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Multi-focus non-periodic scanning method for femtosecond lasers based on DMD and galvanometer scanners[Invited]

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Chinese Optics Letters 2024年第5期22卷 89-94页

作者：李华明王雨胡庆磊张卓宇吕晓华曾绍群 britton chance center for biomedical photonics Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430074China moe key laboratory for biomedical photonics Collaborative Innovation Center for Biomedical EngineeringSchool of Engineering SciencesHuazhong University of Science and TechnologyWuhan 430074China

Multi-focus parallel scanning can effectively increase laser fabrication ***,the conventional approach of using a spatial light modulator(SLM)to generate multi-foci and scan this fixed number of foci with galvanometer scanners can only achieve a periodic scanning trajectory due to the low switching speed of the *** we demonstrate a multifocus non-periodic scanning method for femtosecond lasers by using,instead,a fast-switching digital micromirror device(DMD)to generate a dynamic number of *** number of effective foci is quickly switched by introducing aberration to the undesired *** this way,the intensity allocated to each focus will not be affected by the number of foci,and a uniformity of 98%with different numbers of foci is achieved without adjusting the total laser ***,we validate the effectiveness of this scanning method by demonstrating corneal flap fabrication of porcine cornea in vitro.

关键词： multi-focus non-periodic trajectory digital micromirror device aberration control corneal cutting

Tissue optical clearing enhances efficacy of vascular targeted photodynamic therapy of mouse dorsal skin

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Journal of Innovative Optical Health Sciences 2024年第2期17卷 67-78页

作者： Ying Liu Qiushi Wang Yidi Liu Ying Wang Haixia Qiu Dan Zhu Ying Gu Defu Chen School of Optics and photonics Beijing Institute of TechnologyBeijing 100081P.R.China School of Medical Technology Beijing Institute of TechnologyBeijing 100081P.R.China Department of Laser Medicine First Medical Center of PLA General HospitalBeijing 100853P.R.China britton chance center for biomedical photonics-moe key laboratory for biomedical photonics A duanced Biomedical Imaging FacilityWuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430074HubeiP.R.China Precision Laser Medical Diagnosis and Treatment Innovation Unit Chinese A cademy of Medical SciencesBeijing 100000P.R.China

Vascular-targeted photodynamic therapy(V-PDT)is an effective treatment for port wine stains(PWS).However,repeated treatment is usually needed to achieve optimal treatment outcomes,possibly due to the limited treatment light penetration depth in the PWS *** optical clearing technique can increase light penetration in depth by reducing light *** study aimed to investigate the V-PDT in combination with an optical clearing agent(OCA)for the therapeutic enhancement of V-PDT in the rodent skinfold window chamber *** responses were closely monitored with laser speckle contrast imaging(LSCI),optical coherence tomography angiography,and stereo microscope before,during,and after the *** further quantitatively demonstrated the effects of V-PDT in combination with OCA on the blood flow and blood vessel size of skin *** combination of OCA and V-PDT resulted in significant vascular damage,including vasoconstriction and the reduction of blood *** results indicate the promising potential of OCA for enhancing V-PDT for treating vascular-related diseases,including PWS.

关键词： Vascular-targeted photodynamic therapy(V-PDT) optical clearing agent(OCA) treatment efficacy enhancement skin-fold window chamber port wine stains

MACS-W:A modified optical clearing agent for imaging 3D cell cultures

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Journal of Innovative Optical Health Sciences 2024年第2期17卷 24-34页

作者： Xiang Zhong Chao Gao Hui Li Yuening He Peng Fei Zaozao Chen Zhongze Gu Dan Zhu Tingting Yu britton chance center for biomedical photonics-moe key laboratory for biomedical photonics Huazhong University of Science and Technology Wuhan Hubei 430074P.R.China Wuhan National laboratory for Optoelectronics-Advanced biomedical Imaging Facility Huazhong University of Science and Technology Wuhan Hubei 430074P.R.China School of Optical and Electronic Infrmation Huazhong University of Science and Technology Wuhan Hubei 430074P.R.China State key laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University NanjingJiangsu 210096P.R.China Institute of Biomaterials and Medical Devices Southeast University Suzhou Jiangsu 215163P.R.China

Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal *** modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent *** tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or *** address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and *** our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor *** summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.

