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Identification and functional verification of Y-chromosome-specific gene typo-gyf in Bactrocera dorsalis

Insect Science 2024年第4期31卷 1270-1284页

作者： Peipei Liu Shuning Yu Wenping Zheng Qiuyuan Zhang Jiao Qiao Ziniu Li Zhurong Deng Hongyu Zhang National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable horticultural Crops Hubei Hongshan LaboratoryChina-Australia Joint Research Centre for Horticultural and Urban PestsInstitute of Urban and Horticultural EntomologyCollege of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina

Genes on the Y chromosome play important roles in male sex determination and *** identification of Y-chromosome-specific genes not only provides a theoretical basis for the study of male reproductive development,but also offers genetic control targets for agricultural ***,Y-chromosome genes are rarely characterized due to their high repeatability and high heterochromatinization,especially in the oriental fruit *** this study,1011 Y-chromosome-specific candidate sequences were screened from 2 to 4 h Bactrocera dorsalis embryo datasets with the chromosome quotient method,6 of which were identified as Y-chromosome-specific sequences by polymerase chain reaction,including typo-gyf,a 19126-bp DNA sequence containing a 575-amino acid open reading *** deformation and a significant reduction in sperm number were observed after typo-gyf knockdown with RNA interference in *** typo-gyf knockout with clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9 in the embryonic stage,the sex ratio of the emergent adults was unbalanced,with far more females than males.A genotype analysis of these females with the Y-chromosome gene MoY revealed no sex ***-gyf knockout led to the death of XY individuals in the embryonic *** conclude that typo-gyf is an essential gene for male survival,and is also involved in testicular development and *** identification of typo-gyf and its functional verification provide insight into the roles of Y-chromosome genes in male development.

关键词： Bactrocera dorsalis spermatogenesis testis typo-gyf gene Y-specific gene

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VPD modifies CO_(2)fertilization effect on tomato plants via abscisic acid and jasmonic acid signaling pathways

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horticultural Plant Journal 2024年第5期10卷 1165-1176页

作者： Dalong Zhang Huihua Yang Xiaolu Chen Yan Li Yunzhou Li Hongye Liu Xulin Wu Min Wei college of horticultural Science and Engineering Shandong Agricultural UniversityTai'anShandong 271018China college of Agriculture Guizhou UniversityGuiyangGuizhou 550025China

Atmospheric CO_(2)concentration is elevated globally,which has“CO_(2)fertilization effects”and potentially improves plant photosynthesis,yield,and *** the beneficial effect of CO_(2)fertilization being modulated by vapor pressure deficit(VPD),the underlying mechanism is highly *** the present study,the potential roles of hormones in determining CO_(2)fertilization effects under contrasting high and low VPD conditions were investigated by integrated physiological and transcriptomic *** CO_(2)fertilization effects were offset under high VPD conditions and were constrained by plant water stress and photosynthetic CO_(2)*** VPD induced a large passive water driving force,which disrupted the water balance and consequently caused plant water *** water potential,turgor pressure,and hydraulic conductance declined under high VPD *** physiological evidence combined with transcriptomic analyses demonstrated that abscisic acid(ABA)and jasmonic acid(JA)potentially acted as drought-signaling molecules in response to high VPD *** foliar ABA and JA content triggered stomatal closure to prevent excessive water loss under high VPD stress,which simultaneously increased the diffusion resistance for CO_(2)uptake from atmosphere to leaf intercellular *** VPD also significantly increased mesophyll resistance for CO_(2)transport from stomatal cavity to fixation site inside *** chloroplast“sink”CO_(2)availability was constrained by stomatal and mesophyll resistance under high VPD stress,despite the atmospheric“source”CO_(2)concentration being ***,ABA-and JA-mediated drought-resistant mechanisms potentially modified the beneficial effect of CO_(2)fertilization on photosynthesis,plant growth,and yield *** study provides valuable information for improving the utilization efficiency of CO_(2)fertilization and a better understanding of the physiological processes.

