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Chinese Journal of Structural Chemistry 2013年第6期32卷 847-852页

作者：段李平吴宁波刘丛珊陶奕张雪强张浩冰 National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention Key Laboratory of Parasitology and Vector Biology at National Institute of Parasitic Diseases

The title compound 2-（2-methoxy-4-nitrophenylcarbamoyl）phenyl-4＇-fluorobenzoate （C21H15FN206, Mr = 410.35）, a fluorine-containing derivative of salicylamide, was conve- niently synthesized through two steps and crystallized in the orthorhombic space group P21/c with a = 7.6453（15）, b = 14.323（3）, c = 17.035（3） A, V= 1865.4（6） A3, Z = 4, Dc = 1.461 Mg/m^3, 2 = 0.71073 A, μ（MoKa） = 0.115 mm-1, F（000） = 848, R = 0.0705 and wR = 0.1834 for 3267 independent refections with 1 〉 2σ（I）. X-ray analysis reveals that the dihedral angles formed between the 2-methoxy-4-nitrobenzene and benzene ring, the benzene and 4-fluorobenzene, and the 2-methoxy-4-nitrobenzene and 4-fluorobenzene ring are 3.2（4）, 69.8（3） and 72.9（2）~, respectively. Bioassay shows that the title compound has anti-parasitic activity against hydatid protoscoleces.

关键词： 2-(2-methoxy-4-nitrophenyicarbamoyl)phenyl-4'-fluorobenzoate synthesis anti-parasitic crystal structure

Introducing nitazoxanide as a promising alternative treatment for symptomatic to metronidazole-resistant giardiasis in clinical isolates

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Asian Pacific Journal of Tropical Medicine 2016年第9期9卷 866-871页

作者： Tahereh Mikaeili Galeh Abdolhassan Kazemi Mahmoud Mahami-Oskouei Behzad Baradaran Adel Spotin Seddigheh Sarafraz Majid Karamat Immunology Research center Tabriz University of Medical Sciences Department of parasitology and Mycology Faculty of MedicineTabriz University of Medical Sciences East Azerbaijan Province Health center Tabriz Health Center

Objective:To identify the frequencies(F) of ferredoxin and nitroreductase mutations were identified on Iranian clinical isolates of Giardia lamblia in order to predict whether the nitazoxanide can be prescribed as suitable drug for symptomatic to metronidazoleresistant ***:Forty Giardia lamblia isolates as of 38 symptomatic and two metronidazole-resistant patients were collected from *** were extracted and amplified by targeting ferredoxin and Gl NR *** amplicons were directly sequenced to determine gene ***:The various amino acid substitutions(F:20%,Haplotype diversity:0.891,Tajima's D:-0.44013) were identified by analyzing ferredoxin gene in four symptomatic and two resistant ***,two haplotypes(F:5%,HD:0.345; Tajima's D:0.77815) characterized in metronidazole-resistant isolates of Gl NR,however,no point mutations was found in symptomatic ***:Non-synonymous mutations of ferredoxin oxidoreductase gene reduce translational regulatory protein's binding affinity which concludes reduction of ferredoxin expression and its *** leads to decrease in metronidazole drug delivery into the *** in these isolates may lead to their resistance to *** to low synonymous mutations of Gl NR demonstrates that nitazoxanide can be prescribed as promising alternative treatment for symptomatic to metronidazole-resistant giardiasis in Iranian clinical isolates.

关键词： Giardia lamblia Ferredoxin oxidoreductase Nitroreductase Nitazoxanide Metronidazole

Identification and characterization of the causative triatomine bugs of anaphylactic shock in Zhanjiang,China

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Infectious Diseases of Poverty 2018年第1期7卷 1325-1333页

作者： Ya-Lan Huang Da-Na Huang Wei-Hua Wu Fan Yang Xiao-Min Zhang Miao Wang Yi-Jun Tang Qian Zhang Li-Fei Peng Ren-Li Zhang Shenzhen center for Disease Control and Prevention Shenzhen 518055China Department of parasitology Guangdong Medical UniversityZhanjiang 524005China

