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Enzyme-metal nanocomposites for antibacterial applications

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Particuology 2022年第5期20卷 134-139页

作者： Jiarong Xiong Xinyi cai Jun Ge Key lab for Industrial Biocatalysis Ministry of EducationDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China Institute of biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China

Metal nanoparticles have been used as antibacterial agents widely,and the combined use of enzymes and metal nanoparticles promotes antibacterial activity,achieving a synergistic ***,enzymes decrease the amounts of metals and increase biocompatibility,thereby reducing toxicity of ***,the efficiency of enzymes is hindered when coupled with metals,which causes deactivation in the function of *** can a balance be struck between metals and enzymes?Although the antibacterial mechanism of metal nanoparticles is relatively clear,how enzyme-metal nanocomposites work against bacteria is not ***,we describe several examples on the synthesis of enzyme-metal nanocomposites via co-immobilization or in situ reduction and summarize how enzyme-metal nanocomposites combat microorganisms.

关键词： Enzyme-metal nanocomposites Applications Preparations Antibacterial mechanisms

Molecular basis of COVID-19,ARDS and COVID-19-associated ARDS:Diagnosis pathogenesis and therapeutic strategies

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BIOCELL 2023年第11期47卷 2335-2350页

作者： PRIYADHARSHINI THANJAVUR SRIRAMAMOORTHI GAYATHRI GOPAL SHIBI MURALIDAR SAI RAMANAN ESWARAN DANUSH NARAYAN PANNEERSELVAM BHUVANESWARAN MEIYANATHAN SRICHANDRASEKAR THUTHIKKADU INDHUPRAKASH SENTHIL VISAGA AMBI biopharmaceutical Research lab Anusandhan Kendra-1SASTRA Deemed-to-be UniversityThanjavur613401India School of Chemical and Biotechnology SASTRA Deemed-to-be UniversityThanjavur613401India

The novel coronavirus pneumonia(COVID-19)is spreading worldwide and threatening people *** routes by which SARS-CoV-2 causes lung injury have grown to be a major concern in the scientific community since patients with new Coronavirus,severe acute respiratory syndrome coronavirus(SARS-CoV-2)have a high likelihood of developing acute respiratory distress syndrome(ARDS)in severe *** mortality rate of COVID-19 has increased over the period due to rapid spread,and it becomes crucial to understand the disease epidemiology,pathogenic mechanisms,and suitable treatment *** is a respiratory disorder and is one of the clinical manifestations observed in patients with severe *** this scenario,it is important to address this problem to develop suitable treatment *** review attempts to present the prevalence of ARDS in COVID-19 patients and their predictive causes and risk factors,highlighting the contrasting features of COVID-19-induced ARDS with typical *** review also presents insights into the association between SARS-CoV-2 and lung damage while exploring the potential COVID-19 mechanisms in ARDS from the perspective of the angiotensin-converting enzyme 2 protein,cytokine storm,immune responses,and other signaling *** review also discusses the diagnosis strategies,pathogenesis,risk factors,and treatment options of COVID-19-related ARDS.

关键词： ARDS SARS-CoV-2 Variants Pathogenesis Risk factors Vaccines

Advances in photo-enzymatic-coupling catalysis system

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Systems Microbiology and Biomanufacturing 2021年第3期1卷 245-256页

作者： Yunxiu Bai Licheng Wang Jun Ge Key lab for Industrial Biocatalysis Ministry of EducationDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China Institute of biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China

Photosynthesis,as an efficient pathway for solar energy capture and utilization,has supported aerobionts for billions of *** imitation of photosynthesis to construct artificial photo-enzymatic-coupling catalysis system has become a pow-erful means to solve energy and environmental *** years of in-depth research on this coupled system,through ingenious and rational design,the synergistic effect of photo-and enzymatic catalyses has played a significant role in many different fields,including solar-driven fuel production,chiral chemical synthesis and carbon dioxide ***,light in enzymatic catalysis could also endow enzyme new ***-induced radical cofactor could bring catalytic promiscuity to enzymes,making them catalyze reactions that natural enzymes *** review summarizes the advances in photo-enzymatic-coupling catalysis system and introduces its essential components,their integration and *** possibilities presented by photo-induced catalytic promiscuity and its significance for expanding the toolbox of enzymes are also discussed.

