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Isolation and culture of human primary osteoblasts:Comparing the effects of differences in method details on osteoblast characteristics

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Genes & Diseases 2024年第2期11卷 546-549页

作者： Fei Chen Yan Zhang Pai Peng Xiao-Ting Huang Zi-Han Qiu Bao-Cheng Liu Tie-Lin Yang Bo Yang Yan Guo Key Laboratory of biomedical Information engineering of Ministry of Education Biomedical Informatics & Genomics CenterSchool of Life Science and TechnologyXi'an Jiaotong UniversityXi'anShaanxi 710049China department of orthopaedics Shannxi Provincial People's Hospital (The Third Affiliated Hospital of Xi'an Jiaotong University)Xi'anShaanxi 710068China

Osteoblasts are essential in the maintenance of human bone homeostasis.1 The abnormal formation and impaired differentiation ability of osteoblasts are pivotal factors leading to bone-related diseases,suggesting that an indepth study of osteoblasts can provide novel treatment strategies for these diseases.2 Human primary osteoblasts are precious experimental models in bone-related research.3 However,the isolation and culture of human primary osteoblasts still have some difficulties and ***,we refined a method to isolate and culture human primary osteoblasts by taking cancellous bone through a bone tissue sampler and pre-digesting it with pancreatin,which makes the process easier,cheaper,and more ***,we compared the effects of the subtle changes in isolation and culture methods on the phenotype of human primary osteoblasts and examined the differences in morphology,proliferation,and differentiation between micro-explants cultured osteoblasts(MECOBs)and explants cultured osteoblasts(EC-OBs).

关键词： impaired culture details

Recent progress in functional metal-organic frameworks for bio-medical application

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Regenerative Biomaterials 2024年第2期3卷 37-54页

作者： Wenwen Chai Xiaochen Chen Jing Liu Liyan Zhang Chunyu Liu Li Li John Robert Honiball Haobo Pan Xu Cui Deping Wang School of Materials Science and engineering Tongji UniversityShanghai 201804China Shenzhen Institute of Advanced Technology Chinese Academy of SciencesShenzhen 518055China department of orthopaedics&Traumatology School of Clinical MedicineLi Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina

Metal-organic frameworks(MOFs)have a high specific surface area,adjustable pores and can be used to obtain functional porous materials with diverse and well-ordered structures through coordination and self-assembly,which has intrigued wide interest in a broad range of *** the arena of biomedical engineering,the functionalized modification of MOFs has produced drug carriers with excellent dispersion and functionalities such as target delivery and response release,with promising applications in bio-detection,disease therapy,tissue healing,and other *** review summa-rizes the present state of research on the functionalization of MOFs by physical binding or chemical cross-linking of small *** *** hvdrogels and evaluates the role and approach of MOFs functionalization in boosting the reactivity of *** this basis,research on the application of functionalized MOFs composites in biomedical engineering fields such as drug delivery,tissue repair,disease treatment,bio-detection and imaging is surveyed,and the development trend and application prospects of functionalized MOFs as an important new class of biomedical materials in the biomedical field are anticipated,which may provide some inspiration and reference for further development of MOF for bio-medical applications.

关键词： metal-organic frameworks porous functionalization tissue repair disease treatment

Delivering Microrobots in the Musculoskeletal System

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Nano-Micro Letters 2024年第11期16卷 585-617页

作者： Mumin Cao Renwang Sheng Yimin Sun Ying Cao Hao Wang Ming Zhang Yunmeng Pu Yucheng Gao Yuanwei Zhang Panpan Lu Gaojun Teng Qianqian Wang Yunfeng Rui department of orthopaedics Zhongda HospitalSchool of MedicineSoutheast UniversityNanjingJiangsuPeople’s Republic of China School of Medicine Southeast UniversityNanjing 210009People’s Republic of China Orthopaedic Trauma Institute(OTI) Southeast UniversityNanjing 210009People’s Republic of China Jiangsu Key Laboratory for Design and Manufacture of Micro‑Nano biomedical Instruments School of Mechanical EngineeringSoutheast UniversityNanjing 210009People’s Republic of China Center of Interventional Radiology and Vascular Surgery Department of RadiologyZhongda HospitalSchool of MedicineSoutheast UniversityNanjing 210009People’s Republic of China

