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Acta Metallurgica Sinica(English Letters) 2021年第3期34卷 435-443页

作者： Wei Yang Xiaoxian Pang Zhao Xue Jinhao Ye Haosen Fan Ting Shu Wenzhi Zheng Shengzhou Chen School of Chemistry and Chemical Engineering Guangzhou UniversityGuangzhou 510006China Patent Examination Cooperation(Henan)Center of the Patent Office Zhengzhou 450046China Key Laboratory of Fuel cell Technology of Guangdong Province Guangzhou 510640China

Ni3[Fe(CN)6]2 nano-cubic precursors were prepared by chemical coprecipitation at room temperature with nickel acetate and potas sium ferricyanide as raw *** corresponding NiFe2O4-NiO@C composites with excellent crystallization were prepared by two-stage oxidation at low *** microstructure and electrochemical behavior of the materials showed that the Prussian blue analog was transformed into metal oxide while the carbon coating was maintained in the twostage oxidation at low *** existence of the carbon coating reduces the charge transfer impedance to 31.5 Ω.At the current density of 500 mA/g,the reversible capacity of 632.7 mAh/g is maintained after 500 *** the same time,carbon cladding can also enhance the role of pseudocapacitance in the *** the scanning rate of 0.1 mV/s,the pseudocapacitance account for 54.4% of the total discharge capacity,which is significantly higher than that of uncoated materials.

关键词： Lithium-ion battery Anode materials Metal oxide Prussian blue

Long noncoding RNA HOXA11-AS promotes gastric cancer cell proliferation and invasion via SRSF1 and functions as a biomarker in gastric cancer

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World Journal of Gastroenterology 2019年第22期25卷 2763-2775页

作者： Yun Liu Yu-Mei Zhang Feng-Bo Ma Su-Rong Pan Bao-Zhen Liu Department of Operating room Binzhou People's HospitalBinzhou 256610Shandong ProvinceChina Department of Return Visit Binzhou People's HospitalBinzhou 256610Shandong ProvinceChina Department of Gastroenterology Binzhou People's HospitalBinzhou 256610Shandong ProvinceChina

BACKGROUND Gastric cancer (GC) is the fourth most frequent malignancy all over the world. The diagnosis of GC is challenging and the prognosis of GC is very unfavorable. Accumulating evidence reveals that serum long noncoding RNAs (lncRNAs) can function as biomarkers in various types of cancers, including GC. AIM To explore the level and molecular mechanism of the lncRNA HOXA11-AS in GC and the diagnostic and prognostic significance of serum HOXA11-AS in GC. METHODS HOXA11-AS levels in GC tissue, cell lines, and serum samples were measured. The correlation between HOXA11-AS expression and clinicopathological characteristics was analyzed. The role of HOXA11-AS in the diagnosis and prognosis of GC was evaluated. cell function assays were performed for exploration of the roles of HOXA11-AS in GC cells. Moreover, Western blot was performed to explore the target regulated by HOXA11-AS in GC cells. RESULTS Up-regulation of HOXA11-AS was found in GC tissues, cell lines, and serum samples. In GC patients, decreased serum HOXA11-AS levels were negatively related with tumor size, TNM stage, and lymph node metastasis. The area under the receiver operating characteristic curve of serum HOXA11-AS in the diagnosis of GC was 0.924 (95%CI: 0.881-0.967;sensitivity, 0.787;specificity 0.978). Results of the Kaplan-Meier survival curves suggested the GC patients with a lower HOXA11-AS level having a better overall survival rate. HOXA11-AS promoted GC cell proliferation and invasion. SRSF1 may be the target regulated by HOXA11-AS in GC cells. CONCLUSION HOXA11-AS promotes GC cell proliferation and invasion via SRSF1 and may function as a promising marker in GC.

关键词： Long noncoding RNA HOXA11-AS SRSF1 Gastric cancer Biomarker

Detection of CD2 expression in chicken hematogenic embryo yolk sac lymphoid cells prior to thymus genesis

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Progress in Natural Science:Materials International 2008年第12期18卷 1589-1594页

作者： Dongyu Zhou~a Jigui Wang~b Weiquan Liu~b Rongxiu Liu~c Yuehu Pei~a a College of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China b College of Biological Sciences,China Agricultural University,Beijing 100094,China c Liaoning Tianhe Biotechnology Co.Ltd,room 1203,100-1 Dongbinhe Road,Shenyang District,Shenyang 110016,China [a]College of Traditional Chinese Materia Medica Shenyang Pharmaceutical University Shenyang 110016 China [b]College of Biological Sciences China Agricultural University Beijing 100094 China [c]Liaoning Tianhe Biotechnology Co. Ltd Room 1203 100-1 Dongbinhe Road Shenyang District Shenyang 110016 China