Background:Two health concerns primarily related to triatomine bugs are transmission of Trypanosoma cruzi through infective feces,and allergic reactions induced by triatomine *** the Southwestern United States,reduviid bugs bites commonly cause insect *** South China,four cases of anaphylactic shock have been reported after this bite *** further classify the species of these bugs and confirm the sensitization of the triatomine saliva,we caught triatomine bugs from the region where the bites occurred and performed phylogenetic and immunohistochemical(IHC)***:Triatomine bugs were collected in Donghai Island of Zhanjiang City in South *** genomic DNA was extracted from three legs of the *** fragments of mitochondrial 16S rRNA,cytochrome c oxidase subunit I(COI)gene and nuclear ribosomal 18S and 28S rRNA genes were obtained by PCR and sequenced.A phylogenetic tree was constructed based on the sequence of 16S rRNA gene using a maximum likelihood method with MEGA 7.0 *** specific fragments and vertebrate COI genes were amplified from the fecal DNA to detect the infection of trypanosomes and analyze the blood feeding patterns,***-embedded sections were then prepared from adult triatomines and sent for IHC ***:We collected two adult triatomine bugs in Donghai *** and molecular analyses indicated that the triatomines were Triatoma *** fragments of *** or other trypanosomes were detected from the fecal *** gene segments of Homo sapiens and Mus musculus were successfully *** allergens which induced specific IgE antibodies in human serum were localized in the triatomine saliva by IHC ***:The two triatomine bugs from Donghai Island were *** had bitten humans and *** saliva should contain the allergens related to the allergic symptoms and even anaphylactic shock of exposed *** consi

关键词： Triatoma rubrofasciata Chagas disease Trypanosoma cruzi Anaphylactic shock

Phylogeography,genetic variability and structure of Acanthamoeba metapopulations in Iran inferred by 18S ribosomal RNA sequences:A systematic review and meta-analysis

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Asian Pacific Journal of Tropical Medicine 2017年第9期10卷 855-863页

作者： Adel Spotin Hamid Reza Moslemzadeh Mahmoud Mahami-Oskouei Ehsan Ahmadpour Maryam Niyyati Seyed Hossein Hejazi Fatemeh Memari Jafar Noori Immunology Research center Tabriz University of Medical SciencesTabrizIran Department of parasitology and Mycology Faculty of MedicineTabriz University of Medical SciencesTabrizIran Department of Medical parasitology and Mycology School of MedicineShahid Beheshti University of Medical SciencesTehranIran Department of parasitology and Mycology School of MedicineIsfahan University of Medical SciencesIsfahanIran

Objective: To verify phylogeography and genetic structure of Acanthamoeba populations among the Iranian clinical isolates and natural/artificial environments distributed in various regions of the ***: We searched electronic databases including Medline, Pub Med, Science Direct, Scopus and Google Scholar from 2005 to 2016. To explore the genetic variability of Acanthamoeba sp, 205 sequences were retrieved from keratitis patients, immunosuppressed cases and environmental sources as of various geographies of ***: T4 genotype was the predominant strain in Iran, and the rare genotypes belonged to T2, T3, T5(Acanthamoeba lenticulata), T6, T9, T11, T13 and T15(Acanthamoeba jacobsi).A total of 47 unique haplotypes of T4 were identified. A parsimonious network of the sequence haplotypes demonstrated star-like feature containing haplogroups IR6(34.1%) and IR7(31.2%) as the most common haplotypes. In accordance with the analysis of molecular variance, the high value of haplotype diversity(0.612–0.848) of Acanthamoeba T4 represented genetic variability within populations. Neutrality indices of the 18 S ribosomal RNA demonstrated negative values in all populations which represented a considerable divergence from neutrality. The majority of genetic diversity belonged to the infected contact lens and dust samples in immunodeficiency and ophthalmology wards, which indicated potential routes for exposure to a pathogenic Acanthamoeba sp. in at-risk individuals. A pairwise fixation index(FST) was from low to high values(0.024 33–0.418 92). The statistically FST points out that T4 is genetically differentiated between north-west, north-south and centralsouth metapopulations, but not differentiated between west-central, west-south, centralsouth, and north-central ***: An occurrence of IR6 and IR7 displays that possibly a gene flow of Acanthamoeba T4 occurred after the founder effect or bottleneck experience through ecological changes or host mobility

