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Isolation and Characterization of Mercury Resistant Trichoderma Strains from Soil with High Levels of Mercury and Its Effects on Arabidopsis thaliana Mercury Uptake

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Advances in Microbiology 2018年第7期8卷 600-613页

作者： JoséLuis Hernández-Flores JoséGerardo Bernal Melo Andrés Cruz Hernández Miguel A.Ramos López Carlos Saldana Gutiérrez Sergio Romero Gomez Victor Pérez Moreno Rosa P.Calvillo Medina JoséHumberto Valenzuela Soto Sergio Pacheco Hernández George H.Jones Sergio Casas Flores Juan Campos-Guillen Laboratorio de Bioseguridad y Análisis de Riesgo Departamento de Ingeniería Genética de PlantasCentro de Investigación y de Estudios Avanzados del IPNIrapuatoGto.México Facultad de Química Universidad Autónoma de QuerétaroCerro de las Campanas s/nQuerétaroQro.México Escuela de Agronomía Universidad De La Salle Bajíocampus Campestre.LeónGto.México Facultad de Ciencias Naturales Universidad Autónoma de QuerétaroAv.De las Ciencias S/NQuerétaroQro.México Centro de Investigación en Química Aplicada Blvd.Enrique Reyna Hermosillo No.140SaltilloCoahuilaMéxico department of Biology Emory UniversityAtlantaGeorgiaUSA IPICYT División de Biología Molecular.Camino a la presa San JoséNo.2055Colonia Lomas 4a sección.C.P.San Luis PotosíMexico

Traditional mining activities are usually correlated with high levels of soil pollution, which is a major environmental concern. Extensive mining activities have taken place in the San Joaquin region in the State of Querétaro, México resulting in high levels of mercury soil pollution (up to 1532 ± 300 mg/kg). We isolated mercury-resistant fungal strains from the San Joaquin region soils and identified them through morphologic characteristics and ITS rDNA region sequence analysis. We determined that fungi isolated belong to the genus Trichoderma. All the isolates selected showed the ability to catalyze the volatilization of Hg. For air sampling, an active sampling device was constructed and using acid KMnO4 as an absorbent, the concentration of mercury in solution was determined through the cold vapor atomic absorption method. The results show mercury volatilization from the fungal species assay, with a maximum of 213.04 ± 32.6 μg/m3 while mycelium accumulation ranged from less than 17.5 ± 2.9 to 20.0 ± 3.4 μg/g. The fungal isolates were also evaluated for their ability to reduce mercury uptake in Arabidopsis thaliana. These observations suggest the utility of Trichoderma for the mobilization of mercury in those contaminated soils.

关键词： Volatilization Mercury Trichoderma Soil Bioremediation

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Synthesis of Amine Terminated Pegylated Iron Oxide Nanoparticles for Prospective Astrocytoma Resection Grade Improvement

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材料科学与工程（中英文A版） 2019年第1期9卷 25-37页

作者： Isaias Eduardo Hernández-Verdin María Luisa Martínez Alejandro Miranda-González Mónica Alicia Sierra del Río Oracio Serrano-Torres Mario Avila-Rodríguez Experimental neuro-oncology Groupe Hospitalier Pitié-SalpêtrièreParis 75013France departamento de Ciencias e ingeniería Universidad Iberoamericana LeónLeón 37238Mexico departamento de Neurociencias Hospital Regional de Alta Especialidad del BajíoLeón 37660Mexico departamento de Química División de Ciencias Naturales y ExactasCampus GuanajuatoUniversidad de GuanajuatoGuanajuato 36040Mexico

Having a survival rate to 5 years of only 3%,Glioblastoma’s(GBM)main treatment is surgical *** oxide nanoparticles have been proved to be a magnetic resonance imaging contrast agent and,if synthesized and tuned correctly,could be used to improve complete GBM *** this work monodisperse iron oxide nanoparticles were synthesized using thermal decomposition method,then a ligand exchange reaction with 3-aminopropyl trimethoxysilane(APS)was performed,following Pegylation of the particles using dicarboxylic acid PEG(PEG-diacid)and finally aminating with 2,2’-(ethylenedioxy)bis(ethylamine),last two by amide *** and DLS demonstrate monodispersity(log σdesired size range to penetrate the blood-brain barrier(BBB);FT-IR shows the reactions were executed correctly and finally stability in deionized water,0.07 M NaCl and PBS 1X,as a function of 0-30 days,was *** revealed the importance that the oleic acid/iron oleate molar ratio and the growth stage time represents for determining iron oxide nanoparticles’ size;as well as APS concentration and nucleation time influence on silica coating when performing the ligand exchange *** produced iron oxide nanoparticles exhibit stability and proper amine terminated groups which are needed to allow easy incorporation of Chlorotoxin,a 36-amino acid peptide that binds specifically to astrocytoma cells,and a fluorescent molecule,which enables real time visualization of the tumor during surgery.

