Line-scanning microscopy with laterally symmetric imaging using simultaneous cross-line illumination

作     者：DAN SHEN YAFENG LI MENG WANG YUTONG HAN BOLIN LU HUI GONG QINGMING LUO JING YUAN DAN SHEN;YAFENG LI;MENG WANG;YUTONG HAN;BOLIN LU;HUI GONG;QINGMING LUO;JING YUAN

作者机构：Britton Chance Center for Biomedical Photonics and MoE Key Laboratory for Biomedical PhotonicsWuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430074China HUST-Suzhou Institute for BrainsmaticsJITRI Institute for BrainsmaticsSuzhou 215123China School of Biomedical EngineeringHainan UniversityHaikou 570228China 

出 版 物：《Photonics Research》 (光子学研究（英文版）)

年 卷 期：2024年第12卷第7期

页      面：1513-1521页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 0804[工学-仪器科学与技术] 100214[医学-肿瘤学] 10[医学] 

基　　金：Ministry of Science and Technology of the People's Republic of China (2021ZD0201001) National Natural Science Foundation of China (62325502, 81827901) Huazhong University of Science and Technology(2019kfyXMBZ011) 

主　　题：illumination simultaneous scanning 

摘      要：Using an on-the-fly scanning scheme, line confocal microscopy can obtain complex structures of large biological tissues with high throughput. Yet, it suffers from lateral imaging asymmetry and thus introduces the potential deformations of the observation results. Here, we propose cross-line illumination microscopy (cLIM) that acquires the imaging data of two perpendicular directions simultaneously through the same objective lens in a line scanning and utilizes two-direction deconvolution fusion to achieve lateral symmetric imaging *** fluorescence beads indicates that cLIM reduces lateral resolution asymmetry from 46.1%to 2.5%and improves lateral resolution by 31.0%, compared with traditional line-scanning imaging. Compared with commercial point-confocal microscopy, the cLIM has a 25.84×increase in imaging speed and 1.93×better background-suppressing ability when imaging an 11,306μm×7783μm×100μm mouse kidney slice. We also show the advantages of the cLIM in observing direction-sensitive texture features by imaging a muscular tissue slice. cLIM offers a novel solution to achieve laterally symmetric line-scanning imaging with simple modifications while maintaining high throughput and accuracy for imaging large-scale samples.