关键词： Acanthamoeba metapopulations Genetic variability Gene flow Molecular phylogeny Iran

Study on Leishmania infection in cats from Ahar,East Azerbaijan Province and North West Iran by parasitological,serological and molecular methods

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Asian Pacific Journal of Tropical Biomedicine 2015年第1期5卷 40-43页

作者： Taher Nemati Majid Khanmohammadi Ahad Bazmani Nasrin Mirsamadi Mohammad Hassan Kohansal Koshki Mehdi Mohebali Mohammad Fatollahzadeh Esmail Fallah Tabriz Infectious and Tropical Disease Research center Department of ParasitologyFaculty of MedicineTabriz University of Medical Sciences Department of parasitology Iran University of Medical Science Department of parasitology Central LaboratoryTabriz University of Medical Sciences Department of parasitology and Mycology Zanjan University of Medical Sciences Department of Medical parasitology and Mycology School of Public HealthTehran University of Medical Sciences

Objective:To study Leishmania infection in cats and its potential role in triuismission of the disease to human by parasitological,serological and molecular methods in Ahar District,East Azerbaijan ***:In this study,65 cats from different parts of Ahar Province were *** cats were anesthetized with chloroform and blood samples were taken from jugular vein and tested by direct agglutination *** and liver smear samples were prepared in order to microscopically examine these organs,and also cultured in Novy-MucNeal-Nicolle and Roswell Park Memorial Institute 1 641) ***,spleen tissue DNA was extracted to perform polymerase chain reaction ***:In direct agglutination test,4(6%) cats had a positive titer,while 14(22%) cats had a titer of 1:80 which was suspected for an infection and 47(729c) cats were *** results were negative and ill polymerase chain reaction no amplification was ***:Wc found no case of feline visceral *** needs more extensive studies by using a larger number of cats to firmly establish leishmaniasis in this area.

关键词： Cat Ahar Leishmania

Giardia duodenalis infection among rural communities in Yemen:A community-based assessment of the prevalence and associated risk factors

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Asian Pacific Journal of Tropical Medicine 2017年第10期10卷 987-995页

作者： Hesham M.Al-Mekhlafi Endemic and Tropical Diseases Unit Medical Research CenterJazan UniversityJazanSaudi Arabia Department of parasitology Faculty of MedicineUniversity of Malaya50603 Kuala LumpurMalaysia Department of parasitology Faculty of Medicine and Health SciencesSana'a UniversitySana'aYemen

Objective: To investigate the prevalence and risk factors of Giardia infection among rural communities among rural communities in ***: In this cross-sectional study, a total of 605 stool samples were collected and screened for the presence of Giardia duodenalis(***) cysts and/or trophozoites by using three different diagnostic methods: direct smear, formalin-ether sedimentation,and trichrome staining.A pre-tested questionnaire was used to collect information on the demographic, socioeconomic, behavioural and environmental characteristics of the ***: Overall, 28.1%(170/605) of the participants were infected by *** prevalence was significantly higher among male participants compared to female(P = 0.034); however, it was not significant among different age groups(P > 0.05).Univariate and multivariate analyses identified four variables as the significant key risk factors of Giardia infection among the sampled *** are, in addition to being of the male gender, using unsafe water sources for drinking water, not washing hands after defecation, presence of other family members infected with Giardia, and close contact with domestic ***: The study reveals that Giardia infection is still prevalent among rural communities in *** provision of clean and safe drinking water, proper sanitation,and health education regarding personal hygiene practices, particularly handwashing, as well as identifying and treating infected family members is imperative and these interventions should be considered in a strategy to control intestinal parasites among these communities in order to curtail the transmission and morbidity caused by ***.

