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Acupotomy alleviates knee osteoarthritis in rabbit by regulating chondrocyte mitophagy via Pink1-Parkin pathway

Journal of Traditional Chinese Medicine 2024年第3期44卷 468-477页

作者： ZHU Wenting GUO Changqing DU Mei MA Yunxuan CUI Yongqi CHEN Xilin GUO Changqing the Third Affiliated Hospital of beijing University of Chinese Medicine School of acupuncture-Moxibustion and Tuina Beijing University of Chinese Medicine Department of Medical Technology Shijiazhuang Medical College

OBJECTIVE: To investigate the effect of acupotomy, on mitophagy and the Pink1-Parkin pathway in chondrocytes from rabbits with knee osteoarthritis(KOA).METHODS: A KOA model was established via the modified Videman method. Rabbits were randomly divided into a control group(CON), KOA group and KOA + acupotomy group(Acu). Rabbits in the acupotomy group were subjected to acupotomy for 4 weeks after model establishment. The behavior of the rabbits before and after intervention was recorded. Cartilage degeneration was evaluated by optical microscopy and fluorescence microscopy. The level of mitophagy was evaluated by transmission electron microscopy,immunofluorescence and enzyme-linked immunosorbent assay(ELISA). The expression of phosphatase and tensin homolog(PTEN)-induced kinase 1(Pink1)-Parkin mitophagy pathway components was evaluated by immunofluorescence, Western blotting and real-time polymerase chain ***: In rabbits with KOA, joint pain, mobility disorders and cartilage degeneration were observed, the Mankin score was increased, collagen type Ⅱ(Col-Ⅱ)expression was significantly decreased, mitophagy was inhibited, mitochondrial function was impaired, and factors associated with the Pink1-Parkin pathway were inhibited. Acupotomy regulated the expression of Pink1-Parkin pathway-related proteins, the mitophagy-related protein microtubule-associated protein-1 light chain-3,the translocase of the outer membrane, and the inner mitochondrial membrane 23; increased the colocalization of mitochondria and autophagosomes; promoted the removal of damaged mitochondria;restored mitochondrial adenosine-triphosphate(ATP) production;and alleviated cartilage degeneration in rabbits with ***: Acupotomy played a role in alleviating KOA in rabbits by activating mitophagy in chondrocytes via the regulation of proteins that are related to the Pink1-Parkin pathway.

关键词： PTEN phosphohydrolase acupuncture therapy cartilage mitophagy osteoarthritis, knee

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Inhibition of SLC26A4 regulated by electroacupuncture suppresses the progression of myocardial ischemia-reperfusion injury

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BIOCELL 2024年第4期48卷 665-675页

作者： FEI KONG QIYUAN TIAN BINGLIN KUANG LILI SHANG XIAOXIAO ZHANG DONGYANG LI YING KONG Department of Endocrinology Heilongjiang University of Chinese MedicineHarbin150040China Department of Cardiology Heilongjiang University of Chinese MedicineHarbin150040China college of Clinical Medicine Heilongjiang University of Chinese MedicineHarbin150040China Department of Nephrology Beijing Hospital of Integrated Traditional Chinese and Western MedicineBeijing100039China college of acupuncture and Massage Heilongjiang University of Chinese MedicineHarbin150040China Department of acupuncture Heilongjiang University of Chinese MedicineHarbin150040China

