Isolation and culture of human primary osteoblasts:Comparing the effects of differences in method details on osteoblast characteristics

作     者：Fei Chen Yan Zhang Pai Peng Xiao-Ting Huang Zi-Han Qiu Bao-Cheng Liu Tie-Lin Yang Bo Yang Yan Guo Fei Chen;Yan Zhang;Pai Peng;Xiao-Ting Huang;Zi-Han Qiu;Bao-Cheng Liu;Tie-Lin Yang;Bo Yang;Yan Guo

作者机构：Key Laboratory of Biomedical Information Engineering of Ministry of EducationBiomedical Informatics & Genomics CenterSchool of Life Science and TechnologyXi'an Jiaotong UniversityXi'anShaanxi 710049China Department of OrthopaedicsShannxi Provincial People's Hospital (The Third Affiliated Hospital of Xi'an Jiaotong University)Xi'anShaanxi 710068China 

出 版 物：《Genes & Diseases》 (基因与疾病（英文）)

年 卷 期：2024年第11卷第2期

页      面：546-549页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.82170896,31970569) Science Fund for Distinguished Young Scholars of Shaanxi Province,China(No.2021JC-02) Innovation Capability Support Program of Shaanxi Province,China(No.2022TD-44) China Postdoctoral Science Foundation(No.2021M692582) Natural Science Basic Research Program of Shaanxi Province,China(No.2022JQ-808,2023-YBSF-180) Fundamental Research Funds for the Central Universities(China)(No.xzy012022032) 

主　　题：impaired culture details 

摘      要：Osteoblasts are essential in the maintenance of human bone homeostasis.1 The abnormal formation and impaired differentiation ability of osteoblasts are pivotal factors leading to bone-related diseases,suggesting that an indepth study of osteoblasts can provide novel treatment strategies for these diseases.2 Human primary osteoblasts are precious experimental models in bone-related research.3 However,the isolation and culture of human primary osteoblasts still have some difficulties and ***,we refined a method to isolate and culture human primary osteoblasts by taking cancellous bone through a bone tissue sampler and pre-digesting it with pancreatin,which makes the process easier,cheaper,and more ***,we compared the effects of the subtle changes in isolation and culture methods on the phenotype of human primary osteoblasts and examined the differences in morphology,proliferation,and differentiation between micro-explants cultured osteoblasts(MECOBs)and explants cultured osteoblasts(EC-OBs).