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Reversing anther thermotolerance by manipulating the cis-elements in the promoter of a high-temperature upregulated gene Casein Kinase I in upland cotton

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Science China Life Sciences 2025年

作者： Yanlong Li Yinuo Fu Yaoyao Li Rui Zhang Jing Yang Huanhuan Ma Ling Min Xianlong Zhang national Key laboratory of Crop {2. improvement Huazhong Agricultural University

High temperature (HT) stress causes male sterility,leading to reduced upland cotton ***,we identified a key gene,Casein Kinase I (GhCKI),that negatively regulates male fertility in upland cotton under ***,conventional genetic manipulations of GhCKI would result in male sterility,hindering its utilization in breeding ***,we engineered quantitative variation for anther thermotolerance-related traits in upland cotton by creating weak promoter alleles of GhCKI genes,using CRISPR/Cas9 and CRISPR/Cpf1genome ***,we screened and identified two new upland cotton plant lines exhibiting a HT-tolerant phenotype with edited GhCKI promoters,and characterized their corresponding heat-tolerant allelic *** research revealed that the primary reason for the HT tolerance of the GhCKI promoter editing mutants is that the trans-acting factors GhMYB73 and GhMYB4,which positively regulate GhCKI expression under HT,failed to bind and activate the expression of ***,our study not only provides a rapid strategy to generate new beneficial alleles but also offers novel germplasm resources and molecular insights for crop HT tolerance breeding.

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Aerodynamic Optimization and Flow Mechanism for a Compressor Cascade at Low Reynolds Number

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Journal of Thermal Science 2025年第1期34卷 92-109页

作者： LI Lin ZHANG Jianshe CHEN Caiyan TAN Wei ZHANG Yanfeng Key laboratory of Light-Duty Gas-Turbine Institute of Engineering ThermophysicsChinese Academy of Sciences University of Chinese Academy of Sciences national Key laboratory of Science and Technology on Advanced Light-duty Gas-turbine

Low-Reynolds-number effect is an important factor affecting the performance of compressors and other main components of high-altitude long-endurance unmanned aerial *** improve the flow condition and reduce flow loss under the condition of high altitude and low Reynolds number(Re),this paper proposes an optimization process based on a surrogate model,which combines the class-shape transformation method(CST),the Latin hypercube sampling(LHS) method,the light gradient-boosting machine algorithm(LightGBM),and a genetic algorithm(GA) to optimize a high-subsonic compressor *** surrogate model is verified to be accurate and can be used in the optimization *** accuracy of the GA is higher than the other algorithms under common test *** optimization results are verified by numerical simulation,and the flow differences before and after optimization are compared,especially the flow within the boundary *** changing the blade shape,the optimization process adjusts the loading distribution to delay the transition of the optimized blade on the suction surface,which changes the turbulent reattachment in the laminar separation bubble(LSB) into laminar ***,the mixing loss induced by the turbulent reattachment of the LSB and the wake loss of turbulent separation at the trailing edge are significantly reduced,and the performance of the compressor profile is finally *** addition,turbulent separation of the optimized profile is delayed reducing the range of the wake region on the suction *** this optimization process,the reduction of total pressure loss coefficient at Re of 2.5×10～5,3.5×10～5,and 4.5×10～5 are 16.32.,2..76%,and 2..16%,respectively.

关键词： Reynolds number

Epigenetics in the modern era of crop improvements

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Science China Life Sciences 2025年

