Synthesis of Amine Terminated Pegylated Iron Oxide Nanoparticles for Prospective Astrocytoma Resection Grade Improvement

作     者：Isaias Eduardo Hernández-Verdin María Luisa Martínez Alejandro Miranda-González Mónica Alicia Sierra del Río Oracio Serrano-Torres Mario Avila-Rodríguez 

作者机构：Experimental neuro-oncologyGroupe Hospitalier Pitié-SalpêtrièreParis 75013France Departamento de Ciencias e IngenieríaUniversidad Iberoamericana LeónLeón 37238Mexico Departamento de NeurocienciasHospital Regional de Alta Especialidad del BajíoLeón 37660Mexico Departamento de QuímicaDivisión de Ciencias Naturales y ExactasCampus GuanajuatoUniversidad de GuanajuatoGuanajuato 36040Mexico 

出 版 物：《材料科学与工程（中英文A版）》 (Journal of Materials Science and Engineering A)

年 卷 期：2019年第9卷第1期

页      面：25-37页

学科分类：08[工学] 

主　　题：Iron oxide nanoparticles silanization pegylation astrocytoma 

摘      要：Having a survival rate to 5 years of only 3%,Glioblastoma’s(GBM)main treatment is surgical *** oxide nanoparticles have been proved to be a magnetic resonance imaging contrast agent and,if synthesized and tuned correctly,could be used to improve complete GBM *** this work monodisperse iron oxide nanoparticles were synthesized using thermal decomposition method,then a ligand exchange reaction with 3-aminopropyl trimethoxysilane(APS)was performed,following Pegylation of the particles using dicarboxylic acid PEG(PEG-diacid)and finally aminating with 2,2’-(ethylenedioxy)bis(ethylamine),last two by amide *** and DLS demonstrate monodispersity(log σ0.2)and desired size range to penetrate the blood-brain barrier(BBB);FT-IR shows the reactions were executed correctly and finally stability in deionized water,0.07 M NaCl and PBS 1X,as a function of 0-30 days,was *** revealed the importance that the oleic acid/iron oleate molar ratio and the growth stage time represents for determining iron oxide nanoparticles’ size;as well as APS concentration and nucleation time influence on silica coating when performing the ligand exchange *** produced iron oxide nanoparticles exhibit stability and proper amine terminated groups which are needed to allow easy incorporation of Chlorotoxin,a 36-amino acid peptide that binds specifically to astrocytoma cells,and a fluorescent molecule,which enables real time visualization of the tumor during surgery.