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Mutation in the Agrobacterium his I gene enhances transient expression in pepper

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Horticultural Plant Journal 2024年第3期10卷 809-822页

作者： Dan Liu Shengnan Zhao Jubin Wang Xi Zhang Yingtian Deng Feng Li National Key Laboratory for Germplasm Innovation&Utilization of Horticultural crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China

Agrobacterium-mediated plant transformation is widely used in plant genetic ***,its efficiency is limited by plant immunity against *** pepper(Capsicum annuum L.)is an important vegetable that is recalcitrant to Agrobacterium-mediated *** this work,Agrobacterium was found to induce a strong immune response in pepper,which might be the reason for T-DNA being difficult to express in *** Agrobacterium mutant screen was conducted and a point mutation in the hisI gene was identified due to a weak immune response and enhanced transient expression mediated by this Agrobacterium mutant in pepper *** genetic analysis revealed that histidine biosynthesis deficiency caused by mutations in many genes of this pathway led to reduced pepper cell death,presumably due to reduced bacterial ***,mutation analysis of threonine and tryptophan biosynthesis genes showed that the biosynthesis of different amino acids may play different roles in Agrobacterium growth and stimulating the pepper immune *** possible application of Agrobacterium amino acid biosynthesis mutations in plant biology was discussed.

关键词： Capsicum annuum L. Agrobacterium Transient transformation efficiency Mutation Amino acid Immune response

Identification of the lysine and histidine transporter family in Camellia sinensis and the characterizations in nitrogen utilization

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Horticultural Plant Journal 2024年第1期10卷 273-287页

作者： Wei Huang Danni Ma Fawad Zaman Xulei Hao Li Xia E Zhang Pu Wang Mingle Wang Fei Guo Yu Wang Dejiang Ni Hua Zhao National Key Laboratory for Germplasm Innovation&Utilization of Horticultural crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China

In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino *** tea plant(Camellia sinensis)is a perennial evergreen with a relatively high level of amino ***,systematic identification and molecular characterization of the LHT gene family has rarely been reported in tea *** this study,22 CsLHTs were identified from the‘Shuchazao’genome and classified into two *** modeled three-dimensional structure and the conserved domains presented a high similarity among the LHTs ***,it was predicted that a few genes were conserved through the analysis of the physiochemical characters,structures and cis-elements in *** expression patterns in tea plants revealed that CsLHT7 was mainly expressed in the roots,and CsLHT4 and CsLHT11 exhibited relatively high expression in both the roots and ***,the expression of all three genes could be induced by organic ***,heterogeneous expression of CsLHT4,CsLHT7 and CsLHT11 in Arabidopsis thaliana decreased the aerial parts biomass compared with that in WT plants while significantly increased the rosette biomass only for CsLHT11transgenic plants versus WT ***,our results provide fundamental information about CsLHTs and potential genes in N utilization for further analysis in tea plants.

关键词： Camellia sinensis Nitrogen Lysine and histidine transporter(LHT)family

Surface engineering of carbon dots for highly sensitive α-glucosidase assay and inhibition evaluation

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Chinese Chemical Letters 2024年第3期35卷 193-197页

作者： Meijuan Liang Gege Song Yeqing Wan Yingying Chen Fuan Wang Xiaoqing Liu college of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072China Oil crops Research Institute Chinese Academy of Agricultural SciencesWuhan 430062China

Monitoringα-glucosidase(α-Glu)activity is of great significance for the early diagnosis of typeⅡ*** the blue fluorescent carbon dots(CDs)were integrated with two different recognizing molecules,β-cyclodextrin and phenylboronic acid,for assembling a multifunctional CDs(mCDs)nanoplatform for sensitively analyzingα-Glu and its *** hydrolyzed product of 4-nitrophenyl-α-D-glucopyranoside(α-Glu substrate),p-nitrophenol,could efficiently quench the fluorescence of mCDs due to its cooperative molecular recognition withβ-cyclodextrin and phenylboronic *** mCDs could be utilized for the detection ofα-Glu activity with the limit of detection of 0.030 U/***,the presentα-Glu detection platform revealed a high selectivity,and other natural enzymes showed scarcely any effect on the present mCDs *** proposed method could be facilely used to screenα-Glu inhibitors with satisfying *** rational mCDs is expected to supplement more comprehensive biosensing platforms for highly sensitive and specific recognition of disease-relevant biomarkers with clinical importance.

