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Fine mapping and identification of CaTTG1,a candidate gene that regulates the hypocotyl anthocyanin accumulation in Capsicum annuum

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Horticultural Plant Journal 2025年第1期11卷 264-274页

作者： Jin Wang Yao Dai Luzhao Pan Ying Chen Li Dai Yanqing Ma Xiaoxun Zhou Wu Miao Muhammad Rizwan Hamid Xuexiao Zou Feng Liu Cheng Xiong engineering Research Center for Germplasm Innovation and New Varieties Breeding of Horticultural Crops Key Laboratory for Vegetable Biology of Hunan ProvinceCollege of HorticultureHunan Agricultural UniversityChangshaHunan 410000China Hunan Xiangyan seed Industry Co. LTDChangshaHunan 410000China

Pepper(Capsicum annuum L.)is a typical self-pollinating crop with obvious heterosis in ***,the use of morphological markers during the pepper seedling stage is crucial for pepper *** color of hypocotyl is widely used as a phenotypic marker in crossing studies of *** accessions generally have purple hypocotyls,which are mainly due to the anthocyanin accumulation in seedlings,and green hypocotyls are rarely observed in *** we reported the characterization of a green hypocotyl mutant of pepper,Cha1,which was identified from a pepper ethyl methanesulfonate(EMS)mutant *** mapping revealed that the causal gene,CaTTG1,belonging to the WD40 repeat family,controlled the green hypocotyl phenotype of the ***-induced gene silencing(VIGS)confirmed that CaTTG1 regulated anthocyanin ***-seq data showed that expression of structural genes CaDFR,CaANS,and CaUF3GT in the anthocyanin biosynthetic pathway was significantly decreased in Cha1 compared to the wild *** two-hybrid(Y2H)experiments also confirmed that CaTTG1 activated the synthesis of anthocyanin structural genes by forming a MBW complex with CaAN1 and *** summary,this study provided a green hypocotyl mutant of pepper,and the Kompetitive Allele Specific PCR(KASP)marker developed based on the mutation site of the underlying gene would be helpful for pepper breeding.

关键词： Pepper CaTTG1 Anthocyanin Hypocotyl color Fine-mapping

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OsNCED3 and OsPYL1 promote the closure of rice florets by regulating sugar transporters through endogenous abscisic acid

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Journal of Integrative Agriculture 2025年第2期24卷 441-452页

作者： Xiawan Zhai Wenbin Kai Youming Huang Jinyin Chen Xiaochun Zeng Jiangxi Key Laboratory of Crop Physiology Ecology and Genetic BreedingJiangxi Agricultural University college of Agronomy Jiangxi Agricultural University college of Life Science and Resources and Environment Yichun University

Rice is the world's largest food crop, but it often encounters flowering asynchronization problems during hybrid rice seed production. In addition, the slow closure of female florets leads to seed mildew and affects the quality. The hormone abscisic acid(ABA) plays a crucial role in plant responses to abiotic stresses. Previous studies showed that exogenous ABA promotes floret closure, although the molecular mechanisms and effects of endogenous ABA on floret closure remain unknown. In this study, the effect of endogenous ABA on floret closure and the molecular mechanism by which ABA promotes floret closure through sugar transporters were investigated by changing the expression levels of OsNCED3 and OsPYL1 in rice. The results showed that overexpression(OE)-OsNCED3increased the endogenous ABA level of florets. Florets closed 5.91 min earlier and OsNCED3 gene knockout line delayed the closure of florets by 5.08 min compared with the wild type. In addition, OsPYL1 regulated the endogenous ABA content and changed the sensitivity to ABA such that the floret closure times for OE and CRISPR-Cas9(CR) were 9.84 min earlier and 12.78 min later, respectively, resulting in an increase in the split husk rate to 15.4%. The gene expression levels of some sugar transporters(STs) changed. The OsPYL1 and OsSWEET4proteins could interact on the cell membrane. These results indicate that ABA promotes the closure of rice florets and the enhanced sensitivity to ABA promotes this effect even more. The molecular mechanism is mainly related to downstream sugar transporters that respond to the ABA signaling pathway, especially OsSWEET4.

