检索结果-南通市图书馆

Efficient degradation of aqueous dichloromethane by an enhanced microbial electrolysis cell:Degradation kinetics,microbial community and metabolic mechanisms

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第5期139卷 150-159页

作者： Meng Wu Di Zhao Bing Gu ZiruWang Jun Hu Zhiliang Yu Jianming Yu college of Environment College of Biotechnology and BioengineeringCollaborative Innovation Center of Yangtze River Delta Region Green PharmaceuticalsZhejiang University of TechnologyHangzhou 310014China Shentuo Environment(Hangzhou)Co.Ltd. Hangzhou 311121China Zhejiang Tianyi Environmental Co.Ltd. Hangzhou 310000China

Dichloromethane(DCM)has been listed as a toxic and harmful water pollutant,and its re moval needs attention.Microbial electrolysis cells(MECs)are viewed as a promising alterna tive for pollutant removal,which can be strengthened from two aspects:microbial inocula tion and acclimation.In this study,the MEC for DCM degradation was inoculated with the ac tive sludge enhanced by Methylobacterium rhodesianum H13(strain H13)and then acclimated in the form of a microbial fuel cell(MFC).Both the introduction of strain H13 and the initi ation in MFC form significantly promoted DCM degradation.The degradation kinetics were fitted by the Haldane model,with V_(max),K_(h),K_(i)and v_(max)values of 103.2 mg/L/hr,97.8 mg/L268.3 mg/L and 44.7 mg/L/hr/cm^(2),respectively.The cyclic voltammogram implies that DCM redox reactions became easier with the setup of MEC,and the electrochemical impedance spectrogram shows that the acclimated and enriched microbes reduced the charge transfe resistance from the electrode to the electrolyte.In the biofilm,the dominant genera shifted from Geobacter to Hyphomicrobium in acclimation stages.Moreover,Methylobacterium played an increasingly important role.DCM metabolism mainly occurred through the hydrolytic glutathione S-transferase pathway,given that the gene dcmA was identified rather than the dhlA and P450/MO.The exogenous electrons facilitated the reduction of GSSG,directly o indirectly accelerating the GSH-catalyzed dehalogenation.This study provides support fo the construction of an efficient and stable MEC for DCM removal in water environment.

关键词： Dichloromethane Microbial electrolysis cells Metabolic pathways Electron transfer Degradation kinetics

Effect of hormonal status and metabolic changes of restricted ewes during late pregnancy on their fetal growth and development

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2007年第6期50卷 766-772页

作者： GAO Feng1, HOU XianZhi1 & LIU YingChun2 1 college of Animal Science and Animal Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China 2 college of biotechnology and bioengineering, Inner Mongolia Agricultural University, Huhhot 010018, China college of Animal Science and Animal Medicine Inner Mongolia Agricultural University Huhhot China college of biotechnology and bioengineering Inner Mongolia Agricultural University Huhhot China

This study investigated the effects of hormonal status and metabolic changes of restricted ewes during late pregnancy on the ovine fetus growth and development. One hundred Mongolian ewes, synchro- nized for oestrus and mated, were divided into three groups and offered 0.175 MJME·kgw-0.75·d-1 (Re- stricted Group 1, RG1), 0.33 MJME·kgw-0.75·d-1 (Restricted Group 2, RG2) and ad libitum access to feed (Control Group, CG) during their late pregnancy respectively. The results suggested that with the sup- ply of exogenous energy decreasing during late pregnancy, maternal body weight and net body weight loss in RG2 and RG1 were lower than those of CG (P0.05), but the GH concentrations in RG2 and RG1 were enhanced and there was significant difference between RG1 and CG on d 120 of gesta- tion (P0.05). However, the NEFA and FAA concentrations of ewes in RG1 were reduced from d 90 to d 120 of gestation, then enhanced from d 120 to d 140 of gestation. During the late pregnancy, with the supply of nutrition decreasing, the negatively physiological and biochemical maternal reactions to restriction became worse, which sig- nificantly reduced the average lamb birth weight and daily growth rate of fetus in RG2 (P<0.05) and RG1 (P<0.01).