Lymphoid mononuclear cells from chick embryos at stage 16 were collected prior to fetal liver and thymus genesis to study the dif- ferentiation and function of the hematogenic yolk sac and to detect whether CD2 occurs on the surface of lymphoid mononuclear cells. The phenotype and functional activity of the cell surface protein E receptor and the ultrastructure of embryonic E~+ cells were compared with those of mature T *** results indicate 99.36% homology between the E receptors of embryonic lymphocytes and mature T *** similarities,including molecular distribution,motivation,the ability to form an erythrocyte rosette,the structure of the receptor-ligand complex,and the conformation of the signal channel,were detected between embryonic lymphocytes and mature CD2-expressing T *** results indicate that CD2 is already expressed prior to fetal liver and thymus genesis and that its expression is not dependent on the thymic microenvironment.

关键词： CD2 Chicken embryo Hematogenic yolk sac

Effects of electro-acupuncture on Noxa and caspase-3 expression in hippocampal CA1 region of a vascular dementia rat model

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Neural Regeneration Research 2008年第8期3卷 826-831页

作者： Yanzhen Zhu Qiang Wu Ling Lin Department of Integrative Medicine Fujian University of Traditional Chinese Medicine Fuzhou 350108 Fujian Province. China Department of Acupuncture and Moxibustion Fujian University of Traditional Chinese Medicine Fuzhou 350108 Fujian Province. China The room for Confocal Laser Scanning Microscope Fujian Medical University Fuzhou 350004 Fujian Province China

BACKGROUND：Noxa, a pro-apoptotic member of the Bcl-2 protein family, has been shown to induce the mitochondrial pathway of apoptosis and to mediate hypoxic cell death in a rat model of cerebral ischemia. This suggests that Noxa could participate in apoptosis during vascular dementia （VD）. OBJECTIVE： To detect Noxa and caspase-3 expression after electro-acupuncture in VD rats to further validate the mechanism of electro-acupuncture-induced effects in the treatment of VD. DESIGN, TIME AND SETTING： A randomized, controlled study was performed at the Center for the Neurobiology of Fujian Medical University between January 2006 and March 2007. MATERIALS： A total of forty adult, male, Sprague Dawley rats were included in this study. The following equipment was used： confocal laser scanning microscope （Sp5, Leica, Germany）, water maze （Bejing Suntendy Science and Technology Co., Ltd., China）, and SDZ-II electronic acupuncture treatment instruments （Suzhou Medical Appliance Factory, China）. METHODS： Thirty-eight rats with sufficient learning and memory abilities were selected by Morris water maze criteria. Twelve rats received sham-surgery; the remaining 26 rats were used to establish a VD model by bilateral occlusion of the common carotid arteries. The rats that survived the occlusion procedure were randomly assigned into an electro-acupuncture group （n = 11） and a VD model group （n = 12）. MAIN OUTCOME MEASURES： Neuropathological changes were observed with hematoxylin-eosin staining of the hippocampus and expression of Noxa and caspase-3 in the hippocampal CA1 region was analyzed by confocal laser scanning microscope following immunofluorescence staining. RESULTS： Expressions of Noxa and caspase-3 in the electro-acupuncture group and sham-operated group were less than in the VD model group （P 〈 0.01）. Electro-acupuncture reduced the amount of apoptotic neurons in hippocampal CA1 area of rats with VD. The average latency in the Morris water maze test was significantly short

关键词： apoptosis caspase-3 electro-acupuncture Noxa vascular dementia

Relationship of ultrasonic elastography parameters of cervical cancer with the cancer cell growth and angiogenesis in the lesion tissue

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Journal of Hainan Medical University 2017年第20期23卷 97-100页

作者： Bing-Yan Lai B-Ultrasound room Shaanxi Provincial Hospital of Traditional Chinese MedicineXi'an CityShaanxi Province710002