Disorders of the musculoskeletal system are the major contributors to the global burden of disease and current treatments show limited *** often suffer chronic pain and might eventually have to undergo end-stage ***,future treatments should focus on early detection and intervention of regional *** have been gradually used in organisms due to their advantages of intelligent,precise and minimally invasive targeted *** the combination of control and imaging systems,microrobots with good biosafety can be delivered to the desired area for *** the musculoskeletal system,microrobots are mainly utilized to transport stem cells/drugs or to remove hazardous substances from the *** to traditional biomaterial and tissue engineering strategies,active motion improves the efficiency and penetration of local targeting of cells/*** review discusses the frontier applications of microrobotic systems in different tissues of the musculoskeletal *** summarize the challenges and barriers that hinder clinical translation by evaluating the characteristics of different microrobots and finally point out the future direction of microrobots in the musculoskeletal system.

关键词： Microrobot Musculoskeletal system Targeted delivery Microrobotic systems Magnetic actuation

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Bone Research 2024年第1期12卷 122-136页

作者： Nan-Yu Zou Ran Liu Mei Huang Yu-Rui Jiao Jie Wei Yangzi Jiang Wen-Zhen He Min Huang Yi-Li Xu Ling Liu Yu-Chen Sun Mi Yang Qi Guo Yan Huang Tian Su Ye Xiao Wei-Shan Wang Chao Zeng Guang-Hua Lei Xiang-Hang Luo Chang-Jun Li department of Endocrinology Endocrinology Research CenterXiangya Hospital of Central South UniversityChangshaHunan 410008China department of orthopaedics Xiangya Hospital of Central South UniversityChangshaHunan 410008China department of Epidemiology and Health Statistics Xiangya School of Public HealthCentral South UniversityChangshaChina Hunan Key Laboratory of Joint Degeneration and Injury ChangshaHunan 410008China Key Laboratory of Aging-related Bone and Joint Diseases Prevention and Treatment Ministry of EducationXiangya HospitalCentral South UniversityChangshaChina School of biomedical Sciences Institute for Tissue Engineering and Regenerative MedicineFaculty of MedicineThe Chinese University of Hong KongHong Kong SARChina department of orthopaedics The First Affiliated Hospital of Shihezi UniversityShiheziXinjiangChina National clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China

Skeletal stem/progenitor cell(SSPC)senescence is a major cause of decreased bone regenerative potential with aging,but the causes of SSPC senescence remain *** this study,we revealed that macrophages in calluses secrete prosenescent factors,including grancalcin(GCA),during aging,which triggers SSPC senescence and impairs fracture *** injection of human rGCA in young mice induced SSPC senescence and delayed fracture *** deletion of Gca in monocytes/macrophages was sufficient to rejuvenate fracture repair in aged mice and alleviate SSPC ***,GCA binds to the plexin-B2 receptor and activates Arg2-mediated mitochondrial dysfunction,resulting in cellular *** of Plxnb2 in SSPCs impaired fracture *** of GCA-neutralizing antibody enhanced fracture healing in aged ***,our study revealed that senescent macrophages within calluses secrete GCA to trigger SSPC secondary senescence,and GCA neutralization represents a promising therapy for nonunion or delayed union in elderly individuals.

关键词： healing union senescence

Electrospun PCL/gelatin/arbutin nanofiber membranes as potent reactive oxygen species scavengers to accelerate cutaneous wound healing

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Regenerative Biomaterials 2024年第3期3卷 43-55页

作者： Mindong Du Shuhan Liu Nihan Lan Ruiming Liang Shengde Liang Maoqiang Lan Disen Feng Li Zheng Qingjun Wei Ke Ma department of orthopaedics Trauma and Hand Surgery The First Affliated Hospital of Guangxi Medical UniversityGuangxi Medical UniversityNanning 530521China Guangxi engineering Center in biomedical Materials for Tissue and Organ Regeneration The First Affliated Hospital of Guangxi Medical UniversityGuangxi Medicar UniversityNanning530021China Guangxi Collaborative Innovation Center for Biomedicine The First Affliated Hospital of Guangxi Medical UniversityGuangxi Medical UniversityNanning 530021China department of Plastic&Cosmetic Surgery.The First Affliated Hospital of Guangxi Medical University Guangxi Medical UniversityNanning 530021China Pharmaceutical College Guangxi Medical UniversityNanning530021China

