a pharmaceutically important plant. Owing to poor seed formation, viability and, germination, and to anthropogenic disturbances, this species is on the verge of extinction. A reproducible micropropagation protocol to increase the population through tissue culture has been standardized and the results are reported here. Callus tissues were initiated from young leaves and stem explants. Leaf calluses proliferated with 1.5 mg/L BAP and 0.9 mg/L 2, 4-D with additives and continuous slow proliferation up to 15 weeks on 0.5 mg/L BAP and additives with 200 mg/L activated *** shoot initiation took place from stem node explants after 12 days; 4–5 shoots per node were produced in 30 days. Shoot clumps elongated and grew further on MS media supplemented with 2 mg/L BAP, 0.2 mg/L NAA and additives, which generated 20–23 shoots. The elongated shoots induced tap roots with 4 mg/L NAA and200 mg/L activated charcoal in 12 days. In vitro raised plants produced secondary roots when transferred to pots containing vermiculite maintained at 28–35 ℃. The plantlets successfully acclimatised in pots containing soil in natural conditions.

关键词： Salvadora oleoides Endangered species Micropropagation Explants Calluses Acclimatize

Elevated peripheral blood lymphocyteto-monocyte ratio predicts a favorable prognosis in the patients with metastatic nasopharyngeal carcinoma

在线全文

学校读者我要写书评

暂无评论

癌症 2015年第6期34卷 237-246页

作者： Rou Jiang Xiu-Yu Cai Zhong-Han Yang Yue Yan Xiong Zou Ling Guo Rui Sun Dong-Hua Luo Qiu-Yan Chen Pei-Yu Huang Yan-Qun Xiang Xing Lu Lin Wang Wei-Xiong Xia Hai-Qiang Mai Ming-Yuan Chen Sun Yat-sen University cancer Center State Key Laboratory of Oncology in South China Collaborative Innovation Center for cancer Medicine 4. department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer Center 5. Department of Cancer Prevention 6. Department of Medical Oncology Sun Yat-sen University Cancer Center department of biochemistry Zhongshan School of MedicineSun Yat-sen University

Introduction:Patients with metastatic nasopharyngeal carcinoma(NPC)have variable survival *** have previously shown that an elevated peripheral blood lymphocyte-to-monocyte ratio(LMR)is associated with an increased metastatic risk in patients with primary *** present study aimed to investigate the prognostic value of pretreatment LMR in a large cohort of metastatic NPC ***:Clinical data of 672 patients with metastatic NPC diagnosed between January 2003 and December2009 were *** peripheral lymphocyte and monocyte counts were retrieved,and LMR was *** operating characteristic(ROC)curve analysis and univariate and multivariate COX proportional hazards analyses were performed to evaluate the association of LMR with overall survival(OS).Results:Univariate analysis revealed that an elevated absolute lymphocyte count(≥1.390×109/L)and LMR(≥2.475)as well as a decreased monocyte count(<0.665×109/L)were significantly associated with prolonged *** Cox proportional hazard analysis showed that LMR(hazard ratio[HR]=0.50,95%confidence interval[CI]=0.41–0.60,P<0.001),absolute lymphocyte count(HR=0.77,95%CI=0.64–0.93,P=0.007),and monocyte count(HR=1.98,95%CI=1.63–2.41,P<0.001)were independent prognostic *** stratification analyses,only LMR remained a significant predictor of ***:We identified pretreatment LMR as an independent prognostic factor for patients with metastatic *** validation of our findings is needed.

关键词： Metastatic nasopharyngeal carcinoma Lymphocyte count Monocyte count Lymphocyte-to-monocyte ratio Overall survival Prognosis

Enhancement of Urinary Elimination of 3-Bromobenzanthrone Metabolites by Oral Supplementation of Ascorbic Acid in Guinea Pigs

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2004年第4期17卷 390-396页

作者： RAVINDRA P. SINGH RAJ KHANNA SUBHASH K. KHANNA MUKUL DAS department of biochemistry Lucknow University Lucknow-226001Department of Biochemistry Lucknow University Lucknow-226001Food Toxicology Laboratory Industrial Toxicology Research Centre Postal Box No. 80 M. G. Marg Lucknow-226 001 IndiaFood Toxicology Laboratory Industrial Toxicology Research Centre Postal Box No. 80 M. G. Marg Lucknow-226 001 India