关键词： late pregnancy, restricted ewes, fetus, growth and development

RNA barcode segments for SARS-CoV-2 identification from HCoVs and SARSr-CoV-2 lineages

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2024年第1期39卷 156-168页

作者： Changqiao You Shuai Jiang Yunyun Ding Shunxing Ye Xiaoxiao Zou Hongming Zhang Zeqi Li Fenglin Chen Yongliang Li Xingyi Ge Xinhong Guo college of Biology Hunan UniversityChangsha410082China college of Bioscience and biotechnology Hunan Agricultural UniversityChangsha410128China

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the pathogen responsible for coronavirus disease 2019(COVID-19),continues to evolve,giving rise to more variants and global reinfections.Previous research has demonstrated that barcode segments can effectively and cost-efficiently identify specific species within closely related populations.In this study,we designed and tested RNA barcode segments based on genetic evolutionary relationships to facilitate the efficient and accurate identification of SARS-CoV-2 from extensive virus samples,including human coronaviruses(HCoVs)and SARSr-CoV-2 lineages.Nucleotide sequences sourced from NCBI and GISAID were meticulously selected and curated to construct training sets,encompassing 1733 complete genome sequences of HCoVs and SARSr-CoV-2 lineages.Through genetic-level species testing,we validated the accuracy and reliability of the barcode segments for identifying SARS-CoV-2.Subsequently,75 main and subordinate species-specific barcode segments for SARS-CoV-2,located in ORF1ab,S,E,ORF7a,and N coding sequences,were intercepted and screened based on single-nucleotide polymorphism sites and weighted scores.Post-testing,these segments exhibited high recall rates(nearly 100%),specificity(almost 30%at the nucleotide level),and precision(100%)performance on identification.They were eventually visualized using one and two-dimensional combined barcodes and deposited in an online database(http://virusbarcodedatabase.top/).The successful integration of barcoding technology in SARS-CoV-2 identification provides valuable insights for future studies involving complete genome sequence polymorphism analysis.Moreover,this cost-effective and efficient identification approach also provides valuable reference for future research endeavors related to virus surveillance.

关键词： RNA barcode segments SARS-CoV-2 variants and related lineages HCoVs Genetic tests Complete genome sequences

Selenium nanoparticles biosynthesized by Eurotium cristatum with antimicrobial activity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年

作者： Nanyang Li Yanru Yang Jianrui Qi Jiahui Li Yifan Cheng Zhao Li Tianli Yue Yahong Yuan college of Food Science and Engineering Northwest A&F University college of Food Science and Technology Northwest University Xi'an college of bioengineering and biotechnology Tianshui Normal University

Selenium nanoparticles (SeNPs) are increasingly recognized for their exceptional antibacterial properties. This study aimed to develop a green， safe， and efficient method for the biosynthesis of SeNPs using the fungus Eurotium cristatum (E. cristatum)， a novel approach in SeNP synthesis. The process yielded (36.4 ± 4.22) mg of SeNPs per 1.2 mmol/L sodium selenite supplementation. These SeNPs exhibited an average diameter of 231.7 nm and a negative charge， and they remained stable when stored at 4℃. UV-vis revealed a maximum absorption peak at 212 nm， suggesting effective nanoparticle formation. FT-IR indicated that proteins and carbohydrates in the mycelium contributed to the SeNP synthesis. Concentrations of SeNPs below 50 μg Se/mL did not exhibit cytotoxic effects on the growth and proliferation of human hepatocyte L-02 cells. The minimum inhibitory concentration of SeNPs was found to be 2 mg/mL against both Methicillin-resistant Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative). The SeNPs compromised the cellular integrity of test strains， causing leakage of intracellular contents and disruption of the oxidative stress system， leading to irreversible damage. Our results demonstrate the potential of SeNPs biosynthesized by E. cristatum to act as effective antibacterial agents， signifying a novel and promising approach to developing natural antimicrobial solutions.

关键词：

A pipeline to characterize p53 effectors by integrative cistrome and transcriptome analysis in a genetically-defined organoid model

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第2期11卷 512-515页

作者： June-Ha Shin Hwa-Ryeon Kim Jae-Seok Roe Department of Biochemistry College of Life Science and BiotechnologyYonsei UniversitySeoul 120-749Republic of Korea

Tumor-protein 53(p53)is a transcription factor(TF)encoded by TP53(Trp53 in mice)and is a master regulator of tumor-suppressive programs'.Importantly,TP53 is the most frequently mutated gene in human cancer,and its loss of function contributes to tumor development,exemplified by variable p53 mutations detected in 50%-75%of pancreatic cancers.2 From these perceptions,a number of targets transcribed by p53 that confer tumor suppression have been identified.Nevertheless,our understanding of how p53 prevents normal cells from initiating tumorigenesis is still incomplete.To complement this limitation,herein,we established a new approach to identify underlying effectors transcribed by p53 in normal physiology.

