检索结果-南通市图书馆

SCancerRNA:Expression at the single-cell Level and Interaction Resource of Non-coding RNA Biomarkers for Cancers

Genomics, Proteomics & Bioinformatics 2024年第3期22卷 125-131页

作者： Hongzhe Guo Liyuan Zhang Xinran Cui Liang Cheng Tianyi Zhao Yadong Wang Faculty of Computing Harbin Institute of TechnologyHarbin 150001China College of Bioinformatics Science and Technology Harbin Medical UniversityHarbin 150001China School of Medicine and Health Harbin Institute of TechnologyHarbin 150001China

Non-coding RNAs(ncRNAs)participate in multiple biological processes associated with cancers as tumor suppressors or oncogenic *** to their high stability in plasma,urine,and many other fluids,ncRNAs have the potential to serve as key biomarkers for early diagnosis and screening of *** cancer progression,tumor heterogeneity plays a crucial role,and it is particularly important to understand the gene expression patterns of individual *** the development of single-cell RNA sequencing(scRNA-seq)technologies,uncovering gene expression in different cell types for human cancers has become feasible by profiling transcriptomes at the cellular ***,a well-organized and comprehensive online resource that provides access to the expression of genes corresponding to ncRNA biomarkers in different cell types at the single-cell level is not available ***,we developed the SCancerRNA database to summarize experimentally supported data on long ncRNA,microRNA,PIWI-interacting RNA,small nucleolar RNA,and circular RNA biomarkers,as well as data on their differential expression at the cellular ***,we collected biological functions and clinical applications of biomarkers to facilitate the application of ncRNA biomarkers to cancer diagnosis,as well as the monitoring of progression and targeted *** also allows users to explore interaction networks of different types of ncRNAs,and build computational models in the *** is freely accessible at http://***/BioMarker.

关键词： Non-coding RNA Biomarker Cancer single cell Interaction network

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Recent progress in co-detection of single-cell transcripts and proteins

引用

Nano Research 2024年第1期17卷 132-150页

作者： Shanqing Huang Qian Fan Yidi Wang Zhi Huang Weixiong Shi Yanli Gong Ting Yang Jie Wang Lingling Wu Chaoyong Yang Discipline of Intelligent Instrument and Equipment College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China Institute of Molecular Medicine Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China Department of General Surgery Xiang’an Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361101China Research Center for Analytical Sciences Department of ChemistryCollege of SciencesNortheastern UniversityShenyang 110819China

cellular heterogeneity is a universal property of living systems,and the interrogation of single cells facilitates in-depth understanding of distinct cellular states and functions in various biological ***-analysis of transcripts and proteins from the same single cells opens the way to decipher complex RNA regulatory frameworks and phenotypes,facilitating the understanding of cellular fate and function regulations,discovery of novel cell types,and construction of a high-resolution cell ***,we review the state-of-art advances in the development of methodologies for co-analysis of single-cell transcripts and ***,imaging-based methods are summarized with particular emphasis on optical and mass spectrometry ***,sequencing-based approaches for high-throughput and sensitive co-analysis of single-cell transcripts and proteins are described,including droplet-,microwell-,and split-pool-based ***,combined methods with more flexibility and universality are *** methods commonly employ different strategies or reactions to convert transcripts and proteins of single cells into distinct signals simultaneously,which can be detected by different instruments or ***,some perspectives on the future challenges and development trends in this field are presented.

关键词： single cell optical imaging mass spectrometry imaging single-cell RNA sequencing(scRNA-seq) co-detection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Sex-biased single-cell genetic landscape in mice with autism spectrum disorder

引用

Journal of Genetics and Genomics 2024年第3期51卷 338-351页

作者： Qian Zhang Yongjie Wang Jie Tao Ruixue Xia Yijie Zhang Zhirui Liu Jiwei Cheng Department of Neurology and Central Laboratory Putuo HospitalShanghai University of Traditional Chinese MedicineShanghai 200062China School of Pharmacy Hangzhou Normal UniversityHangzhouZhejiang 311121China Department of Respiratory and Critical Care Medicine Henan University Huaihe HospitalKaifengHenan 475099China