作者： Yan Xue Xiaofeng Cao Xiangsong Chen Xian Deng Xingwang Deng Yong Ding Aiwu Dong Chengguo Duan Xiaofeng Fang Lei Gong Zhizhong Gong Xiaofeng Gu Chongsheng He Hang He Shengbo He Xinjian He Yan He Yuehui He Guifang Jia Danhua Jiang Jianjun Jiang Jinsheng Lai Zhaobo Lang Chenlong Li Qing Li Xingwang Li Bao Liu Bing Liu Xiao Luo Yijun Qi Weiqiang Qian Guodong Ren Qingxin Song Xianwei Song Zhixi Tian Jiawei Wang Yuan Wang Liang Wu Zhe Wu Rui Xia Jun Xiao Lin Xu Zhengyi Xu Wenhao Yan Hongchun Yang Jixian Zhai Yijing Zhang Yusheng Zhao Xuehua Zhong Daoxiu Zhou Ming Zhou Yue Zhou Bo Zhu Jiankang Zhu Qikun Liu College of Life Sciences Institute of Life Science and Green Development Hebei University Biotechnology Research Institute Chinese Academy of Agricultural Sciences College of Biology Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation Hunan Engineering and Technology Research Center of Hybrid Rapeseed Hunan University Institute of Advanced Agricultural Sciences School of Advanced Agricultural Sciences Peking University State Key laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University national Institute of Biological Sciences Key laboratory of Seed Innovation Institute of Genetics and Developmental Biology Chinese Academy of Sciences School of Advanced Agricultural Sciences Peking-Tsinghua Center for Life Sciences Peking University Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular Sciences Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education College of Chemistry and Molecular Engineering Peking University Key laboratory of Seed Innovation State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences State Key laboratory of Wheat improvement Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences in Weifang State Key laboratory of Crop Stress Adaptation and improvement School of Life Sciences Henan University State Key laboratory of Maize Bio-breeding National Maize Improvement Center Department of Plant Genetics and Breeding China Agricultural University Frontiers Science Center for Molecular Design Breeding China Agricultural University Center for Crop Functional Genomics and Molecular Breeding China Agricultural University Sanya Institute of China Agricultural University Hainan Yazhou Bay Seed laboratory Institute of Advan

Epigenetic mechanisms are integral to plant growth, development, and adaptation to environmental stimuli. Over the past two decades,our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body of knowledge on both locus-specific mechanisms and genome-wide regulatory patterns. Studies initially grounded in the model plant Arabidopsis have been broadened to encompass a diverse array of crop species, revealing the multifaceted roles of epigenetics in physiological and agronomic traits. With recent technological advancements, epigenetic regulations at the single-cell level and at the large-scale population level are emerging as new focuses. This review offers an in-depth synthesis of the diverse epigenetic regulations, detailing the catalytic machinery and regulatory functions. It delves into the intricate interplay among various epigenetic elements and their collective influence on the modulation of crop traits. Furthermore, it examines recent breakthroughs in technologies for epigenetic modifications and their integration into strategies for crop improvement. The review underscores the transformative potential of epigenetic strategies in bolstering crop performance, advocating for the development of efficient tools to fully exploit the agricultural benefits of epigenetic insights.

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Repression of microRNA 160 results in retarded seed integument growth and smaller final seed size in cotton

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The Crop Journal 2020年第4期8卷 602-612页

作者： Nian Liu Shuchang Wu Zhonghua Li Anam Qadir Khan Haiyan Hu Xianlong Zhang Lili Tu national Key laboratory of Crop {2. improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Key laboratory of Biology and genetic {2. of Oil crops Ministry of Agriculture and Rural AffairsOil Crops Research Institute of the Chinese Academy of Agricultural SciencesWuhan 430062HubeiChina

microRNA 160(miR160),targeting auxin response factors(ARFs),plays many roles in plant *** investigated the role of the mi R160/ARF axis in regulation of cotton seed *** mi R160 activity,specifically in the seed coat,led to smaller seeds and less fiber production owing to attenuated growth of the maternal *** electron microscopy and histology showed that expansion of cells in the integument was retarded in mi R160-suppressed *** Gh ARF genes were targeted by mi R160 and were upregulated in mi R160-suppressed lines,indicating that a mi R160/ARF axis is present in *** genes(Ghir_A05 G003740,Scaffold1878 G000010,Ghir_D09 G02.980,Ghir_A11 G010730,and Ghir_A05 G041590),associated with reduced seed development were downregulated in mi R160-suppressed *** results suggest that the mi R160/ARF axis controls maternal integument growth to influence seed size by directly or indirectly regulating seed development-associated genes.