关键词： incomplete initiating herein

Harness High-Temperature Thermal Energy via Elastic Thermoelectric Aerogels

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano-Micro Letters 2024年第8期16卷 196-210页

作者： Hongxiong Li Zhaofu Ding Quan Zhou Jun Chen Zhuoxin Liu Chunyu Du Lirong Liang Guangming Chen college of Materials Science and Engineering Shenzhen UniversityShenzhen 518055People’s Republic of China Department of bioengineering University of CaliforniaLos AngelesCA 90095USA

Despite notable progress in thermoelectric(TE)materials and devices,developing TE aerogels with high-temperature resistance,superior TE performance and excellent elasticity to enable self-powered high-temperature monitoring/warning in industrial and wearable applications remains a great challenge.Herein,a highly elastic,flame-retardant and high-temperature-resistant TE aerogel,made of poly(3,4-ethylene dioxythiophene):poly(styrenesulfonate)/single-walled carbon nanotube(PEDOT:PSS/SWCNT)composites,has been fabricated,displaying attractive compression-induced power factor enhancement.The as-fabricated sensors with the aerogel can achieve accurately pressure stimuli detection and wide temperature range monitoring.Subsequently,a flexible TE generator is assembled,consisting of 25 aerogels connected in series,capable of delivering a maximum output power of 400μW when subjected to a temperature difference of 300 K.This demonstrates its outstanding high-temperature heat harvesting capability and promising application prospects for real-time temperature monitoring on industrial high-temperature pipelines.Moreover,the designed self-powered wearable sensing glove can realize precise wide-range temperature detection,high-temperature warning and accurate recognition of human hand gestures.The aerogel-based intelligent wearable sensing system developed for firefighters demonstrates the desired self-powered and highly sensitive high-temperature fire warning capability.Benefitting from these desirable properties,the elastic and high-temperature-resistant aerogels present various promising applications including self-powered high-temperature monitoring,industrial overheat warning,waste heat energy recycling and even wearable healthcare.

关键词： Thermoelectrics Aerogel Self-powered High-temperature monitoring High-temperature warning

The two sides of chromosomal instability:drivers and brakes in cancer

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2024年第4期9卷 1595-1624页

作者： Rendy Hosea Sharon Hillary Sumera Naqvi Shourong Wu Vivi Kasim Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing 400045China The 111 Project Laboratory of Biomechanics and Tissue Repair College of BioengineeringChongqing UniversityChongqing 400044China Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment Chongqing University Cancer HospitalChongqing UniversityChongqing 400030China

Chromosomal instability(CIN)is a hallmark of cancer and is associated with tumor cell malignancy.CIN triggers a chain reaction in cells leading to chromosomal abnormalities,including deviations from the normal chromosome number or structural changes in chromosomes.CIN arises from errors in DNA replication and chromosome segregation during cell division,leading to the formation of cells with abnormal number and/or structure of chromosomes.Errors in DNA replication result from abnormal replication licensing as well as replication stress,such as double-strand breaks and stalled replication forks;meanwhile,errors in chromosome segregation stem from defects in chromosome segregation machinery,including centrosome amplification,erroneous microtubule-kinetochore attachments,spindle assembly checkpoint,or defective sister chromatids cohesion.In normal cells,CiN is deleterious and is associated with DNA damage,proteotoxic stress,metabolic alteration,cell cycle arrest,and senescence.Paradoxically,despite these negative consequences,CiN is one of the hallmarks of cancer found in over 90%of solid tumors and in blood cancers.Furthermore,CiN could endow tumors with enhanced adaptation capabilities due to increased intratumor heterogeneity,thereby facilitating adaptive resistance to therapies;however,excessive CiN could induce tumor cells death,leading to the"just-right"model for CIN in tumors.Elucidating the complex nature of CIN is crucial for understanding the dynamics of tumorigenesis and for developing effective anti-tumor treatments.This review provides an overview of causes and consequences of CIN,as well as the paradox of CIN,a phenomenon that continues to perplex researchers.Finally,this review explores the potential of CIN-based anti-tumor therapy.

