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Hub genes associated with immune cell infiltration in breast cancer, identified through bioinformatic analyses of multiple datasets

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Cancer Biology & Medicine 2022年第9期19卷 1352-1374页

作者： Huanyu Zhao Ruoyu Dang Yipan Zhu Baijian Qu Yasra Sayyed Ying Wen Xicheng Liu Jianping Lin Luyuan Li State key laboratory of Medicinal Chemical Biology and college of pharmacy Tianjin Key Laboratory of Molecular Drug ResearchNankai UniversityTianjin 300350China Department of Physiology and Pathophysiology School of Basic Medical SciencesCapital Medical UniversityBeijing 100069China

Objective:The aim of this study was to identify hub genes associated with immune cell infiltration in breast cancer through bioinformatic analyses of multiple ***:Nonparametric(NOISeq)and robust rank aggregation-ranked parametric(EdgeR)methods were used to assess robust differentially expressed genes across multiple ***-protein interaction network,GO,KEGG enrichment,and subnetwork analyses were performed to identify immune-associated hub genes in breast *** cell infiltration was evaluated with the CIBERSORT,XCELL,and TIMER *** association between the hub gene-based risk signature and survival was determined through Kaplan–Meier survival analysis,multivariate Cox analysis,and a nomogram with external ***:We identified 163 robust differentially expressed genes in breast cancer through applying both nonparametric and parametric methods to multiple GEO(n=2,212)and TCGA(n=1,045)*** bioinformatic analyses further identified 10 hub genes:CXCL10,CXCL9,CXCL11,SPP1,POSTN,MMP9,DPT,COL1A1,ADAMDEC1,and *** 10 hub-gene-based risk signature significantly correlated with the prognosis of patients with breast ***,these hub genes were strongly associated with the extent of infiltration of CD4+T cells,CD8+T cells,neutrophils,macrophages,and myeloid dendritic cells into breast ***:Integrated analyses of multiple databases led to the discovery of 10 robust hub genes that together may serve as a risk factor characteristic of the immune microenvironment in breast cancer.

关键词： Bioinformatics breast cancer multi-datasets analysis immune cell infiltration survival analysis

Synthesis, Structure and Biological Activity of 2-[2-(4-Fluorobenzylidene)hydrazinyl]-4-(1-methyl-1H-indol-3-yl)thieno[3,2-d]pyrimidine

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Chinese Journal of Structural Chemistry 2019年第9期38卷 1530-1536页

作者： SHI Jian-Tao GONG Yi-Lin LI Jun WANG Yang CHEN Ye DING Shi LIU Ju college of pharmacy of Liaoning University Key Laboratory of New Drug Research and Development of Liaoning Province

The title compound 2-[2-(4-fluorobenzylidene)hydrazinyl]-4-(1-methyl-1 H-indol-3-yl)thieno[3,2-d] pyrimidine(8) was synthesized by the condensation of 4-fluorobenzaldehyde(7) with 2-hydrazinyl-4-(1-methyl-1 H-indol-3-yl)thieno[3,2-d]pyrimidine(6). This intermediate was prepared from methyl 3-aminothiophene-2-carboxylate(1) by the condensation with urea, chlorination with phosphorus oxychloride and then condensation with hydrazine hydrate. The crystal of 8 belongs to monoclinic system, space group P21/c with a = 14.0453(18), b = 17.436(2), c = 18.0982(17) ? and β = 122.969(7)°. In addition, 8 possesses marked inhibition against the proliferation of human colon cancer cell line HT-29(IC50 = 6.09 μM) and human gastric cancer cell line MKN45(IC50 = 3.04 μM), displaying promising anticancer activity.

关键词： thieno[3,2-d] pyrimidine synthesis X-ray diffraction antitumor activity

Förster resonance energy transfer reveals phillygenin and swertiamarin concurrently target AKT on different binding domains to increase the anti-inflammatory effect

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Chinese Chemical Letters 2024年第5期35卷 207-212页

作者： Xiaoyao Ma Jinling Zhang Ge Fang He Gao Jie Gao Li Fu Yuanyuan Hou Gang Bai State key laboratory of Medicinal Chemical Biology College of Pharmacy and Tianjin Key Laboratory of Molecular Drug ResearchNankai UniversityTianjin 300000China college of Life Health Dalian UniversityDalian 116622China Dalian Fusheng Pharmaceutical Co. Ltd.Dalian 116000China