关键词： Giardia duodenalis Prevalence Risk factors Yemen

Molecular characterization of Echinococcus granulosus in paraffin-embedded human tissues from Southwest Iran

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Asian Pacific Journal of Tropical Medicine 2019年第11期12卷 507-511页

作者： Elham Yousefi Abdollah Rafiei Iran Rashidi Shahram Khademvatan Masoud Foroutan Department of Medical parasitology Ahvaz Jundishapur University of Medical SciencesAhvazIran Department of Pathology Ahvaz Jundishapur University of Medical SciencesAhvazIran Cellular and Molecular Research center and Department of Medical parasitology Urmia University of Medical SciencesUrmiaIran Cellular and Molecular Research center Ahvaz Jundishapur University of Medical SciencesIran Department of parasitology Faculty of Medical SciencesTarbiat Modares UniversityTehranIran

Objective:To investigate Echinococcus(E.)granulosus genotypes as the causative agents of hydatidosis in humans in the southwest of Iran(Khuzestan province).Methods:In this study,isolates of 80 archived human paraffin embedded hydatid cysts were collected from pathology laboratories in Ahvaz city,Khuzestan *** was extracted and examined by nested-PCR of ribosomal DNA(rDNA)internal transcribed spacer 1(ITS1),and *** addition,the sequences of fragments of genes coding for Cox space1 and NADH dehydrogenase 1(ND1)were also ***:Of the 80 paraffin samples,44(55.0%)were from the liver,27(33.8%)from the lung,and the rest from other *** amplified hydatid genomic DNA showed that the cysts were *** *** results of PCR-RFLP and sequencing analysis revealed the presence of G1 genotype(sheep strain)in all human ***,no camel strain(G6)was detected among all samples in the regions ***:The molecular findings indicate that the predominant genotype involved in *** transmission in southwest of Iran is the common sheep strain(G1),which occurs in human *** results may have important implications for hydatid disease control in the studied areas.

关键词： Echinococcus granulosus Human Genotype Iran

Identification and Analysis of Genes Present in Leptospira interrogans serovar lai but Absent in L. biflexa serovar monvalerio

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 832-839页

作者： Ping HE, Xiang-Yan ZHANG, Xiao-Kui GUO*, Bao-Yu HU, Xiao-Tian HUANG, Yang YANG, and Guo-Ping ZHAO1,2* Department of Microbiology and parasitology, Shanghai Second Medical University, Shanghai 200025, China 1 Research center of Biotechnology, Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China 2Chinese National Human Genome center at Shanghai, Zhangjiang High Tech Park, Shanghai 201203, China Department of Microbiology and parasitology Shanghai Second Medical UniversityShanghai 200025 China Research center of Biotechnology Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Chinese National Human Genome center at ShanghaiZhangjiang High Tech Park Shanghai 201203 China

Genes present in virulent bacterial strains but absent in avirulent close relatives can be of great biologic and clinical interest. This project aimed to identify strain specific DNA sequences of Leptospira interrogans serovar lai, which is absent in the saprophytic L. biflexa serovar monvalerio, via suppression subtractive hybridization with the former as the tester while the latter as the driver. The mixture of PCR amplified DNA fragments from two subtractive hybridization experiments were cloned into pMD18-T vector and the positive clones were identified by dot blotting against the chromosome DNA of the two strains individually. After DNA sequencing and analysis, the distribution of these genomic fragment sequences in a panel of pathogenic and nonpathogenic leptospires was investigated employing dot blot analysis. Among the 188 positive clones randomly chosen, 24 contained the tester strain specific genomic regions, of which, 5 were non-coding fragments while the others contained 23 distinct protein coding sequences. Besides 9 genes encoding functional proteins, 12 genes encode unknown proteins and the rest two genes encode proteins with recognizable domain structures, one for a putative leucine-rich repeats (LRR) family protein while the other as an outer-membrane protein. Our experiment results indicated that suppression subtractive hybridiza- tion is effective for screening specific DNA sequences between two leptospiral strains, and some of these sequences might be responsible for virulence determination. Further analysis of these DNA sequences will provide important information on the pathogenesis of Leptospira.

