Phospholipid Compositions in Portunus trituberculatus Larvae at Different Developmental Stages

作     者：LU Zhibin SHI Ce LIU Lei MU Changkao YE Yangfang WANG Chunlin LU Zhibin;SHI Ce;LIU Lei;MU Changkao;YE Yangfang;WANG Chunlin

作者机构：Key Laboratory of Applied Marine BiotechnologyNingbo UniversityChinese Ministry of EducationNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy AquacultureNingbo 315211China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2022年第21卷第1期

页      面：152-162页

核心收录：

学科分类：090801[农学-水产养殖] 0908[农学-水产] 09[农学] 

基　　金：the National Natural Science Foundation of China(Nos.41673076,32073024) the Col-laborative Promotion Program of Zhejiang Province Ag-ricultural Technology of China(No.2020XTTGSC03) the China Agriculture Research System-CARS48 and K.C.Wong Magna Fund in Ningbo University are acknowle-dged 

主　　题：Portunus trituberculatus larval development phospholipid UHPLC-MS 

摘      要：Phospholipids are used to improve the growth and survival of Portunus trituberculatus,a widely cultured crab species in ***,only total phospholipids or several classes are applied in crab *** this study,we employed a targeted lipidomic method to investigate the comprehensive phospholipid composition in *** larvae and reveal the changing phospholipid profile over the larval *** showed that *** larvae contain 112 phospholipid species belonging to 10 phospholipid classes,in which phosphatidylcholine(PC)and phosphatidylethanolamine(PE)species are the most abundant,and PC,PE,phosphatidic acid,and phosphatidylserine(PS)are with high *** levels of all phospholipids significantly changed with larval development,which was highlighted by the downward parabolic changes in PE,phosphatidylglycerol,phosphatidylino-sitol,PS,lysophosphatidic acid,and sphingomyelin *** addition,nearly all phospholipid species were depleted at the M stage,which probably contributed to the mass mortality of crab *** findings on the composition and alterations of phospholipids in *** larvae provide novel perspectives for the targeted supplementation of phospholipids in crab *** work also highlights the use of targeted UHPLC-MS lipidomics in understanding the changes of phospholipids during crab development.