The NAC transcription factor MdNAC4 positively regulates nitrogen deficiency‑induced leaf senescence by enhancing ABA biosynthesis in apple

作     者：Binbin Wen Xuehui Zhao Xingyao Gong Wenzhe Zhao Mingyue Sun Xiude Chen Dongmei Li Ling Li Wei Xiao 

作者机构：State Key Laboratory of Crop BiologyCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTaian 271018ShandongChina College of Seed and Facility Agricultural EngineeringWeifang UniversityWeifang 261061ShandongChina 

出 版 物：《Molecular Horticulture》 (分子园艺（英文）)

年 卷 期：2023年第3卷第1期

页      面：337-356页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：Open access funding provided by Shanghai Jiao Tong University funded by Shandong Province Major Science and Technology Innovation Project(2018CXGC0209) Shandong Provincial Fruit Industry Technology System-Cultivation and Soil Fertilization Post(SDAIT-06-04) Natural Science Foundation of Shandong Provincial(ZR2020ZD18) 

主　　题：Apple MdNAC4 MdPYL4 N deficiency ABA Leaf senescence 

摘      要：Although it is well established that nitrogen(N)deficiency induces leaf senescence,the molecular mechanism of N deficiency-induced leaf senescence remains largely ***,we show that an abscisic acid(ABA)-responsive NAC transcription factor(TF)is involved in N deficiency-induced leaf *** overexpression of MdNAC4 led to increased ABA levels in apple calli by directly activating the transcription of the ABA biosynthesis gene *** addition,MdNAC4 overexpression promoted N deficiency-induced leaf *** investigation showed that MdNAC4 directly bound the promoter of the senescence-associated gene(SAG)MdSAG39 and upregulated its ***,the function of MdNAC4 in promoting N deficiency-induced leaf senescence was enhanced in the presence of ***,we identified an interaction between the ABA receptor protein MdPYL4 and the MdNAC4 ***,MdPYL4 showed a function similar to that of MdNAC4 in ABA-mediated N deficiencyinduced leaf *** findings suggest that ABA plays a central role in N deficiency-induced leaf senescence and that MdPYL4 interacts with MdNAC4 to enhance the response of the latter to N deficiency,thus promoting N deficiency-induced leaf *** conclusion,our results provide new insight into how MdNAC4 regulates N deficiency-induced leaf senescence.