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Identification of serum immunoglobulin M(IgM)of crucian carp(Carassius auratus gibelio)and immune response to cyprinid herpesvirus 2 infection

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Aquaculture and Fisheries 2024年第2期9卷 172-180页

作者： Shuran Wang Haotian Wu Longlong Wang Pinhua Wang Liqun Lu Yousheng Jiang Ye Zhang Dan Xu National Pathogen Collection center for Aquatic Animals Shanghai Ocean UniversityShanghai201306China Key Laboratory of Aquatic Genetic Resources of the Ministry of Agriculture Shanghai Ocean UniversityShanghai201306China National Demonstration center for Experimental Fisheries science Education Shanghai Ocean UniversityShanghai201306China

Crucian carp(Carassius auratus gibelio),an extensively cultivated freshwater fish,was one of the model species for the study of fish *** antibodies were advantageous molecular tools for studying teleost immune ***,polyclonal antibodies reacting with immunoglobulins(Ig)were used successfully in studies of the teleost *** the present study,we produced polyclonal antibody against CH2 domains of crucian carp IgM,and measured the in vivo dynamics of IgM mRNA caused by CyHV-2 *** recombinant protein IgM with relative molecular weight about 53 KD was correctly expressed in prokaryotic *** specificity of the polyclonal antibody was evaluated by Western blotting and results revealed that the antibody not only specifically recognized crucian carp serum but also cross-reacted with grass carp *** RT-PCR analysis demonstrated the expression of IgM mRNA changed significantly after CyHV-2 *** expression of IgM in the kidney increased and reached a maximum at 6 h post-infection(hpi),while dropped to a low level at 5 days post-infection(dpi).In conclusion,the expression of IgM was significantly upregulated in the kidney of crucian carp infected with CyHV-2,indicating that IgM played a potential role in systemic immunity against viral *** antibody against crucian carp IgM had certain clinical relevance,which might provide insight into the early stage of virus infection and prevention of the disease.

关键词： Immunoglobulin M Crucian carp Polyclonal antibody Cyprinid herpesvirus 2 Immunoprotection

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Application of the Conditional Nonlinear Local Lyapunov Exponent to Second-Kind Predictability

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Advances in Atmospheric sciences 2024年第9期41卷 1769-1786页

作者： Ming ZHANG Ruiqiang DING Quanjia ZHONG Jianping LI Deyu LU State Key Laboratory of Earth Surface Processes and Resource Ecology(ESPRE)/Key Laboratory of Environmental Change and Natural Disasters of Chinese Ministry of EducationBeijing Normal UniversityBeijing 100875China Department of ocean science Hong Kong University of Science and TechnologyHong KongChina College of oceanic and Atmospheric sciences/Frontiers science center for Deep ocean Multispheres and Earth System(DOMES)/Key Laboratory of Physical oceanography Ministry of Education/Academy of the Future Ocean/Center for Ocean Carbon NeutralityOcean University of ChinaQingdao 266100China Laoshan Laboratory Qingdao 266237China State Key Laboratory of Numerical Modeling for Atmospheric sciences and Geophysical Fluid Dynamics(LASG) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China College of Earth science University of Chinese Academy of SciencesBeijing 100049China

In order to quantify the influence of external forcings on the predictability limit using observational data,the author introduced an algorithm of the conditional nonlinear local Lyapunov exponent(CNLLE)*** effectiveness of this algorithm is validated and compared with the nonlinear local Lyapunov exponent(NLLE)and signal-to-noise ratio methods using a coupled Lorenz *** results show that the CNLLE method is able to capture the slow error growth constrained by external forcings,therefore,it can quantify the predictability limit induced by the external *** this basis,a preliminary attempt was made to apply this method to measure the influence of ENSO on the predictability limit for both atmospheric and oceanic variable *** spatial distribution of the predictability limit induced by ENSO is similar to that arising from the initial conditions calculated by the NLLE *** similarity supports ENSO as the major predictable signal for weather and climate *** addition,a ratio of predictability limit(RPL)calculated by the CNLLE method to that calculated by the NLLE method was *** RPL larger than 1 indicates that the external forcings can significantly benefit the long-term predictability *** instance,ENSO can effectively extend the predictability limit arising from the initial conditions of sea surface temperature over the tropical Indian ocean by approximately four months,as well as the predictability limit of sea level pressure over the eastern and western Pacific ***,the impact of ENSO on the geopotential height predictability limit is primarily confined to the troposphere.