关键词： rice floret closure sugar transporter abscisic acid seed quality

Two APETALA2/ETHYLENE RESPONSE FACTORS coordinately with Ca MYC2 positively regulate capsaicinoid biosynthesis in pepper(Capsicum annuum)

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Horticultural Plant Journal 2025年第1期11卷 275-289页

作者： Jiali Song Renjian Liu Guoju Chen Jianjun Lei Zuoyang Ning Xiangru Tang Xiaowan Xu Muxi Chen Bihao Cao Changming Chen Zhangsheng Zhu Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsCollege of HorticultureSouth China Agricultural UniversityGuangzhouGuangdong 510642China Guangdong Vegetable engineering and Technology Research Center South China Agricultural UniversityGuangzhouGuangdong 510642China Henry Fok college of Biology and Agriculture Shaoguan UniversityShaoguanGuangdong 512005China college of Agriculture South China Agricultural UniversityGuangzhouGuangdong510642China Guangdong Key Laboratory for New Technology Research of Vegetables Vegetable Research InstituteGuangdong Academy of Agricultural SciencesGuangzhouGuangdong 510640China Guangdong Helinong seeds CO.LTD.ShantouGuangdong 515800China

The transcriptional cascade and regulatory loop play crucial roles in regulating plant-specialized metabolite *** are unique to the genus Capsicum and confer a pungent flavor to its ***,the transcriptional regulation of capsaicinoid biosynthesis remains largely *** this study,two AP2/ERF transcription factors(TFs),CaERF102 and CaERF111,were characterized for their role in the capsaicinoid biosynthesis *** analysis of two ERFs and capsaicinoid biosynthetic genes(CBGs)suggested that they were associated with capsaicinoid *** ERFs encode nuclear-localized proteins and function as transcriptional activators through their C-terminal activation *** two ERF TFs participated in capsaicinoid biosynthesis by directly activating the promoters of key CBGs,and this activation was significantly enhanced when CaMYC2 was ***,CaERF102 and CaERF111 were found to interact with *** study helps elucidate the AP2/ERF TF regulatory network that governs capsaicinoid biosynthesis in Capsicum species.

关键词： Capsicum Specialized metabolites Pungency Transcription factor AP2/ERF MYC

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Epigenetics in the modern era of crop improvements

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Science China Life Sciences 2025年

作者： Yan Xue Xiaofeng Cao Xiangsong Chen Xian Deng Xingwang Deng Yong Ding Aiwu Dong Chengguo Duan Xiaofeng Fang Lei Gong Zhizhong Gong Xiaofeng Gu Chongsheng He Hang He Shengbo He Xinjian He Yan He Yuehui He Guifang Jia Danhua Jiang Jianjun Jiang Jinsheng Lai Zhaobo Lang Chenlong Li Qing Li Xingwang Li Bao Liu Bing Liu Xiao Luo Yijun Qi Weiqiang Qian Guodong Ren Qingxin Song Xianwei Song Zhixi Tian Jiawei Wang Yuan Wang Liang Wu Zhe Wu Rui Xia Jun Xiao Lin Xu Zhengyi Xu Wenhao Yan Hongchun Yang Jixian Zhai Yijing Zhang Yusheng Zhao Xuehua Zhong Daoxiu Zhou Ming Zhou Yue Zhou Bo Zhu Jiankang Zhu Qikun Liu college of Life Sciences Institute of Life Science and Green Development Hebei University Biotechnology Research Institute Chinese Academy of Agricultural Sciences college of Biology Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation Hunan Engineering and Technology Research Center of Hybrid Rapeseed Hunan University Institute of Advanced agricultural Sciences School of Advanced Agricultural Sciences Peking University State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University National Institute of Biological Sciences Key Laboratory of seed Innovation Institute of Genetics and Developmental Biology Chinese Academy of Sciences School of Advanced agricultural Sciences Peking-Tsinghua Center for Life Sciences Peking University Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular Sciences Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education College of Chemistry and Molecular Engineering Peking University Key Laboratory of seed Innovation State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences State Key Laboratory of Wheat Improvement Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences in Weifang State Key Laboratory of Crop Stress Adaptation and Improvement School of Life Sciences Henan University State Key Laboratory of Maize Bio-breeding National Maize Improvement Center Department of Plant Genetics and Breeding China Agricultural University Frontiers Science Center for Molecular Design Breeding China Agricultural University Center for Crop Functional Genomics and Molecular Breeding China Agricultural University Sanya Institute of China agricultural University Hainan Yazhou Bay seed Laboratory Institute of Advan

