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Regulatory Expression of Peroxisome Proliferator Activated Receptors Genes During Fatty Liver Formation in Geese

Agricultural Sciences in China 2010年第1期9卷 113-120页

作者： LUO Jin-biao TIAN Yong TAO Zheng-rong YUAN Qing-yan LI Guo-qin YUAN Ai-ping ZOU Li-li LU Li-zhi SHEN Jun-da SHI Fang-xiong institute of animal husbandry & veterinary Medicine Zhejiang Academy of Agricultural Sciences Hangzhou 310021 P.R.China College of animal Science and Technology Nanjing Agricultural University Nanjing 210095 P.R.China

In order to investigate the expression pattern of peroxisome proliferator activated receptor （PPAR） genes before and after overfeeding, and estimate the effect of expressed PPAR levels on weights of fatty liver and abdominal fat in geese, the RT-PCR products of PPAR genes in heart, liver, spleen, lung, kidney, stomach, small intestine, brain, breast muscle, leg muscle, and abdominal fat were determined before and after overfeeding. RT-PCR was used to determine the expression levels of PPAR genes. Quantity one software was used to analyze absorbency, and the expression level of GAPDH gene was used as contrast. Expression levels of PPAR-α were relatively high in most of detected tissues but undetectable in abdominal fat tissue before overfeeding, and the level was evidently increased in lung, appeared in abdominal fat tissue, and reduced in the other tissues after overfeeding. Expressed PPAR-γ levels were relatively high in liver, spleen, lung, small intestine, and abdominal fat, and relatively low in the other tissues before overfeeding. Expressed PPAR-γ levels were enhanced in liver, spleen, lung, stomach, and kidney but decreased in abdominal fat and without obvious changes in the other tissues. Expression patterns of PPAR genes show tissue-specific manner. In addition, expression patterns of PPAR-α are different from PPAR-γ after overfeeding. It might suggest that different functions of PPAR subtypes are responsive to overfeeding.

关键词： PPAR gene RT-PCR goose fatty liver

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Diagnosis and Treatment on Contagious Pleuropneumonia and Escherichia coli Mixed Infection in Sheep

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animal husbandry and Feed Science 2013年第2期5卷 56-59页

作者： XIA Jun YANG Xue-yun institute of veterinary research Xinjiang Academy of Animal Husbandry

[ Objective] In order to provide a reference for prevention and control of contagious pleuropneumonia and E. coli mixed infection. [ Meth- od] A comprehensive introduction of the two diseases was given on the following aspects： morbidity, clinical symptoms, pathological changes, la- boratory diagnosis, treatment, prevention, and prevention and control measures. [ Results] Sound development of sheep production was seriously influenced by the two diseases, effective prevention and control measures must be adopted to inhibit the occurrence and prevalence of sheep conta- gious pleuropneumonia and colibacillosis. [ Conclusion] The two epidemic diseases were controllable.

关键词： Sheep contagious pleuropneumonia Escherichia coli Diagnose Prevention and control measures

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Supplemental magnolol or honokiol attenuates adverse effects in broilers infected with Salmonella pullorum by modulating mucosal gene expression and the gut microbiota

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Journal of animal Science and Biotechnology 2022年第1期13卷 288-302页

作者： Fang Chen Hao Zhang Encun Du Qiwen Fan Na Zhao Feng Jin Wei Zhang Wanzheng Guo Shaowen Huang Jintao Wei institute of animal husbandry and veterinary Hubei Academy of Agricultural SciencesWuhanChina Hubei Key Laboratory of animal Embryo and Molecular Breeding WuhanChina Key Laboratory of Prevention and Control Agents for animal Bacteriosis(Ministry of Agriculture and Rural Affairs) WuhanChina