关键词： size cotton maternal

Complete organelle genomes of Sinapis arvensis and their evolutionary implications

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The Crop Journal 2020年第4期8卷 505-514页

作者： Shifei Sang Hongtao Cheng Desheng Mei Li Fu Hui Wang Jia Liu Wenxiang Wang Qamar U.Zaman Kede Liu Qiong Hu Oil crops Research Institute of Chinese Academy of Agricultural Sciences/Key laboratory for Biology and {2. improvement of Oil crops Ministry of Agriculture and Rural AffairsWuhan 430062HubeiChina national Key laboratory of Crop {2. improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Sinapis arvensis,belonging to the genus Sinapis of the family Brassicaceae,has good agronomic characters that make it a valuable genetic resource for crop improvement and is a cytoplasmic source of heterologous cytoplasmic male sterility(CMS).In addition,*** has played an important role in the evolution of the six major cultivated Brassica species involved in the triangle of *** next-generation sequencing,we assembled and revealed the gene composition of *** cytoplasmic *** chloroplast genome comprises 153,590 bp,with 112.individual genes,including 4 r RNA,2. t RNA,and 79 proteincoding *** mitochondrial genome comprises 2.0,02. bp,with 54 genes,including 18 t RNA,three r RNA and 33 protein-coding *** structure and evolutionary analysis indicated that the sequences of the *** organellar genomes were more similar to those of Brassica nigra and *** than to those of other Brassicaceae *** mitochondrial open reading frames displaying chimeric structural features and encoding hypothetical proteins with transmembrane domains may account for the infertility of Nsa CMS previously derived from somatic cell hybridization between *** and *** results will not only contribute to utilize the germplasm resource of ***,and comprehend the evolution of organelle genomes within the Brassicaceae family,but also help to identify genes conditioning the alloplasmic male sterility of Nsa CMS in ***.

关键词： species. organelle evolutionary

Genome-wide identification, evolution, and expression analysis of RNA-binding glycine-rich protein family in maize

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Journal of Integrative Plant Biology 2014年第10期56卷 1020-1031页

作者： Jianhua Zhangy Yanxin Zhaoy Hailin Xiao Yonglian Zheng Bing Yue national Key laboratory of Crop {2. improvement Huazhong Agricultural University

The RNA‐binding glycine‐rich protein（RB‐GRP）family is characterized by the presence of a glycine‐rich domain arranged in（Gly）n‐X repeats and an RNA‐recognition motif（RRM）. RB‐GRPs participate in varied physiological and biochemical processes especially in the stress response of plants. In this study, a total of 2. RB‐GRPs distributed on 10 chromosomes were identified in maize（Zea mays L.）, and they were divided into four subgroups according to their conserved domain architecture. Five pairs of paralogs were identified,while none of them was located on the same chromosomal region, suggesting that segmental duplication is predominant in the duplication events of the RB‐GRPs in maize. Comparative analysis of RB‐GRPs in maize, Arabidopsis（Arabidopsis thaliana L.）, rice（Oryza sativa L.）, and wheat（Triticum aestivum）revealed that two exclusive subgroups were only identified in maize. Expression of eight ZmRB‐GRPs was significantly regulated by at least two kinds of stresses. In addition, cis‐elements predicted in the promoter regions of the ZmRB‐GRPs also indicated that these ZmRB‐GRPs would be involved in stress response of maize. The preliminary genome‐wide analysis of the RB‐GRPs in maize would provide useful information for further study on the function of the ZmRB‐GRPs.

关键词： Gene expression maize motif prediction phylogenetic analysis RNA‐binding glycine‐rich proteins

Genome-wide association study of callus induction variation to explore the callus formation mechanism of rice

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Journal of Integrative Plant Biology 2019年第11期61卷 1134-1150页

作者： Zhaoyang Zhang Hu Zhao Wei Li Jiemin Wu Zaihui Zhou Fei Zhou Hao Chen Yongjun Lin national Key laboratory of Crop {2. improvement Huazhong Agricultural University