Epigenetic mechanisms are integral to plant growth, development, and adaptation to environmental stimuli. Over the past two decades,our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body of knowledge on both locus-specific mechanisms and genome-wide regulatory patterns. Studies initially grounded in the model plant Arabidopsis have been broadened to encompass a diverse array of crop species, revealing the multifaceted roles of epigenetics in physiological and agronomic traits. With recent technological advancements, epigenetic regulations at the single-cell level and at the large-scale population level are emerging as new focuses. This review offers an in-depth synthesis of the diverse epigenetic regulations, detailing the catalytic machinery and regulatory functions. It delves into the intricate interplay among various epigenetic elements and their collective influence on the modulation of crop traits. Furthermore, it examines recent breakthroughs in technologies for epigenetic modifications and their integration into strategies for crop improvement. The review underscores the transformative potential of epigenetic strategies in bolstering crop performance, advocating for the development of efficient tools to fully exploit the agricultural benefits of epigenetic insights.

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Development of an Agrobacterium tumefaciens-mediated transformation system for somatic embryos and transcriptome analysis of LcMYB1's inhibitory effect on somatic embryogenesis in Litchi chinensis

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Journal of Integrative Agriculture 2025年第2期24卷 594-609页

作者： Yaqi Qin Bo Zhang Xueliang Luo Shiqian Wang Jiaxin Fu Zhike Zhang Yonghua Qin Jietang Zhao Guibing Hu State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China) Ministry of Agriculture and Rural Affairs/Guangdong Litchi Engineering Research CenterCollege of HorticultureSouth China Agricultural University

Litchi has great economic significance as a global fruit crop. However, the advancement of litchi functional genomics has encountered substantial obstacles due to its recalcitrance to stable transformation. Here, we present an efficacious Agrobacterium tumefaciens-mediated transformation system in somatic embryos of ‘Heiye' litchi. This system was developed through the optimization of key variables encompassing explant selection, A. tumefaciens strain delineation, bacterium concentration, infection duration, and infection methodology. The subsequent validation of the transformation technique in litchi was realized through the ectopic expression of LcMYB1, resulting in the generation of transgenic calli. However, the differentiation of transgenic calli into somatic embryos encountered substantial challenges. To delineate the intricate molecular underpinnings of LcMYB1's inhibitory role in somatic embryo induction, a comprehensive transcriptome analysis was conducted that encompassed embryogenic calli(C), globular embryos(G), and transgenic calli(TC). A total of 1,166 common differentially expressed genes(DEGs) were identified between C-vs.-G and C-vs.-TC. Gene Ontology(GO) annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis revealed that these common DEGs were mostly related to plant hormone signal transduction pathways. Furthermore, RT-qPCR corroborated the pronounced down-regulation of numerous genes that are associated with somatic embryo induction within the transgenic calli. The development of this transformation system provides valuable support for functional genomics research in litchi.

