Comparison of histopathology and PCR based assay for detection of experimentally induced toxoplasmosis in murine model
Comparison of histopathology and PCR based assay for detection of experimentally induced toxoplasmosis in murine model作者机构：Department of ParasitologyCollege of Veterinary Sciences & Animal HusbandryU.P.Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishuavidyalaya Evam Go Anusandhan SansthanMathura- 281001India Department of ParasitologyIndian Veterinary Research Institute CADRAD.Indian Veterinary Research Institute Department of Parasitology.GADVASU
出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)
年 卷 期：2015年第8卷第6期
页 面：448-451页
核心收录：
学科分类：100208[医学-临床检验诊断学] 1002[医学-临床医学] 10[医学]
基 金：ICAR
主 题：Experimental infection Histopathology SAG 3 based PCR detection Toxoplasmosis
摘 要：Objective:To compare histopathology and PCR based detection in diagnosis of experimentally induced toxoplasmosis of RH human strain of the parasite in murine ***:A comparison of histopathology and PCR based detection was done to diagnose experimentally induced toxoplasmosis in ten inbred swiss albino mice after intraperitoneal inoculation of 100 tachyzoites of laboratory mantained human RH strain of the *** samples from lung,liver,spleen,brain,heart and kidney were taken and processed for histopathological examination while all the samples also were subjected to PCR,using primers directed to the multicopy of SAG 3 gene,in ***:Histopathology revealed presence of tachyzoites only in liver while along with lung,liver,spleen and brain tissue yielded desired positive PCR ***:The SAG 3 based PCR is able to diagnose toxoplasmosis in those tissues which are declared negative by histopathological assay.
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