Background:Salmonella pullorum is one of the most harmful pathogens to avian *** and honokiol,natural compounds extracted from Magnolia officinalis,exerts anti-inflammatory,anti-oxidant and antibacterial *** study was conducted to evaluate the effects of dietary supplemental magnolol and honokiol in broilers infected with ***.A total of 360 one-day-old broilers were selected and randomly divided into four groups with six replicates:the negative control group(CTL),***-infected group(SP),and the *** group supplemented with 300 mg/kg honokiol(SPH)or magnolol(SPM).Results:The results showed that challenging with *** impaired growth performance in broilers,as indicated by the observed decreases in body weight(P<0.05)and average daily gains(P<0.05),along with increased spleen(P<0.01)and bursa of Fabricus weights(P<0.05),serum globulin contents,and the decreased intestine villus height and villus/crypt ratios(P<0.05).Notably,supplemental magnolol and honokiol attenuated these adverse changes,and the effects of magnolol were better than those of ***,we performed RNA-Seq in ileum tissues and 16S rRNA gene sequencing of ileum *** analysis revealed that magnolol increased the α-diversity(observed species,Chao1,ACE,and PD whole tree)and β-diversity of the ileum bacteria(P<0.05).In addition,magnolol supplementation increased the abundance of Lactobacillus(P<0.01)and decreased unidentified Cyanobacteria(P<0.05)both at d 14 and d *** study confirmed that differentially expressed genes induced by magnolol and honokiol supplementation enriched in cytokine-cytokine receptor interactions,in the intestinal immune network for IgA production,and in the cell adhesion molecule ***:Supplemental magnolol and honokiol alleviated ***-induced impairments in growth performance,and the effect of magnolol was better than that of honokiol,which could be partially due to magnolol’s abi

关键词： Broiler Gut microbiota Honokiol Immune Magnolol Salmonella pullorum

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Comparison of histopathology and PCR based assay for detection of experimentally induced toxoplasmosis in murine model

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Asian Pacific Journal of Tropical Medicine 2015年第6期8卷 448-451页

作者： Vikrant Sudan A.K.Tewari R Singh Harkirat Singh Department of Parasitology College of Veterinary Sciences & Animal HusbandryU.P.Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishuavidyalaya Evam Go Anusandhan SansthanMathura- 281001India Department of Parasitology Indian Veterinary Research Institute CADRAD.Indian veterinary research institute Department of Parasitology.GADVASU

Objective:To compare histopathology and PCR based detection in diagnosis of experimentally induced toxoplasmosis of RH human strain of the parasite in murine ***:A comparison of histopathology and PCR based detection was done to diagnose experimentally induced toxoplasmosis in ten inbred swiss albino mice after intraperitoneal inoculation of 100 tachyzoites of laboratory mantained human RH strain of the *** samples from lung,liver,spleen,brain,heart and kidney were taken and processed for histopathological examination while all the samples also were subjected to PCR,using primers directed to the multicopy of SAG 3 gene,in ***:Histopathology revealed presence of tachyzoites only in liver while along with lung,liver,spleen and brain tissue yielded desired positive PCR ***:The SAG 3 based PCR is able to diagnose toxoplasmosis in those tissues which are declared negative by histopathological assay.

关键词： Experimental infection Histopathology SAG 3 based PCR detection Toxoplasmosis

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Science Letters:Assignment of CCR7 gene to chicken chromosome 27 by radiation hybrid panel mapping

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第5期8卷 314-317页

作者： TIAN Yong LU Li-zhi FU Yan TAO Zheng-rong SHEN Jun-da WANG De-qian YUAN Ai-ping YIN Zhao-zheng Laboratory of animal Genetics College of Animal Sciences Zhejiang University Hangzhou 310029 China institute of animal husbandry and veterinary Science Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China

The protein encoded by CC chemokine receptor 7 (CCR7) is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. To map the CCR7 gene in chicken chromosome, a 6 000 rads chicken-hamster radiation hybrid panel (ChickRH6) was used. PCR of samples from ChickRH6 revealed that the location of CCR7 gene is linked to the maker SEQ0347 (6 cR away) with LOD score of 16.6 and that the marker SEQ0347 is located on chromosome 27 at 27 cR of RH (radiation hydrid) map. We compared the corresponding human mRNA sequence with the predicted coding sequence of chicken CCR7 gene, and found that the assembled contig shared a high percentage of similarity with that of the human gene.

关键词： Radiation hybrid panel Mapping CCR7 gene Chicken

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Expression analysis,single nucleotide polymorphisms within SIRT4 and SIRT7 genes and their association with body size and meat quality traits in Qinchuan cattle

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Journal of Integrative Agriculture 2016年第12期15卷 2819-2826页

作者： GUI Lin-sheng XIN Xiao-ling WANG Jia-li HONG Jie-yun ZAN Lin-sen College of animal Science and Technology Northwest A&F University Yangling 712100 P.R.China institute of animal husbandry and veterinary Medicine Henan Academy of Agricultural Scinece Zhengzhou 450008 P.R.China