Introduction:Myocardial ischemia-reperfusion(IR)injury has received widespread attention due to its damaging ***(EA)pretreatment has preventive effects on myocardial IR ***26A4 is a Na+independent anion reverse transporter and has not been reported in myocardial IR ***:Toﬁnd potential genes that may be regulated by EA and explore the role of this gene in myocardial IR ***:RNA sequencing and bioinformatics analysis were performed to obtain the differentially expressed genes in the myocardial tissue of IR rats with EA *** infarction size was detected by TTC *** CK,creatinine kinase-myocardial band,Cardiac troponin I,and lactate dehydrogenase levels were determined by *** effect of SLC26A4 on cardiomyocyte apoptosis was explored by TUNEL staining and western *** effects of SLC26A4 on inﬂammation were determined by HE staining,ELISA,and real-time *** effect of SLC26A4 on the NF-κB pathway was determined by western ***:SLC26A4 was up-regulated in IR rats but downregulated in IR rats with EA *** with IR rats,those with SLC26A4 knockdown exhibited improved cardiac function according to decreased myocardial infarction size,reduced serum LDH/CK/CK-MB/cTnI levels,and elevated left ventricular ejection fraction and fractional ***26A4 silencing inhibited myocardial inﬂammation,cell apoptosis,phosphorylation,and nuclear translocation of NF-κB ***:SLC26A4 exhibited promoting effects on myocardial IR injury,while the SLC26A4 knockdown had an inhibitory effect on the NF-κB *** results further unveil the role of SLC26A4 in IR injury.

关键词： Myocardial ischemia Reperfusion SLC26A4 NF-κB pathway

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Electroacupuncture improves cognitive function in a rat model of mild traumatic brain injury by regulating the SIRT-1/PGC-1α/mitochondrial pathway

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Chinese Medical Journal 2024年第6期137卷 711-719页

作者： Bo Jin Yemei Gao Yixian Fu Suxin Zhang Ke Zhang Yibing Su Department of Neurosurgery Beijing Jishuitan HospitalCapital Medical UniversityBeijing 100035China Department of Traditional Chinese Medicine Beijing Jishuitan HospitalCapital Medical UniversityBeijing 100035China School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 100029China Department of Cardiology Peking Union Medical College HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China Department of Breast Surgical Oncology National Cancer CenterNational Clinical Research Center for CancerCancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China

Background:Mild traumatic brain injury(mTBI)is a common neurological trauma that can lead to cognitive *** sirtuin-1(SIRT-1)/peroxisome proliferator-activated receptor gamma coactivator-1α(PGC-1α)pathway has been reported to have neuroprotective effects in rats with craniocerebral *** evaluated potential mechanisms underlying electroacupuncture-mediated recovery of cognitive function after mTBI,focusing on the SIRT-1/PGC-1α/mitochondrial ***:We included forty 6-week-old male Sprague-Dawley rats in this *** were randomly divided into four groups:controlled cortical impactor(CCI,n=10),sham operation(sham,n=10),electroacupuncture-treated CCI(CCI+EA,n=10),and electroacupuncture-treated sham(sham+EA,n=10)*** was performed by assigning a random number to each rat and using a random number *** mTBI rat model was established using a controllable cortical *** therapy was performed on the back of rats,by inserting acupuncture needles to the specific acupoints and setting appropriate parameters for *** evaluated spatial learning and memory functions with the Morris water maze *** performed quantitative real-time polymerase chain reaction(qRT-PCR),western blotting,adenosine triphosphate(ATP)determination,and mitochondrial respiratory chain complex I(MRCC I)determination on rat hippocampal *** analyzed SIRT-1/PGC-1α expression levels and the results of mitochondrial function assays,and compared differences between groups using bilateral Student’s ***:Compared with the sham group,SIRT-1/PGC-1α expression was downregulated in the hippocampus of CCI group(Pacupuncture,it did not reach the levels observed in the sham group(Pacupuncture(P<0.01).In the Morris water maze,electroacupun

关键词： Electroacupuncture Cognitive impairment Sirtuin-1 Mild traumatic brain injury SIRT-1/PGC-1α/mitochondrial pathway

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Organic Integration of Traditional Chinese and Western Medicines—Future of Clinical Practice Guidelines of Integrated Traditional Chinese and Western Medicines

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Chinese Journal of Integrative Medicine 2024年第4期30卷 359-365页

