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Indication of the Expression of Transgene in rice Plant Based on Hyperspectral Remote Sensing Technique Ⅱ——Growth Monitoring of Samples in the Contrast Experiment

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光谱学与光谱分析 2011年第6期31卷 1621-1626页

作者： LI Ru CHEN Jin-song YUAN Ding-yang LIN Hui TAN Yan-ning YUE Yue-min Institute of Space and Earth Information Science The Chinese University of Hong KongHong KongChina China National Hybrid rice R＆D Center/ Hunan Hybrid rice Research Center Changsha 410125China Institute of Subtropical Agriculture Chinese Academy of SciencesChangsha 410125China

Since the complication of monitoring and evaluating the problems about the transgenic expression and its impacts on the receptor in the transgenic crop breeding and other relevant evaluated works,the authors in the present work tried to assess the differences of spectral parameters of the transgenic rice in contrast with its parent group quantitatively and qualitatively,fulfilling the growth monitoring of the transgenic *** spectral parameters(spectral morphological characteristics and indices) chosen are highly related to internal or external stresses to the receipts,and thus could be applied as indicators of biophysical or biochemical processes changes of *** ASD portable field spectroradiometer with high-density probe,fine foliar spectra of 8 groups were *** analyzing spectral angle and continuum removal,the spectral morphological differences and their locations of sample spectra were found which could be as auxiliary priori knowledge for quantitative *** investigating spectral indices of the samples,the quantitative differences of spectra were revealed about foliar chlorophyll a+b and carotenoid *** this study both the spectral differences between transgenic and parent groups and among transgenic groups were *** results show that hyperspectral technique is promising and a helpful auxiliary tool in the study of monitoring the transgenic crop and other relevant *** this technique,quantitative and qualitative results of sample spectra could be provided as prior knowledge,as certain orientation,for laboratory professional advanced transgenic breeding study.

关键词： transgenic rice Hyperspectral remote sensing Growth monitoring

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Transformation of Pea Lectin Gene and Parasponia Haemoglobin Gene into rice and Their Expressions

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Acta Botanica Sinica 2001年第3期43卷 267-274页

作者：张静娴王忆平沈世华王逸群高越峰单雪琴荆玉祥中国科学院植物研究所北京100093 北京大学生命科学学院北京100871

Lectin and leghemoglobin in legumes play the important roles, respectively, in recognition of host plants to their rhizobial bacteria, and lowering the oxygen partial pressure around bacteroids and protecting nitrogenase from oxygen in symbiotic nitrogen-fixing nodules. In order to extend the host range of the rhizobial bacteria and to make them fix nitrogen in non-legumes, pea lectin gene (pl) and Parasponia hemoglobin gene ( phl,) have been constructed into a plant expression vector (pCBHUL) and the vector pCBHUL was introduced into rice calli from immature young embryos by particle bombardment. After the calli were regenerated into plantlets on the resistant-selecting media containing hygromycin, they were identified by PCR and Southern blot hybridization. It was indicated that the pi and phb genes were integrated into nucleic genome of the transformed rice plants. GUS activity and the product of the pi gene were determined by GUS staining, Western blot and in situ hybridization at translational level. Eighteen out of 40 plants resistant to hygromycin were positively identified by PCR analysis with the rate of 45%. The pi gene was expressed in 3 out of 18 plants with 17% and 7.5% in 40 plants. The results may provide a clue for exploring whether Rhizobium leguminosarum by. viceae could extend its host range and make the transgenic rice plants have the possibility of being symbiotic, or associative to nitrogen fixation.

关键词： Parasponia hemoglobin gene pea lectin gene particle bombardment transformation gene expression transgenic rice

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Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第10期16卷 824-831页

作者： Qi-chao ZHAO Ming-hong LIU Xian-wen ZHANG Chao-yang LIN Qing ZHANG Zhi-cheng SHEN State Key Laboratory of rice Biology Institute of Insect Sciences Zhejiang University

Insect resistance and glyphosate tolerance have been two of the most important traits in the genetic improvement of various crops. In this study, two Bacillus thuringiensis （Bt） insecticidal genes, CrylAc and Cryllg, and a modified glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase （EPSPS） gene （GIO） were combined into a single transferred DNA （T-DNA） fragment and introduced into rice by Agrobacterium-mediated transformation. A transgenic line with single-copy T-DNA insertion named GAI-14 was found to be highly resistant to striped stem borer and rice leaf roller, and tolerant to glyphosate. Analysis of T-DNA border sequence suggested that the transgenes were inserted at the chromosome 3 and appeared to have not interrupted any known or putative genes. A field trial observed no significant difference in the basic agronomic traits between GAI-14 and the recipient rice.

