Isolation and Characterization of Mercury Resistant Trichoderma Strains from Soil with High Levels of Mercury and Its Effects on Arabidopsis thaliana Mercury Uptake

作     者：JoséLuis Hernández-Flores JoséGerardo Bernal Melo Andrés Cruz Hernández Miguel A.Ramos López Carlos Saldana Gutiérrez Sergio Romero Gomez Victor Pérez Moreno Rosa P.Calvillo Medina JoséHumberto Valenzuela Soto Sergio Pacheco Hernández George H.Jones Sergio Casas Flores Juan Campos-Guillen 

作者机构：Laboratorio de Bioseguridad y Análisis de RiesgoDepartamento de Ingeniería Genética de PlantasCentro de Investigación y de Estudios Avanzados del IPNIrapuatoGto.México Facultad de QuímicaUniversidad Autónoma de QuerétaroCerro de las Campanas s/nQuerétaroQro.México Escuela de AgronomíaUniversidad De La Salle Bajíocampus Campestre.LeónGto.México Facultad de Ciencias NaturalesUniversidad Autónoma de QuerétaroAv.De las Ciencias S/NQuerétaroQro.México Centro de Investigación en Química AplicadaBlvd.Enrique Reyna Hermosillo No.140SaltilloCoahuilaMéxico Department of BiologyEmory UniversityAtlantaGeorgiaUSA IPICYTDivisión de Biología Molecular.Camino a la presa San JoséNo.2055Colonia Lomas 4a sección.C.P.San Luis PotosíMexico 

出 版 物：《Advances in Microbiology》 (微生物学（英文）)

年 卷 期：2018年第8卷第7期

页      面：600-613页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：financed by the Universidad Autonoma de Queretaro(PFCE 2018 and FOPER 2017 to JCG)and a fellowship to G.B.M from CONACYT 

主　　题：Volatilization Mercury Trichoderma Soil Bioremediation 

摘      要：Traditional mining activities are usually correlated with high levels of soil pollution, which is a major environmental concern. Extensive mining activities have taken place in the San Joaquin region in the State of Querétaro, México resulting in high levels of mercury soil pollution (up to 1532 ± 300 mg/kg). We isolated mercury-resistant fungal strains from the San Joaquin region soils and identified them through morphologic characteristics and ITS rDNA region sequence analysis. We determined that fungi isolated belong to the genus Trichoderma. All the isolates selected showed the ability to catalyze the volatilization of Hg. For air sampling, an active sampling device was constructed and using acid KMnO4 as an absorbent, the concentration of mercury in solution was determined through the cold vapor atomic absorption method. The results show mercury volatilization from the fungal species assay, with a maximum of 213.04 ± 32.6 μg/m3 while mycelium accumulation ranged from less than 17.5 ± 2.9 to 20.0 ± 3.4 μg/g. The fungal isolates were also evaluated for their ability to reduce mercury uptake in Arabidopsis thaliana. These observations suggest the utility of Trichoderma for the mobilization of mercury in those contaminated soils.