The presence of excessive reactive oxygen species(Ros)at a skin wound site is an important factor affecting wound *** scavenging,which regulates the ROS microenvironment,is essential for wound *** this study,we used novel electrospun PCL/gelatin/arbutin(PCL/G/A)nanofibrous membranes as wound dressings,with PCL/gelatin(PCL/G)as the backbone,and plantderived arbutin(hydroquinone-β-D-glucopyranoside,ARB)as an effective antioxidant that scavenges ROos and inhibits bacterial infection in *** loading of ARB increased the mechanical strength of the nanofibres,with a water vapour transmission rate of more than 2500g/(m^(2)×24h),and the water contact angle decreased,indicating that hydrophilicity and air permeability were significantly *** release and degradation experiments showed that the nanofibre membrane controlled the drug release and exhibited favourable *** experiments showed that the PCL/G/A nanofibre membranes were biocompatible,and DPPH and ABTS+radical scavenging experiments indicated that PCL/G/A could effectively scavenge ROS to reflect the antioxidant *** addition,haemostasis experiments showed that PCL/G/A had good haemostatic effects in vitro and in *** vivo animal wound closure and histological staining experiments demonstrated that PCL/G/A increased collagen deposition and remodelled epithelial tissue regeneration while showing good in vivo biocompatibility and *** conclusion,we successfully prepared a multifunctional wound dressing,PCL/G/A,for skin wound healing and investigated its potential role in wound healing,which is beneficial for the clinical translational application of phytomedicines.

关键词： arbutin nanofiber membranes wound dressing reactive oxygen species

Regulation of specific abnormal calcium signals in the hippocampal CA1 and primary cortex M1 alleviates the progression of temporal lobe epilepsy

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Neural Regeneration Research 2024年第2期19卷 425-433页

作者： Feng Chen Xi Dong Zhenhuan Wang Tongrui Wu Liangpeng Wei Yuanyuan Li Kai Zhang Zengguang Ma Chao Tian Jing Li Jingyu Zhao Wei Zhang Aili Liu Hui Shen Laboratory of Neurobiology School of Biomedical EngineeringTianjin Medical UniversityTianjinChina Institute for Translational Neuroscience the Second Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Institute for Translational Brain Research Fudan UniversityShanghaiChina department of Radiology the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjingJiangsu ProvinceChina Innovative Institute of Chinese Medicine and Pharmacy Shandong University of Traditional Chinese MedicineJinanChina department of Anesthesiology Tianjin Medical University General HospitalTianjinChina department of orthopaedics Tianjin Medical University General HospitalTianjinChina Tianjin Eye Hospital Tianjin Eye InstituteTianjin Key Lab of Ophthalmology and Visual ScienceTianjinChina Laboratory of Neurobiology School of Basic Medical SciencesTianjin Medical UniversityTianjinChina

Temporal lobe epilepsy is a multifactorial neurological dysfunction syndrome that is refractory,resistant to antiepileptic drugs,and has a high recurrence *** pathogenesis of temporal lobe epilepsy is complex and is not fully *** calcium dynamics have been implicated in temporal lobe ***,the effect of fluctuating calcium activity in CA1 pyramidal neurons on temporal lobe epilepsy is unknown,and no longitudinal studies have investigated calcium activity in pyramidal neurons in the hippocampal CA1 and primary motor cortex M1 of freely moving *** this study,we used a multichannel fiber photometry system to continuously record calcium signals in CA1 and M1 during the temporal lobe epilepsy *** found that calcium signals varied according to the grade of temporal lobe epilepsy *** particular,cortical spreading depression,which has recently been frequently used to represent the continuously and substantially increased calcium signals,was found to correspond to complex and severe behavioral characteristics of temporal lobe epilepsy ranging from gradeⅡto gradeⅤ.However,vigorous calcium oscillations and highly synchronized calcium signals in CA1 and M1 were strongly related to convulsive motor *** inhibition of pyramidal neurons in CA1 significantly attenuated the amplitudes of the calcium signals corresponding to gradeⅠ*** addition,the latency of cortical spreading depression was prolonged,and the above-mentioned abnormal calcium signals in CA1 and M1 were also significantly ***,it was possible to rescue the altered intracellular calcium *** simultaneous analysis of calcium signals and epileptic behaviors,we found that the progression of temporal lobe epilepsy was alleviated when specific calcium signals were reduced,and that the end-point behaviors of temporal lobe epilepsy were *** results indicate that the calcium dynamic between CA1 and M1 may reflect sp

