Single-cell profiling reveals Müller glia coordinate retinal intercellular communication during light/dark adaptation via thyroid hormone signaling

作     者：Min Wei Yanping Sun Shouzhen Li Yunuo Chen Longfei Li Minghao Fang Ronghua Shi Dali Tong Jutao Chen Yuqian Ma Kun Qu Mei Zhang Tian Xue Min Wei;Yanping Sun;Shouzhen Li;Yunuo Chen;Longfei Li;Minghao Fang;Ronghua Shi;Dali Tong;Jutao Chen;Yuqian Ma;Kun Qu;Mei Zhang;Tian Xue

作者机构：Division of Life Sciences and MedicineDepartment of OphthalmologyThe First Affiliated Hospital of USTCUniversity of Science and Technology of ChinaHefei 230026China Hefei National Research Center for Physical Sciences at the MicroscaleNeurodegenerative Disorder Research CenterCAS Key Laboratory of Brain Function and DiseaseUniversity of Science and Technology of ChinaHefei 230026China Division of Life Sciences and MedicineSchool of Life SciencesUniversity of Science and Technology of ChinaHefei 230026China Center for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesShanghai 200031China Institute for Stem Cell and RegenerationChinese Academy of SciencesBeijing 100864China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2023年第14卷第8期

页      面：603-617页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：supported by the National Key Basis Research and Development Program of China 国家自然科学基金 CAS Project for Young Scientists in Basic Research the Collaborative innovation Program of Hefei Science Center, CAS supported by the Anhui Provincial Natural Science Foundation Sponsored by Anhui Postdoctoral Scientific Program to D.T supported by "the Fundamental Research Funds for the Central Universities" 

主　　题：single cell Müller glial cells intercellular communication light/dark adaptation thyroid hormone signaling 

摘      要：Light adaptation enables the vertebrate visual system to operate over a wide range of ambient *** of phototransduction in photoreceptors is considered a major mechanism underlying light ***,various types of neurons and glial cells exist in the retina,and whether and how all retinal cells interact to adapt to light/dark conditions at the cellular and molecular levels requires systematic ***,we utilized single-cell RNA sequencing to dissect retinal cell-type-specific transcriptomes during light/dark adaptation in *** results demonstrated that,in addition to photoreceptors,other retinal cell types also showed dynamic molecular changes and specifically enriched signaling pathways under light/dark ***,Müller glial cells(MGs)were identified as hub cells for intercellular interactions,displaying complex cell‒cell communication with other retinal ***,light increased the transcription of the deiodinase Dio2 in MGs,which converted thyroxine(T4)to active triiodothyronine(T3).Subsequently,light increased T3 levels and regulated mitochondrial respiration in retinal cells in response to light *** cones specifically express the thyroid hormone receptor Thrb,they responded to the increase in T3 by adjusting light *** of the expression of Dio2 specifically in MGs decreased the light responsive ability of *** results suggest that retinal cells display global transcriptional changes under light/dark adaptation and that MGs coordinate intercellular communication during light/dark adaptation via thyroid hormone signaling.