Study on Leishmania infection in cats from Ahar,East Azerbaijan Province and North West Iran by parasitological,serological and molecular methods

作     者：Taher Nemati Majid Khanmohammadi Ahad Bazmani Nasrin Mirsamadi Mohammad Hassan Kohansal Koshki Mehdi Mohebali Mohammad Fatollahzadeh Esmail Fallah 

作者机构：Tabriz Infectious and Tropical Disease Research CenterDepartment of ParasitologyFaculty of MedicineTabriz University of Medical Sciences Department of ParasitologyIran University of Medical Science Department of ParasitologyCentral LaboratoryTabriz University of Medical Sciences Department of Parasitology and MycologyZanjan University of Medical Sciences Department of Medical Parasitology and MycologySchool of Public HealthTehran University of Medical Sciences 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2015年第5卷第1期

页      面：40-43页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 10[医学] 

基　　金：Supported by Infectious Disease and Tropical Research Center of Tabriz University of Medical Sciences(Project No.92-02) 

主　　题：Cat Ahar Leishmania 

摘      要：Objective:To study Leishmania infection in cats and its potential role in triuismission of the disease to human by parasitological,serological and molecular methods in Ahar District,East Azerbaijan Province.Methods:In this study,65 cats from different parts of Ahar Province were trapped.The cats were anesthetized with chloroform and blood samples were taken from jugular vein and tested by direct agglutination lest.Spleen and liver smear samples were prepared in order to microscopically examine these organs,and also cultured in Novy-MucNeal-Nicolle and Roswell Park Memorial Institute 1 641) media.Finally,spleen tissue DNA was extracted to perform polymerase chain reaction analysis.Results:In direct agglutination test,4(6%) cats had a positive titer,while 14(22%) cats had a titer of 1:80 which was suspected for an infection and 47(729c) cats were negative.Culture results were negative and ill polymerase chain reaction no amplification was observed.Conclusions:Wc found no case of feline visceral leishmaniasis.It needs more extensive studies by using a larger number of cats to firmly establish leishmaniasis in this area.