关键词： Photo-enzymatic-coupled catalysis Artificial photosynthesis Catalytic promiscuity

A sandwich structure composite wound dressing with firmly anchored silver nanoparticles for severe burn wound healing in a porcine model

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Regenerative Biomaterials 2021年第5期8卷 37-49页

作者： Jianmin Yang Yufeng Huang Jiajia Dai Xianai Shi Yunquan Zheng Department of Biomedical Engineering College of Biological Science and EngineeringFuzhou UniversityNo.2 Xueyuan RoadFuzhou 350108China Fujian Key lab of Medical Instrument and biopharmaceutical Technology Fuzhou UniversityNo.2 Xueyuan RoadFuzhou350108China Institute of Pharmaceutical Biotechnology and Engineering College of ChemistryFuzhou UniversityNo.2 Xueyuan RoadFuzhou 350108China

Wounds may remain open for a few weeks in severe burns,which provide an entry point for pathogens and microorganisms ***,wound dressings with long-term antimicrobial activity are crucial for severe burn wound ***,a sandwich structure composite wound dressing anchored with silver nanoparticles(AgNPs)was developed for severe burn wound *** were in situ synthesized on the fibers of chitosan nonwoven fabric(CSNWF)as the interlayer of wound dressing for sustained release of silver *** firmly anchored AgNPs could prevent its entry into the body,thereby eliminating the toxicity of *** outer layer was a polyurethane membrane,which has a nanoporous structure that could maintain free transmission of water ***/collagen sponge was selected as the inner layer because of its excellent biocompatibility and *** presence of AgNPs in the CSNWF was fully characterized,and the high antibacterial activity of CSNWF/AgNPs was confirmed by against Escherichia coli,Pseudomonas aeruginosa and Staphylococcus *** superior wound healing effect on deep dermal burns of presented composite wound dressing was demonstrated in a porcine *** finding suggested that the prepared AgNPs doped sandwich structure composite wound dressing has great potential application in severe wound care.

关键词： burn injury wound dressing silver nanoparticle antimicrobial activity

Hybrid enzyme catalysts synthesized by a de novo approach for expanding biocatalysis

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Chinese Journal of Catalysis 2021年第10期42卷 1625-1633页

作者： Yufei Cao Jun Ge Key lab for Industrial Biocatalysis Ministry of EducationDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China Institute of biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055GuangdongChina

The two major challenges in industrial enzymatic catalysis are the limited number of chemical reaction types that are catalyzed by enzymes and the instability of enzymes under harsh conditions in industrial *** enzyme catalysis to a larger substrate scope and greater variety of chemical reactions and tuning the microenvironment surrounding enzyme molecules to achieve high enzyme performance are urgently *** this account,we focus on our efforts using the de novo approach to synthesis hybrid enzyme catalysts that can address these two challenges and the structure-function relationship is discussed to reveal the principles of designing hybrid enzyme *** hope that this account will promote further efforts toward fundamental research and wide applications of designed enzyme hybrid catalysts for expanding biocatalysis.

关键词： Expanding biocatalysis In situ synthesis Hybrid enzyme catalysts Structure-function relationship Rational design

Graphene-based nanomaterials for cancer therapy and anti-infections

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Bioactive Materials 2022年第8期7卷 335-349页

作者： Yan Wang Juan Li Xiaobin Li Jinping Shi Zhaotan Jiang Can Yang Zhang School of Physics Beijing Institute of TechnologyBeijing100081China Advanced Research Institute for Multidisciplinary Science Beijing Institute of TechnologyBeijing100081China Institute of biopharmaceutical and Health Engineering Shenzhen International Graduate SchoolTsinghua UniversityShenzhen518055China

Graphene-based nanomaterials(GBNMs)has been thoroughly investigated and extensively used in many biomedical fields,especially cancer therapy and bacteria-induced infectious diseases treatment,which have attracted more and more attentions due to the improved therapeutic efficacy and reduced reverse ***,as classic two-dimensional(2D)nanomaterials,have unique structure and excellent physicochemical properties,exhibiting tremendous potential in cancer therapy and bacteria-induced infectious diseases *** this review,we first introduced the recent advances in development of GBNMs and GBNMs-based treatment strategies for cancer,including photothermal therapy(PTT),photodynamic therapy(PDT)and multiple combination ***,we surveyed the research progress of applications of GBNMs in anti-infection such as antimicrobial resistance,wound healing and removal of *** mechanism of GBNMs was also ***,we concluded and discussed the advantages,challenges/limitations and perspective about the development of GBNMs and GBNMs-based ***,we think that GBNMs could be potential in clinic to promote the improvement of cancer therapy and infections treatment.