Autistic spectrum disorder(ASD)is a male-biased,heterogeneous neurodevelopmental disorder that affects approximately 1%e2%of the *** exposure to valproic acid(VPA)is a recognized risk factor for ASD,but the cellular and molecular basis of VPA-induced ASD at the single-cell resolution is ***,we aim to compare the cellular and molecular differences in the hippocampus between male and female prenatal mice with ASD at the single-cell transcriptomic *** transcriptomes of more than 45,000 cells are assigned to 12 major cell types,including neurons,glial cells,vascular cells,and immune *** type-specific genes with altered expression after prenatal VPA exposure are analyzed,and the largest number of differentially expressed genes(DEGs)are found in neurons,choroid plexus epithelial cells,and *** microglia,several pathways related to inflammation are found in both males and females,including the tumor necrosis factor(TNF),nuclear factor kappa B(NF-kB),toll-like receptor(TLR),and mitogen-activated protein kinase(MAPK)signaling pathways,which are important for the induction of autistic-like ***,we note that several X-linked genes,including Bex1,Bex3,and Gria3,were among the male-specific DEGs of *** pioneering study describes the landscape of the transcriptome in the hippocampus of autistic *** elucidation of sexual differences could provide innovative strategies for the prevention and treatment of ASD.

关键词： Autistic spectrum disorder(ASD) Sex single cell Neurons Microglia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in single-cell sequencing technology in microbiome research

引用

Genes & Diseases 2024年第4期11卷 201-219页

作者： Yinhang Wu Jing Zhuang Yifei Song Xinyi Gao Jian Chu Shuwen Han Huzhou Central Hospital Affiliated Central Hospital Huzhou UniversityHuzhouZhejiang 313000China Key Laboratory of Multiomics Research and Clinical Transformation of Digestive Cancer of Huzhou HuzhouZhejiang 313000China The Fifth Affiliated Clinical Medical College of Zhejiang Chinese Medical University HangzhouZhejiang 313000China Zhejiang Provincial People's Hospital and Affiliated People's Hospital Hangzhou Medical CollegeHangzhouZhejiang 310000China

With the rapid development of histological techniques and the widespread applica-tion of single-cell sequencing in eukaryotes,researchers desire to explore individual *** and functional expression,which deepens our understanding of *** this review,the history of the development of microbial detection technologies was revealed and the difficulties in the application of single-cell sequencing in microorganisms were dissected as ***,the characteristics of the currently emerging microbial single-cell sequencing(Microbe-seq)technology were summarized,and the prospects of the application of Microbe-seq in microorganisms were distilled based on the current development *** its mature development,the Microbe-seq technology was still in the optimization stage.A retrospective study was conducted,aiming to promote the widespread application of single-cell sequencing in microorganisms and facilitate further improvement in the technol-ogy.

关键词： Bacterialantibiotic resistance Host immunity Host-phage interaction Microbial single-cell sequencing Microorganisms single cell single-cell sequencing technology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

single cell DEGENERATE OLIGONUCLEOTIDE PRIMER-PCR AND COMPARATIVE GENOMIC HYBRIDIZATION WITH MODIFIED CONTROL REFERENCE

引用

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2001年第3期2卷 79-82页

作者：金帆 Women’s Hospital College of Medicine Zhejiang University Hangzhou China Department of Obstetrics and Gynecology University of Adelaide Australia

For investigating the possibility of applying degenerate oligonucleotide primer PCR (DOP\|PCR) and comparative genomic hybridization (CGH) technique to analyses of genomic genetics in a single cell, the whole genomic DNA of a single cell with XX, XY, XO, XXY, +13 or +21 was amplified by DOP\|PCR. single cell DOP\|PCR CGHs with conventional and modified control references, the genomic DNA and a single cell DOP\|PCR product from normal male, were carried out respectively. The results showed that the average profile of the fluorescence intensity ratio in CGH with the genomic DNA as reference fluctuates much and that the standard deviation in about 30% haploid is beyond the normal limits. False positive hyper\|representation was found to exist in X chromosome while trisomy 13 and 21 were not detected. However, the distributions of the mean and the standard deviation of the ratio in the CGH with DOP\|PCR product as reference were quite acceptable. The copy number changes of chromosome X,Y,13 and 21 were revealed. Those results suggested that there is unrandom unequal amplification in a single cell DOP\|PCR. Using a single DOP\|PCR product as reference can decrease its influence on CGH. single cell DOP\|PCR\|CGH and its application in the genetic analyses of preimplantation embryo or fetal cell in maternal blood may be possible.

