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Music stimulus has a positive eff ect on survival and development of the larvae in swimming crab Portunus trituberculatus

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Journal of Oceanology and Limnology 2022年第3期40卷 1277-1285页

作者： Zhiming REN Jiechi WANG Chunlin WANG Changkao MU Yangfang YE Ce SHI Key Laboratory of Applied marine Biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China collaborative innovation center for zhejiang marine high-effi ciency and healthy aquaculture Ningbo 315211China

The sound stimulus has a signifi cant impact on marine ***,many studies have focused on the negative impacts of sound on marine *** positive impacts of sound on marine animals,especially on marine invertebrates,were rarely *** the present study,we evaluated the eff ects of music on the survival and development of an economically important marine invertebrate,swimming crab Portunus *** pieces of classical music and a control group,i.e.,Eine Kleine Nachtmusik by Mozart(EK),Bach-Violin Concerto No.1(BV),Anonymous Romanza by Anonymous(AR),and environmental sound(EN)were *** sound pressure received were 163-dB root mean square(RMS)re 1μPa for EK,166-dB RMS re 1μPa for BV,165-dB RMS re 1μPa for AR,44-dB RMS re 1μPa for EN,*** larvae were subject to 3-h classical music transmission every day during the entire larval development *** result showed that BV and AR signifi cantly elevated the cumulative survival rate of the larva from 3.42%±1.77%(EN)to 10.65%±1.69%(BV)and 7.12%±1.37%(AR)respectively(P<0.05).Though music did not aff ect the cumulative development time of larvae from ZoeaⅠto ZoeaⅣ,it signifi cantly increased the molting rate of megalopa from 24.00%±11.78%(EN)to 44.00%±13.06%(AR)and 46.67%±10.50%(BV)(P<0.05).Musical exposure during critical growth intervals may contribute to the survival and development of larval *** an application point of view,music intervention is much economically *** observed music eff ects imply that music could further enhance growth,welfare,and production.

关键词： swimming crab Portunus trituberculatus sound classical music oxygen consumption

Expression and function of WNT4 involved in larvae development and limb regeneration in Portunus trituberculatus

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Journal of Oceanology and Limnology 2021年第1期39卷 306-316页

作者： Zhiming REN Yuanyuan FU Lei LIU Xiao LIU Chunlin WANG Key Laboratory of Applied marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315832China collaborative innovation center for zhejiang marine high-effi ciency and healthy aquaculture Ningbo UniversityNingbo 315832China

The wingless-related integration site(WNT)proteins are a family of secreted glycoproteins that are evolutionarily conserved and are believed to be involved in evolution in vertebrates and *** signaling pathways may be associated with limb regeneration and development in ***,the detail mechanisms remain ***,the distribution of WNT4 in the hepatopancreas,muscle,hemocyte,ganglion,heart,eyestalk,gill tissue,and diff erent larvae development stages of the swimming crab(Portunus trituberculatus)were characterized using immunofl uorescence,real-time PCR,and Western *** cant PtWNT4 expression was detected in heart and *** addition,PtWNT4 was expressed in all larval stages of *** with a dynamic expression pattern,especially in the eyestalk and other organs in the carapace *** injection of WNT4 dsRNA into regenerative limbs signifi cantly decreased PtWNT4 mRNA levels in the eyestalk,heart,and muscle,resulting in 1.9-fold,2.2-fold,and 2.7-fold decreases compared with those detected in the group injected with crab saline(P<0.05),respectively,indicating successful gene ***,expression analysis on the WNT4 using RNAi provides an insight to its functional mechanism during limb regeneration in *** results not only demonstrated the requirement for WNT4 in limb regeneration of Crustaceans,but also suggested its ability to promote larval development at specifi c stages.