关键词： Abscisic acid CO_(2)fertilization Jasmonic acid Mesophyll conductance Stomatal conductance Vapor pressure deficit

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Exogenous calcium enhances the physiological status and photosynthetic capacity of rose under drought stress

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horticultural Plant Journal 2024年第3期10卷 853-865页

作者： Xiaojuan Zhao Shang Lin Shuang Yu Yichang Zhang Lin Su Lifang Geng Chenxia Cheng Xinqiang Jiang college of Landscape Architecture and Forestry Qingdao Agricultural UniversityQingdaoShandong 266000China college of horticultural Qingdao Agricultural UniversityQingdaoShandong 266000China

Drought(water shortage)can substantially limit the yield and economic value of rose plants(Rosa spp.).Here,we characterized the effect of exogenous calcium(Ca^(2+))on the antioxidant system and photosynthesis-related properties of rose under polyethylene glycol 6000(PEG6000)-induced drought *** levels,as well as leaf and root biomass,were significantly reduced by drought;drought also had a major effect on the enzymatic antioxidant system and increased concentrations of reactive oxygen *** of exogenous Ca^(2+)increased the net photosynthetic rate and stomatal conductance of leaves,enhanced water-use efficiency,and increased the length and width of stomata following exposure to ***-specific physiological responses were observed under different concentrations of Ca^(2+).Application of 5 mmol·L^(-1)Ca^(2+)promoted photosynthesis and antioxidant activity in the leaves,and application of 10 mmol·L^(-1)Ca^(2+)promoted antioxidant activity in the *** of exogenous Ca^(2+)greatly enhanced the phenotype and photosynthetic capacity of potted rose plants following exposure to drought ***,our findings indicate that the application of exogenous Ca^(2+)enhances the drought resistance of roses by promoting physiological adaptation and that it could be used to aid the cultivation of rose plants.

关键词： Rosa hybrida L. Exogenous calcium Drought stress Physiological index Photosynthetic capacity

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Resistance index and browning mechanism of apple peel under high temperature stress

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horticultural Plant Journal 2024年第2期10卷 305-317页

作者： Hui Wang Shuhui Zhang Zidun Wang Dongmei Li Leiyu Yan Yifeng Feng Xiaojie Liu Rongxin Chen Wenmin Fan Lulong Sun Zhengyang Zhao college of Horticulture Northwest A&F UniversityYanglingShaanxi 712100China college of horticultural Science and Engineering Shandong Agricultural University State Key Laboratory of Crop BiologyTai'anShandong 271018China

Apples are one of the most important economic crops *** of global warming and an aggravation of environmental,abnormally high temperatures occur frequently in fruit-growing season and seriously affect normal fruit growth and reduce fruit quality and *** took five-year-old Ruixue’(Qinfu 1×Pink Lady;CNA20151469.1) fruits as test materials,and the ambient temperature during fruit development was *** results showed that during the fruit-growing season,especially during the rapid growth stage (July to August),the maximum daily temperature exceeded 30℃ and lasted for more than 40 *** determine the effects of high temperature stress on the apple fruit resistance,we treated expanding,veraison,and maturity-period fruits at different *** was found that the fruits of the expanding period showed strong resistance to high temperature stress,whereas during veraison and maturity,fruit resistance to high temperature stress decreased,and the fruit peel browning phenotype ***,the content of malonaldehyde (MDA),hydrogen peroxide (H_(2)O_(2)),and superoxide anion (O._(2)^(-)) in the peel gradually increased with increasing *** content of total phenols,flavanol,and flavonoids in the peel decreased substantially at 45℃.Moreover,it was found that polyphenol oxidase gene (MdPPO1) was most sensitive to high temperature stress in ***,transient and stable MdPPO1 overexpression significantly promoted peel *** transgenic materials were more sensitive to high temperatures,and browning was more severe compared to non-genetically modified organism (WT).Stable MdPPO1 knockout calli obtained via clustered regularly interspersed short palindromic repeats (CRISPR/Cas9) gene knockout technology reduced the browning phenotype,and the resultant fruits were not sensitive to the effects of high temperature ***,MdPPO1 expression may be a key factor of high temperature-related changes observed in the browni

关键词： Malus domestica Borkh High temperature stress Peel Browning MdPPO1

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Identification of the lysine and histidine transporter family in Camellia sinensis and the characterizations in nitrogen utilization

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horticultural Plant Journal 2024年第1期10卷 273-287页

作者： Wei Huang Danni Ma Fawad Zaman Xulei Hao Li Xia E Zhang Pu Wang Mingle Wang Fei Guo Yu Wang Dejiang Ni Hua Zhao National Key Laboratory for Germplasm Innovation&Utilization of horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China