关键词： Tissue optical clearing 3D cell cultures imaging

Acceptor Engineering Produces Ultrafast Nonradiative Decay in NIR-Ⅱ Aza-BODIPY Nanoparticles for Efficient Osteosarcoma Photothermal Therapy via Concurrent Apoptosis and Pyroptosis

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Research 2024年第1期2024卷 625-635页

作者： Zhenxiong Shi Hua Bai Jiaxing Wu Xiaofei Miao Jia Gao Xianning Xu Yi Liu Jiamin Jiang Jiaqi Yang Jiaxin Zhang Tao Shao Bo Peng Huili Ma Dan Zhu Guojing Chen Wenbo Hu Lin Li Wei Huang Frontiers Science center for Flexible Electronics Xi’an Institute of Flexible Electronics(IFE)and Xi’an Institute of Biomedical Materials&EngineeringNorthwestern Polytechnical UniversityXi’an 710072China key laboratory for Organic Electronics and Information Displays&Institute of Advanced Materials(IAM) Nanjing University of Posts&TelecommunicationsNanjing 210023China key laboratory of Flexible Electronics(KLOFE)and IAM Nanjing Tech UniversityNanjing 211800China britton chance center for biomedical photonics-moe key laboratory for biomedical photonics Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging FacilityHuazhong University of Science and TechnologyWuhan 430074China Department of Orthopedics Xijing HospitalThe Fourth Military Medical UniversityXi’an 710032China The Institute of Flexible Electronics(IFE Future Technologies)Xiamen UniversityXiamen 361005China

Small-molecule photothermal agents(PTAs)with intense second near-infrared(NIR-Ⅱ,1,000 to 1,700 nm)absorption and high photothermal conversion efficiencies(PCEs)are promising candidates for treating deep-seated tumors such as *** date,the development of small-molecule NIR-Ⅱ PTAs has largely relied on fabricating donor–acceptor–donor(D–A–D/D′)structures and limited success has been ***,through acceptor engineering,a donor–acceptor–acceptor(D–A–A′)-structured NIR-Ⅱ aza-boron-dipyrromethene(aza-BODIPY)PTA(SW8)was readily developed for the 1,064-nm laser-mediated phototheranostic treatment of *** the donor groups to acceptor groups produced remarkable red-shifts of absorption maximums from first near-infrared(NIR-Ⅰ)regions(~808 nm)to NIR-Ⅱ ones(~1,064 nm)for aza-BODIPYs(SW1 to SW8).Furthermore,SW8 self-assembled into nanoparticles(SW8@NPs)with intense NIR-Ⅱ absorption and an ultrahigh PCE(75%,1,064 nm).This ultrahigh PCE primarily originated from an additional nonradiative decay pathway,which showed a 100-fold enhanced decay rate compared to that shown by conventional pathways such as internal conversion and vibrational ***,SW8@NPs performed highly efficient 1,064-nm laser-mediated NIR-Ⅱ photothermal therapy of osteosarcoma via concurrent apoptosis and *** work not only illustrates a remote approach for treating deep-seated tumors with high spatiotemporal control but also provides a new strategy for building high-performance small-molecule NIR-Ⅱ PTAs.

关键词： donor BODIPY intense

Tissue optical clearing imaging for structural changes of neuromuscular junctions after mice ischemic stroke[Invited]

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Chinese Optics Letters 2023年第12期21卷 54-60页

作者：徐健壹戴奕宣昂姚应涛刘少军俞婷婷朱䒟 britton chance center for biomedical photonics MoE Key Laboratory for Biomedical PhotonicsWuhan National Laboratory for OptoelectronicsAdvanced Biomedical Imaging FacilityHuazhong University of Science and TechnologyWuhan 430074China

Ischemic stroke causes long-term disability and results in motor *** impairments are associated with structural changes in the neuromuscular junction(NMJ),including detailed morphology and three-dimensional(3D)***,previous studies only explored morphological changes of individual NMJs after stroke,which limits the understanding of their role in post-stroke motor ***,we examine 3D distributions and detailed morphology of NMJs in entire mouse muscles after unilateral and bilateral strokes induced by *** results show that 3D distributions and numbers of NMJs do not change after stroke,and severe unilateral stroke causes similar levels of NMJ fragmentation and area enlargement to bilateral *** research provides structural data,deepening the understanding of neuromuscular pathophysiology after stroke.