关键词： conditional nonlinear local Lyapunov exponent second-kind predictability coupled Lorenz model ENSO

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Production and characterization of monoclonal antibodies against grass carp CD4-1 and CD4-2

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Aquaculture and Fisheries 2024年第2期9卷 154-163页

作者： Bangjie Wang Yuting Qin Zhaosheng Sun Xiaozhen Zhu Zhao Jia Kangyong Chen Danjie Liu Junya Wang Jun Zou Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China International Research center for Marine Biosciences at Shanghai ocean University Ministry of Science and TechnologyShanghai201306China National Demonstration center for Experimental Fisheries science Education Shanghai Ocean UniversityShanghai201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266071China

CD4 T helper cells are an important group of cells in the immune system of vertebrates and express CD4 receptor on the cell *** mammals,the CD4 receptor is encoded by a single copy gene,whilst in fish,two copies of cd4 genes,namely cd4-1 and cd4-2,are *** this study,the ectodomains of grass carp(Ctenopharyngodon idella,Ci)CD4-1 and CD4-2 were expressed in the *** cells and used to generate monoclonal antibodies in *** blotting,confocal microscopy and flow cytometry were performed to characterize the monoclonal *** has been shown that the CiCD4-1 and CiCD4-2 monoclonal antibodies had good specificity to react with the recombinant ectodomains of CiCD4-1 and CiCD4-2 expressed in the CHO-S cells and the native CD4 molecules of grass *** CiCD4-1 monoclonal antibody did not recognize CiCD4-2 and verse *** addition,the CD4-1 and CD4-2 monoclonal antibodies specifically recognized the CD4-1 and CD4-2 receptors expressed in the HEK293 cells and native molecules of fish ***,the percentages of lymphocytes in immune tissues of healthy fish were analyzed by flow *** was found that 17.6%of lymphocytes were CD4-1^(+)cells and 22.5%were CD4-2^(+)in the head *** the spleen,13.1%of lymphocytes were CD4-1^(+)and 18.6%were CD4-2^(+)while 7.3%of blood lymphocytes were CD4-1^(+)cells and 8.8%were CD4-2^(+)*** availability of the CD4-1 and CD4-2 monoclonal antibodies provides antibody-based tools for further elucidation of the functions of CD4 T cells in grass carp.

关键词： Grass carp CD4 Monoclonal antibodies Flow cytometry Confocal microscopy

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Recent Progress in Optical Sensors Based on MXenes Quantum Dots and MXenes Nanosheets

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Journal of Analysis and Testing 2024年第1期8卷 95-113页

作者： Chunxiao Lin Xinhong Song Weilan Ye Ting Liu Mingcong Rong Li Niu Guangzhou Key Laboratory of Sensing Materials and Devices/center for Advanced Analytical science/School of Chemistry and Chemical Engineering Guangzhou UniversityGuangzhou 510006China College of ocean science and Engineering Shanghai Maritime UniversityShanghai 201306China

In recent years,MXenes,as a new promising two-dimensional material,have received extensive attentions in the fields of environmental remediation,catalysts,sensors,and energy *** broaden the applications of MXenes in sensing,MXenes nanosheets(MNSs) and MXenes quantum dots(MQDs) are prepared by physical etching or chemical *** are monolayered or multilayered MXenes nanosheets within several nanometers in height,and can serve as the perfect reaction substrate and fluorescence *** are spherical,fluorescent MXenes QDs within several nanometers in size,which possess favorable stability and photo *** the distinct features such as high conductivity,favorable hydrophilicity,biocompatibility,abundant surface functional groups,and easy functionalization,MNSs and MQDs show potential applications in the fields of anti-counterfeiting,environmental monitoring,biosensing and *** review focuses on the research of MNS s/MQDs-based optical sensors,including colorimetric sensors,fluorescence sensors,electrochemiluminescence sensors,surface plasmon resonance sensors,and surface-enhanced Raman scattering ***,the current challenges and prospects concerning MNSs/MQDs-based optical sensing techniques are discussed.