关键词： wingless-type MMTV integration site family member-4(WNT4) limb regeneration larvae development Portunus trituberculatus expression pattern RNAi

Protective Effects of Sepia Ink Melanin on Hepatic Tissue in Streptozotocin-Induced Diabetic Mice

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Journal of Ocean University of China 2022年第5期21卷 1362-1372页

作者： WANG Xiaopeng HAN Meng DONG Hui ZHAO Yun SONG Weiwei WANG Chunlin MU Changkao LI Ronghua Key Laboratory of Applied marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315832China collaborative innovation center for zhejiang marine high-efficiency and healthy aquaculture Ningbo 315832China

Diabetes mellitus(DM)has emerged as a serious public health concern,due to the high morbidity and mortality resulted from its complications,such as diabetic nephropathy,diabetic cardiovascular complication,and diabetic neuropathy,*** this study,we investigated the beneficial effects of sepia ink melanin(SIM)on hyperglycaemia and the restoration of diabetic symptoms in streptozotocin(STZ)-induced diabetic model *** first,the normal experimental mice were performed with intraperitoneal injection of STZ(40 mg(kg BW)^(−1))(BW,body weight)to attain diabetes and then were treated with different concentrations of SIM(120,240 and 480 mg(kg BW)^(−1))for four *** treatment,significant decrease in gluconeogenesis were determined,accompanied by a notable increase in both glycolysis and oxidative enzyme activities in SIM-treated groups,such as liver marker enzymes in the serum and key antioxidant enzymes in *** results revealed the transcriptional alterations in SIM-treated *** exposure increased the expression levels of several genes related to insulin transduction and PI3K/Akt pathway,including PI3K,Akt,Irs-2,and ***,expression levels of Dicarbonyl/l-xylulose reductase(Dcxr)and UDP-glucose dehydrogenase(Ugdh),which are in-volved in pentose-glucuronate interconversion pathway,were also elevated in SIM-treated ***,histological observation results indicated that nuclear deformation and organelle dissolution were improved,thus could enhance the liver *** results demonstrated that SIM can be effective in ameliorating diabetic symptoms and improving disease management for diabetic patients.

关键词： diabetic hepatopathy sepia ink melanin PI3K/Akt pathway pentose-glucuronate interconversion pathway

Disruption of bacterial balance in the gut of Portunus trituberculatus induced by Vibrio alginolyticus infection

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Journal of Oceanology and Limnology 2018年第5期36卷 1891-1898页

作者： XIA Mengjie PEI Feng MU Changkao YE Yangfang WANG Chunlin Key Laboratory of Applied marine Biotechnology（Ningbo University） Ministry of EducationNingbo 315211China collaborative innovation center for zhejiang marine high-efficiency and healthy aquaculture Ningbo 315211China

A bstract Gut microbiota impacts the health of crustaceans. V ibrio alginolyticus is a main causative pathogen that induces the vibriosis in farmed swimming crabs, Portunus trituberculatus. However, it remains unknown whether gut bacteria perform functions during the progression of vibriosis. In this study, 16 SrRNA gene amplicon sequencing was used to investigate temporal alteration of gut bacterial community in swimming crabs in response to 72-h V. alginolyticus challenge. Our results show that V. alginolyticus infection resulted in dynamic changes of bacterial community composition in swimming crabs. Such changes were highlighted by the overwhelming overabundance of V ibrio and a significant fluctuation in the gut bacteria including the bacteria with high relative abundance and especially those with low relative abundance. These findings reveal that crab vibriosis gradually develops with the infection time of V. alginolyticus and tightly relates to the dysbiosis of gut bacterial community structure. This work contributes to our appreciation of the importance of the balance of gut bacterial community structure in maintaining the health of crustaceans.

关键词： Portunus trituberculatus Vibrio alginolyticus gut bacterial community composition 16S rRNA gene amplicon sequencing

Phospholipid Compositions in Portunus trituberculatus Larvae at Different Developmental Stages

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Journal of Ocean University of China 2022年第1期21卷 152-162页

作者： LU Zhibin SHI Ce LIU Lei MU Changkao YE Yangfang WANG Chunlin Key Laboratory of Applied marine Biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China collaborative innovation center for zhejiang marine high-efficiency and healthy aquaculture Ningbo 315211China