Objective 3-Bromobenzanthrone (3-BBA), an anthraquinone intermediate dye, is extensively used in textile industry. Since, our prior studies have shown that 3-BBA caused significant depletion of ascorbic acid (AsA) levels, the effect of exogenous supplementation of AsA on the urinary elimination of 3-BBA metabolites was investigated. Method Guinea pigs were treated with single oral dose of 3-BBA (50 mg/kg b. wt.) in groundnut oil while another group was treated with single oral dose of 3-BBA (50 mg/kg b. wt.) along with 3 day prior and post oral supplementation of AsA. Control groups were either treated with groundnut oil or AsA alone. Urine from individual animals was collected, extracted and analysed on HPTLC. Results The highest elimination of 3-BBA (75 mg) was found to be in 0-24 h urine fraction which decreased to 18 mg and 5 mg in the two subsequent 24 hourly fractions of urine. Exogenous supplementation of AsA increased the total urinary elimination of 3-BBA by almost 77%. A total of 10 fluorescent metabolites excluding the parent compound were eliminated in the urine of guinea pigs treated with 3-BBA. Densitometric scanning of chromatogram showed different peaks at Rf 0.18, 0.22, 0.27, 0.34, 0.40, 0.48, 0.56, 0.66, 0.72, 0.80, and 0.95 which were eliminated and marked as urinary metabolite 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11 respectively. AsA not only significantly enhanced the elimination of 3-BBA metabolites but also modified the pattern of metabolites drastically in 0-6 h, 6-24 h and 24-48 h urine fractions. Conclusion These results indicate that AsA may be useful in protecting the toxicity of 3-BBA by fascilitating the urinary metabolite(s) excretion of 3-BBA.

关键词： Ascorbic acid 3-Bromobenzanthrone Metabolites High performance thin layer chromatography

The complex roles of m^(6)A modifications in neural stem cell proliferation, differentiation, and self-renewal and implications for memory and neurodegenerative diseases

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration research 2025年第6期20卷 1582-1598页

作者： Yanxi Li Jing Xue Yuejia Ma Ke Ye Xue Zhao Fangliang Ge Feifei Zheng Lulu Liu Xu Gao Dayong Wang Qing Xia department of biochemistry and Molecular Biology College of Basic Medical SciencesHarbin Medical UniversityHarbinHeilongjiang ProvinceChina College of Basic Medical Sciences Harbin Medical UniversityHarbinHeilongjiang ProvinceChina Basic Medical Institute Heilongjiang Academy of Medical SciencesHarbinHeilongjiang ProvinceChina Key Laboratory of Heilongjiang Province for Genetically Modified Animals Harbin Medical UniversityHarbinHeilongjiang ProvinceChina Translational Medicine research and Cooperation Center of Northern China Heilongjiang Academy of Medical SciencesHarbinHeilongjiang ProvinceChina Dongzhimen Hospital Beijing University of Chinese MedicineBeijingChina

N6-methyladenosine(m^(6)A), the most prevalent and conserved RNA modification in eukaryotic cells, profoundly influences virtually all aspects of mRNA metabolism. mRNA plays crucial roles in neural stem cell genesis and neural regeneration, where it is highly concentrated and actively involved in these processes. Changes in m^(6)A modification levels and the expression levels of related enzymatic proteins can lead to neurological dysfunction and contribute to the development of neurological diseases. Furthermore, the proliferation and differentiation of neural stem cells, as well as nerve regeneration, are intimately linked to memory function and neurodegenerative diseases. This paper presents a comprehensive review of the roles of m^(6)A in neural stem cell proliferation, differentiation, and self-renewal, as well as its implications in memory and neurodegenerative diseases. m^(6)A has demonstrated divergent effects on the proliferation and differentiation of neural stem cells. These observed contradictions may arise from the time-specific nature of m^(6)A and its differential impact on neural stem cells across various stages of development. Similarly, the diverse effects of m^(6)A on distinct types of memory could be attributed to the involvement of specific brain regions in memory formation and recall. Inconsistencies in m^(6)A levels across different models of neurodegenerative disease, particularly Alzheimer's disease and Parkinson's disease, suggest that these disparities are linked to variations in the affected brain regions. Notably, the opposing changes in m^(6)A levels observed in Parkinson's disease models exposed to manganese compared to normal Parkinson's disease models further underscore the complexity of m^(6)A's role in neurodegenerative processes. The roles of m^(6)A in neural stem cell proliferation, differentiation, and self-renewal, and its implications in memory and neurodegenerative diseases, appear contradictory. These inconsistencies may be a

关键词： Alzheimer's disease cell self-renewal central nervous system memory microglia nerve regeneration neurodegenerative diseases neurogenesis RNA methylation

Profiling neuroprotective potential of trehalose in animal models of neurodegenerative diseases:a systematic review

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration research 2023年第6期18卷 1179-1185页