关键词： Litchi chinensis A. tumefaciens transformation LcMYB1 somatic embryo

The NAC transcription factor MdNAC4 positively regulates nitrogen deficiency‑induced leaf senescence by enhancing ABA biosynthesis in apple

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Molecular Horticulture 2023年第1期3卷 337-356页

作者： Binbin Wen Xuehui Zhao Xingyao Gong Wenzhe Zhao Mingyue Sun Xiude Chen Dongmei Li Ling Li Wei Xiao State Key Laboratory of Crop Biology College of Horticulture Science and EngineeringShandong Agricultural UniversityTaian 271018ShandongChina college of seed and facility agricultural engineering Weifang UniversityWeifang 261061ShandongChina

Although it is well established that nitrogen(N)deficiency induces leaf senescence,the molecular mechanism of N deficiency-induced leaf senescence remains largely ***,we show that an abscisic acid(ABA)-responsive NAC transcription factor(TF)is involved in N deficiency-induced leaf *** overexpression of MdNAC4 led to increased ABA levels in apple calli by directly activating the transcription of the ABA biosynthesis gene *** addition,MdNAC4 overexpression promoted N deficiency-induced leaf *** investigation showed that MdNAC4 directly bound the promoter of the senescence-associated gene(SAG)MdSAG39 and upregulated its ***,the function of MdNAC4 in promoting N deficiency-induced leaf senescence was enhanced in the presence of ***,we identified an interaction between the ABA receptor protein MdPYL4 and the MdNAC4 ***,MdPYL4 showed a function similar to that of MdNAC4 in ABA-mediated N deficiencyinduced leaf *** findings suggest that ABA plays a central role in N deficiency-induced leaf senescence and that MdPYL4 interacts with MdNAC4 to enhance the response of the latter to N deficiency,thus promoting N deficiency-induced leaf *** conclusion,our results provide new insight into how MdNAC4 regulates N deficiency-induced leaf senescence.

关键词： Apple MdNAC4 MdPYL4 N deficiency ABA Leaf senescence

Design and parameter optimization of flexible comb-type grass seed metering device

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International Journal of agricultural and Biological engineering 2015年第1期8卷 9-16页

作者： Yang Song Zhang Shumin college of engineering Nanjing Agricultural UniversityNanjing 210031China college of engineering China Agricultural UniversityBeijing 100083China

In order to optimize the structure schema of flexible comb-type grass seeder,the effects of combing teeth gap L,rotation speed of comb shaft N,seed metering device inclining angle α and groove strip inclining angle θ on the rate and uniformity of seeding were observed by test and analyzed,then the coefficient of seeding stability variation was explored in the simulating planting environment on the soil tank test bed based on the above four influencing factors,with Festuca arundinacea seeds as *** to the results of regression analysis,the model fits well with the actual situation.L,N,α,and the interaction term of N and θ are significant,and the interaction term of θ and α is extremely *** analysis of interaction factor effect shows that the variation coefficient responds to the interaction of N and θ in the same manner as that responds to the interaction of θ and α,showing ‘saddle’ variation *** the acceptable maximum value of coefficient of seeding uniformity variation is 4%,then the inverse proportion relationship between factors is inferred according to the change trend of the above interaction factors and derivation of regression *** the regression equation was simplified according to the inverse proportion equation,and four groups of parameters were *** parameter group was tested,and the test results agreed with the regression equation's predicting value,with the correlation coefficient *** research can provide reference for the development of seed metering devices and grass seeders.

关键词： grass seeder structural design parameter optimization agricultural machinery flexible structures

Mapping of fruit apex shape related QTLs across multi-genetic backgrounds in cucumber(Cucumis sativus L.)

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Horticultural Plant Journal 2022年第3期8卷 328-340页

作者： Pinyu Zhu Yongjiao Meng Kaijing Zhang Xueyan Wang Kun Liang Tuantuan Wang Jian Xu Xiaodong Qin Zhe Wu Chunyan Cheng Xiaqing Yu Chuntao Qian Qunfeng Lou Jinfeng Chen Ji Li State Key Laboratory of Crop Genetics and Germplasm Enhancement College of HorticultureNanjing Agricultural UniversityNanjing 210095China college of engineering/Jiangsu Province engineering Lab for Modern facility Agriculture Technology&Equipment Nanjing Agricultural UniversityNanjing 210031China college of Agriculture Anhui Science and Technology UniversityFengyang 233100China

