Characterisation of IL-21 and IL-21Rαin grass carp:IL-21-producing cells are upregulated during Flavobacterium columnare infection
作者机构：Key Laboratory of Exploration and Utilization of Aquatic Genetic ResourcesMinistry of EducationShanghai Ocean UniversityShanghai201306China International Research Center for Marine Biosciences at Shanghai Ocean UniversityMinistry of Science and TechnologyShanghai201306China National Demonstration Center for Experimental Fisheries Science EducationShanghai Ocean UniversityShanghai201306China Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdao266200China
出 版 物：《Aquaculture and Fisheries》 (渔业学报（英文）)
年 卷 期：2024年第9卷第2期
页 面：126-135页
核心收录：
基 金：funded by the National Natural Science Foundation of China(Grant number:32030112) National Key R&D Program of China(Grant number:2018YFD0900302)
主 题：IL-21 IL-21Rα Gene expression IL-21-Producing cell Bacterial and viral infection Fish
摘 要：Interleukin-21(IL-21)is produced mainly by activated CD4^(+)T cells and promotes the proliferation,survival and differentiation of immune *** study characterised a high affinity binding receptor of IL-21(IL-21 Rα)from grass carp(Ctenopharyngodon idella,Ci)and analyzed the expression of CiIL-21 and CiIL-21 Rα.It has been shown that they were modulated by PAMPs and during infection with bacterial and viral *** CiIL-21 protein produced in bacteria was active in inducing the expression of genes involved in Th responses.A monoclonal CiIL-21 antibody was generated against the rCiIL-21 and showed good reactive specificity with the rCiIL-21 proteins expressed in *** cells,and HEK293 T cells and the native protein from PHA-stimulated kidney *** addition,the IL-21-producing cells detected by the CiIL-21 monoclonal antibody were found to be increased to in the kidney,spleen and hindgut after infection with Flavobacterium *** data indicate that IL-21 is involved in the regulation of immune response to bacterial and viral infection.
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