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Neuroscience Bulletin 2023年第9期39卷 1375-1395页

作者： Yuxing Zhang Xin Zhao Chun Guo Ying Zhang Fukang Zeng Qian Yin Zhong Li Le Shao Desheng Zhou Lijuan Liu Department of Neurology The First Hospital of Hunan University of Chinese MedicineChangsha410007China Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases Changsha410208China Hunan University of Chinese Medicine Changsha410006China Laboratory of Prevention and Transformation of Major Diseases in Internal Medicine of Traditional Chinese Medicine Changsha410007China

Ischemic stroke is a major public health problem *** the circadian clock is involved in the process of ischemic stroke,the exact mechanism of the circadian clock in regulating angiogenesis after cerebral infarction remains *** the present study,we determined that environmental circadian disruption(ECD)increased the stroke severity and impaired angiogenesis in the rat middle cerebral artery occlusion model,by measuring the infarct volume,neurological tests,and angiogenesis-related *** further report that Bmal1 plays an irreplaceable role in *** of Bmal1 promoted tube-forming,migration,and wound healing,and upregulated the vascular endothelial growth factor(vegf)and Notch pathway protein *** promoting effect was reversed by the Notch pathway inhibitor DAPT,according to the results of angiogenesis capacity and vegf pathway protein *** conclusion,our study reveals the intervention of ECD in angiogenesis in ischemic stroke and further identifies the exact mechanism by which Bmal1 regulates angiogenesis through the vegf-Notch1 pathway.

关键词： Circadian clock Ischemic stroke Angiogenesis vegf Notch pathway

The combination of EGCG with warfarin reduces deep vein thrombosis in rabbits through modulating HIF-1αand vegf via the PI3K/AKT and ERK1/2 signaling pathways

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Chinese Journal of Natural Medicines 2022年第9期20卷 679-690页

作者： LI Yan GE Jing-Ping MA Ke YIN Yuan-Yuan HE Juan GU Jian-Ping Department of Vascular and Interventional Radiology Nanjing First HospitalNanjing Medical UniversityNanjing 210006China Department of Acupuncture Qinhuai District Hospital of Traditional Chinese MedicineNanjing 210000China

Deep venous thrombosis(DVT)poses a major challenge to public health *** cell injury evokes inflammatory and oxidative responses that contribute to thrombus *** polyphenol(TP)in the form of epigallocatechin-3-gallate(EGCG)has anti-inflammatory and oxidative effect that may ameliorate ***,the precise mechanism remains incompletely *** current study was designed to investigate the anti-DVT mechanism of EGCG in combination with warfarin(an oral anticoagulant).Rabbits were randomly divided into five groups.A DVT model of rats was established through ligation of the inferior vena cava(IVC)and left common iliac vein,and the animals were orally administered with EGCG,warfarin,or vehicle for seven *** vitro studies included pretreatment of human umbilical vein endothelial cells(HUVECs)with different concentrations of EGCG for 2 h before exposure to hydrogen *** weight and length were *** changes were observed by hematoxylin-eosin *** samples were collected for detecting coagulation function,including thrombin and prothrombin times,activated partial thromboplastin time,and fibrinogen *** expression in thrombosed IVCs and HUVECs was evaluated by Western blot,immunohistochemical analysis,and/or immunofluorescence ***-qPCR was used to determine the levels of AGTR-1 and vegf mRNA in IVCs and *** viability of HUVECs was examined by CCK-8 *** cytometry was performed to detect cell apoptosis and ROS generation was assessed by 2′,7ʹ-dichlorofluorescein diacetate *** vitro and in vivo studies showed that EGCG combined with warfarin significantly reduced thrombus weight and length,and apoptosis in *** findings indicated that the combination of EGCG and warfarin protects HUVECs from oxidative stress and prevents ***,HIF-1αsilencing weakened these effects,which indicated that HIF-1αmay participate in ***,HIF-1αsilencin

关键词： Deep vein thrombosis EGCG Warfarin HIF-1α vegf PI3K/AKT ERK1/2

Role of vegf-A/C and CCR-7 in the enhanced metastasis of A549 cells induced by 2 and 4 Gy X-rays in vitro and in vivo

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Science China(Technological Sciences) 2014年第5期57卷 990-997页