关键词： Iron oxide nanoparticles silanization pegylation astrocytoma

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Functional Properties of Japanese Quail (Coturnix coturnix japonica) Eggs Proteins

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Journal of Food Science and Engineering 2020年第2期10卷 47-54页

作者： JoséFernando González Sánchez Haydee Hernández Unzón Esmeralda Mónica Peña-González departamento de Producción Agrícola y Animal.Universidad Autónoma Metropolitana Unidad Xochimilco.Ciudad de MéxicoC.P.04960México departamento de ingeniería Bioquímica Escuela Nacional de Ciencias Biológicas IPNCiudad de MéxicoC.P.11340México

The eggs of the domestic chicken(Gallus gallus domesticus)are the most consumed,but those of duck,goose,and quail are also consumed,to name a *** egg is considered as a food rich in proteins,minerals,and *** generally consist of three main components:shell,white,and yolk,these have several functional properties that are used in food and other *** functional properties of the egg white and yolk are the ability of foaming,gelling,and emulsifying,which can be dealt with according to storage *** objective of this work was to evaluate the functional properties of the components of the Japanese quail *** functional properties of eggs stored at room temperature for 1,10,20,30,40,50,and 60 days after laying were *** are analyzed using a Tukey paired comparison *** volume of the foam of the quail egg white did not change with storage time,the emulsion capacity was 193.8 mL of oil per gram of protein from the *** coagulation capacity requires a lower temperature to coagulate the quail egg proteins,due to the slower transformation of ovalbumin to its thermo-stable form(S-ovalbumin)as compared to that of chicken.

关键词： Quail eggs proteins functional properties

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预制基于三维打印的个性化、血管化组织工程骨修复恒河猴下颌骨缺损的实验研究

预制基于三维打印的个性化、血管化组织工程骨修复恒河猴下颌骨缺...

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第十四次中国口腔颌面外科学术会议

作者：周苗曹帅帅 Pedro Miranda 车月娟陈晓明李树祎耿远明杨晓彬钱立广州医科大学附属口腔医院口腔颌面外科 departamento de ingeniería Mecánica Energética y de los Materiales Universidad de Extremadura 中山大学孙逸仙纪念医院麻醉科广州医科大学生物医学工程系南方医科大学珠江医院口腔科杭州九源基因工程有限公司

研究目的:观察预制基于三维打印的个性化、血管化组织工程骨和支架原位植入修复恒河猴下颌骨缺损的效果。研究方法:恒河猴进行头颅CT扫描,建立下颌骨节段性缺损的模型,对其孔隙率、孔径大小设计后得到支架的STL文件;采用CAD/CAM制作个... 详细信息