作者： Kah Hui Yap Shahrul Azmin Suzana Makpol Hanafi Ahmad Damanhuri Muzaimi Mustapha Jemaima Che Hamzah Norlinah Mohamed Ibrahim department of Medicine UKM Medical CentreKuala LumpurMalaysia department of biochemistry UKM Medical CentreKuala LumpurMalaysia department of Neurosciences School of Medical SciencesUniversiti Sains MalaysiaKelantanMalaysia department of Ophthalmology UKM Medical CentreKuala LumpurMalaysia

Trehalose,a unique nonreducing crystalline disaccharide,is a potential disease-modifying treatment for neurodegenerative diseases associated with protein misfolding and aggregation due to aging,intrinsic mutations,or autophagy *** systematic review summarizes the effects of trehalose on its underlying mechanisms in animal models of selected neurodegenerative disorders(tau pathology,synucleinopathy,polyglutamine tract,and motor neuron diseases).All animal studies on neurodegenerative diseases treated with trehalose published in Medline(accessed via EBSCOhost)and Scopus were *** the 2259 studies screened,29 met the eligibility *** to the SYstematic Review Center for Laboratory Animal Experiment(SYRCLE)risk of bias tool,we reported 22 out of 29 studies with a high risk of *** present findings support the purported role of trehalose in autophagic flux and protein *** review identified several other lesser-known pathways,including modifying amyloid precursor protein processing,inhibition of reactive gliosis,the integrity of the blood-brain barrier,activation of growth factors,upregulation of the downstream antioxidant signaling pathway,and protection against mitochondrial *** absence of adverse events and improvements in the outcome parameters were observed in some studies,which supports the transition to human clinical *** is possible to conclude that trehalose exerts its neuroprotective effects through both direct and indirect ***,heterogeneous methodologies and outcome measures across the studies rendered it impossible to derive a definitive *** studies on trehalose would need to clarify three important questions:1)bioavailability with oral administration,2)optimal time window to confer neuroprotective benefits,and 3)optimal dosage to confer neuroprotection.

关键词： amyotrophic lateral sclerosis autophagy neurodegenerative disease neuroinflammation polyglutamine tract protein refolding spinocerebellar ataxia synucleinopathy tau pathology trehalose

Absolute copy number differences of Y chromosomal genes between crossbred (Bos taurus×Bos indicus) and Indicine bulls

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2013年第4期4卷 257-263页

作者： Ayan Mukherjee Gulshan Dass Jagan Mohanarao G Moloya Gohain Biswajit Brahma Tirtha Kumar Datta Sachinandan De Animal Genomics Lab Animal Biotechnology Centre National Dairy Research Institute department of Veterinary biochemistry College of Veterinary Sciences & Animal Husbandry Central Agricultural University

Background： The Y chromosome in mammal is paternally inherited and harbors genes related to male fertility and spermatogenesis. The unique intra-chromosomal recombination pattern of Y chromosome and morphological difference of this chromosome between Bos taurus and Bos indicus make it an ideal model for studying structural variation, especially in crossbred （Bos taurus x Bos indicus） bulls. Copy Number Variation （CNV） is a type of genomic structural variation that gives information complementary to SNP data. The purpose of this study was to find out copy number differences of four Y chromosomal spermatogenesis-related candidate genes in genomic DNA of crossbred and purebred Indicine bulls. Result： Four Y chromosomal candidate genes of spermatogenesis namely, sex determining gene on Ychromosome （SRY）, DEAD box po/ypeptide 3-Y chromosome （DDX3 Y）, Ubiquidn specific peptidase 9, Y-linked （ usPgY）, testis-specific protein on Y chromosome （TSPY） were evaluated. Absolute copy numbers of Y chromosomal genes were determined by standard curve-based quantitative real time PCR. Copy numbers of SRYand TSPYgenes per unit amount of genomic DNA are higher in crossbred than Indicine bulls. However, no difference was observed in DDX3Yand usPgYgene copy numbers between two groups. Conclusion： The present study demonstrates that the structural organization of Y chromosomes differs between crossbred and Indicine bulls which are reproductively healthy as observed from analysis of semen attributes. The absolute copy numbers of SRY and TSPY genes in unit mass of genomic DNA of crossbred bulls are significantly higher than Indicine bulls. No alteration in absolute copies ofDDX3Yand usPgYgene was found between the genome of crossbred and Indicine bulls. This study suggests that the DDX3Yand USPgYare likely to be single copy genes in the genome of crossbred and Indicine bulls and variation in Y chromosome length between crossbred and Indicine bulls may be due to the copy number variation of

关键词： Absolute copy SRY DDX3Y usPgY TSPY Crossbred