In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino *** tea plant(Camellia sinensis)is a perennial evergreen with a relatively high level of amino ***,systematic identification and molecular characterization of the LHT gene family has rarely been reported in tea *** this study,22 CsLHTs were identified from the‘Shuchazao’genome and classified into two *** modeled three-dimensional structure and the conserved domains presented a high similarity among the LHTs ***,it was predicted that a few genes were conserved through the analysis of the physiochemical characters,structures and cis-elements in *** expression patterns in tea plants revealed that CsLHT7 was mainly expressed in the roots,and CsLHT4 and CsLHT11 exhibited relatively high expression in both the roots and ***,the expression of all three genes could be induced by organic ***,heterogeneous expression of CsLHT4,CsLHT7 and CsLHT11 in Arabidopsis thaliana decreased the aerial parts biomass compared with that in WT plants while significantly increased the rosette biomass only for CsLHT11transgenic plants versus WT ***,our results provide fundamental information about CsLHTs and potential genes in N utilization for further analysis in tea plants.

关键词： Camellia sinensis Nitrogen Lysine and histidine transporter(LHT)family

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Mutation in the Agrobacterium his I gene enhances transient expression in pepper

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horticultural Plant Journal 2024年第3期10卷 809-822页

作者： Dan Liu Shengnan Zhao Jubin Wang Xi Zhang Yingtian Deng Feng Li National Key Laboratory for Germplasm Innovation&Utilization of horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China

Agrobacterium-mediated plant transformation is widely used in plant genetic ***,its efficiency is limited by plant immunity against *** pepper(Capsicum annuum L.)is an important vegetable that is recalcitrant to Agrobacterium-mediated *** this work,Agrobacterium was found to induce a strong immune response in pepper,which might be the reason for T-DNA being difficult to express in *** Agrobacterium mutant screen was conducted and a point mutation in the hisI gene was identified due to a weak immune response and enhanced transient expression mediated by this Agrobacterium mutant in pepper *** genetic analysis revealed that histidine biosynthesis deficiency caused by mutations in many genes of this pathway led to reduced pepper cell death,presumably due to reduced bacterial ***,mutation analysis of threonine and tryptophan biosynthesis genes showed that the biosynthesis of different amino acids may play different roles in Agrobacterium growth and stimulating the pepper immune *** possible application of Agrobacterium amino acid biosynthesis mutations in plant biology was discussed.

关键词： Capsicum annuum L. Agrobacterium Transient transformation efficiency Mutation Amino acid Immune response

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Biochar alleviates apple replant disease by reducing the growth of Fusarium oxysporum and regulating microbial communities

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horticultural Plant Journal 2024年第3期10卷 657-671页

作者： Yinghao Liu Can Wang Ran Chen Weitao Jiang Yun Li Chengmiao Yin Yanfang Wang Zhiquan Mao college of Chemistry and Material Science Shandong Agricultural UniversityTaianShandong 271018China State Key Laboratory of Crop Biology/college of horticultural Science and Engineering Shandong Agricultural UniversityTaianShandong 271018China

Apple replant disease(ARD)negatively affects plant growth and reduces yields in replanted *** this study,biochar was applied to apple replant soil with Fusarium *** aim was to investigate whether biochar could promote plant growth and alleviate apple replant disease by reducing the growth of harmful soil microorganisms,changing soil microbial community structure and improving the soil *** experiment included five treatments:apple replant soil(CK),methyl bromide fumigation apple replant soil(FM),replant soil with biochar addition(2%),replant soil with *** spore solution(8×10^(7)spores·mL^(-1)),and replant soil with biochar and *** spore solution *** biomass,the activity of antioxidant enzymes in the leaves and roots,and soil environmental variables were *** community composition and community structure were analyzed using 16SrDNA and ITS2 gene *** significantly reduced the abundance of *** and increased soil microbial diversity and *** also increased the soil enzyme activities(urease,invertase,neutral phosphatase,and catalase),the biomass(plant height,fresh weight,dry weight)and the activity of antioxidant enzymes(superoxide dismutase,peroxidase,and catalase).The root indexes of apple seedlings was also increased in replant soil by *** sum,biochar promoted the growth of plants,improved the replant soil environment,and alleviated apple replant disease.