研究目的:观察预制基于三维打印的个性化、血管化组织工程骨和支架原位植入修复恒河猴下颌骨缺损的效果。研究方法:恒河猴进行头颅CT扫描,建立下颌骨节段性缺损的模型,对其孔隙率、孔径大小设计后得到支架的STL文件;采用CAD/CAM制作个性化钛网,作为颌骨内固定装置。将文件导入3D打印机,分别采用低温快速成型和自动注浆技术制作PLGA/TCP和TCP支架,并将两种支架灭菌后复合6mg rhBMP-2植入恒河猴下颌骨节段性缺损。9只成年恒河猴,随机分组对其15个下颌骨节段性缺损进行修复,分为原位修复S-PLGA/TCP组(S-P/T,n=3)、S-TCP组(S-T,n=3)、SPLGA/TCP-BMP组(S-P/T-B,n=3)、和S-TCP-BMP组(S-T-B,n=3),与转移后的预制P-T-B(n=3)共5组。在下颌骨缺损修复术后的4、8、12周采用PET/CT检测支架密度值和18F-FDG摄取值。下颌骨修复术后12周取材,通过血管灌注观察支架的血管化情况,对下颌骨样本进行大体观察、放射学检查、Micro-CT、生物力学和硬组织切片、外形匹配度检查,观察下颌骨修复情况。研究结果:下颌骨缺损修复术后PET/CT显示P-T-B组、S-P/T-B组和S-T-B组均显示18F-FDG明显的浓聚,S-P/T-B组支架密度随着时间延长降低,P-T-B组和S-T-B组支架的密度上升。S-P/T、S-P/T-B、S-T、S-T-B和P-T-B组下颌骨修复12周后匹配度分别为8.2±0.1%、21.2±1.2%、94.7±2.0%、93.5±2.2%和96.0±2.0%。处死后大体观察、Micro-CT和组织学检查显示节段性下颌骨缺损可被转移的组织工程骨瓣P-T-B和原位植入S-T-B组成功修复,S-P/T-B组、S-P/T组和S-T组未能修复下颌骨缺损。血管灌注结果显示背部植入支架由胸背动脉供应,预制转瓣下颌骨侧可见转移的胸背动脉。力学测试结果显示P-P/T组、P-P/T-B组、P-T组抗压强度均比植入前降低,S-T-B组和P-T-B组支架取材时力学性能均比植入前升高,P-T-B组的力学性能显著高于S-T-B组(P<0.05)。研究结论:基于自动注浆技术打印的TCP支架可以预制个性化、血管化组织工程骨,其转移后成功修复恒河猴下颌骨缺损。

关键词：三维打印低温快速成型自动注浆预制组织工程骨下颌骨重建

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三维打印β-TCP颌骨修复支架的生物学评价

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口腔医学研究 2017年第7期33卷 712-716页

作者：曹帅帅周苗 Pedro Miranda 车月娟陈晓明李树祎耿远明杨晓彬广州医科大学附属口腔医院.广州口腔病研究所.口腔医学重点实验室广东广州510140 departamento de ingeniería Mecánica Energética y de los MaterialesUniversidad de Extremaduraspain999023 中山大学孙逸仙纪念医院麻醉科广东广州510120 广州医科大学生物医学工程系广东广州511436 南方医科大学珠江医院口腔科广东广州510280

目的:探讨三维打印β-磷酸三钙(β-Tricalcium Phosphate,β-TCP)颌骨修复支架的生物学特性及体内成骨作用。方法:采用自动注浆技术制作β-TCP支架,将前成骨细胞(MC35T3-E1)接种在支架上,扫描电镜(SEM)观察材料结构与细胞黏附,CCK-8法... 详细信息

目的:探讨三维打印β-磷酸三钙(β-Tricalcium Phosphate,β-TCP)颌骨修复支架的生物学特性及体内成骨作用。方法:采用自动注浆技术制作β-TCP支架,将前成骨细胞(MC35T3-E1)接种在支架上,扫描电镜(SEM)观察材料结构与细胞黏附,CCK-8法检测细胞增殖,ALP法检测碱性磷酸酶活性。将2种支架复合重组人骨形成蛋白-2(recombinant human bone morphogenetic protein-2,rhBMP-2)后植入大鼠体内,发泡法制作的β-TCP支架为对照组,6周后取材行组织学观察。结果:三维打印支架具有规则多孔的立体结构,适合细胞黏附,且增殖及分化能力均高于对照组(P<0.05)。组织学显示复合rhBMP-2后三维打印支架新骨生成量高于发泡法制作的β-TCP支架(P<0.05)。结论:三维打印TCP支架生物相容性良好,复合rhBMP-2后可异位成骨。

关键词：三维打印自动注浆技术 β-TCP rhBMP-2 骨修复

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Improving Compost Process Efficiency by Leachates Inoculation and Shredding of the Organic Fraction of Municipal Solid Waste at Bordo Poniente Composting Plant,Mexico City

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Journal of Environmental Science and Engineering(A) 2021年第5期10卷 177-183页

