Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean(Vigna radiata L.)

作     者：Qian Wang Huimin Cao Jingcheng Wang Zirong Gu Qiuyun Lin Zeyan Zhang Xueying Zhao Wei Gao Huijun Zhu Hubin Yan Jianjun Yan Qingting Hao Yaowen Zhang Qian Wang;Huimin Cao;Jingcheng Wang;Zirong Gu;Qiuyun Lin;Zeyan Zhang;Xueying Zhao;Wei Gao;Huijun Zhu;Hubin Yan;Jianjun Yan;Qingting Hao;Yaowen Zhang

作者机构：College of AgronomyShanxi Agricultural UniversityTaiyuan 030031China 2 Tropical Crops Genetic Resources InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2024年第23卷第8期

页      面：2571-2588页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(32301928) the Basic Research Program of Shanxi Province,China(20210302124504) the China Agriculture Research System of MOF and MARA-Food Legumes(CARS08-G10) the National Laboratory Project of Coarse Grain Germplasm Resources Innovation and Molecular Breeding,China(K462202040-01) the Ph D of Shanxi Agricultural University Scientific Research Start-up Project,China(2021BQ43) the Scientific Research Project of Shanxi Agricultural University,China(YZGC098) 

主　　题：mung bean seed coat color bulk segregated analysis sequencing transcriptome metabolism flavonoids 

摘      要：Seed coat color affects the appearance and commodity quality of mung beans(Vigna radiata L.).The substances that affect mung bean seed coat color are mainly flavonoids,which have important medicinal *** the seed coat color gene in mung beans would facilitate the development of new varieties and improve their *** this study,an F2 mapping population consisting of 546 plants was constructed using Jilv9(black seed coat)and BIS9805(green seed coat).Using bulk segregated analysis(BSA)sequencing and kompetitive allele-specific PCR(KASP)markers,the candidate region related to seed coat color was finally narrowed to 0.66 Mb on chromosome(Chr.)4 and included eight candidate *** transcriptome and metabolome analyses showed that three of the eight candidate genes(LOC106758748,LOC106758747,and LOC106759075)were differentially expressed,which may have caused the differences in flavonoid metabolite content between Jilv9 and *** findings can provide a research basis for cloning the genes related to seed coat color and accelerate molecular markerassisted selection breeding in mung beans.