关键词： Ca^(2+) calcium signals chemogenetic methods hippocampus primary motor cortex pyramidal neurons temporal lobe epilepsy

Axial load-share ratio testing to assess the healing of open tibial fractures treated with the Taylor Spatial Frame

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biomedical engineering Communications 2024年第3期3卷 23-30页

作者： Zhao Liu Jin-Wei Liu Qi-Jun Zhao Hong-Zhou Li Ning-Ning Zhang Hong-Liang Zhang Yu Gou Xun Sun Tao Zhang Wei-Guo Xu department of orthopaedics Tianjin HospitalTianjin UniversityTianjin 301617China clinical College of Orthopedics Tianjin Medical UniversityTianjin 301617China department of orthopaedics Sinopharm Northern HospitalBaotou 014010China

Background:The Taylor Spatial Frame(TSF)has gained popularity among orthopedic surgeons for treating open ***,a key challenge is the timely and safe removal of the *** study assessed the efficacy and safety of axial load-share ratio(ALSR)testing to evaluate callus healing strength after TSF treatment of open tibial ***:A retrospective case-control study was conducted,analyzing 180 adult patients with open tibial fractures treated at Tianjin Hospital’s Orthopedic Limb Correction Unit between August 2019 and August *** patients underwent TSF external fixation surgery,and were divided into two groups based on ALSR *** I(92 patients)underwent ALSR testing,with frame removal if the test value fell below 5%.Traditional methods were used for fixator removal guidance in Group II(88 patients).clinical outcomes,including fixation duration,complications after fixator removal,and Johner-Wruhs functional scores,were compared between the two ***:The groups showed no statistically significant differences(P>0.05)in sex,age,injury side,body mass index,surgery timing,or fracture *** I had a significantly shorter fixation duration(25.85±5.57 weeks)compared to Group II(31.82±6.98 weeks)(P0.05).Conclusion:Regular postoperative ALSR testing could safely and effectively guide TSF removal following open tibial fracture *** method significantly reduced fixation duration compared to traditional guidance methods while maintaining efficacy and safety.

关键词： tibial fractures external fixators fracture healing axial load

Biomimetic natural biomaterials for tissue engineering and regenerative medicine:new biosynthesis methods,recent advances,and emerging applications

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Military Medical Research 2024年第1期11卷 50-79页

作者： Shuai Liu Jiang-Ming Yu Yan-Chang Gan Xiao-Zhong Qiu Zhe-Chen Gao Huan Wang Shi-Xuan Chen Yuan Xiong Guo-Hui Liu Si-En Lin Alec McCarthy Johnson V.John Dai-Xu Wei Hong-Hao Hou Guangdong Provincial Key Laboratory of Construction and Detection in Tissue engineering the Fifth Affiliated HospitalSchool of Basic Medical ScienceSouthern Medical UniversityGuangzhou 510900China department of Orthopedics Tongren HospitalShanghai Jiao Tong UniversityShanghai 200336China The Eighth Affiliated Hospital Sun Yat-Sen UniversityShenzhen 518033GuangdongChina engineering Research Center of clinical Functional Materials and Diagnosis&Treatment Devices of Zhejiang Province Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325011ZhejiangChina department of Orthopedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China department of orthopaedics and Traumatology Faculty of Medicinethe Chinese University of Hong KongHong Kong SAR 999077China department of Functional Materials Terasaki Institute for Biomedical InnovationLos AngelesCA 90064USA Mary&Dick Holland Regenerative Medicine Program College of MedicineUniversity of Nebraska Medical CenterOmahaNE 68130USA Zigong Affiliated Hospital of Southwest Medical University Zigong Psychiatric Research CenterZigong Institute of Brain ScienceZigong 643002SichuanChina Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of EducationSchool of MedicineDepartment of Life Sciences and MedicineNorthwest UniversityXi’an 710127China

