Mutation in the Agrobacterium his I gene enhances transient expression in pepper

作     者：Dan Liu Shengnan Zhao Jubin Wang Xi Zhang Yingtian Deng Feng Li Dan Liu;Shengnan Zhao;Jubin Wang;Xi Zhang;Yingtian Deng;Feng Li

作者机构：National Key Laboratory for Germplasm Innovation&Utilization of Horticultural CropsCollege of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2024年第10卷第3期

页      面：809-822页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：supported by the National Key Research and Development Program of China(Grant No.2018YFD1000800) National Natural Science Foundation of China(Grant No.32172600) 

主　　题：Capsicum annuum L. Agrobacterium Transient transformation efficiency Mutation Amino acid Immune response 

摘      要：Agrobacterium-mediated plant transformation is widely used in plant genetic ***,its efficiency is limited by plant immunity against *** pepper(Capsicum annuum L.)is an important vegetable that is recalcitrant to Agrobacterium-mediated *** this work,Agrobacterium was found to induce a strong immune response in pepper,which might be the reason for T-DNA being difficult to express in *** Agrobacterium mutant screen was conducted and a point mutation in the hisI gene was identified due to a weak immune response and enhanced transient expression mediated by this Agrobacterium mutant in pepper *** genetic analysis revealed that histidine biosynthesis deficiency caused by mutations in many genes of this pathway led to reduced pepper cell death,presumably due to reduced bacterial ***,mutation analysis of threonine and tryptophan biosynthesis genes showed that the biosynthesis of different amino acids may play different roles in Agrobacterium growth and stimulating the pepper immune *** possible application of Agrobacterium amino acid biosynthesis mutations in plant biology was discussed.