Objective: To study the relationship of ultrasonic elastography parameters of cervical cancer with the cancer cell growth and angiogenesis in the lesion tissue. Methods: A total of 110 patients who were diagnosed with cervical cancer in the hospital between December 2015 and January 2017 were collected as the observation group, 80 patients who received hysteroscopic cervical polyp resection in the hospital during the same period were collected as the control group. The levels of cervical ultrasonic elastography parameters in the two groups were detected, and fluorescence quantitative PCR was used to determine the expression of proliferation genes, apoptosis genes and angiogenesis-related genes in the lesion tissue. Pearson test was used to evaluate the correlation between cervical ultrasonic elastography parameters and the tumor malignancy indexes. Results: Elastic image press release index and strain ratio of local lesion of observation group were significantly higher than those of control group;GBP1 mRNA expression in lesion tissue of observation group was lower than that in lesion tissue of control group while Prdx4, STAT3 and Sp2 mRNA expression were higher than those in lesion tissue of control group;Survivin, FasL and Bcl-2 mRNA expression in lesion tissue of observation group were higher than those in lesion tissue of control group while Fas and Bax mRNA expression were lower than those in lesion tissue of control group;VEGF, MMP-9, COX-2 and HIF-1 mRNA in lesion tissue of observation group were significantly higher than those in lesion tissue of control group. Conclusion: Elastic image press release index and strain ratio of cervical tissue of patients with cervical cancer are higher, and the specific increase is directly correlated with the tumor malignancy.

关键词： Cervical cancer Ultrasonic elastography parameter Proliferation gene Apoptosis gene Angiogenesis-related gene

Expression of trefoil factors and TWIST1 in colorectal cancer and their correlation with metastatic potential and prognosis

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World Journal of Gastroenterology 2017年第1期23卷 110-120页

作者： Akram Yusup Bailikezi Huji Cheng Fang Fei Wang Tuerxunjiang Dadihan Hai-Jiang Wang Halmurat Upur Department of Gastrointestinal Surgery and Record room Affiliated Tumor HospitalXin Jiang Medical University Department of Gastrointestinal Surgery Xi Jing Digestive Disease HospitalFourth Military Medical University Department of Human Anatomy of Preclinical College Xinjiang Medical University Central Laboratory Xinjiang Medical University

AIM To detect the expression of trefoil factors (TFFs) and TWIST1 in colorectal cancer (CRC) and analyze their correlation with metastasis and survival. METHODS This study examined the expression of TFF1, TFF3 and TWIST1 in a total of 75 tumor samples, 47 matched normal samples (15 cm from the lesion margin), 30 metastatic lymph nodes, and 10 liver metastatic cancer samples from patients with CRC. The relationship was then analyzed between the protein expression and different clinical records. TFF1, TFF3, TWIST1, E-cadherin, vimentin and beta-catenin mRNA and protein expression levels were measured in colon cancer cell lines with different metastatic potentials (HIEC, HT29, SW620, and LoVo cells), and the correlation of the expression levels with epithelial-mesenchymal transition (EMT) was discussed. RESULTS It was found that 66.7% (50/75), 78.7% (59/75) and 54.7% (41/75) of tumor tissue samples exhibited positive staining for TFF1, TFF3 and TWIST1 and so did 27.3% (13/47), 100% (47/47) and 17% (8/47) of adjacent normal colorectal tissues. Compared with adjacent normal tissues, significant differences were found in the expression of all three proteins in different cancerous tissues (P cell lines was higher than that in HIEC (a normal human intestinal epithelial cell line)(P < 0.05), and the expression intensity demonstrated a tendency to rise with increased metastatic potential both at the protein and mRNA levels. However, TFF1 expression demonstrated the opposite tendency. It was also observed that the expression of E-cadherin and beta-catenin tended to decrease while that of vimentin, TWIST1 and Snail tended to rise with the increase in metastatic potential. CONCLUSION The exp

关键词： Colorectal cancer Trefoil factors TWIST1 Survival Metastasis

Mesenchymal stem cell-based therapy for ischemic stroke

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Chinese Neurosurgical Journal 2016年第4期2卷 233-238页

作者： Johnathon D.Anderson Missy T.Pham Zelenia Contreras Madeline Hoon Kyle D.Fink Henrik J.Johansson Julien Rossignol Department of Internal Medicine Stem Cell Program University of California Davis Davis USAInstitute for Regenerative Cures University of California Davis Medical Center 2921 Stockton Blvd Room 1300 Sacramento CA 95817 USA Department of Internal Medicine Stem Cell Program University of California Davis Davis USA Department of Oncology-Pathology Karolinska InstituteCancer Proteomics Davis USA College of Medicine Central Michigan University Mt PleasantUSA

Ischemic stroke represents a major,worldwide health burden with increasing *** affected by ischemic strokes currently have few clinically approved treatment options *** currently approved treatments for ischemic stroke have narrow therapeutic windows,severely limiting the number of patients able to be *** stem cells represent a promising novel treatment for ischemic *** studies have demonstrated that mesenchymal stem cells functionally improve outcomes in rodent models of ischemic *** studies have also shown that exosomes secreted by mesenchymal stem cells mediate much of this *** the present review,we summarize the current literature on the use of mesenchymal stem cells to treat ischemic *** studies investigating the mechanisms underlying mesenchymal stem cells tissue healing effects are warranted and would be of benefit to the field.