作者： XIE Ying-lan HAN Fei JIN Ying-hui DING Yan-bing GUO Jing JI Dong-xiao ZHANG Teng CHENXiao-min SHANG Hong-cai Department of Traditional Chinese Medicine Encephalopathy Hubei Provincial Hospital of Traditional Chinese MedicineWuhan(430074)China Department of Traditional Chinese Medicine Encephalopathy Hubei Province Traditional Chinese Medicine Research InstituteWuhan(430074)China Xuanwu Hospital of Traditional Chinese Medicine Beijing(100000)China Center for Evidence-Based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhan(430074)China college of acupuncture and Massage College of Health and RehabilitationNanjing University of Chinese MedicineNanjing(210023)China Department of Ophthalmology Huaihe Hospital of Henan UniversityKaifengHenan Province(475000)China Ministry of Education and beijing Key Laboratory of Internal Medicine of Traditional Chinese Medicine Dongzhimen Hospital of Beijing University of Traditional Chinese Medicine/Ministry of Education and Beijing Key Laboratory of Internal Medicine of Traditional Chinese MedicineBeijing(100700)China

The transformation and implementation of clinical practice guidelines for integrated traditional Chinese medicine(TCM)and Western medicine(WM)is crucial to the adoption of medical science and technological findings and is an important way for TCM to be made available to the ***,clinical practice guidelines(CPGs)of TCM and WM integration in recent years was analyzed to clarify the current situation and problems in the existing guidelines according to the following four perspectives:(1)perspective of TCM and WM integration in guidelines,(2)diagnosis Using integrated TCM and WM,(3)integration of TCM and WM treatment,(4)promoting TCM and WM ***,the information and quality evaluation of CPGs for integrated Chinese and Western medicine in 2020–2022 were analyzed to explore the degree and methods of integration of Chinese and Western medicine *** last this study aimed to lay a foundation for the further establishment of Chinese characteristic,repeatable,and calculable clinical practice guidelines of TCM and WM integration.

关键词： clinical practice guidelines integrated traditional Chinese and Western medicine organic integration

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Can Tibetan medicine Honghua Ruyi pills relieve endometriosisassociated dysmenorrhea?Protocol for a randomized placebocontrolled trial

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Journal of Traditional Chinese Medical Sciences 2024年第1期11卷 78-85页

作者： Mei Han Jiahui Cao Jiali Wei Hui Luo Chaoqin Yu Xuefang Liang Nyangmotse Guoyan Yang Huilan Du Jianping Liu Centre for Evidence-Based Chinese Medicine Beijing University of Chinese MedicineBeijing 102488China college of Management Beijing University of Chinese MedicineBeijing 102488China college of acupuncture and Moxibustion Beijing University of Chinese MedicineBeijing 102488China Institute for Tibetan Medicine China Tibetology Research CenterBeijing 100101China Changhai Hospital Naval Medical UniversityShanghai 200433China Department of Gynaecology Guangdong Provincial Hospital of Chinese MedicineGuangdong 510120China Department of Gynaecology Tibetan Medicine Hospital of Qinghai ProvinceXining 810007China NICM Health Research Institute Western Sydney UniversityPenrithNSW 2751Australia college of Integrated Chinese and Western Medicine Hebei University of Chinese MedicineShijiazhuang 050200China

Objective:To provide high-quality clinical evidence of the efficacy of Tibetan medicine Honghua Ruyi(HHRY)pills for endometriosis-associated ***:This study constitutes a multicenter,randomized,double-blind,placebo-controlled trial encompassing a three-menstrual cycle intervention followed by a three-menstrual cycle follow-up period.A total of 164 eligible females with endometriosis-associated dysmenorrhea were randomly divided into HHRY pills and placebo groups in a 1:1 *** primary outcome included dysmenorrhea symptoms assessed using Visual Analog Scale(VAS)scores and quality of life,whereas the secondary outcome measures included the maximum VAS for non-menstrual pelvic pain,duration of pain episodes(in days),frequency and quantity of the consumption of ibuprofen sustained-release capsules(or other non-steroidal anti-inflammatory drugs),and days off work/study for staff/student due to dysmenorrhea,ovarian cyst,and/or pelvic nodule *** safety was monitored throughout the treatment *** the analyses were based on the intention-to-treat *** continuous outcomes,simple or multiple linear regressions were used to estimate the differences between the HHRY pills and placebo groups,with categorical data expressed as the number and percentage of *** were compared using the chi-square test or Fisher's exact *** predefined analysis was adjusted for concomitant treatment,a variable considered to be associated with outcomes but unaffected by treatment *** of treatment effects were reported with 95%confidence ***-tailed P values≤.05 were considered statistically ***:Positive results from this trial,upon completion would provide robust evidence for the efficacy and safety of HHRY pills in treating dysmenorrhea in patients with endometriosis.