关键词： α-Glucosidase Carbon dots Fluorescent sensor Inhibitor β-Cyclodextrin

GhWRKY75 positively regulates GhPR6-5b via binding to a W-box TTGAC(C/T)to orchestrate cotton resistance to Verticillium dahliae

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Journal of Integrative Agriculture 2024年第10期23卷 3343-3357页

作者： Qichao Chai Meina Zheng Yanli Li Mingwei Gao Yongcui Wang Xiuli Wang Chao Zhang Hui Jiang Ying Chen Jiabao Wang Junsheng Zhao Institute of Industrial crops Shandong Academy of Agricultural SciencesJinan 250100China college of Life Sciences Shandong Normal UniversityJinan 250014China

Verticillium dahliae is an important fungal pathogen affecting cotton yield and ***,the mining of ***-resistance genes is urgently *** and protease inhibitors play crucial roles in plant defense ***,the functions and regulatory mechanisms of the protease inhibitor PR6 gene family remain largely *** study provides a comprehensive analysis of the PR6 gene family in the cotton *** performed genome-wide identification and functional characterization of the cotton GhPR6 gene family,which belongs to the potato protease inhibitor I family of ***-nine PR6s were identified in Gossypium arboreum,***,***,and ***,and they were clustered into four *** on the analysis of pathogen-induced and Ghlmm transcriptome data,Gh PR6-5b was identified as the key gene for *** ***-induced gene silencing experiments revealed that cotton was more sensitive to *** V991after PR6-5b *** present study established that GhWRKY75 plays an important role in resistance to Verticillium wilt in cotton by positively regulating GhPR6-5b expression by directly binding to the W-box TTGAC(T/C).Our findings established that GhWRKY75 is a potential candidate for improving cotton resistance to ***,and provide primary information for further investigations and the development of specific strategies to bolster the defense mechanisms of cotton against ***.

关键词： cotton proteinase inhibitors WRKY transcription factor Verticillium wilt

Screening and identification of salt tolerance soybean varieties and germplasms

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Oil Crop Science 2024年第3期9卷 204-210页

作者： Limiao Chen Lihua Peng Wenqi Ouyang Haowen Yao Yuxin Ye Zhihui Shan Dong Cao Shuilian Chen Zhonglu Yang Yi Huang Bei Han Aihua Sha Xinan Zhou Haifeng Chen Key Laboratory of Biology and Genetic Improvement of Oil crops Ministry of Agriculture and Rural AffairsOil Crops Research Institute of Chinese Academy of Agricultural SciencesWuhan430062China college of Agriculture Yangtze UniversityJingzhou434000China

Soil salinization is a globally prevalent abiotic environmental *** imbalance of ions caused by high concentrations of sodium chloride results in a 40%reduction in soybean ***,as an important crop for soil quality improvement,necessitates the identification of salt-tolerant varieties and germplasms to effectively utilize and enhance saline-alkali *** this study,we assessed the salt tolerance of 435 soybean varieties and germplasms during the seedling *** them,Qihuang34,You2104,Hongzhudou,Pamanheidou,and Osage exhibited grade 1 salt tolerance rates surpassing other tested ***,Hongzhudou and Qihuang34 demonstrated higher salt tolerance during germination and emergence stages based on their elevated rates of emergence,salt tolerance index,chlorophyll content,and shoot fresh *** findings provide valuable resources for molecular breeding efforts aimed at developing salt-tolerant soybean varieties suitable for cultivation in saline-alkali soils.

关键词： Soybean Varieties Germplasms Salt tolerance Seedling stage Germination and emergence stage

Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean(Vigna radiata L.)

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Journal of Integrative Agriculture 2024年第8期23卷 2571-2588页

作者： Qian Wang Huimin Cao Jingcheng Wang Zirong Gu Qiuyun Lin Zeyan Zhang Xueying Zhao Wei Gao Huijun Zhu Hubin Yan Jianjun Yan Qingting Hao Yaowen Zhang college of Agronomy Shanxi Agricultural UniversityTaiyuan 030031China 2 Tropical crops Genetic Resources Institute Chinese Academy of Tropical Agricultural SciencesHaikou 571101China

Seed coat color affects the appearance and commodity quality of mung beans(Vigna radiata L.).The substances that affect mung bean seed coat color are mainly flavonoids,which have important medicinal *** the seed coat color gene in mung beans would facilitate the development of new varieties and improve their *** this study,an F2 mapping population consisting of 546 plants was constructed using Jilv9(black seed coat)and BIS9805(green seed coat).Using bulk segregated analysis(BSA)sequencing and kompetitive allele-specific PCR(KASP)markers,the candidate region related to seed coat color was finally narrowed to 0.66 Mb on chromosome(Chr.)4 and included eight candidate *** transcriptome and metabolome analyses showed that three of the eight candidate genes(LOC106758748,LOC106758747,and LOC106759075)were differentially expressed,which may have caused the differences in flavonoid metabolite content between Jilv9 and *** findings can provide a research basis for cloning the genes related to seed coat color and accelerate molecular markerassisted selection breeding in mung beans.