关键词： Mesenchymal stem cells Exosomes Ischemic stroke

Development of PDMS-based Microfluidic Device for cell-based Assays

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高等学校化学学报 2004年第Z1期25卷 4-6页

作者： LI Chenuk-Wing YANG Jun TZANG Chi-Hung YANG Meng-Su Applied Research Centre for Genomic Technology and Department of Biology and ChemistryCity University of Hong Kong 83 Tat Chee Avenue Kowloon Hong Kong China Applied Research Centre for Genomic Technology and Department of Biology and ChemistryCity University of Hong Kong 83 Tat Chee Avenue Kowloon Hong Kong China Applied Research Centre for Genomic Technology and Department of Biology and ChemistryCity University of Hong Kong 83 Tat Chee Avenue Kowloon Hong Kong China Applied Research Centre for Genomic Technology and Department of Biology and ChemistryCity University of Hong Kong 83 Tat Chee Avenue Kowloon Hong Kong China

In a single step photolithography, muhi-level microfluidic device is fabricated by printing novel architectures on a film photomasks. The whole fabrication process is executed by classical PCB technology without the need to access clean room facilities. Different levels of protruding features on PCB master are produced by exposing a photomask with specifically arranged "windows and rims" architectures, followed by chemical wet etching. Poly(dimethylsiloxane)(PDMS) is then molded against the positive relief master to generate microfluidic device featured with multi-level sandbag structure and peripheral microchannels. This sandbag structure is an analog to traditional dam or weir for particle entrapment. The microstructure does not collapse when subjected to applied pressure, which is suitable for operation on elastic PDMS *** immunocytochemcial staining assays were performed in the microdevice to demonstrate the applicability of the sandbag structure for cellular analysis. This simplified microfabrication process employs low-cost materials and minimal specialized equipment and can reproducibly produce mask lines with about 20 μm in width, which is sufficient for most microfluidic applications.

关键词： Microfluidic device PDMS cell-based assays

MicroRNA signatures in liver diseases

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World Journal of Gastroenterology 2009年第14期15卷 1665-1672页

作者： Xian-Ming Chen Department of Medical Microbiology andImmunology Creighton University Medical Center Criss ⅡRoom 529 2500 California Plaza Omaha NE 68178United States

MicroRNAs (miRNAs) are an emerging class of highly conserved non-coding small RNAs that regulate gene expression at the post-transcriptional level. It is now clear that miRNAs can potentially regulate every aspect of cellular activity, including differentiation and development, metabolism, proliferation, apoptotic cell death, viral infection and tumorigenesis. Recent studies provide clear evidence that miRNAs are abundant in the liver and modulate a diverse spectrum of liver functions. Deregulation of miRNA expression may be a key pathogenetic factor in many liver diseases including viral hepatitis, hepatocellular cancer and polycystic liver diseases. A clearer understanding of the mechanisms involved in miRNA deregulation will offer new diagnostic and therapeutic strategies to treat liver diseases. Moreover, better understanding of miRNA regulation and identification of tissue-specific miRNA targets employing transgenic/knockout models and/or modulating oligonucleotides will improve our knowledge of liver physiology and diseases.

关键词： MicroRNAs Non-coding RNAs Liver Tumorigenesis Gene regulation

Studies on Biological Characteristics of Rabbit Oral Mucosal Epithelial cells

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Chinese Journal of Biomedical Engineering(English Edition) 2010年第2期19卷 85-92页

作者： WANG Wei CHEN Jian-su PANG Hong-wei LI Xiao-xia LI Yan Research room of Ophthalmology Medical College Key Laboratory for Regenerative MedicineMinistry of Education Jinan University Guangzhou 510632China

Objective:The biological characteristics of rabbit oral mucosal epithelial cells(OMECs) were studied and the suitable procedure for the culture of OMECs was ***:Cultured OMECs by different surface disinfection,by different Dispase Ⅱ preparation,in different medium,in different calciumion concentration of K-SFM ***:Microbial contamination of cell culture could significantly reduce with 2% iodine solution used for sterilization of oral cavity before cell *** OMECs digested with Dispase Ⅱin K-SFM could attach to the culture flask quickly,the OMECs digested with Dispase Ⅱ in PBS rare attached to the culture *** proliferations of OMECs cultured in 0 or 0.09 mmol/L calcium concentrations of K-SFM medium showed not statistically *** OMECs grew fast with serum,but it was easy to differentiate into fibroblast-like ***:OMECs could grow well and had the typical oral mucosal epithelial cell morphology by the procedure that described in this experiments.

关键词： cell culture oral mucosa serum-free culture calcium biological characteristics