关键词： MXenes quantum dots Fluorescence Colorimetry Electrochemiluminescence Surface plasmon resonance

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Exposure to microplastics induces lower survival,oxidative stress,disordered microbiota and altered metabolism in the intestines of grass carp(Ctenopharyngodon idella)

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Aquaculture and Fisheries 2024年第5期9卷 785-794页

作者： Xuewen Jia Yang Liu Yan He Hongyan Yu Yuting Liu Yubang Shen Xiaoyan Xu Jiale Li Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs Shanghai Ocean UniversityShanghai201306China National Demonstration center for Experimental Fisheries science Education Shanghai Ocean UniversityShanghai201306China Shanghai Engineering Research center of Aquaculture Shanghai Ocean UniversityShanghai201306China

There are major gaps in illustrating the risk to freshwater fish that are exposed to environmental microplastics(MPs),even though MPs in freshwater environments has drawn much *** explore the potentially negative effects of microplastics on freshwater fish,grass carp(averaging 9.215±0.035g)were exposed to microplastics(32-40μm in diameter)at two environmental concentrations(100 and 1000μg/L)for 21 days,and evaluated for physio-biochemical performance,gut microbiome,and metabolome *** exposure,microplastics were detected in the intestine and within basal nuclei and goblet cells *** enzyme activities(superoxide dismutase and catalase)also significantly decreased within the intestines(P<0.05).Through 16S rRNA gene sequencing analysis,a decrease in diversity and richness was observed in the MPs-H and MPs-L *** abundance of opportunistic pathogens increased and beneficial bacteria decreased in both MP *** example,Acinetobacter and Bosea proportions were higher in the MPs-L group,while Bacteroides and Shewanella increased in the MPs-H ***,abundance of Lactobacillus decreased both in the MPs-H and MPs-L *** analysis revealed primary bile acid biosynthesis metabolism upregulated in the MPs-L and purine metabolism downregulated in the MPs-H ***,an integration analysis of the gut microbiome and metabolomics identified significant associations between several bacteria taxa and metabolites related to intestinal *** together,these results highlighted the negative effects on intestinal morphology,oxidative stress,and microbiome and emphasized the relationship between microbiota and metabolites in response to microplastics,which could provide comprehensive insights into the potential risks of MPs on freshwater fish intestines.

关键词： Microplastics Oxidative stress Metabolomics Microbiome Grass carp Intestine

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Characterisation of IL-21 and IL-21Rαin grass carp:IL-21-producing cells are upregulated during Flavobacterium columnare infection

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Aquaculture and Fisheries 2024年第2期9卷 126-135页

作者： Jiawen Xu Junya Wang Qingyu Min Wei Wang Yuting Qin Lina Lei Qian Gao Jun Zou Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China International Research center for Marine Biosciences at Shanghai ocean University Ministry of Science and TechnologyShanghai201306China National Demonstration center for Experimental Fisheries science Education Shanghai Ocean UniversityShanghai201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266200China

Interleukin-21(IL-21)is produced mainly by activated CD4^(+)T cells and promotes the proliferation,survival and differentiation of immune *** study characterised a high affinity binding receptor of IL-21(IL-21 Rα)from grass carp(Ctenopharyngodon idella,Ci)and analyzed the expression of CiIL-21 and CiIL-21 Rα.It has been shown that they were modulated by PAMPs and during infection with bacterial and viral *** CiIL-21 protein produced in bacteria was active in inducing the expression of genes involved in Th responses.A monoclonal CiIL-21 antibody was generated against the rCiIL-21 and showed good reactive specificity with the rCiIL-21 proteins expressed in *** cells,and HEK293 T cells and the native protein from PHA-stimulated kidney *** addition,the IL-21-producing cells detected by the CiIL-21 monoclonal antibody were found to be increased to in the kidney,spleen and hindgut after infection with Flavobacterium *** data indicate that IL-21 is involved in the regulation of immune response to bacterial and viral infection.