Silent information regulator 2 （Sir2） proteins, or sirtuins, are nicotine adenine dinucleotide （NAD）-dependent deacetylases that connect metabolism with longevity in lower organisms. In mammals, there are seven Sir2 homologs, namely, silent information regulators （SIRT1-7）. SIRT4 and SIRT7 genes play a crucial role in regulating lipid metabolism, cellular growth and metabolism. This suggests that they are potential candidate genes for affecting body size and meat quality traits in animals. Hence, this study aimed to detect genetic variations of both SIRT4 and SIRT7 bovine genes in Qinchuan cattle, and to evaluate the effect of these variations on economically important body size and meat quality traits. Expression analysis using quantitative real-time PCR （qPCR） indicated that SIRT4 and SIRT7 were broadly expressed in all thirteen studied tissues. The expression of SIRT4 was higher in liver, muscle, and in subcutaneous fat tissue. In the case of SIRT7, the expression was higher in lung, abomasum, and subcutaneous fat. Using DNAsequencing, a total of three single nucleotide polymorphisms （SNPs） were identified within SIRT4 and SIRT7 genes in 468 Qinchuan cattle. These included one novel SNP within 3＇ untranslated regions （UTR） of SIRT4 （SNP1： g. 13915A〉G） and two novel synonymous substitutions in SIRT7 （SNP2： g.3587C〉T and SNP3： g.3793T〉C）. Statistical analyses indicated that all three SNPs could significantly influence some body size and meat quality traits in Qinchuan cattle. These novel findings will provide a background for application of bovine SIRT4 and SIRT7 genes in the selection program of Chinese cattle.

关键词： SIRT4 SIRT7 single nucleotide polymorphisms (SNPs) beef cattle

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Tributyrin administration improves intestinal development and health in pre-weaned dairy calves fed milk replacer

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animal Nutrition 2022年第3期10卷 399-411页

作者： Shuai Liu Junda Wu Zhaohai Wu Gibson Maswayi Alugongo Muhammad Zahoor Khan Jinghui Li Jianxin Xiao Zhiyuan He Yulin Ma Shengli Li Zhijun Cao State Key Laboratory of animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China institute of animal husbandry and veterinary Guizhou Academy of Agricultural SciencesGuizhou 550005China State Key Laboratory of animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China Department of animal Science University of CaliforniaDavisCA 95616USA Faculty of veterinary and animal Sciences University of AgricultureDera Ismail Khan29220Pakistan

Butyrate and its derivatives possess various nutritional and biological benefits for mammals,whereas its effects on dairy calves have not been well *** study evaluated the effects of tributyrin administration on blood immune,intestinal immune and barrier functions,and microbial composition of pre-weaned dairy *** newborn Holstein bull calves were randomly assigned into a control group(no tributyrin supplementation,CON;n=10)or a treatment group(supplemented with tributyrin at 2 g/L of milk,TRB;n=10).The results showed that diarrhea frequency was decreased significantly by tributyrin administration from d 29 to 56(P3.5,P<0.05),which was negatively associated with inflammatory gene expression(TLR2 and IL-1b)but positively associ

关键词： Dairy calves Tributyrin Gut microbiota Inflammation Barrier function

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Recent progress in Michael addition-based fluorescent probes for sulfur dioxide and its derivatives

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Chinese Chemical Letters 2018年第10期29卷 1456-1464页

作者： Junchao Xu Houqun Yuan Lintao Zeng Guangming Bao institute of veterinary Drug Jiangxi Agricultural University/Jiangxi Provincial Key Laboratory for Animal Health School of Chemistry and Chemical Engineering Tianjin University of Technology

Sulfur dioxide（SO_2） is a harmful environmental pollutant. Inhaled SO_2 can be rapidly hydrated into its derivatives, bisulfite（HSO_3^-） and sulfite（SO_3^（2-））. SO_2 derivatives are well known as preservatives and antioxidants, which are used in food and beverages to prevent oxidation and bacterial growth. Although SO_2 can be endogenously generated in mammals and exhibits unique bioactivities in regulating cardiovascular function, excessive SO_2 and its derivatives have toxic effects on humans and animals for triggering adverse reactions and diseases. A large number of fluorescent probes for SO_2 and its derivatives have been designed and reported due to their high sensitivity and selectivity, high temporal and spatial resolution, non-invasive and non-destructive detection as well as real-time visualization in situ. In this review, we have summarized the recent progress of Michael addition-based fluorescent probes for SO_2 and its derivatives. These probes are categorized and concluded according to the different α,β-unsaturated compounds（i.e., Michael acceptors）. The design strategies, sensing performances, detection mechanisms and applications of these probes are discussed in detailed. Finally, a general overview about the design of probes for SO_2 and its derivatives is provided, which will facilitate the development of ideal probes for SO_2 and its derivatives.