关键词： chromosomal consequences thereby

Mechanism of action, benefits, and research gap in fermented soybean meal utilization as a high-quality protein source for livestock and poultry

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Nutrition 2024年第1期16卷 130-146页

作者： Modinat T.Lambo Haokai Ma Haosheng Zhang Peng Song Hongxiang Mao Guowen Cui Baisheng Dai Yang Li Yonggen Zhang college of Animal Science and Technology Northeast Agricultural University Wilmar (Shanghai) biotechnology Research and Development Center Co. Ltd college of Electrical Engineering and Information Northeast Agricultural University

Animal nutritionists have incessantly worked towards providing livestock with high-quality plant protein feed resources.Soybean meal (SBM) has been an essential and predominantly adopted vegetable protein source in livestock feeding for a long time;however,several SBM antinutrients could potentially impair the animal's performance and growth,limiting its use.Several processing methods have been employed to remove SBM antinutrients,including fermentation with fungal or bacterial microorganisms.According to the literature,fermentation,a traditional food processing method,could improve SBM's nutritional and functional properties,making it more suitable and beneficial to livestock.The current interest in health-promoting functional feed,which can enhance the growth of animals,improve their immune system,and promote physiological benefits more than conventional feed,coupled with the ban on the use of antimicrobial growth promoters,has caused a renewed interest in the use of fermented SBM (FSBM) in livestock diets.This review details the mechanism of SBM fermentation and its impacts on animal health and discusses the recent trend in the application and emerging advantages to livestock while shedding light on the research gap that needs to be critically addressed in future studies.FSBM appears to be a multifunctional high-quality plant protein source for animals.Besides removing soybean antinutrients,beneficial bioactive peptides and digestive enzymes are produced during fermentation,providing probiotics,antioxidants,and immunomodulatory effects.Critical aspects regarding FSBM feeding to animals remain uncharted,such as the duration of fermentation,the influence of feeding on digestive tissue development,choice of microbial strain,and possible environmental impact.

关键词： Soybean meal Protein feed Fermentation Microorganism Livestock

Emodin suppresses alkali burn-induced corneal inflammation and neovascularization by the vascular endothelial growth factor receptor 2 signaling pathway

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medicine 2024年第2期44卷 268-276页

作者： ZHENG Xueying GUO Liang LAI Siyi LI Fengyue LIANG Mingli LIU Wanting MENG Chun LIU Guanghui Department of bioengineering College of Biological Science and BiotechnologyFuzhou UniversityFuzhou 350104China Eye Institute of Integrated Chinese and Western Medicine Fujian University of Traditional Chinese MedicineFuzhou 350004China Department of Ophthalmology Affiliated People's Hospital(Fujian Provincial People's Hospital)Fujian University of Traditional Chinese MedicineFuzhou 350004China

OBJECTIVE:To investigate the effects of emodin on alkali burn-induced corneal inflammation and neovascularization.METHODS:The ability of emodin to target vascular endothelial growth factor receptor 2(VEGFR2)was predicted by molecular docking.The effects of emodin on the invasion,migration,and proliferation of human umbilical vein endothelial cells(HUVEC)were determined by cell counting kit-8,Transwell,and tube formation assays.Analysis of apoptosis was performed by flow cytometry.CD31 levels were examined by immunofluorescence.The abundance and phosphorylation state of VEGFR2,protein kinase B(Akt),signal transducer and activator of transcription 3(STAT3),and P38 were examined by immunoblot analysis.Corneal alkali burn was performed on 40 mice.Animals were divided randomly into two groups,and the alkali-burned eyes were then treated with drops of either 10μM emodin or phosphate buffered saline(PBS)four times a day.Slitlamp microscopy was used to evaluate inflammation and corneal neovascularization(CNV)in all eyes on Days 0,7,10,and 14.The mice were killed humanely 14 d after the alkali burn,and their corneas were removed and preserved at-80℃ until histological study or protein extraction.RESULTS:Molecular docking confirmed that emodin was able to target VEGFR2.The findings revealed that emodin decreased the invasion,migration,angiogenesis,and proliferation of HUVEC in a dose-dependent manner.In mice,emodin suppressed corneal inflammatory cell infiltration and inhibited the development of corneal neovascularization induced by alkali burn.Compared to those of the PBS-treated group,lower VEGFR2 expression and CD31 levels were found in the emodintreated group.Emodin dramatically decreased the expression of VEGFR2,p-VEGFR2,p-Akt,p-STAT3,and p-P38 in VEGF-treated HUVEC.CONCLUSION:This study provides a new avenue for evaluating the molecular mechanisms underlying corneal inflammation and neovascularization.Emodin might be a promising new therapeutic option for corneal alkali burns.

关键词： alkali burn emodin corneal inflammation corneal neovascularisation vascular endothelial growth factor receptor-2 signal transduction