关键词： Endometriosis Dysmenorrhea Honghua Ruyi pills Randomized controlled trial Traditional Chinese medicine

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Integration of traditional,complementary,and alternative medicine with modern biomedicine:the scientization,evidence,and challenges for integration of traditional Chinese medicine

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acupuncture and Herbal Medicine 2024年第1期4卷 68-78页

作者： Zihao Zhang Raorao Li Yu Chen Hui Yang Martin Fitzgerald Qiaochu Wang Ziwen Xu Nanxi Huang Dajun Lu Lu Luo Georgetown University Medical Center Washington DCUSA Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijingChina Institute of acupuncture and Moxibustion China Academy of Chinese Medical SciencesBeijingChina The First Clinical Medical college Guizhou University of Traditional Chinese MedicineGuiyangChina Ashdale Clinic Half Moon LaneSouth Douglas RoadCorkIreland

Traditional medicine has garnered significant global recognition,with an estimated 80%of the global population using ***,it is essential to fully understand the integration of traditional medicines into current healthcare *** review aims to provide a comprehensive overview of the standard process to modernize traditional medicine scientifically in the context of modern biomedicine,further termed here as“scientization”.Specifically,we aim to summarize the advancements made in understanding the efficacy,effectiveness,and underlying mechanisms of herbal *** also examined the transition from experience-to evidence-based medicine during ***,we explore the development of universal safety and quality control ***,we discuss international trade and export markets for Chinese medical *** development and integration of traditional medicine have allowed it to further improve human health,resulting in a more comprehensive health solution for the global population.

关键词： acupuncture Conventional medicine Herbal medicine Modernization Scientization Traditional Chinese medicine

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Electroacupuncture targeting the immune system to alleviate sepsis

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acupuncture and Herbal Medicine 2024年第1期4卷 56-67页

作者： Mengyue Fang Yuye Lan Man Li Chennan Li Bin Xu Yan Ma Sulukkana Noiprasert Xianghong Jing Lingling Yu Tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina School of Basic Medicine Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Key Laboratory of Integrated acupuncture and Medicine Research of Ministry of Education Nanjing University of Chinese MedicineNanjingChina Wuhan No.1 Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina School of Integrative Medicine Mae Fah Luang UniversityChiangraiThailand Institute of acupuncture and Moxibustion China Academy of Chinese Medical SciencesBeijingChina Institute of Integrated Traditional Chinese and Western Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

Sepsis is a life-threatening inflammatory syndrome with high morbidity and mortality ***,options for sepsis are still limited to general treatment in intensive care units(ICUs),and effective therapies that improve sepsis survival are *** disturbances play a vital role in the pathology of sepsis and are associated with protracted inflammation,susceptibility to infections,and ***,many investigators have focused on the potential benefits of immunomodulation therapy for ***(EA)has been practiced in clinics for many years and has shown advantages in treating infectious *** the last few decades,our understanding of the efficacy and mechanisms of EA in sepsis has undergone considerable *** searched the literature regarding“CNKI,Wan Fang Data,VIP Database,PubMed,and Ingenta Connect”from 2010 to 2023,using the keywords“sepsis”“septic”and“electroacupuncture”and 336 sources were ***,we included 82 studies that targeted the immune system to determine EA’s anti-inflammatory and immunomodulatory effects on *** this review,we found that EA has clinical benefits in relieving septic inflammation,improving immune function,and attenuating related multi-organ injury through several mechanisms,such as activation of the cholinergic anti-inflammatory pathway(CAP),vagaladrenal axis,inhibition of the nuclear factor Kappa-B(NF-κB)signaling pathway,signal transducers and activators of transcription(STAT)signaling pathway,and improvement of immune cell ***,EA may be a promising complementary therapy for sepsis *** also expect these data will contribute to further studies on EA in sepsis.