关键词： IL-21 IL-21Rα Gene expression IL-21-Producing cell Bacterial and viral infection Fish

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Using Natural Radionuclides to Trace Sources of Suspended Particles in the Lower Reaches of the Yellow River

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Journal of ocean University of China 2024年第1期23卷 99-108页

作者： ZHANG Han BURNETT CWilliam ZHANG Xiaojie ZHAO Shibin YANG Disong NAN Haiming YU Zhigang XU Bochao Frontiers science center for Deep ocean Multispheres and Earth System and Key Laboratory of Marine Chemistry Theory and TechnologyMinistry of EducationOcean University of ChinaQingdao 266100China Laboratory for Marine Ecology and Environmental science Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China College of Chemistry and Chemical Engineering Ocean University of ChinaQingdao 266100China Department of Earth Ocean and Atmospheric ScienceFlorida State UniversityTallahasseeFL 32306USA North China Sea Environmental Monitoring center State Oceanic AdministrationKey Laboratory of Ecological Prewarning and Protection of Bohai SeaMNRQingdao 266033China Shandong Provincial Key Laboratory of Marine Ecological Environment and Disaster Prevention and Mitigation Qingdao 266033China

Natural radionuclides are powerful tools for understanding the sources and fate of suspended particulate matter(SPM).Particulate matter with different particle sizes behaves differently with respect to adsorption and *** analyzed the activi-ties and distribution characteristics of multiple natural radionuclides(238U,226Ra,40K,228Ra,7Be and 210Pbex)on size-fractionated SPM at the Lijin Hydrographic Station(Huanghe or Yellow River)every month over a one-year *** showed that medium silt(16–32µm)was the main *** expected,the activity of each radionuclide decreased with an increase of particle *** examined the sources of SPM with different particle sizes using activity ratios of 226Ra/238U,228Ra/226Ra,40K/238U and 7Be/210Pbex,and concluded that SPM with different particle sizes originated from different *** results indicate that fine SPM(32µm)was mainly derived from resuspension of riverbed *** high runoff periods,the concentration of SPM increased significantly,and the pro-portion of fine particles originating upstream *** occurring radioactive isotopes,especially on size-fractionated par-ticles,are therefore seen as useful tracers to understand the sources and behaviors of riverine particles transported from land to sea.

关键词： the Yellow River suspended particulate matter size-fractions radionuclides sediment sources water elutriator

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RNA-seq analysis of a zebrafish caudal fin cell line in response to infection with spring viraemia of carp virus

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Aquaculture and Fisheries 2024年第2期9卷 181-192页

作者： Wenji Huang Ning Ji Xin Zhao Jiahong Guo Jianhua Feng Kangyong Chen Yaxin Wu Junya Wang Jun Zou Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China International Research center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai201306China National Demonstration center for Experimental Fisheries science Education Shanghai Ocean UniversityShanghai201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266071China

A cell line,termed ZFIN,was established from the caudal fin of zebrafish and was shown to be susceptible to spring viremia of carp virus(SVCV).The ZFIN cells are epithelial like cells and have a moderate plasmid transfection efficiency of 13.9%.Using an RNA-seq approach,differentially expressed genes(DEGs)regulated by SVCV were *** of SVCV gave rise to 3931 DEGs and up-regulated DEGs were mostly enriched into the biological regulation and cellular processes,among which pathways for the type I interferon signaling and the response to exogenous dsRNA were the top two GO *** KEGG signaling pathways including TLR signaling pathway,RLR receptor signaling pathway,cytosolic DNA-sensing pathway,NLR signaling pathway,cytokine-cytokine receptor interaction and ferroptosis were significantly *** genes including ifnφ1,isg15 and mx were significantly *** addition,key DEGs involved in autophagy were *** results indicate that the ZFIN cell line provides a useful in vitro tool for study on the gene functions and cellular responses to viral infection in fish.