关键词： Bt gene stacking Bt resistance management EPSPS transgenic rice

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transgenic plants for allergen-specific immunotherapy

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World Journal of Immunology 2014年第3期4卷 141-148页

作者： Tomoe Nishimura Mayumi Saeki Osamu Kaminuma Fumio Takaiwa Takachika Hiroi Allergy and Immunology Project Tokyo Metropolitan Institute of Medical ScienceTokyo 156-8506Japan Genetically Modified Organism Research Center National Institute of Agrobiological SciencesIbaraki 305-8602Japan

Allergen-specific immunotherapy （IT） is an effective treatment for allergic diseases. Although subcutane-ous and sublingual ITs are currently used, safer, easier, and more effective IT is under development. Induction of immune tolerance by oral administration of allergen has been proven, though oral IT has not been applied clinically. It is mainly because a large amount of puri-fied allergen is required to induce oral tolerance. To overcome this problem, plants, peculiarly rice, have been investigated as allergen vehicles for oral IT. rice can store a considerable amount of expressed allergen in its seeds and the accumulated allergen is stable and resistant to gastrointestinal digestion. Therefore, we have developed transgenic rice seeds （Tg rice） in which major epitopes of cedar pollen or house dust mites are expressed. We are establishing Tg rice with dem-onstrated effcacy in murine models of allergic rhinitis and bronchial asthma by oral administration at practical doses. In addition, the amount, distribution, and aller-genicity of the expressed allergen have been improved in our Tg rice. rice-based oral IT is a promising new concept in IT for the treatment of allergic diseases.

关键词： Allergic disease Asthma Oral immunother-apy Rhinitis transgenic rice

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Drought Tolerance Enhancement with Co-Overexpression of DREB2A and APX in Indica rice (<i>Oryza sativa</i>L.)

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American Journal of Plant Sciences 2021年第2期12卷 234-258页

作者： Jagtap Sandhya Talakayala Ashwini Ravula Manisha Mahankali Vinodha Ankanagari Srinivas Department of Genetics & Biotechnology Osmania University Hyderabad India

rice is a major cereal crop providing food and energy to more than half of world’s population and drought is a challenging abiotic stress limiting rice production. Engineering drought tolerance trait is a major bottle neck because of multigenic control and complex nature. Two promising candidate genes utilized in engineering drought tolerance include DREB2A transcription factor (a master regulator of downstream stress inducible genes) and APX (an important ROS scavenging enzyme). Overexpression of DREB genes has shown encouraging results but with a negative impact on plant morphology and production. Moreover, co-expression of DREB2A and APX genes’ influence on drought stress has not been studied. Hence, in the present study, overexpression of single genes DREB2A or APX and co-expression of these genes were studied for enhancement of drought tolerance in indica rice. Both genes under control of CaMV 35S promoter were transferred by Agrobacterium transformation into rice variety BPT5204 popular for slender grains in South India. Confirmation of T-DNA integration into rice genome was done with PCR analysis of transgenes. Homozygous transgenic lines of DREB2A, APX and DREB2A-APX generated in T3 generation were evaluated for

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Establishment of a rice transgene flow model for predicting maximum distances of gene flow in southern China

Establishment of a rice transgene flow model for predicting ...

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粮食安全与现代农业气象业务发展——2008年全国农业气象学术年会

作者： Kemin Yao~1,Ning Hu~1,Wanlong Chen~1,Renzhong Li~1,Qianhua Yuan~2,Feng Wang~3,Qian Qian~4,Shirong Jia~5 1 College of Applied Meteorology,Nanjing University of Information Science and Technology,Nanjing,210044,China 2 College of Life Science and Agriculture,Hainan University,Haikou 570228,China 3 rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,510640,China 4 National rice Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou,310006,China 5 Bioteehnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China.