关键词： degenerate oligonucleotide primer PCR comparative genomic hybridization single cell

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Sensitive determination of inosine RNA modification in single cell by chemical derivatization coupled with mass spectrometry analysis

引用

中国化学快报:英文版 2023年第10期34卷 98-101页

作者： Wan-Bing Tao Neng-Bin Xie Qing-Yun Cheng Yu-Qi Feng Bi-Feng Yuan College of Chemistry and Molecular Sciences Department of Radiation and Medical OncologyZhongnan Hospital of Wuhan UniversityWuhan UniversityWuhan 430071China School of Public Health Wuhan UniversityWuhan 430071China Research Center of Public Health Renmin Hospital of Wuhan UniversityWuhan 430060China Cancer Precision Diagnosis and Treatment and Translational Medicine Hubei Engineering Research Center Zhongnan Hospital of Wuhan UniversityWuhan Research Center for Infectious Diseases and CancerChinese Academy of Medical SciencesWuhan 430071China

Inosine is a vital RNA modification across three kingdoms of *** has been demonstrated that inosine plays important roles in modulation of the fate of *** the current study,we developed a highly sensitive method to determine inosine in a single cell by N-cyclohexyl-N’-β-(4-methylmorpholinium)ethylcarbodiimide p-toluenesulfonate(CMCT)derivatization in combination with mass spectrometry *** results showed that the detection sensitivity of inosine was increased by 556-fold after CMCT derivatization,with the limit of detection(LOD)being 4.5 *** the established method,we could detect inosine from 13.0 pg of total RNA of HEK293T ***,inosine in RNA from a single cell could also be clearly detected due to the improved detection ***,we found the level of inosine in RNA of sleep-deprived mice was significantly increased compared to the control mice,indicating that inosine is associated with sleep behavior and might be a potential indicator of sleep *** together,the chemical derivatization coupled with mass spectrometry analysis offers a valuable tool in determination of endogenous RNA modifications in a single cell,which should benefit the functional study of RNA modification in rare clinical samples.

关键词： Inosine RNA modification CMCT Derivatization Mass spectrometry single cell

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Tunable microfluidic chip for single-cell deformation study

引用

Nanotechnology and Precision Engineering 2023年第2期6卷 25-32页

作者： Ruiyun Zhang Xuexin Duan Shuaihua Zhang Wenlan Guo Chen Sun Ziyu Han College of Precision Instrument and Opto-electronics Engineering Tianjin UniversityTianjin 300072China State Key Laboratory of Precision Measuring Technology and Instruments Tianjin UniversityTianjin 300072China

Microfluidic phenotyping methods have been of vital importance for cellular characterization,especially for evaluating single *** order to study the deformability of a single cell,we devised and tested a tunable microfluidic chip-based method.A pneumatic polymer polydimethylsiloxane(PDMS)membrane was designed and fabricated abutting a single-cell trapping structure,so the cell could be squeezed controllably in a lateral *** contour changes under increasing pressure were recorded,enabling the deformation degree of different types of single cell to be analyzed and compared using computer *** provides a new perspective for studying mechanical properties of cells at the single cell level.

关键词： Microfluidics cell deformation single cell Computer vision

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

single-cell profiling reveals Müller glia coordinate retinal intercellular communication during light/dark adaptation via thyroid hormone signaling

引用

蛋白质与细胞:英文版 2023年第8期14卷 603-617页

作者： Min Wei Yanping Sun Shouzhen Li Yunuo Chen Longfei Li Minghao Fang Ronghua Shi Dali Tong Jutao Chen Yuqian Ma Kun Qu Mei Zhang Tian Xue Division of Life Sciences and Medicine Department of OphthalmologyThe First Affiliated Hospital of USTCUniversity of Science and Technology of ChinaHefei 230026China Hefei National Research Center for Physical Sciences at the Microscale Neurodegenerative Disorder Research CenterCAS Key Laboratory of Brain Function and DiseaseUniversity of Science and Technology of ChinaHefei 230026China Division of Life Sciences and Medicine School of Life SciencesUniversity of Science and Technology of ChinaHefei 230026China Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghai 200031China Institute for Stem cell and Regeneration Chinese Academy of SciencesBeijing 100864China

Light adaptation enables the vertebrate visual system to operate over a wide range of ambient *** of phototransduction in photoreceptors is considered a major mechanism underlying light ***,various types of neurons and glial cells exist in the retina,and whether and how all retinal cells interact to adapt to light/dark conditions at the cellular and molecular levels requires systematic ***,we utilized single-cell RNA sequencing to dissect retinal cell-type-specific transcriptomes during light/dark adaptation in *** results demonstrated that,in addition to photoreceptors,other retinal cell types also showed dynamic molecular changes and specifically enriched signaling pathways under light/dark ***,Müller glial cells(MGs)were identified as hub cells for intercellular interactions,displaying complex cell‒cell communication with other retinal ***,light increased the transcription of the deiodinase Dio2 in MGs,which converted thyroxine(T4)to active triiodothyronine(T3).Subsequently,light increased T3 levels and regulated mitochondrial respiration in retinal cells in response to light *** cones specifically express the thyroid hormone receptor Thrb,they responded to the increase in T3 by adjusting light *** of the expression of Dio2 specifically in MGs decreased the light responsive ability of *** results suggest that retinal cells display global transcriptional changes under light/dark adaptation and that MGs coordinate intercellular communication during light/dark adaptation via thyroid hormone signaling.