关键词： Leptospira interrogans serovar lai suppression subtractive hybridization (SSH) specific genes

Global prevalence,mortality,and main risk factors for COVID-19 associated pneumocystosis:A systematic review and meta-analysis

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Asian Pacific Journal of Tropical Medicine 2022年第10期15卷 431-441页

作者： Hossein Khodadadi Ehsan Ahmadpour Sanam Nami Rasoul Mohammadi Hanieh Hosseini Mahsa Behravan Hamid Morovati Department of parasitology and Mycology School of MedicineShiraz University of Medical SciencesShirazIran Drug Applied Research center Tabriz University of Medical SciencesTabrizIran Department of Medical Mycology and parasitology School of MedicineTabriz University of Medical SciencesTabrizIran Department of Medical parasitology and Mycology School of MedicineIsfahan University of Medical SciencesIsfahanIran Immunology Research center Tabriz University of Medical SciencesTabrizIran Student Research Committee Shiraz University of Medical SciencesShirazIran

Objective:Pneumocystis pneumonia(PcP)is a life-threatening infection caused by the opportunistic fungi Pneumocystis *** emergence of the COVID-19 pandemic forced the focus of attention of health policymakers on these two infections due to their clinical and paraclinical similarities,which cause diagnostic *** study was undertaken to evaluate and estimate the global prevalence and main leading risk factors of coronavirus-associated pneumocystosis(CAP).Methods:We searched related databases between December 2019 and May 2022 for studies reporting ***-analysis was performed using StatsDirect software(version 2.7.9)according to the DerSimonian and Laird method applying the random-effects *** evaluated heterogeneity using theχ2-based Q statistic(significant for P75%indicative of“notable”heterogeneity).Moreover,an odds ratio(OR)analysis was performed for eligible ***:Our meta-analysis included eight studies with 923 patients hospitalized with COVID-19;among them,92 were PcP *** overall pooled prevalence of CAP was estimated at 11.5%.The mortality among CAP patients was lower than that of non-PcP patients(OR 1.93;95%CI 0.86-4.31).Long-term corticosteroid therapy(OR 28.22;95%CI 0.54-1480.84)was the most predisposing factor for PcP among COVID-19 patients,followed by pulmonary diseases(OR 1.46;95%CI 0.43-4.98),kidney diseases(OR 1.26;95%CI 0.21-7.49),and acute respiratory destruction syndrome(OR 1.22;95%CI 0.05-29.28).Conclusions:The prevalence of PcP among the COVID-19 population is almost similar to the pre-COVID ***,PcP-related mortality was decreased by the emergence of the COVID-19 *** with COVID-19 are more susceptible to PcP than *** respiratory distress syndrome,kidney diseases,pulmonary diseases,and long-term corticosteroid therapy increased the risk of PcP;however,transplantation and malignancy decreased the risk for PcP among COVID-19 *** retrospective,cas

关键词： Coronavirus disease 19 COVID-19 SARS-CoV-2 Pneumocystis pneumonia(PcP) COVID-associated infections Prevalence Odds ratio Risk factors

Protective effect of DNA vaccine with the gene encoding 55kDa antigen fragment against Pneumocystis carinii in mice

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Asian Pacific Journal of Tropical Medicine 2011年第5期4卷 353-356页

作者： Yi-nong Duan Liang-heng Yi Jin-ling Chen Dan-dan Zhu Jian-xin Wang Jin-rong Feng Yong-wei Qin Ying Zhu Department of parasitology and Microbiology School of MedicineNantong University Kunshan Red Cross Society Laboratory Medicine center Affiliated Hospital of Nantong University

Objective:To evaluate the protective effect of DNA vaccine with the gene encoding 55kDa antigen fragment of Pneumocystis carinii(***) against *** in ***:The fragment of the antigen within p55(p55-582) was *** recombinant plasmid was constructed based on the eukaryotic expression vector pcDNA3.1(+).BALB/c mice were used as experimental models to examine the immunogenicity of pcDNA3.1(+)-*** and RTPCR were used to evaluate the role of this kind of DNA ***:The results of western blot indicated that the recombinant DNA[pcDNA3.1(+)-p55-582]could be expressed correctly and had antigenicity in transfected COS-7 *** and RT-PCR showed that pcDNA3.1(+)- p55-582 elicited antibody production,stimulated lymphocyte proliferation and provided partial protection by reducing the *** ***:The data demonstrate that pcDNA3.1(+)-p55-582 might be potent vaccination that can afford the partial protection for the immunized animals.

关键词： Pneumocystis carinii DNA vaccine p55