关键词： tissue optical clearing optical imaging stroke neuromuscular junction

Plasmon-resonant gold nanoparticles for cancer optical imaging

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Science China(Physics,Mechanics & Astronomy) 2013年第3期56卷 506-513页

作者： WANG Zhen britton chance center for biomedical photonics Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology moe key laboratory for biomedical photonics Department of Biomedical EngineeringHuazhong University of Science and Technology

Gold nanoparticles(AuNPs) interact with light and have strong and tunable surface plasmon resonance,which can be detected using multiple imaging *** provide an unique opportunity for their potential applications in optical imaging for early detection of *** this review,we summarized nanoparticles targeting properties for cancer,plasmon optical properties of AuNPs,application of AuNPs for cancer optical *** discussed is the safety of AuNPs.

关键词： gold nanoparticles surface plasmon resonance optical imaging cancer

Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration

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Light(Science & Applications) 2021年第12期10卷 2492-2506页

作者： Dong-Yu Li Qing Xia Ting-Ting Yu Jing-Tan Zhu Dan Zhu britton chance center for biomedical photonics Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and Technology430074 WuhanHubeiChina moe key laboratory for biomedical photonics Huazhong University of Science and Technology430074 WuhanHubeiChina

Laser speckle contrast imaging(LSCI)is a powerful tool to monitor blood flow distribution and has been widely used in studies of microcirculation,both for animal and clinical ***,LSCI usually works on reflectivedetected ***,it could provide promising temporal and spatial resolution for in vivo applications only with the assistance of various tissue windows,otherwise,the overlarge superficial static speckle would extremely limit its contrast and ***,we systematically investigated the capability of transmissive-detected LSCI(TR-LSCI)for blood flow monitoring in thick *** Monte Carlo simulation,we theoretically compared the performance of transmissive and reflective *** was found that the reflective-detected mode was better when the target layer was at the very surface,but the imaging quality would rapidly decrease with imaging depth,while the transmissivedetected mode could obtain a much stronger signal-to-background ratio(SBR)for thick *** further proved by tissue phantom,animal,and human experiments that in a certain thickness of tissue,TR-LSCI showed remarkably better performance for thick-tissue imaging,and the imaging quality would be further improved if the use of longer wavelengths of near-infrared ***,both theoretical and experimental results demonstrate that TR-LSCI is capable of obtaining thick-tissue blood flow information and holds great potential in the field of microcirculation research.

关键词： reflective thick speckle

Organic fluorescent probes for live-cell super-resolution imaging

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Frontiers of Optoelectronics 2023年第4期16卷 25-36页

作者： Xinxin Duan Meng Zhang Yu‑Hui Zhang britton chance center for biomedical photonics MoE Key Laboratory for Biomedical PhotonicsAdvanced Biomedical Imaging Facility-Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430074China

The development of super-resolution technology has made it possible to investigate the ultrastructure of intracellular organelles by fuorescence microscopy,which has greatly facilitated the development of life sciences and *** realize super-resolution imaging of living cells,both advanced imaging systems and excellent fuorescent probes are *** fuorescent probes have good availability,but that is not the case for probes for live-cell super-resolution *** this review,we frst introduce the principles of various super-resolution technologies and their probe requirements,then summarize the existing designs and delivery strategies of super-resolution probes for live-cell imaging,and fnally provide a brief conclusion and overview of the future.

关键词： Super-resolution imaging Organic fluorescent dyes-Live-cell imaging Cell-impermeable organic probes

A feasible method for comparing the power dependent photostability of fluorescent proteins

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Chinese Optics Letters 2008年第12期6卷 941-943页

作者：刘俊储军朱宏宇徐玲玲张智红曾绍群黄振立 britton chance center for biomedical photonics Wuhan National laboratory for Optoelectronics Huazhong University of Science and Technology

A feasible method of combining the concept of fluorescence half-life and the power dependent photo- bleaching rate for characterizing the practical photostability of fluorescent proteins （FPs） was introduced. Furthermore, by using a fluorescent photostability standard, a relative comparison of the photostabilty of FPs from different research groups was proposed, which would be of great benefit for developing novel FPs with optimized emission wavelength, better brightness, and improved photostability. We used rho- damine B as an example to verify this method and evaluate the practical photostability of a far-red FP, mKate-S158C. Experimental results indicated good potential of this method for further study.

关键词： A feasible method for comparing the power dependent photostability of fluorescent proteins