关键词： Caudal fin cells SVCV RNA-seq DEGs Innate antiviral response

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Volatile compounds,synergistic effects,precursors and impact factors for odor profiles in Eriocheir sinensis

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Aquaculture and Fisheries 2024年第5期9卷 721-730页

作者： Qi Lu Lizhi Yu Xueqian Guo Xichang Wang College of Food science and Technology Shanghai Ocean UniversityShanghai201306China Shanghai Engineering Research center of Aquaculture Shanghai Ocean UniversityShanghai201306China Shanghai Engineering Research center of Aquatic-Product Processing and Preservation Shanghai201306China

Flavor,especially odor,is one of the most concerning food qualities for producers and *** odor of *** is a vital quality indicator and highly variable in species,diets,regions and harvest *** differences could diminish consumer acceptance and reduce the economic returns to the *** now,168 volatile compounds have been identified in ***,of which 42 are shared to five edible parts,and the hepatopancreas has 22 specific *** 30 aroma-active compounds may interact to form odor *** compounds are mainly derived from Phosphatidylethanolamine(PE)and free fatty acids(FFA)by a series of biochemical reactions such as thermal degradation,secondary degradation,molecular rearrangement,and Maillard *** odor of crab has a close relationship with *** harvested,the crab also produces odors during processing and storage,which could be controlled by *** work provides information that could support the development of innovative aquaculture strategies to satisfy the needs of the consumer market.

关键词： Volatiles Precursors Impact factors Eriocheir sinensis

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Double enzyme mimetic activities of multifunctional Ag nanoparticle-decorated Co_(3)V_(2)O_(8)hollow hexagonal prismatic pencils for application in colorimetric sensors and disinfection

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Nano Materials science 2024年第2期6卷 244-255页

作者： Ying Gao Peng Ju Yu Zhang Yuxin Zhang Xiaofan Zhai Jizhou Duan Baorong Hou Key Laboratory of Marine Environmental Corrosion and Bio-fouling Institute of OceanologyChinese Academy of SciencesQingdao266071PR China Key Laboratory of Marine Eco-Environmental science and Technology Marine Bioresource and Environment Research CenterFirst Institute of OceanographyMinistry of Natural ResourcesQingdao266061PR China University of Chinese Academy of sciences Beijing100049PR China Open Studio for Marine Corrosion and Protection Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266071PR China center for ocean mega-science Chinese Academy of SciencesQingdao266071PR China College of Materials science and Engineering Chongqing UniversityChongqing400044China

Since the catalytic activity of most nanozymes is still far lower than the corresponding natural enzymes,there is urgent need to discover novel highly efficient enzyme-like *** this work,Co_(3)V_(2)O_(8)with hollow hexagonal prismatic pencil structures were prepared as novel artificial enzyme *** were then decorated by photo-depositing Ag nanoparticles(Ag NPs)on the surface to further improve its catalytic *** Ag NPs decorated Co_(3)V_(2)O_(8)(ACVPs)showed both excellent oxidase-and peroxidase-like catalytic *** can oxidize the colorless 3,3’,5,5’-tetramethylbenzidine rapidly to induce a blue *** enhanced enzyme mimetic activities can be attributed to the surface plasma resonance(SPR)effect of Ag NPs as well as the synergistic catalytic effect between Ag NPs and Co_(3)V_(2)O_(8),accelerating electron transfer and promoting the catalytic *** were applied in constructing a colorimetric sensor,validating the occurrence of the Fenton reaction,and disinfection,presenting favorable catalytic *** enzyme-like catalytic mechanism was studied,indicating the chief role of⋅O_(2)-radicals in the catalytic *** work not only discovers a novel functional material with double enzyme mimetic activity but also provides a new insight into exploiting artificial enzyme mimics with highly efficient catalytic ability.

关键词： Co_(3)V_(2)O_(8) Ag NPs Enzyme mimetic Colorimetric sensor Disinfection

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