Biomimetic materials have emerged as attractive and competitive alternatives for tissue engineering(TE)and regenerative *** contrast to conventional biomaterials or synthetic materials,biomimetic scaffolds based on natural biomaterial can offer cells a broad spectrum of biochemical and biophysical cues that mimic the in vivo extracellular matrix(ECM).Additionally,such materials have mechanical adaptability,micro-structure interconnectivity,and inherent bioactivity,making them ideal for the design of living implants for specific applications in TE and regenerative *** paper provides an overview for recent progress of biomimetic natural biomaterials(BNBMs),including advances in their preparation,functionality,potential applications and future *** highlight recent advances in the fabrication of BNBMs and outline general strategies for functionalizing and tailoring the BNBMs with various biological and physicochemical characteristics of native ***,we offer an overview of recent key advances in the functionalization and applications of versatile BNBMs for TE ***,we conclude by offering our perspective on open challenges and future developments in this rapidly-evolving field.

关键词： Biomimic Scaffold Biosynthesis Natural biomaterial Tissue engineering

机械敏感蛋白PC1调控破骨细胞及骨吸收的作用机制

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Science Bulletin 2024年第12期69卷 1964-1979页

作者：黄梅周静璇李潇骁刘冉姜洋子陈开璇焦玉睿尹欣刘玲孙宇晨王维山肖业苏甜郭奇黄燕杨觅魏婕 L.Darryl Quarles 肖洲生曾超罗湘杭雷光华李长俊 department of Endocrinology Endocrinology Research CenterXiangya HospitalCentral South UniversityChangsha 410008China Hunan Key Laboratory of Joint Degeneration and Injury Changsha 410008China Key Laboratory of Aging-related Bone and Joint Diseases Prevention and Treatment Ministry of EducationXiangya HospitalCentral South UniversityChangsha 410008China School of biomedical Sciences Institute for Tissue Engineering and Regenerative MedicineFaculty of MedicineThe Chinese University of Hong KongHong Kong 999077China Key Laboratory for Regenerative Medicine Ministry of EducationSchool of Biomedical SciencesFaculty of MedicineThe Chinese University of Hong KongHong Kong 999077China Center for Neuromusculoskeletal Restorative Medicine(CNRM) The Chinese University of Hong KongHong Kong 999077China department of orthopaedics The First Affiliated Hospital of Shihezi UniversityShihezi 832061China department of orthopaedics Xiangya HospitalCentral South UniversityChangsha 410008China department of Epidemiology and Health Statistics Xiangya School of Public HealthCentral South UniversityChangsha 410008China department of Medicine University of Tennessee Health Science CenterMemphis 38163USA National clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410008China Laboratory Animal Center Xiangya HospitalCentral South UniversityChangsha 410008China

Mechanical loading is required for bone homeostasis,but the underlying mechanism is still *** previous studies revealed that the mechanical protein polycystin-1(PC1,encoded by Pkd1)is critical for bone ***,the role of PC1 in bone resorption is ***,we found that PC1directly regulates osteoclastogenesis and bone *** conditional deletion of Pkd1 in the osteoclast lineage resulted in a reduced number of osteoclasts,decreased bone resorption,and increased bone mass.A cohort study of 32,500 patients further revealed that autosomal dominant polycystic kidney disease,which is mainly caused by loss-of-function mutation of the PKD1 gene,is associated with a lower risk of hip fracture than those with other chronic kidney ***,mice with osteoclastspecific knockout of Pkd1 showed complete resistance to unloading-induced bone loss.A mechanistic study revealed that PC1 facilitated TAZ nuclear translocation via the C-terminal tail-TAZ complex and that conditional deletion of Taz in the osteoclast lineage resulted in reduced osteoclastogenesis and increased bone *** regulation of the PC1-TAZ axis alleviated unloading-and estrogen deficiency-induced bone ***,the PC1-TAZ axis may be a potential therapeutic target for osteoclast-related osteoporosis.

关键词： Polycystin1 Osteoclastogenesis Bone resorption Mechanical stress