关键词： Biochar Fusarium oxysporum Apple replant disease Soil environment

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Identification,characterization,and verification of miR399 target gene in grape

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horticultural Plant Journal 2024年第1期10卷 91-102页

作者： Maosong Pei Hainan Liu Tonglu Wei Huiying Jin Yihe Yu Mengting Ma Xiaomeng Song Rundong Dai Dalong Guo Henan Engineering Technology Research Center of Quality Regulation and Controlling of horticultural Plants College of Horticulture and Plant ProtectionHenan University of Science and TechnologyLuoyangHenan 471023China

The microRNA miR399 plays an important role in phosphorus signal transduction pathways in ***,miR399 was shown to be closely associated with berry ripening in grape(Vitis vinifera).The objective of the present study was to elucidate the evolutionary characteristics of the miR399 gene family in grape and to verify the cleavage effect on the target *** miR399s were identified by miRNA sequencing and retrieval from the miRBase *** mature sequences and precursor sequences were subjected to phylogenetic analysis to reconstruct evolutionary trees,as well as secondary structure analysis of the precursor sequence,and prediction of target *** cisacting elements in the miR399 promoter were predicted and the cleavage effect of grape miR399b on its target genes was *** grape miR399 family comprised nine precursor sequences and nine mature *** precursor sequences formed a typical and stable stem—loop *** minimum folding free energy ranged from-55.70 kcal·mol^(-1)to-37.40 kcal·mol^(-1).Multiple sequence alignment revealed that the miR399 family was highly *** grape miR399 family was phylogenetically closely related to peach,apple,and citrus *** miR399s were predicted to target inorganic phosphate transporter 1—3,phospholipase D delta-like,and *** cleavage effect of grape miR399b on the target genes was verified by means of a dual-luciferase assay and 5’*** GUS staining showed that the promoter activity of miR399b was promoted by GA3treatment.

关键词： Grape miR399 Phosphorus metabolism Early ripening

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Transcription factors involved in tomato fruit quality improvement

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Food Quality and Safety 2024年第1期8卷 50-54页

作者：石艳娜戴正蓉陈昆松 college of Agriculture and Biotechnology Zhejiang UniversityZijingang CampusHangzhouChina Zhejiang Provincial Key Laboratory of horticultural Plant Integrative Biology Zhejiang UniversityZijingang CampusHangzhouChina State Agriculture Ministry Laboratory of horticultural Plant Growth Development and Quality ImprovementZhejiang UniversityZijingang CampusHangzhouChina

Fruit quality is both a consumer demand and an important goal for horticultural *** exploration of quality-related genes provides targets to create high-quality germplasm using biotechnology,of which transcription factors(TFs)exhibit dosage effects compared with structural ***,an important horticultural crop as well as model fruit,has been widely ***,we focused on TFs with potential utilization value that control appearance(***,pigment)and internal(***,texture,shelf life and health benefits)quality in ***,potential strategies to generate high-quality tomato fruit are discussed.

关键词： Fruit quality transcription factor tomato.

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Improved genome annotation of Brassica oleracea highlights the importance of alternative splicing

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horticultural Plant Journal 2024年第4期10卷 961-970页

作者： Yinqing Yang Lei Zhang Qi Tang Lingkui Zhang Xing Li Shumin Chen Kang Zhang Ying Li Xilin Hou Feng Cheng State Key Laboratory of Crop Genetics and Germplasm Enhancement College of HorticultureNanjing Agricultural UniversityNanjing 210095China State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural AffairsSino-Dutch Joint Laboratory of Horticultural GenomicsBeijing 100081China

Brassica oleracea has been developed into many important crops,including cabbage,kale,cauliflower,broccoli and so *** genome and gene annotation of cabbage(cultivar JZS),a representative morphotype of ***,has been widely used as a common reference in biological *** its genome assembly has been updated twice,the current gene annotation still lacks information on untranslated regions(UTRs)and alternative splicing(AS).Here,we constructed a high-quality gene annotation(JZSv3)using a full-length transcriptome acquired by nanopore sequencing,yielding a total of 59452 genes and 75684 ***,we re-analyzed the previously reported transcriptome data related to the development of different tissues and cold response using JZSv3 as a reference,and found that 3843 out of 11908 differentially expressed genes(DEGs)underwent AS during the development of different tissues and 309 out of 903 cold-related genes underwent AS in response to cold ***,we also identified many AS genes,including BolLHCB5 and BolHSP70,that displayed distinct expression patterns within variant transcripts of the same gene,highlighting the importance of JZSv3 as a pivotal reference for AS ***,JZSv3 provides a valuable resource for exploring gene function,especially for obtaining a deeper understanding of AS regulation mechanisms.

关键词： Brassica oleracea Oxford nanopore technologies Gene annotation Alternative splicing

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