Both rice pollen flow and gene flow model have been established based on the Gaussian Plume Model,the 3 locations×3 years field experimental data of transgene flow to common rice cultivars(Oryza sativa L.),male sterile(ms) lines and common wild rice(*** L.),and the 32 years historical meteorology data during the rice flowering period collected from 38 meteorological stations in southern China. A concept of gene flow coefficient(GFC) is proposed,that is the ratio of the transgene flow frequency(G%) obtained from field experiments against the aggregated pollen dispersal ratio(P) or frequency(P%) calculated based on the pollen flow model. The maximum distances of gene flow(MDGF) to traditional rice cultivars,ms lines,and common wild rice either at a threshold value of 1.0%or 0.1%are determined. The MDGF and its spatial distribution in southern China show that the gene flow pattern is significantly affected by the monsoon climate,the topography,and the outcrossing ability of recipients. We believe that the information provided in this study will be useful for the risk assessment of transgenic rice in other rice growing regions.

关键词： transgenic rice Pollen flow model Gene flow coefficient Gene flow model Cultivated rice Male sterile lines Common wild rice.

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A Glycosyl-phosphatidyl Inositol Anchored Protein Gene,OsGAP1,is Involved in Early Root Elongation and Normal Growth of Oryza Sativa

A Glycosyl-phosphatidyl Inositol Anchored Protein Gene,OsGAP...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： LIU Bing1, FENG Dong-ru1, WANG Hong-bin1* and WANG Jin-fa1 1The State Key Laboratory of Biocontrol and The Key Labrotary of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China

＜正＞Glycosyl-phosphatidyl inositol （GPI） anchored proteins are a class of plasma membrane-localized proteins with important functions in eukaryotic cells. A putative GPI anchored protein gene OsGAP1 was isolated f... 详细信息

关键词： GPI anchored proteins RNA interference overexpression root development transgenic rice

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Stress Inducible Truncated AtSOS1 Gene Expression in rice Enhances Salt Tolerance

Stress Inducible Truncated AtSOS1 Gene Expression in Rice En...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Rui Wang1, Meng Cui1, Zhengge Zhu1, 2*, Ray Wu2 1 College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050016, China 2 Department of Molecular Biology & Genetics, Cornell University, Ithaca, NY 14853, USA

＜正＞The Arabidopsis AtSOS1 gene encodes a plasma membrane Na+/H+ antiporter which is essential for improving NaCl tolerance of plants. In this study, the AtSOS1 and truncated AtSOS1 gene were expressed under the stress

关键词： AtSOS1 salt stress transgenic rice

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Over-Expression of a Transcription Factor OsDREB2A （p） Gene in rice Enhances Drought or Salt Tolerance

Over-Expression of a Transcription Factor OsDREB2A （p） Gen...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Ray Wu Department of Molecular Biology ＆ Genetics Cornell University Ithaca NY 14853 USA

＜正＞The plant DREBs/CBFs transcription factors play a critical roles in plant development and abiotic stress responses. In this study, a putative rice OsDREB2A gene was expressed under the stress inducible promoter

关键词： transcription factors OsDREB2A drought stress salt stress transgenic rice

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Control of Mythimna Separata using RNAi technology

Control of Mythimna Separata using RNAi technology

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全国农业生物化学与分子生物学第十五届学术研讨会

作者： Wenhua Bao Hada Wuriyanghan 内蒙古大学生命科学学院

Mythimna Separata is major agricultural pests that cause significant yield losses of crop plants each *** of double-stranded RNA(dsRNA) directed against suitable insect target genes in transgenic plants has been shown to give protection against pests through plant-mediated RNA interference(RNAi).Thus,as a potential effective strategy for insect pest management in agricultural practice,RNAi for Mythimna separata control has received close attention in recent *** propose here to develop Mythimna separata-resistant plants using RNAi *** primary hypothesis is that if we can identify and deliver to Mythimna separata interfering RNAs that down-regulate expression of Mythimna separata genes such as Chitinase genes which are essential for their growth and development,they will result in a debilitating *** do this,we designed and carried out the following *** used different sources of RNAi effectors to achieve knockdown of Mse-Chi genes and caused lethal *** rice expressing the hairpin dsRNAs was verified by southern blot and *** the transgenic rice was fed to Mythimna separata,target Chitinase genes transcript levels were substantially decreased,also resulting in retarded larval growth and increased mortality.

关键词： Mythimna separata RNAi Chitinase gene transgenic rice

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