作者： Francisco Javier Barrón-Santos Ma.Eugenia Gutiérrez-Castillo Luis Raúl Tovar-Gálvez Ma.Teresa Núñez-Cardona Ricardo Estrada-Núñez Claudia Rodríguez Tapia Alfonso Espitia-Cabrera departamento de Posgrado Salud OcupacionalSeguridad e HigieneEscuela Nacional de Medicina y HomeopatíaInstituto Politécnico NacionalCiudad de México 07320México departamento de Biociencias e ingeniería Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y DesarrolloInstituto Politécnico NacionalCiudad de México 07340México departamento El Hombre y su Ambiente Universidad Autónoma Metropolitana XochimilcoCiudad de México 04960México Secretaría de Obras y Servicios Gobierno de la Ciudad de MéxicoCiudad de México 06000México Unidad Profesional Interdisciplinaria de Biotecnología Instituto Politécnico NacionalCiudad de México 07340México departamento de Energía Universidad Autónoma Metropolitana AzcapotzalcoCiudad de México 02200México

Management of MSW(Municipal Solid Waste)is a major downside in most of the biggest *** composting of the organic fraction of MSW is one of the oldest and simplest ways of organic waste *** is a self-heating biological conversion that generates appropriate finished merchandise such as soil conditioner or *** City generates about 12,500 ton/day of MSW,44%of it is food scraps and yard trimmings which are the OFMSW(Organic Fraction of Municipal Solid Waste),2,500 ton/day of it is composted at the BPCP(Bordo Poniente Composting Plant)yielding 500 ton/day of *** purpose of this study was to evaluate three treatments to accelerate the composting process,so eventually the city could increase the amount of the OFMSW at *** compared three different treatments;one of them showed a significant reduction in time of the composting process(p≤0.05)i.e.,it took less time to reach the thermophilic stage,maturity and stabilization *** was achieved at 35 days and 60 days to *** conclude that shredding the feedstock plus leachate inoculum addition at the beginning of the process,reduces the composting time in about 61%with respect to the time it takes at the BPCP,where lasted 90 days.

关键词： Composting organic fraction municipal solid waste inoculum leachates size particle

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development of a Construction Material for Indoor and Outdoor, Metakaolinite-Based Geopolymer, with Environmental Properties

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材料科学与工程（中英文A版） 2019年第4期9卷 131-142页

作者： M.Mondragón-Figueroa Héctor.R.Guzmán-Carrillo MiguelÁngel Rico JoséLuis.Reyez-Araiza Jorge.Pineda-Piñón Edgar J.López-Naranjo María C.Columba-Palomares Jóse.M.López-Romero Josè.Ramón Gasca-Tirado Alejandro.Manzano-Ramírez CINVESTAV-Querétaro Libramiento Norponiente#2000Fraccionamiento Real de JuriquillaQuerétaro 76230Qro.México Facultad de Química Universidad Autónoma de Querétaro centro de estudios Académicos sobre contaminación AmbientalQuerétaro 76010Qro.México DIPFI Facultad de IngenieríaUniversidad Autónoma de QuerétaroC.U.Cerro de las CampanasCentroQuerétaro 76010Qro.México Instituto Politécnico Nacional Centro de Investigación en Ciencia Aplicada y Tecnología AvanzadaUnidad QuerétaroCerro Blanco No.141Colinas del CimatarioQuerétaro76090Qro.México DIP-CUCEI Universidad de GuadalajaraJoséGuadalupe Zuno#48Los BelenesZapopan 45100Jal.México Facultad de Farmacia Universidad Autónoma del Estado de MorelosAv.Universidad#1001Cuernavaca 62209Mor.México departamento de ingeniería Universidad de GuanajuatoGuanajuato 36000México

Environmental problems that came from the human activity have many facets,since pollution of the atmosphere arises from vehicles,industrial emissions while pollution of water could be from organic compounds,pesticides *** can cause serious health effects,such as respiratory diseases,including asthma and lung ***,in the present work,a kinetic study on the effective adsorption and photo degradation of methylene blue(MB)dye,under ultraviolet A(UVA)irradiation of an alkali activated inorganic polymer(geopolymer)with homogeneously dispersed titanium dioxide(TiO2)micro-particles is *** addition,antimicrobial testing of the alkali activated TiO2 material was performed showing a bacteriostatic effect.

关键词： Heterogeneous photocatalysis semiconductor alkali activated bacteriostatic effect

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