关键词： single cell Müller glial cells intercellular communication light/dark adaptation thyroid hormone signaling

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Integration of Droplet Microfluidic Tools for single-cell Functional Metagenomics:An Engineering Head Start

引用

Genomics, Proteomics & Bioinformatics 2021年第3期19卷 504-518页

作者： David Conchouso Amani Al-Ma’abadi Hayedeh Behzad Mohammed Alarawi Masahito Hosokawa Yohei Nishikawa Haruko Takeyama Katsuhiko Mineta Takashi Gojobori Department of Industrial and Mechanical Engineering Universidad de las Américas PueblaPuebla 72810Mexico Computational Bioscience Research Center King Abdullah University of Science and TechnologyThuwal 23955-6900Saudi Arabia Biological and Environmental Sciences and Engineering Division King Abdullah University of Science and TechnologyThuwal 23955-6900Saudi Arabia Research Organization for Nano&Life Innovation Waseda UniversityTokyo 162-0041Japan Department of Life Science and Medical Bioscience Waseda UniversityTokyo 162-8480Japan Institute for Advanced Research of Biosystem Dynamics Waseda Research Institute for Science and EngineeringWaseda UniversityTokyo 169-8555Japan Computational Bio Big-Data Open Innovation Laboratory AIST-Waseda UniversityTokyo 169–0072Japan Computer Electricaland Mathematical Sciences and Engineering DivisionKing Abdullah University of Science and TechnologyThuwal 23955-6900Saudi Arabia

Droplet microfluidic techniques have shown promising outcome to study single cells at high ***,their adoption in laboratories studying“-omics”sciences is still irrelevant due to the complex and multidisciplinary nature of the *** facilitate their use,here we provide engineering details and organized protocols for integrating three droplet-based microfluidic technologies into the metagenomic pipeline to enable functional screening of bioproducts at high ***,a device encapsulating single cells in droplets at a rate of~250 Hz is described considering droplet size and cell ***,we expand on previously reported fluorescence-activated droplet sorting systems to integrate the use of 4 independent fluorescence-exciting lasers(i.e.,405,488,561,and 637 nm)in a single platform to make it compatible with different fluorescence-emitting *** this sorter,both hardware and software are provided and optimized for effortlessly sorting droplets at 60 ***,a passive droplet merger is also integrated into our pipeline to enable adding new reagents to already-made droplets at a rate of 200 ***,we provide an optimized recipe for manufacturing these chips using silicon dry-etching *** of the overall integration and the technical details presented here,our approach allows biologists to quickly use microfluidic technologies and achieve both single-cell resolution and high-throughput capability(>50,000 cells/day)for mining and bioprospecting metagenomic data.

关键词： Droplet microfluidics single cell Metagenomics Droplet sorter Biotechnology

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

single-cell approaches to investigate B cells and antibodies in autoimmune neurological disorders

引用

cellular & Molecular Immunology 2021年第2期18卷 294-306页

作者： Alicia Zou Sudarshini Ramanathan Russell C.Dale Fabienne Brilot Brain Autoimmunity Group Kids Neuroscience CentreKids Research at the Children’s Hospital at WestmeadSydneyNSWAustralia Discipline of Child and Adolescent Health Faculty of Medicine and HealthThe University of SydneySydneyNSWAustralia Brain and Mind Centre The University of SydneySydneyNSWAustralia School of Medical Sciences Discipline of Applied Medical ScienceFaculty of Medicine and HealthThe University of SydneySydneyNSWAustralia

Autoimmune neurological disorders,including neuromyelitis optica spectrum disorder,anti-N-methyl-D-aspartate receptor encephalitis,anti-MOG antibody-associated disorders,and myasthenia gravis,are clearly defined by the presence of autoantibodies against neurological *** these autoantibodies have been heavily studied for their biological activities,given the heterogeneity of polyclonal patient samples,the characteristics of a single antibody cannot be definitively *** review details the findings of polyclonal serum and CSF studies and then explores the advances made by single-cell technologies to the field of antibody-mediated neurological ***-resolution single-cell methods have revealed abnormalities in the tolerance mechanisms of several disorders and provided further insight into the B cells responsible for autoantibody ***,several factors,including epitope specificity and binding affinity,finely regulate the pathogenic potential of an autoantibody,and a deeper appreciation of these factors may progress the development of targeted immunotherapies for *** options.

关键词： B cells Recombinant antibodies Autoimmunity Central nervous system Neurological disorders Monoclonal single cell Sequencing Pathogenicity Epitope Affinity B cell tolerance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：