Rice(Oryza sativa) is one of the most widely cultivated food crops, worldwide. Tissue culture is extensively used in rice breeding and functional genome research. The ability to induce callus determines whether a particular rice variety can be subjected to tissue culture and Agrobacterium-mediated transformation. Over the past two decades, many quantitative trait loci(QTLs)related to callus induction traits have been identified;however, individual genes associated with rice callus induction have not been reported. In this study, we characterized three callus-induction traits in a global collection of 510 rice accessions. A genome-wide association study of the rice population in its entirety as well as subpopulations revealed 2. significant loci located in rice callus induction QTLs. We identified three candidate callus induction genes, namely CRL1, Os BMM1, and Os SET1, which Rese are orthologs of Arabidopsis LBD17/LBD2., BBM, and SWN,respectively, which are known to affect callus ***, we predicted that 14 candidate genes might be involved in rice callus induction and showed that RNA interference(RNAi)-mediated disruption of Os IAA10 inhibited callus formation on tissue culture *** growth in the Os IAA10 RNAi line was not inhibited by synthetic auxin(2.4-D) treatment, suggesting that Os IAA10 may perceive auxin and activate the expression of downstream genes, such as CRL1, to induce callus formation. The significant loci and candidate genes identified here may provide insight into the mechanism underlying callus formation in rice.

关键词： breeding crops

genetic dissection of root morphological traits as related to potassium use efficiency in rapeseed under two contrasting potassium levels by hydroponics

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Science China(Life Sciences) 2019年第6期62卷 746-757页

作者： Xiaoling Dun Jiaqin Shi Huiping Liu Jie Wang Xinfa Wang Hanzhong Wang Oil crops Research Institute of the Chinese Academy of Agricultural Sciences/Key laboratory of Biology and {2. improvement of Oil crops Ministry of Agriculture

To reveal the genetic basis of potassium use efficiency(KUE) in rapeseed, root morphology(RM), biomass and KUE-related traits were measured in a recombinant inbred line population with 175 F7 lines that were subjected to high-potassium(HK) and low-potassium(LK) treatments by hydroponics. A total of 109 significant QTLs were identified to be associated with the examined traits. Sixty-one of these QTLs were integrated into nine stable QTLs. The higher heritability for RM and biomass traits and lower heritability for KUE-related traits, as well as nine stable QTLs for RM traits and only two for KUE-related traits,suggested that regulating RM traits would be more effective than selecting KUE traits directly to improve KUE by markerassisted selection. Furthermore, the integration of stable QTLs identified in the HK, LK, high-nitrogen(HN) and low-nitrogen(LN) conditions gave 10 QTL clusters. Seven of these clusters were classified into major QTLs that explained 7.4%–2..7% of the total phenotypic variation. Five of the major QTL clusters were detected under all of the treated conditions, and four clusters were specifically detected under the LK and LN conditions. These common and specific QTL clusters may be useful for the simultaneous improvement of multiple traits by marker-assisted selection.

关键词： Brassica napus L. root traits potassium efficiency QTL mapping QTL cluster

Comparison of transcriptomes undergoing waterlogging at the seedling stage between tolerant and sensitive varieties of Brassica napus L.

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Journal of Integrative Agriculture 2015年第9期14卷 1723-1734页

作者： ZOU Xi-ling ZENG Liu LU Guang-yuan CHENG Yong XU Jin-song ZHANG Xue-kun Key laboratory of Biology and genetic {2. of Oil crops Ministry of Agriculture/Oil Crops Research InstituteChinese Academy of Agricultural Sciences

RNA sequencing of the sensitive GH01 variety of Brassica napus L. seedling roots under 12.h of waterlogging was compared with previously published data of the ZS9 tolerant variety to unravel genetic mechanisms of waterlogging tolerance beyond natural variation. A total of 2.977 genes with similar expression patterns and 17 genes with opposite expression patterns were identiifed in the transcription proifles of ZS9 and GH01. An additional 1 438 genes in ZS9 and 1 861 genes in GH01 showed strain speciifc regulation. Analysis of the overlapped genes between ZS9 and GH01 revealed that waterlogging tolerance is determined by ability to regulate genes with similar expression patterns. Moreover, differences in both gene expression proifles and abscisic acid (ABA) contents between the two varieties suggest that ABA may play some role in waterlogging tolerance. This study identiifes a subset of candidate genes for further functional analysis.

关键词： rapeseed(Brassica napus L.) waterlogging digital gene expression(DGE) roots transcriptome comparative analysis