关键词： Cholinergic anti-inflammatory pathway Electroacupuncture Nuclear factor Kappa-B Sepsis Signal transducers and activators of transcription Vagal-adrenal axis

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Complementary and alternative medicine on cognitive defects and neuroinflammation after sepsis

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Journal of Traditional Chinese Medicine 2024年第2期44卷 408-416页

作者： LI Zhenxuan WANG Xuerui Luis Ulloa Ayman Youssef BAI Yunjing XU Xiaolong LIU Qingquan Department of Infectious Diseases Beijing Hospital of Traditional Chinese MedicineCapital Medical UniversityBeijing 100010China beijing Key Laboratory of Basic Research with Traditional Chinese Medicine on Infectious Diseases Beijing 100010China Infection immunity laboratory Beijing Institute of Traditional Chinese MedicineBeijing 100010China Clinical college of Traditional Chinese Medicine Capital Medical UniversityBeijing 100069China Center for Perioperative Organ Protection Department of AnesthesiologyDuke University Medical CenterDurhamNorth Carolina 27710USA

Sepsis-associated encephalopathy(SAE)is a common manifestation of sepsis,ranging from mild confusion and delirium to severe cognitive impairment and deep *** is associated with higher mortality and long-term outcomes,particularly substantial declines in cognitive *** mechanisms of SAE probably include neuroinflammation that is mediated by systemic inflammation and ischemic lesions in the brain,a disrupted blood–brain barrier,oxidative stress,neurotransmitter dysfunction,and severe microglial *** evidence suggests that complementary and alternative medicine,especially Traditional Chinese Medicine(TCM),is favorable in alleviating cognitive decline after ***,we summarized the studies of traditional herbal remedies,TCM formulas and acupuncture therapy in animal models of neurological dysfunctions after sepsis in recent decades and reviewed their potential mechanisms.

关键词： sepsis-associated encephalopathy cognitive dysfunction complementary therapies neuroinflammatory diseases medicine Chinese traditional review

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Clinical research progress on acupuncture for the treatment of otogenic vertigo

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World Journal of Clinical Cases 2024年第19期12卷 3676-3683页

作者： An-Na Yi Ge Yang Jia-Xin Wang Li-Qiong Zhang Peng Yuan Jing-Tian Hong Lei Zhou Department of acupuncture Jinhua Hospital of Traditional Chinese Medicine Affiliated to Zhejiang University of Chinese MedicineJinhua 321000Zhejiang ProvinceChina Department of acupuncture Beijing Hospital of Integrated Traditional Chinese and Western MedicineBeijing 100089China Pain Rehabilitation Department Yongkang Orthopedic HospitalYongkang 321300Zhejiang ProvinceChina Department of Traditional Chinese Medicine and Surgery Fujian University of Traditional Chinese MedicineFuzhou 350108Fujian ProvinceChina Department of Orthopedics and traumatology of Traditional Chinese Medicine Gansu University of Chinese MedicineLanzhou 730000Gansu ProvinceChina Department of Traditional Chinese Medicine Lanxi People's HospitalLanxi 321100Zhejiang ProvinceChina

