Enhancement of Urinary Elimination of 3-Bromobenzanthrone Metabolites by Oral Supplementation of Ascorbic Acid in Guinea Pigs

作     者：RAVINDRA P. SINGH RAJ KHANNA SUBHASH K. KHANNA MUKUL DAS RAVINDRA P. SINGH*, RAJ KHANNA*, SUBHASH K. KHANNA**, AND MUKUL DAS**,# *Department of Biochemistry, Lucknow University Lucknow-226001;**Food Toxicology Laboratory, Industrial Toxicology Research Centre, Postal Box No. 80, M. G. Marg, Lucknow-226 001, India

作者机构：Department of Biochemistry Lucknow University Lucknow-226001Department of Biochemistry Lucknow University Lucknow-226001Food Toxicology Laboratory Industrial Toxicology Research Centre Postal Box No. 80 M. G. Marg Lucknow-226 001 IndiaFood Toxicology Laboratory Industrial Toxicology Research Centre Postal Box No. 80 M. G. Marg Lucknow-226 001 India 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2004年第17卷第4期

页      面：390-396页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 0822[工学-轻工技术与工程] 

主　　题：Ascorbic acid 3-Bromobenzanthrone Metabolites High performance thin layer chromatography 

摘      要：Objective 3-Bromobenzanthrone (3-BBA), an anthraquinone intermediate dye, is extensively used in textile industry. Since, our prior studies have shown that 3-BBA caused significant depletion of ascorbic acid (AsA) levels, the effect of exogenous supplementation of AsA on the urinary elimination of 3-BBA metabolites was investigated. Method Guinea pigs were treated with single oral dose of 3-BBA (50 mg/kg b. wt.) in groundnut oil while another group was treated with single oral dose of 3-BBA (50 mg/kg b. wt.) along with 3 day prior and post oral supplementation of AsA. Control groups were either treated with groundnut oil or AsA alone. Urine from individual animals was collected, extracted and analysed on HPTLC. Results The highest elimination of 3-BBA (75 mg) was found to be in 0-24 h urine fraction which decreased to 18 mg and 5 mg in the two subsequent 24 hourly fractions of urine. Exogenous supplementation of AsA increased the total urinary elimination of 3-BBA by almost 77%. A total of 10 fluorescent metabolites excluding the parent compound were eliminated in the urine of guinea pigs treated with 3-BBA. Densitometric scanning of chromatogram showed different peaks at Rf 0.18, 0.22, 0.27, 0.34, 0.40, 0.48, 0.56, 0.66, 0.72, 0.80, and 0.95 which were eliminated and marked as urinary metabolite 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11 respectively. AsA not only significantly enhanced the elimination of 3-BBA metabolites but also modified the pattern of metabolites drastically in 0-6 h, 6-24 h and 24-48 h urine fractions. Conclusion These results indicate that AsA may be useful in protecting the toxicity of 3-BBA by fascilitating the urinary metabolite(s) excretion of 3-BBA.