关键词： Fluorescent probes Michael addition Sulfur dioxide Bisulfite Sulfite Application

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Anas platyrhynchos BLVRA Gene:Molecular Cloning and Correlation between Green Shell Character and mRNA Expression in the Shell Gland in the Duck

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畜牧兽医学报 2011年第B12期42卷 13-19页

作者： ZHANG Bao-le LI Guo-qin ZHANG He SHEN Jun-da LI Jin-jun TAO Zheng-rong CHEN Yi-chu WANG De-qian CHEN Wen-biao SHI Fang-xiong LU Li-zhi institute of animal husbandry and veterinary Medicine Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China College of animal Science and Technology Nanjing Agricultural University Nanjing 210095 China Jinyun husbandry and veterinary Bureau Jinyun 321403 China Zhejiang Wulian Agriculture And animal Co. Ltd. Jinyun 321403 China

This study has examined the structure and function of the biliverdin reductase A (BLVRA) gene and its correlation with the green eggshell character in Jinyun ducks. The previously unknown sequences of 3' and 5' ends of duck BLVRA mRNA were cloned, using RT-PCR and 5' RACE methods with four pairs of primers designed from the known coding sequence (CDS) of the duck BLVRA gene. Gene expression levels of BLVRA in the shell gland were quantified by real-time PCR, and eggshell colors were determined objectively by the reflection coefficient. The cDNA sequence of BLVRA was comprised of 1071 nucleotides, encoding a protein of 303 amino acids with an isoelectric point of 7.15 and molecular weight of 34.3 kDa. The 5' and 3' ends of the duck BLVRA gene sequence published in the GenBank were extended by 238 and 199 bp, respectively. The deduced amino acid sequence of duck BLVRA had 95.3% identity with Gallus gallus, 95.0% with Taeniopygia guttata, 70.0% with Xenopus tropicalis,61.1% with Bos taurus, 60.5% with Homo sapiens and 59.5% with Mus musculus, which indicated that the BLVRA gene was evolutionarily conserved. The relative gene expression level of BLVRA in shell glands of Jinyun ducks with white eggshells was 3 times higher than that in ducks with dark green eggshells (P<0.01). There was a significant positive correlation between reflection coefficient of eggshells and the relative expression of the BLVRA gene (r=0.719, P<0.05). Together, these findings suggest that the BLVRA gene could be a candidate gene for the molecule breeding of ducks with green eggshells.

关键词：基因设计绿头鸭 mRNA表达克隆 VRA 基因表达水平 GenBank 氨基酸序列

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Prospects of diagnostic and prognostic biomarkers of pyometra in canine

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Asian pacific Journal of Reproduction 2020年第4期9卷 166-173页

作者： Laishram Kipjen Singh Manas Kumar Patra Girish Kumar Mishra Abhishek Chandra Saxena Ujjwal Kumar De Sanjay Kumar Singh Harendra Kumar Krishnaswamy Narayanan animal Reproduction Division Indian Veterinary Research InstituteIzatnagarU.P.243122India animal Reproduction Gynaecology and Obstetrics Division ICAR-National Dairy Research InstituteKarnal-132001India Department of veterinary Gynaecology andObstetrics College of Veterinary Sciences and Animal HusbandryAnjoraDurg(C.G.)India Referral veterinary Polyclinic ICAR-Indian Veterinary Research InstituteIzatnagarU.P.243122India ICAR-Indian veterinary Reseach institute HebbalBangaluru 560024India

Pyometra is one of the most common uterine pathologies of intact bitch at middle to advanced age. In the early stages, the disease shows subtle changes, making diagnosis a challenge. In contrast, at later stages, it manifests as potentially life-threatening systemic inflammatory response syndrome. Ultrasonographic examination of the uterus aids in the diagnosis, although it has limitation in ascertaining the clinical severity of pyometra. Moreover, differentiation of cystic endometrial hyperplasia from pyometra could not be discerned with greater accuracy. Therefore, false negative diagnosis of pyometra patients leads to development of systemic inflammatory response, which delays administration of therapies and results in deaths during early course of treatment. Further, indiscriminate use of broad-spectrum antimicrobials at higher dose in false positive cases considerably contributes to the rising pool of drug resistant pathogens, thereby increasing the risk of case fatality due to sepsis in a long-term. Monitoring the circulating pro-inflammatory cytokines, acute phase proteins, endotoxin, growth factors and inflammatory mediators is the current trend in pyometra research, especially for developing diagnostic and prognostic biomarkers. The present review deals with the prospects of developing diagnostic and prognostic biomarkers in the canine pyometra.

关键词： Pyometra Systemic inflammatory response Acute-phase proteins Prostaglandin F metabolite Canine

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