The clinical benefit of combination therapy is significant,but it is not easy to define the mechanism of complexity and *** studies illustrate that phillygenin(Phi)binds in the allosteric inhibit pocket of protein kinase B(AKT),and swertiamarin(Swe)acts on the pleckstrin homology(PH)domain of ***,the combined synergistic effect of relieving the inflammatory response has yet to be *** on high sensitivity,specificity and fast-responsibility fluorescent sensors,the Förster resonance energy transfer(FRET)technique offers a route to provide clear insights into physiological and pathological *** the study,molecular docking,the fluorescent probes of Phi and Swe for FRET were designed and *** analysis shown that Swe and Phi concurrently acted on the PH domain and allosterically inhibited pocket of AKT,*** combination of Swe and Phi significantly increased the heat stability of AKT and decreased protease-induced *** lipopolysaccharides(LPS)-induced mice and cells,the combination arrested AKT activation,nuclear factor kappa-B(NF-κB)phosphorylation,and the expression of tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and *** conclusion,FRET revealed Phi and Swe concurrently targeted AKT on different domains and the combination of Phi and Swe enhanced the anti-inflammatory effect.

关键词： FRET Swertiamarin Phillygenin AKT Anti-inflammation Combinatorial drug

Supramolecular hydrogels of self-assembled zwitterionic-peptides

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Chinese Chemical Letters 2021年第11期32卷 3636-3640页

作者： Yiming Zhang Yinghao Ding Xinxin Li Debin Zheng Jie Gao Zhimou Yang State key laboratory of Medicinal Chemical Biology Key Laboratory of Bioactive MaterialsMinistry of Education and College of Life SciencesNankai UniversityTianjin 300071China college of Life Sciences Key Laboratory of Bioactive MaterialsMinistry of Educationand Collaboration Innovation Center of Chemical Science and Engineering(Tianjin)Nankai UniversityTianjin 300071China State key laboratory of Medicinal Chemical Biology College of PharmacyTianjin Key Laboratory of Molecular Drug ResearchNankai UniversityTianjin 300071China

Zwitterionic polymer materials have been extensively studied,but zwitterionic peptides supramolecular hydrogel materials are rarely *** this study,the preparation of two zwitterionic hydrogels using self-assembled peptides were *** hydrogels could be fabricated easily by changing the temperature or enzyme catalysis in a short *** the differences in structure and function of the zwitterion peptide hydrogels caused by the two preparation methods were also be *** found that the hydrogel prepared by enzyme induced self-assembly has better solubility and lower cytotoxicity than that prepared by the heating-cooling *** result showed the enzyme induced self-assembly way to form zwitterionic peptides supramolecular hydrogel materials could have further biomedical applications.

关键词： Self-assembly Hydrogel Zwitterionic-peptides Enzyme-instructed Biocompatibility

RHBDF1 promotes AP-1-activated endothelial-mesenchymal transition in tumor fibrotic stroma formation

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Signal Transduction and Targeted Therapy 2021年第8期6卷 2307-2310页

作者： Shan Gao Li-Song Zhang Lei Wang Nan-Nan Xiao Hui Long Yi-Lun Yin Yu-Meng Yang Zhen Xi Lu-Yuan Li Zhi-Song Zhang State key laboratory of Medicinal Chemical Biology College of PharmacyTianjin Key Laboratory of Molecular Drug ResearchNankai UniversityTianjinChina State key laboratory of Elemento-Organic Chemistry and Department of Chemical Biology National Pesticide Engineering Research Center(Tianjin)Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)College of ChemistryNankai UniversityTianjinChina

Dear Editor,There is a growing body of evidence that the human rhomboid family-1 gene(RHBDF1)plays an important role in the modulation of tumor inflammatory(Supplementary ***)and hypoxic microenvironment.

关键词： tumor transition hypoxic

New tirucallane-type triterpenoids from the resin of Boswellia carterii and their NO inhibitory activities

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Chinese Journal of Natural Medicines 2021年第9期19卷 686-692页

作者： LIU Fang-Shen ZHANG Ting-Ting XU Jun JING Qin-Xue GONG Chi DONG Bang-Jian LI Da-Hong LIU Xiao-Qiu LI Zhan-Lin YUAN Zhong HUA Hui-Ming key laboratory of Structure-Based drug Design&Discovery Ministry of EducationShenyang Pharmaceutical UniversityShenyang 110016China School of Traditional Chinese Materia Medica Shenyang Pharmaceutical UniversityShenyang 110016China State key laboratory of Medicinal Chemical Biology College of Pharmacyand Tianjin Key Laboratory of Molecular Drug ResearchNankai UniversityTianjin 300071China

Six new tirucallane-type triterpenoids(1-6),along with ten known triterpenoids,were isolated from methylene chloride extract of the resin of Boswellia carterii *** the application of the comprehensive spectroscopic data,the structures of the compounds were *** experimental electronic circular dichroism spectra were compared with those calculated,which allowed to assign the absolute *** 5 and 6 possesed a 2,3-seco tirucallane-type triterpenoid skeleton,which were first *** inhibitory activity against NO formation in LPS-activated BV-2 cells were *** 9 showed appreciable inhibitory effect,with an IC_(50)value of 7.58±0.87μmol·L^(-1).

