Control of Mythimna Separata using RNAi technology
作者单位：内蒙古大学生命科学学院
会议名称：《全国农业生物化学与分子生物学第十五届学术研讨会》
会议日期：2016年
学科分类：09[农学] 0904[农学-植物保护] 090402[农学-农业昆虫与害虫防治]
关 键 词：Mythimna separata RNAi Chitinase gene transgenic rice
摘 要：Mythimna Separata is major agricultural pests that cause significant yield losses of crop plants each *** of double-stranded RNA(dsRNA) directed against suitable insect target genes in transgenic plants has been shown to give protection against pests through plant-mediated RNA interference(RNAi).Thus,as a potential effective strategy for insect pest management in agricultural practice,RNAi for Mythimna separata control has received close attention in recent *** propose here to develop Mythimna separata-resistant plants using RNAi *** primary hypothesis is that if we can identify and deliver to Mythimna separata interfering RNAs that down-regulate expression of Mythimna separata genes such as Chitinase genes which are essential for their growth and development,they will result in a debilitating *** do this,we designed and carried out the following *** used different sources of RNAi effectors to achieve knockdown of Mse-Chi genes and caused lethal *** rice expressing the hairpin dsRNAs was verified by southern blot and *** the transgenic rice was fed to Mythimna separata,target Chitinase genes transcript levels were substantially decreased,also resulting in retarded larval growth and increased mortality.