作者： LV Jin JIANG QiSheng SONG XiuJun WANG CuiLan GUO LiJie WANG SiNian LI FengSheng HU WenWei The Second Artillery General Hospital the Chinese People's Liberation Army

Radiotherapy is a common strategy in treating lung *** evidence suggested that radiotherapy has the potential to promote the metastasis and invasion of carcinoma *** this study,we aimed to testify the role of vascular endothelial growth factor(vegf)and C-C chemokine receptor-7(CCR-7)in the metastasis of human adenocarcinoma A549 cells in vivo and in *** mice were injected with A549 cells irradiated by 0,2 and 4 Gy X-rays,*** detections of vegf-A/C and CCR-7 mRNA from lung sample were performed by real-time *** interfering RNA(siRNA)transfection technology was further used to testify the role of the genes in the metastasis of A549 *** and CCR-7mRNA could only be detected 10 weeks post injection in vivo when visible tumor foci scattered in *** addition,vegf-A/C mRNA expressed significantly higher in mice injected with A549 cells irradiated by 2 and 4 X-rays than those with 0 Gy X-rays *** the other hand,A549 cells with or without X-rays irradiation transfected with vegf siRAN and CCR-7 siRNA showed a dramatic decrease of invasiveness compared to normal A549 cells with or without *** migration indexes were?0.7,?0.48,?0.34 and?0.32 for A549 cells with CCR-7 siRNA,vegf siRNA,X-rays combined CCR-7 siRNA and X-rays combined vegf-siRNA *** results demonstrated that X-rays could promote the metastasis of A549 cells,and vegf-A/C and CCR-7 mRNA were closely related to the metastasis of A549 cells in vivo and in vitro.

关键词： metastasis lung cancer vegf CCR-7 irradiation

Microvesicle-delivery miR-150 promotes tumorigenesis by up-regulating vegf,and the neutralization of miR-150 attenuate tumor development

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Protein & Cell 2013年第12期4卷 932-941页

作者： Yuchen Liu Luming Zhao Dameng Li Yuan Yin Chen-Yu Zhang Jing Li Yujing Zhang Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical BiotechnologySchool of Life SciencesNanjing UniversityNanjing 210093China Oncology Institute Fourth Affiliated Hospital of Suzhou UniversityWuxi 214062China

Tumor-associated macrophages(TAMs)mostly exhibit M2-like(alternatively activated)properties and play posi-tive roles in angiogenesis and *** en-dothelial growth factor(vegf)is a key angiogenic *** tumor development,TAMs secrete vegf and other factors to promote angiogenesis;thus,anti-treatment against TAMs and vegf can repress cancer development,which has been demonstrated in clinical trials and on an experimental *** the present work,we show that miR-150 is an oncomir because of its promotional effect on ***-150 targets TAMs to up-regulate their secre-tion of vegf in *** the utilization of cell-derived vesicles,named microvesicles(MVs),we transferred an-tisense RNA targeted to miR-150 into mice and found that the neutralization of miR-150 down-regulates miR-150 and vegf levels in vivo and attenuates ***-fore,we proposed the therapeutic potential of neutralizing miR-150 to treat cancer and demonstrated a novel,natu-ral,microvesicle-based method for the transfer of nucleic acids.

关键词： microvesicle miR-150 tumorigenesis vegf neutralization attenuation

A self-assembling peptide targeting vegf receptors to inhibit angiogenesis

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Chinese Chemical Letters 2020年第12期31卷 3153-3157页

作者： Shifang Wen Kuo Zhang Yuan Li Jiaqi Fan Ziming Chen Jjingping Zhang Hao Wang Lei Wang Faculty of Chemistry Northeast Normal UniversityChangchun 130024China Laboratory for Biomedical Effects of Nanomaterials and Nanosafety National Center for Nanoscience and Technology(NCNST)Beijing 100190China

Vascular endothelial growth factor(vegf)-vascular endothelial growth factor receptor(vegfR)pathways are essential in tumor angiogenesis,growth and *** on anti-angiogenic therapy have been mostly focused on the blockage of vegf-vegfR *** report an extracellularly transformable peptide-based nanomaterial to develop artificial extracellular matrix(ECM)-like networks for high-efficient blockage of natural vegf-vegfR *** transformable peptide-based nanomaterial transforms from nanoparticles into nanofibers upon binding to vegfR in *** addition,the transformable peptide-based nanomate rial forms ECM-like fibrous netwo rks on vegfR overexpressed cells,inhibiting the vegf-vegfR interactions and the subsequent *** tube formation is reduced by nearly 85.1% after *** strategy shows excellent potential for anti-angiogenesis,and inhibition of tumor invasion and metastasis.

