Dynamic metabolite alterations of Portunus trituberculatus during larval development

作     者：SHI Ce ZENG Tinglan LI Ronghua WANG Chunlin YE Yangfang MU Changkao 

作者机构：Key Laboratory of Applied Marine Biotechnology Ningbo University Chinese Ministry of Education Collaborative Innovation Center for Zhejiang Marine High-effi ciency and Healthy Aquaculture 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2019年第37卷第1期

页      面：361-372页

核心收录：

学科分类：07[理学] 

基　　金：Supported by the National Natural Science Foundation of China(No.41673076) the Major Agriculture Program of Ningbo(No.2017C110007) China Agriculture Research System-CARS48 the K.C.Wong Magna Fund in Ningbo University 

主　　题：Portunus trituberculatus larval development metabolite phenotype nuclear magnetic resonance(NMR) 

摘      要：A mass mortality often occurs from molting to the megalopa stage during the larval development of the swimming crab Portunus trituberculatus. Larvae with insuffcient nutrient accumulation during the zoeal stages are probably unable to develop into juvenile swimming crabs. However, the nutritional information such as the primary metabolites is scarce for P. trituberculatus larvae. The aim of this work is to obtain an insight into the metabolite traits of P. trituberculatus at early developmental stages. 1 H nuclear magnetic resonance spectroscopy coupled with multivariate data analysis was used to determine how the metabolite pro?les shift during larval development in P. trituberculatus. Our results show that the trend of total metabolites exhibited a rise from zoea 1 to zoea 3, followed by a drop from zoea 4 to megalopa and recovery during the ?rst juvenile stage. A large-scale depletion of total metabolites in the zoea 4 and megalopa stages suggests a deep depression of metabolic activity, which may be linked to the mass mortality from molting to the megalopa stage. These ?ndings provided essential metabolic information about the larval development of P. trituberculatus and important clues for understanding the nutritional requirements of P. trituberculatus in early developmental stages.