关键词： mung bean seed coat color bulk segregated analysis sequencing transcriptome metabolism flavonoids

Spherical Polyelectrolyte Brush Nanoreactor:Preparation of Hollow TiO_(2) Nanospheres and Characterization by Small Angle X-Ray Scattering

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Chinese Journal of Polymer Science 2024年第9期42卷 1393-1400,I0009页

作者： Yu-Hua Zhang Zi-Yu Zhang Xin Liu En-Guang Ma Jiang-Tao Guo Li Li Xu-Hong Guo State Key Laboratory of Chemical Engineering East China University of Science and TechnologyShanghai200237China Shandong Provincial Key Laboratory of Monocrystalline Silicon Semiconductor Materials and Technology College of Chemistry and Chemical EngineeringDezhou UniversityDezhou253023China Institute of Bast Fiber crops&Center of Southern Economic crops Chinese Academy of Agricultural SciencesChangsha410205China

Titanium dioxide(TiO_(2))hollow nanoparticles present significant potential for photocatalytic applications while their straightforward preparation with precise structure control is still *** work reports the approach to preparing tunable hollow TiO_(2) nanospheres by utilization of spherical polyelectrolyte brushes(SPB)as nanoreactors and *** the preparation,the evolution of the structure was characterized by small angle X-ray scattering(SAXS),and in combination with dynamic light scattering and transmission electron *** formation of TiO_(2) shell within the brush(SPB@TiO_(2))is confirmed by the significant increase of the electron density,and its internal structure has been analyzed by fitting SAXS data,which can be influenced by Titanium precursors and ammonia *** calcining SPB@TiO_(2) in a muffle furnace,hollow TiO_(2) nanospheres are produced,and their transition to the anatase crystal form is triggered,as confirmed by X-ray diffraction *** the advantages of their hollow structure,these TiO_(2) nanospheres exhibit exceptional catalytic degradation efficiency of methylene blue(MB),tetracycline(TC),and 2,4-dichlorophenoxyacetic acid(2,4-D),and also demonstrate excellent recyclability.

关键词： Spherical polyelectrolyte brush Nanoreactor Hollow titanium dioxide X-ray scattering

The cytosolic isoform of triosephosphate isomerase,ZmTPI4,is required for kernel development and starch synthesis in maize(Zea mays L.)

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The Crop Journal 2024年第2期12卷 401-410页

作者： Wenyu Li Han Wang Qiuyue Xu Long Zhang Yan Wang Yongbiao Yu Xiangkun Guo Zhiwei Zhang Yongbin Dong Yuling Li State Key Laboratory of Wheat and Maize Crop Science Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityLongzi Lake CampusZhengzhou 450046HenanChina

Triosephosphate isomerase(TPI)is an enzyme that functions in plant energy production,accumulation,and *** understand its function in maize,we characterized a maize TPI mutant,*** comparison to the wild type,zmtpi4 mutants showed altered ear development,reduced kernel weight and starch content,modified starch granule morphology,and altered amylose and amylopectin ***,ATP,and pyruvate contents were reduced,indicating ZmTPI4 was involved in *** subcellular localization confirmed ZmTPI4 as a cytosolic rather than a plastid isoform of TPI,the zmtpi4 mutant showed reduced leaf size and chlorophyll *** of ZmTPI4 in Arabidopsis led to enlarged leaves and increased seed weight,suggesting a positive regulatory role of ZmTPI4 in kernel weight and starch *** conclude that ZmTPI4 functions in maize kernel development,starch synthesis,glycolysis,and photosynthesis.

关键词： Maize Kernel Starch Weight Photosynthesis

Functional prediction of tomato PLATZ family members and functional verification of Sl PLATZ17

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Journal of Integrative Agriculture 2024年第1期23卷 141-154页

作者： Min Xu Zhao Gao Dalong Li Chen Zhang Yuqi Zhang Qian He Yingbin Qi He Zhang Jingbin Jiang Xiangyang Xu Tingting Zhao college of Horticulture and Landscape Architecture Northeast Agricultural UniversityHarbin 150030China Key Laboratory of Biology and Genetic Improvement of Horticultural crops(Northeast Region) Ministry of Agriculture and Rural AffairsHarbin 150030China

PLATZ is a novel zinc finger DNA-binding protein that plays an important role in regulating plant growth and development and resisting abiotic ***,there has been very little research on the function of this family gene in tomatoes,which limits its application in germplasm resource ***,the PLATZ gene family was identified and analyzed in tomato,and its roles were predicted and verified to provide a basis for in-depth research on SlPLATZ gene *** this study,the PLATZ family members of tomato were identified in the whole genome,and 19 SlPLATZ genes were *** prediction was conducted based on gene and promoter structure analysis and RNA-seq-based expression pattern *** genes that responded significantly under different abiotic stresses or were significantly differentially expressed among multiple tissues were screened as functional gene ***17 was selected for functional verification by experiment-based *** results showed that the downregulation of SlPLATZ17 gene expression reduced the drought and salt tolerance of tomato *** plants overexpressing SlPLATZ17 had larger flower sizes and long,thin petals,adjacent petals were not connected at the base,and the stamen circumference was *** study contributes to understanding the functions of the SlPLATZ family in tomato and provides a reference for functional gene screening.

关键词： tomato PLATZ family abiotic stresses plant development