关键词： Self-assembly Peptide vegf Angiogenesis Biomimetic

Over-expression of vegf in Marrow Stromal Cells Promotes Angiogenesis in Rats with Cerebral Infarction via the Synergistic Effects of vegf and Ang-2

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2012年第5期32卷 724-731页

作者：赖天宝李嫚郑丽芳宋艳玲徐小丽郭远瑾张远张宗胜梅元武 Department of Neurology Union HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Neurology Zhongshan HospitalXiamen University Department of Neurology Tianyou HospitalWuhan University of Science and Technology Department of Neurology Central Hospital of Weinan City Department of Hematology Tongji Medical CollegeHuazhong University of Science and Technology

This study explored whether the transplantation of modified marrow stromal cells (MSCs) has angiogenic effects in a left middle cerebral artery occlusion infarction/reperfusion (MCAO I/R) rat model and preliminarily examined the mechanism of angiogenesis following cerebral *** were isolated by using a direct adherent method and *** endothelial growth factor (vegf) was transfected into MSCs by employing the liposome *** transfection efficiency was measured by the optical density *** protein expression of vegf gene before and after transfection was measured by Western *** rat model of transient occlusion of the left middle cerebral artery was established by using an approach of intra-luminal *** (TTC) and HE staining were performed to observe the cerebral *** were used to measure the levels of vegf in the rat cerebral *** expression patterns of angiopoietin-2 (Ang-2) and CD34 in cells surrounding the area of infarction were immunohistochemistrically ***-2 protein expression in the tissue surrounding the area of infarction was measured by Western *** expression in the MSCs increased after transfection at a rate of approximately 28%±3.4%.ELISA showed that the expression of vegf in the cerebral tissue was significantly increased after induction of infarction,peaking on the 4th day and decreasing to the levels of the sham surgery group (normal) within 7 to 10 *** vegf level was significantly higher at each time point in the vegf-MSC and MSC groups compared to the model ***,the vegf level was higher in the vegf-MSC group than in the MSC group and stayed relatively high until the 10th *** immunohistochemical results showed that 10 days after the infarction,the number of Ang-2 and CD34-expressing cells in the area surrounding the infarction was significantly higher in the vegf-MSC group and the MSC group compared to the model ***,the

关键词： vegf marrow stromal cells cerebral infarction angiogenesis

Nerve growth factor alleviates cerebral infarction and neurologic deficits by regulating vegf,SDF-1 and S100A12 expression through PI3K pathway

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BIOCELL 2019年第3期43卷 183-189页

作者： ZHENG LI ZHONGLE ZHAO PENG ZHANG Department of Neurosurgery People’s Hospital of Deyang CityDeyangChina Department of Emergency the Forth People’s Hospital of ShaanxiXi’anChina Department of Emergency First People’s Hospital of NantongNantongChina

Stroke remains the leading cause of death and disability worldwide,which destroys the quality of patients’lives and thus is becoming a heavy burden to the ***,the current therapeutic approaches are far from *** objective of this study is to elucidate the impact of nerve growth factor(NGF)on the brain damage induced by cerebral ischemia and its potential molecular *** cerebral artery occlusion(MCAO)rats were used as animal models and neurological functions were evaluated by modified Neurological Severity Score(NSS).Brain cell apoptosis was analyzed by TUNEL-positive staining while brain infarct size was determined according to 2% 2,3,5-triphenyltetrazolium chloride(TCC)staining *** receiving NGF demonstrated significantly alleviated brain damage,reflected by a substantial improvement in the neurobehavioral outcome,a decrease in brain cell apoptosis and shrinkage of brain infarct *** analysis revealed a markedly elevated circulating vascular endothelial growth factor(vegf)and stromal cell-derived factor 1(SDF-1)levels as well as a significant downregulation of SA10012 expression in NGF treated group compared with the untreated ***,the protective effect of NGF on cerebral ischemic injury was abolished in rats treated with both NGF and PI3K inhibitors,indicating that phosphoinositide-3-kinase(PI3K)signaling is essential for NGF *** conclusion,NGF treatment might be a potential therapeutic approach against cerebral infarction by downregulating SA10012 expression and upregulating vegf,SDF-1 in a PI3K signaling dependent manner.