关键词： Graphene Phototherapy Cancer therapy Anti-infection 2D materials

Recent research and development of PLGA/PLA microspheres/nanoparticles: A review in scientific and industrial aspects

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Frontiers of Chemical Science and Engineering 2019年第1期13卷 14-27页

作者： Feng Qi Jie Wu Hao Li Guanghui Ma Beijing Nuokangda Pharmaceutical Technology Co. Ltd Beijing 100176 China Department of Mechanical and Aerospace Engineering University of Missouri MO 65211 USA National Key laboratory of Biochemical Engineering Key Laboratory of Biopharmaceutical Production and Formulation Engineering PLA Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 China

Poly(D,L-lactic-co-glycolic acid)(PLGA)/poly (lactic acid)(PLA) microspheres/nanoparticles are one of the most successful drug delivery systems (DDS) in lab and clinic. Because of good biocompatibility and biodegradability, they can be used in various areas, such as longterm release system, vaccine adjuvant, tissue engineering, etc. There have been 15 products available on the US market, but the system still has many problems during development and manufacturing, such as wide size distribution, drug stability issues, and so on. Recently, many new and modified methods have been developed to overcome the above problems. Some of the methods are easy to scale up, and have been available on the market to achieve pilot scale or even industrial production scale. Furthermore, the relevant FDA guidance on the DDS is still incomplete, especially for abbreviated new drug application. In this review, we present some recent achievement of the PLGA/PLA microspheres/nanoparticles, and discuss some promising manufacturing methods. Finally, we focus on the current FDA guidance on the DDS. The review provides an overview on the development of the system in pharmaceutical industry.

关键词： PLGA microspheres drug delivery system stability manufacturing

The Anti-tumor Effect Research of γδ T Cells in Nude Mice Model of Human Gastric Adenocarcinoma

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The Anti-tumor Effect Research of γδ T Cells in Nude Mice ...

Generation of a Canine-origin Neutralizing scFv Against Canine Parvovirus

浙江省免疫学会第十次学术大会

作者： Yan-Ping Ding Yan-Ling Wu Xing-jian Cheng Yoshimasa Tanaka Wen Zhang Department of biopharmaceutical Sciences College of Pharmaceutical SciencesZhejiang University of Technology lab of Molecular Immunology of Zhejiang Provincial Center for Disease Control and Prevention

Objective:To investigate the anti-tumor effect of γδ T cells on gastric adenocarcinoma SGC-7901 at cellular level in vitro and animal model in vivo,and to explore whether the c-Met antibodies and/or compounds co-administration could enhance the killing effect for tumors and influence the gene and protein expression of c-Met and ***:Firstly,peripheral blood mononuclear cells(PBMCs) were freshly isolated from healthy volunteers,and γδ T cells with high purity were obtained after selectively amplification stimulated with Zol and ***,Real Time Cell Analyser system(RTCA) was applied to measure cytotoxicity of γδ T cells on SGC-7901 cells at different effector-target ratios(E:T).Thirdly,animal model of SGC-7901 in Balb/c nude mice was established to explore the anti-tumor effect of γδ T cells alone or co-administration with c-Met antibodies and/or ***,RT-PCR and Western Blot experiments were carried out to detect the influence for c-Met and Fas ***:The purity of γδ T cells could reach 90.2%for 2-week cultivation in vitro via stimulation with Zol+*** screening results showed that γδ T cells could inhibit SGC-7901 tumor cell growth in a concentration-dependent *** vivo mouse model experiment indicated that y5 T cells could obviously inhibit the tumor growth,and the inhibition ability was enhanced after co-administration with c-Met antibodies and/or ***-PCR and Western Blot experiments showed that c-Met and Fas expressions were effectively inhibited and promoted respectively,and the effects could been enhanced after co-administration with c-Met antibodies and ***:γδ T cells showed the anti-tumor activity both in vitro and in vivo,and the effects could be enhanced after co-administration with c-Met antibodies and *** c-Met and Fas expression may be inhibited or promoted,respectively,during the process of *** study indicated that γδ T cells appear to be a promising tool for n

关键词： γδT cells gastric adenocarcinoma nude mouse model anti-tumor

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Journal of Northeast Agricultural University(English Edition) 2020年第3期27卷 45-52页

作者： Pi Xue-lei Wang Yu-yang Zou Yi-meng Guo Xiao-chen Kang Kai Wang Meng-xia Li De-shan Ren Gui-ping biopharmaceutical lab College of Life SciencesNortheast Agricultural UniversityHarbin 150030China Key laboratory of Agricultural Biological Functional Gene Northeast Agricultural UniversityHarbin 150030China

Canine parvovirus(CPV)is a high morbidity and lethality virus which causes severe enteric disease in *** an attempt to gain canine-origin neutralizing antibodies against CPV,single chain variable fragment(scFv)bacteria display libraries against the protective antigen VP2 were constructed and ***2 specific scFvs were selected following three rounds of screening *** scFvs were characterized by FCM and *** scFvs showed high affinity and specific binding to ***,the neutralizing activity of the antibody was preliminarily identified by CPV(100 TCID_(50))in vitro and scFv-2 showed the neutralizing ability with a titer of *** study generated the first canine-origin neutralizing scFv against *** neutralizing scFv would be constructed into full-length antibody in the *** study laid the foundation for the generation of an effective therapeutic reagent with long half-life and no immunological rejection for the prevention and treatment of CPV infection.

关键词： canine parvovirus VP2 protein canine-origion antibody library ScFv