Phospholipids are used to improve the growth and survival of Portunus trituberculatus,a widely cultured crab species in ***,only total phospholipids or several classes are applied in crab *** this study,we employed a targeted lipidomic method to investigate the comprehensive phospholipid composition in *** larvae and reveal the changing phospholipid profile over the larval *** showed that *** larvae contain 112 phospholipid species belonging to 10 phospholipid classes,in which phosphatidylcholine(PC)and phosphatidylethanolamine(PE)species are the most abundant,and PC,PE,phosphatidic acid,and phosphatidylserine(PS)are with high *** levels of all phospholipids significantly changed with larval development,which was highlighted by the downward parabolic changes in PE,phosphatidylglycerol,phosphatidylino-sitol,PS,lysophosphatidic acid,and sphingomyelin *** addition,nearly all phospholipid species were depleted at the M stage,which probably contributed to the mass mortality of crab *** findings on the composition and alterations of phospholipids in *** larvae provide novel perspectives for the targeted supplementation of phospholipids in crab *** work also highlights the use of targeted UHPLC-MS lipidomics in understanding the changes of phospholipids during crab development.

关键词： Portunus trituberculatus larval development phospholipid UHPLC-MS

Using iTRAQ-Based Quantitative Proteomics Analysis to Identify Differentially Expressed Proteins Related to Larval Development of Portunus trituberculatus

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Journal of Ocean University of China 2021年第4期20卷 897-910页

作者： CHEN Xiayue MU Changkao LI Ronghua YE Yangfang SONG Weiwei SHI Ce LIU Lei WANG Huan WANG Chunlin collaborative innovation center for zhejiang marine high-efficiency and healthy aquaculture Ningbo UniversityNingbo 315211China Key Laboratory of Applied marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315211China

The swimming crab Portunus trituberculatus is an economically important marine crustacean *** isobaric tags for relative and absolute quantitation(iTRAQ)analysis were used to identify proteins that are differentially expressed during larval development of *** to elucidate the underlying *** comparison with the first zoea stage(Z1),3980 proteins were identified from 32789 peptides,which were matched with 115497 spectrums.A total of 241 proteins were screened with significantly differential expressions in all development *** 241 proteins are involved in various biological processes,such as cytoskeleton organization,protein synthesis,energy production and substance metabolism,physiological activities,and *** analyses of the 241 differentially expressed proteins led to the generation of four protein clusters based on the overall similarity in protein expression *** 54,70,36,and 45 proteins clustered in profiles 10(0,0,0,−1,0,0),15(0,1,0,1,0,1),18(0,1,2,2,1,0),and 19(0,1,2,3,4,5),*** development and exoskeleton renewal were important processes throughout the development *** addition,protein synthesis,degradation,and digestion actively occurred,especially at the Z4 *** results provide novel insights into the mechanisms underlying larval development of swimming crab and can assist in larval rearing.

关键词： Portunus trituberculatus iTRAQ proteomics larval development

Effect of Long-Term Low-Salinity Culture on the Survival, Growth, and Nutrient Composition of Mud Crab Scylla paramamosain

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Journal of Ocean University of China 2022年第1期21卷 179-185页

作者： JI Zilu LI Ronghua WANG Chunlin ZHANG Zhouyi ZHANG Weijia MU Changkao SONG Weiwei Key Laboratory of Applied marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315211China collaborative innovation center for zhejiang marine high-efficiency and healthy aquaculture Ningbo UniversityNingbo 315211China

This study investigated the effects of long-term low-salinity on the growth performance,proximate composition,amino acid and fatty acid profiles of Scylla *** salinity of the low-salinity-culture group(LC)and maintained-salinity-cul-ture group(MC)were set at 5 and 25 *** rearing for 30 d,the survival rate(SR)of the LC group was significantly lower,whereas the average daily molting frequency(ADMF)was significantly higher than those in MC group(Phigher than the MC group without significant *** contents of ash,crude lipid and crude pro-tein in the LC group were lower than those in the MC group,but no significant differences were found except for ash content.A total of 17 free amino acids and 23 fatty acids were identified from the muscle of *** total amino acids(TAA),essen-tial amino acids(EAA)and essential amino acid index(EAAI)had no significant difference between the two *** crabs from LC group had a significantly higher HUFA,including EPA,DHA,*** percentage of n-3 PUFA and the ratio of n-3/n-6 PUFA were significantly higher in the LC *** results indicated that *** rearing at low salinity had significantly de-creased SR;however,the low-salinity environment significantly promoted the accumulation of unsaturated fatty acids(UFAs),espe-cially the n-3 PUFA,which might be related to the osmoregulation mechanism.