关键词： Cerebral infarction Nerve growth factor PI3K SA10012 SDF-1 vegf

Expression of LIF,vegf,CD57 and CD68 after the transfer of rat embryos to mouse uteri

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Chinese Science Bulletin 2003年第6期48卷 542-548页

作者： DAIBojie CAOYujing 等 StateKeyLaboratoryofReproductiveBiology InstituteofZoologyChineseAcademyofSciencesBeijing100080China StateKeyLaboratoryofReproductiveBiology InstituteofZoologyChineseAcademyofSciencesBeijing10008

The high failure rate of interspecific pregnancy is a major obstacle to the successful interspecific cloning of mammals. To investigate the reasons for the failure of inter-specfic pregnancy between rats and mice, we transferred rat blastocysts into mouse uteri on the third day of pseudopreg-nancy (D3). Our previous study showed that intact rat em-bryos could still be observed in mouse uteri on D9. In the present study, we found that expression of CD57 and CD68 increased significantly at the maternal-fetal interface fol-lowing the transfer of rat embryos. Similarly, Leukaemia inhibitory factor (LIF) expression increased, but vascular endothelial growth factor (vegf) expession decreased. In a co-culture system, the percentage of rat ectoplacental cones (EPCs) with adhesion and outgrowth and outgrowth area on mouse uterine decidual cells were less than that of mouse EPCs. These results indicate that an increase in the immu-nological rejection response and a decrease in the invasive-ness of rat embryos may be important reasons for the failure of interspecific pregnancy between rat and mouse.

关键词：大鼠胚胎小白鼠子宫胚胎移植 LIF vegf CD57 CD68 基因表达

Functions of vegf in female reproductive system

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Chinese Science Bulletin 2003年第3期48卷 217-222页

作者： QIANDong ZHUCheng StateKeyLaboratoryofReproductiveBiology InstituteofZoologyChineseAcademyofSciencesBeijing100080China StateKeyLaboratoryofReproductiveBiology InstituteofZoologyChineseAcademyofSciencesBeijing10008

As a homodimeric glycoprotein, vascular en- dothelial growth factor (vegf) is a highly specific mitogen of vascular endothelial cells. It can induce proliferation and migration, and inhibit apoptosis of endothelial cell. vegf is involved in many processes in the female reproductive sys-tem, such as ovulation, periodical changes of endometrium, embryo implantation and development. vegf plays impor-tant roles in some reproductive diseases, including pree-clampsia and fetal hypoevolutism in uterus. Based on our studies on angiogenesis and its relevant factors in the female reproductive system these years, the functions of vegf in female reproductive system are reviewed, and the research prospect and application of vegf are also discussed.

关键词： vegf 血管内皮生长因子受体雌性生殖系统生物学作用血管生成生殖医学

Effect of vegf,P53 and telomerase on angiogenesis of gastric carcinoma tissue

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Asian Pacific Journal of Tropical Medicine 2014年第4期7卷 293-296页

作者： Yan-Fang Yu Yong Zhang Na Shen Rui-Ying Zhang Xin-Qing Lu Affiliated Hospital of Hebei University of Engineering

Objective:To investigate the effect of vascular endothelial growth factor(vegf),P53 and telomerase on angiogenesis in gastric carcinoma ***:A total of 95 surgical resection samples of gastric cancer tissue after pathological diagnosis are collected to observe the vegf,P53 and telomerase expression using immunohistochemical *** between their expression and its influence on angiogenesis in gastric carcinoma tissue were ***:Microvascular density(MVD)and the expression of vegf,P53 and telomerase were positively *** of vegf and P53 protein were related to tumor type and lymph metastasis,and also a correlation was observed between P53 and *** telomerase expression had no correlation with vegf,and ***:vegf angiogenesis has a angiogenesis promoting effect on gastric cancer tissue development and plays an important role in tumor generation and *** P53 promotes the tumor angiogenesis generation by adjusting *** has a certain role in promoting activity of angiogenesis through different way rather than P53.

关键词： vegf P53 Telomerase Angiogenesis Gastric carcinoma