关键词： Boswellia carterii Birdw seco-Tirucallane triterpenoids NO inhibition

Discovery of antitumor diterpenoids from Casearia graveolens targeting VEGFR-2 to inhibit angiogenesis

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Chinese Journal of Natural Medicines 2024年第9期22卷 842-853页

作者： WANG Sibei LIU Yuhui LIANG Yue XI Yaru ZHAI Yupeng LEE Dongho XU Jing GUO Yuanqiang State key laboratory of Medicinal Chemical Biology College of Pharmacyand Tianjin Key Laboratory of Molecular Drug ResearchNankai UniversityTianjin 300350China State key laboratory of Functions and Applications of Medicinal Plants Guizhou Medical UniversityGuiyang 550014China Department of Plant Biotechnology College of Life Sciences and BiotechnologyKorea UniversitySeoul 02841South Korea

Eight novel clerodane diterpenoids(1-8)were isolated from the twigs of Casearia *** structures were elucidated through comprehensive nuclear magnetic resonance(NMR),high-resolution electrospray ionization mass spectrometry(HRESI-MS),and electronic circular dichroism(ECD)*** addition to structural determination,surface plasmon resonance(SPR)assays were conducted to investigate molecular interactions,revealing that compound 8 exhibited high affinity for vascular endothelial growth factor receptor 2(VEGFR2),a key regulator of tumor *** in vivo experiments demonstrated that compound 8 effectively inhibited angiogenesis and displayed significant antitumor activity by suppressing tumor proliferation and metastasis in zebrafish xenograft *** findings suggest that compound 8 holds promise as an anticancer lead compound targeting VEGFR-2 to obstruct tumor angiogenesis.

关键词： Clerodane diterpenoids Casearia graveolens Surface plasmon resonance Vascular endothelial growth factor receptor 2 Antitumor activity Zebrafish

Design,synthesis and in vivo anti-hyperglycemic activity of gem-dimethyl-bearing C-glucosides as SGLT2 inhibitors

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Chinese Chemical Letters 2011年第10期22卷 1215-1218页

作者： Wen Jing Zhao Yong Heng Shi Gui Long Zhao Yu Li Wang Hua Shao Li Da Tang Jian Wu Wang School of Chemistry and Chemical Engineering Shandong University Jinan 250100 China tianjin key laboratory of molecular Design and drug Discovery Tianjin Institute of Pharmaceutical Research Tianjin 300193 China School of pharmacy Tianjin Medical University Tianjin 300070 China

A series of gem-dimethyl-bearing C-glucosides were designed and synthesized as SGLT2 inhibitors,with anhydrous aluminum chloride-mediated Friedel-Crafts alkylation to construct the gem-dimethyl functionality being the key *** in vivo anti-hyperglycemic activity was evaluated with mice oral glucose tolerance test（OGTT）,and all the synthesized compounds showed significant but less potent anti-hyperglycemic activity than the positive control dapagliflozin.

关键词： Synthesis C-Glucoside SGLT2 inhibitor Anti-hyperglycemic activity gem-Dimethyl

Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第2期13卷 118-125页

作者： Jian YI Meng MENG Zhong-qiu LIU Jin-fang ZHI Yuan-yang ZHANG Jing XU Ya-bin WANG Jin-ting LIU Ri-mo XI School of Chemistry and Chemical Engineering Shandong UniversityJinan 250100China college of pharmacy and tianjin key laboratory of molecular drug research Nankai UniversityTianjin 300071China Technical Institute of Physics and Chemistry Chinese Academy of SciencesBeijing 100080China

To detect gatifloxacin （GAT） residue in swine urine, an electrochemical immunoassay was established, An indirect competitive immunoassay was developed, in which the coating antigen is immobilized in an enzyme-linked immunosorbent assay （ELISA） plate and GAT residue from the sample competes with the limited binding sites in added anti-GAT antibody. Horseradish peroxidase （HRP） conjugated to goat anti-rabbit IgG was used as the enzymatic label. A carbon fiber working electrode was constructed and current signals were detected by using hydrogen peroxide as a substrate and hydroquinone as an electrochemical mediator. The electrochemical immunoassay was evaluated by analysis of GAT in buffer or swine urine and an average value of half inhibition concentration （IC50） of 8.9 ng/ml was obtained. Excellent specificity of the antibody was achieved with little cross-reaction with Iomefloxacin （3.0%）, ciprofloxacin （3.0%）, and ofloxacin （1.9%） among commonly used （fluoro）quinolones. In conclusion, the im- munoassay system developed in this research can be used as a rapid, powerful and on-site analytical tool to detect GAT residue in foods and food products.

关键词： Gatifloxacin drug residue Antibody Carbon fiber electrode Electrochemical immunoassay