The shape of fruit apex is critical to appearance quality in cucumber(Cucumis sativus L.),of which the genetic basis was poorly understood,and the use of marker-assisted breeding for fruit apex improvement is not available *** this study,the variation of fruit apex in different cucumber ecotypes was evaluated by fruit apex angle(variation coefficient from 7.1%to 15.7%)and fruit apex index(variation coefficient from 8.8%to 22.6%).Fruit apex associated QTLs were mapped by using 145 F_(2:3) families and 155 F_(2:6) population that were derived from the cross of different ecotype *** of the mapping populations were conducted in four experiments in 2 *** majoreffect QTLs,Bfal4.1,Bfai4.1,Bfad6.1 and Bfai6.1 were consistently and reliably detected across two environments which could explain 11.6%-33.6%phenotypic variations(R^(2))in the F_(2:3) *** major-effect QTLs,Ofai4.1(R^(2)=13.4%-15.5%),Ofal4.1(R^(2)=10.7%-12.8%),and Ofad6.1(R^(2)=11.6%-12.4%)were stably detected in the F_(2:6) population in two ***4.1,Bfal4.1,Ofai4.1 and Ofal4.1 were integrated to be consensus QTL fa4.1,within which 11 candidate genes were ***6.1 and Bfad6.1 were integrated to be consensus QTL *** interaction analysis showed that Bfai6.1 has epistatic effect with *** study revealed two reliable major-effect fruit apex related QTLs across multi-genetic backgrounds and environments in *** possible candidate genes regulating the shape of fruit apex,and the relationship between cell division and fruit apex morphogenesis were discussed.

关键词： Cucumber Fruit apex Variation QTL Candidate gene

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Two-stage seedling cultivation method for sweet peppers combining closed plant factory and solar greenhouse

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International Journal of agricultural and Biological engineering 2023年第6期16卷 56-66页

作者： Hongbo Cui Fang Ji Yanni Liu Zhengyang Luo Xiufeng Wang Jianfeng Wang Key Laboratory of facility Vegetable of Jilin Province Jilin Academy of Vegetable and Flower SciencesChangchun 130119China Key Laboratory of agricultural engineering in Structure and Environment of MARA College of Water Resources&Civil EngineeringChina Agricultural UniversityBeijing 100083China college of Horticulture Jilin Agricultural UniversityChangchun 130033China

This study introduced a two-stage cultivation method for sweet pepper seedlings, integrating the strengths of a closed plant factory and solar greenhouse, to mitigate the environmental constraints in Northeast China during the early spring season. In the first stage, seedlings were cultivated in a closed plant factory, followed by a second stage in a solar greenhouse. Four treatments- T1 (9 and 36 d), T2 (12 and 33 d), T3 (15 and 30 d), and T4 (18 and 27 d) - were designed for the first and second stages, respectively, with solar greenhouse-only approach serving as the control (CK). The findings reveal that the two-stage methodology significantly outperformed the control across multiple metrics, including seedling health index, chlorophyll content, photosynthetic capacity, yield, etc. Specifically, T3 emerged as optimal, boosting the health index by 38.59%, elevating chlorophyll content by 39.61%, increasing net photosynthesis by 34.61%, and augmenting yield per plant by 40.67%. Additionally, T3 expedited the time to harvest by 25 d compared to the control. Although the seedling cost for T3 was 0.12 RMB yuan higher, the benefits offset the additional investment. In conclusion, the two-stage cultivation method effectively leverages the advantages of both closed-plant factories and solar greenhouses, resulting in superior seedling quality compared to using only solar greenhouses. It offers a practical and economically viable solution for enhancing the quality and yield of sweet pepper seedlings, thus contributing to the progress in the field of facility seedling cultivation research.

关键词： two-stage seedling cultivation sweet peppers closed plant factory solar greenhouse