关键词： Scylla paramamosain low salinity survival growth nutrient composition

Physiological and Molecular Analyses of Low-Salinity Stress Response in the Cuttlefish(Sepia pharaonis)Juveniles

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Journal of Ocean University of China 2022年第4期21卷 969-976页

作者： XIN Hongwei WU Kunlan YUAN Yimeng ZHAO Yun SONG Weiwei WANG Chunlin MU Changkao LI Ronghua Key Laboratory of Applied marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315832China collaborative innovation center for zhejiang marine high-efficiency efficiency and healthy aquaculture NingboUniversityNingbo 315832China

As a stenohaline species,the survival of Sepia pharaonis can be affected by salinity *** study aimed to explore the function of decreasing salinity on the survival of Sepia pharaonis,which can provide an advanced production guide on the culture of *** in the rainy *** was gradually decreased from 29 to 22 within 48 h to acclimate *** to a low-salinity *** ten days of breeding under low-salinity of 22,the death rate was *** this process,changes in tissue and cell structures in the larval liver,biochemical indicators,and osmoregulation-related gene expression were ***-terestingly,hepatocytes in the low-salinity group were irregular,had dissolved tissue inclusions,and contained vacuolized ***-fore,low salinity caused severe damages at a cellular level that can elevate the mortality rate.A gradual decline in salinity limited the full adaptation of *** indicators and osmoregulation-related gene expression changed *** instance,the trend of malondialdehyde(MAD)as a product of lipid peroxidation reflected the degree of damage to the body by free *** antioxidant system of *** could cope with oxidative stress caused by the change in salinity to a certain ***-regulation-related genes’expression also showed an optimistic result,that is,*** responded positively to the change in sali-nity by adjusting the expression of osmoregulation-related ***,the increase in mortality at day 10 also proved the weak adaptation capability of *** study indicated that *** can adapt to a low-salinity environment with a li-mited extent.

关键词： Sepia pharaonis low salinity physiological responses biochemical indicators osmoregulation-related genes

Drivers of coastal bacterioplankton community diversity and structure along a nutrient gradient in the East China Sea

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Journal of Oceanology and Limnology 2018年第2期36卷 329-340页

作者： HE Jiaying WANG Kai XIONG Jinbo GUO Annan ZHANG Demin FEI Yuejun YE Xiansen School of marine Sciences Ningbo University Ningbo 315211 China collaborative innovation center for zhejiang marine high-efficiency and healthy aquaculture Ningbo 315200 China marine Environmental Monitoring center of Ningbo State Oceanic Administration （SOA） Ningbo 315211 China

Anthropogenic nutrient discharge poses widespread threats to coastal ecosystems and has increased environmental gradients from coast to sea. Bacterioplankton play crucial roles in coastal biogeochemical cycling, and a variety of factors af fect bacterial community diversity and structure. We used 16 S r RNA gene pyrosequencing to investigate the spatial variation in bacterial community composition(BCC) across five sites on a coast-of fshore gradient in the East China Sea. Overall, bacterial alpha-diversity did not diff er across sites, except that richness and phylogenetic diversity were lower in the of fshore sites, and the highest alpha-diversity was found in the most landward site, with Chl-abeing the main factor. BCCs generally clustered into coastal and of fshore groups. Chl-a explained 12.3% of the variation in BCCs, more than that explained by either the physicochemical(5.7%) or spatial(8.5%) variables. Nutrients(particularly nitrate and phosphate), along with phytoplankton abundance, were more important than other physicochemical factors, co-explaining 20.0% of the variation in BCCs. Additionally, a series of discriminant families(primarily affiliated with Gammaproteobacteria and Alphaproteobacteria), whose relative abundances correlated with Chl-a, DIN, and phosphate concentrations, were identified, implying their potential to indicate phytoplankton blooms and nutrient enrichment in this marine ecosystem. This study provides insight into bacterioplankton response patterns along a coast-of fshore gradient, with phytoplankton abundance increasing in the of fshore sites. Time-series sampling across multiple transects should be performed to determine the seasonal and spatial patterns in bacterial diversity and community structure along this gradient.

关键词： bacterioplankton pyrosequencing nutrient gradient phytoplankton abundance dominant factor bioindicator