Otogenic vertigo is a common disorder that affects the vestibular system,which often results in considerable discomfort and impaired daily *** Chinese medicine(TCM),including acupuncture and moxibustion,has been historically utilized to manage the symptoms of ***,the effectiveness and methodology of these treatments have rarely been investigated in the medical *** study reviews the existing literature on the point selection,method,and therapeutic effect of acupuncture and moxibustion to provide a reference for the TCM treatment of otogenic vertigo.A literature search was performed using the PubMed search *** terms used included otogenic vertigo,acupuncture treatment,and acupuncture point selection.A total of 34 relevant articles were retrieved from *** suggest that the clinical treatment of otogenic vertigo should consider the functions of zang-fu organs and meridians and select different acupuncture treatment methods according to syndrome differentiation based on the difference between deficiency and *** and moxibustion therapy should be based on acupoint selection,considering the syndrome differentiation,supplemented with *** treatment of otogenic vertigo with acupuncture and moxibustion refers to the selection of appropriate acupuncture methods under the guidance of TCM theory and following the principles of syndrome,disease,and meridian *** acupuncture methods include body acupuncture,auricular acupuncture,scalp acupuncture,acupoint injection,electroacupuncture,and *** are many acupuncture and moxibustion acupoints selected for the treatment of otogenic *** treatment according to the patient’s specific condition is effective and safe,which can help to improve the patient’s vertigo symptoms and cerebral blood perfusion.

关键词： Otogenic vertigo acupuncture treatment acupuncture point selection Review

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基于高频超声评价不同针刺深度治疗膝骨关节炎的临床研究

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Journal of acupuncture and Tuina Science 2024年第4期22卷 326-334页

作者：崔雨洁琪丽妲李媛媛李月馨李源胡慧李华 The Second Clinical Medical college of beijing University of Chinese Medicine Moxibustion&TuinaBeijing University of Chinese MedicineBeijing 100078China Dongfang Hospital Beijing University of Chinese MedicineBeijing 100078China

目的:观察不同针刺深度治疗膝骨关节炎(KOA)的疗效及对超声成像下积液、软骨厚度与滑膜的影响。方法:将90例KOA患者随机分为常规针刺组、西药组与浅刺组,每组30例。常规针刺组针刺深度为15-30 mm,进针后捻转片刻,以有酸麻胀触电感为佳;... 详细信息

目的:观察不同针刺深度治疗膝骨关节炎(KOA)的疗效及对超声成像下积液、软骨厚度与滑膜的影响。方法:将90例KOA患者随机分为常规针刺组、西药组与浅刺组,每组30例。常规针刺组针刺深度为15-30 mm,进针后捻转片刻,以有酸麻胀触电感为佳;浅刺组直刺2 mm,进针后不进行手法操作。以上两组取穴相同,均留针20 min,每周治疗3次。西药组给予硫酸氨基葡萄糖胶囊口服,一次2粒,每日3次。三组均治疗8周。分别在治疗前、治疗4周后和治疗8周后进行西安大略与麦克马斯特大学骨关节炎指数(WOMAC)和视觉模拟量表(VAS)评分,通过膝关节超声观察膝关节软骨、滑膜和关节积液的变化。治疗8周后进行临床疗效评价。结果:治疗8周后,常规针刺组和浅刺组积液和滑膜厚度均较治疗前显著改善(P0.05)。治疗4周和8周后,三组WOMAC(疼痛、僵硬、功能)和VAS评分均较治疗前下降(P<0.05);常规针刺组WOMAC疼痛评分均低于同时间点西药组和浅刺组(P<0.05);常规针刺组VAS评分均低于浅刺组(P<0.05)。治疗8周后,常规针刺组WOMAC中僵硬及功能评分均低于西药组(P<0.05)。结论:常规针刺、口服西药和浅刺均能缓解膝关节疼痛,改善膝关节僵硬及膝关节功能活动。常规针刺缓解膝关节疼痛疗效最佳,治疗8周后改善关节功能疗效最佳。常规针刺和浅刺均能明显减轻膝关节滑膜厚度;三种治疗方案均能改善关节积液,常规针刺和浅刺疗效更佳。

关键词：针刺疗法骨关节炎,膝针刺深度超声评价视觉模拟量表

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